{"id":"https://openalex.org/W1991369024","doi":"https://doi.org/10.1113/jphysiol.1996.sp021719","title":"Disulphonic stilbene block of cystic fibrosis transmembrane conductance regulator Cl\u2010 channels expressed in a mammalian cell line and its regulation by a critical pore residue.","display_name":"Disulphonic stilbene block of cystic fibrosis transmembrane conductance regulator Cl\u2010 channels expressed in a mammalian cell line and its regulation by a critical pore residue.","publication_year":1996,"publication_date":"1996-11-01","ids":{"openalex":"https://openalex.org/W1991369024","doi":"https://doi.org/10.1113/jphysiol.1996.sp021719","mag":"1991369024","pmid":"https://pubmed.ncbi.nlm.nih.gov/8930836","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1160856"},"language":"en","primary_location":{"id":"doi:10.1113/jphysiol.1996.sp021719","doi":"https://doi.org/10.1113/jphysiol.1996.sp021719","is_oa":false,"landing_page_url":"https://doi.org/10.1113/jphysiol.1996.sp021719","pdf_url":null,"source":{"id":"https://openalex.org/S2090548","display_name":"The Journal of Physiology","issn_l":"0022-3751","issn":["0022-3751","1469-7793"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1160856","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033237248","display_name":"Paul Linsdell","orcid":"https://orcid.org/0000-0001-6257-6273","observed_orcids":["https://orcid.org/0000-0001-6257-6273"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P Linsdell","raw_affiliation_strings":["Department of Physiology, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada. linsdell@medcor.mcgill.ca","Department of Physiology, McGill University, Montreal, Quebec, Canada.linsdell@medcor.mcgill.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada. linsdell@medcor.mcgill.ca","institution_ids":["https://openalex.org/I5023651"]},{"raw_affiliation_string":"Department of Physiology, McGill University, Montreal, Quebec, Canada.linsdell@medcor.mcgill.ca","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056371233","display_name":"John W. Hanrahan","orcid":"https://orcid.org/0000-0001-9080-2039","observed_orcids":["https://orcid.org/0000-0001-9080-2039"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"J W Hanrahan","raw_affiliation_strings":["Department of Physiology, McGill University, Montre\u0301al, Que\u0301bec, Canada. linsdell@medcor.mcgill.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, McGill University, Montre\u0301al, Que\u0301bec, Canada. linsdell@medcor.mcgill.ca","institution_ids":["https://openalex.org/I5023651"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I5023651"],"apc_list":{"value":5400,"currency":"USD","value_usd":5400},"apc_paid":null,"fwci":11.381,"has_fulltext":false,"cited_by_count":128,"citation_normalized_percentile":{"value":0.98774049,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"496","issue":"3","first_page":"687","last_page":"693"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10665","display_name":"Cystic Fibrosis Research Advances","score":0.4652999937534332,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10665","display_name":"Cystic Fibrosis Research Advances","score":0.4652999937534332,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.38350000977516174,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.11209999769926071,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cystic-fibrosis-transmembrane-conductance-regulator","display_name":"cystic fibrosis transmembrane conductance regulator","score":0.9810000061988831},{"id":"https://openalex.org/keywords/cftr-chloride-channel","display_name":"CFTR chloride channel","score":0.7900000214576721},{"id":"https://openalex.org/keywords/dnds","display_name":"DNDS","score":0.8889999985694885},{"id":"https://openalex.org/keywords/dids","display_name":"DIDS","score":0.9990000128746033},{"id":"https://openalex.org/keywords/channel-block","display_name":"channel block","score":0.6200000047683716},{"id":"https://openalex.org/keywords/anion-binding-site","display_name":"anion binding site","score":0.824999988079071},{"id":"https://openalex.org/keywords/voltage-dependent-inhibition","display_name":"voltage-dependent inhibition","score":0.7620000243186951},{"id":"https://openalex.org/keywords/chloride-permeation","display_name":"chloride permeation","score":0.40799999237060547}],"concepts":[{"id":"https://openalex.org/C2779533339","wikidata":"https://www.wikidata.org/wiki/Q4036893","display_name":"DIDS","level":3,"score":0.8538575172424316},{"id":"https://openalex.org/C2778428886","wikidata":"https://www.wikidata.org/wiki/Q420470","display_name":"Cystic fibrosis transmembrane conductance regulator","level":3,"score":0.8206602334976196},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5993445515632629},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5315135717391968},{"id":"https://openalex.org/C181911157","wikidata":"https://www.wikidata.org/wiki/Q389844","display_name":"Membrane potential","level":2,"score":0.5015647411346436},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.470266193151474},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43147775530815125},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4149620532989502},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.3685796856880188},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28025245666503906},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001224","descriptor_name":"Aspartic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013267","descriptor_name":"Stilbenes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017354","descriptor_name":"Point Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017878","descriptor_name":"4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D019005","descriptor_name":"Cystic Fibrosis Transmembrane Conductance Regulator","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D019005","descriptor_name":"Cystic Fibrosis Transmembrane Conductance Regulator","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D019005","descriptor_name":"Cystic Fibrosis Transmembrane Conductance Regulator","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1113/jphysiol.1996.sp021719","doi":"https://doi.org/10.1113/jphysiol.1996.sp021719","is_oa":false,"landing_page_url":"https://doi.org/10.1113/jphysiol.1996.sp021719","pdf_url":null,"source":{"id":"https://openalex.org/S2090548","display_name":"The Journal of Physiology","issn_l":"0022-3751","issn":["0022-3751","1469-7793"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Physiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1160856","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1160856","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Physiol","raw_type":"Text"},{"id":"pmid:8930836","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8930836","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of physiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1160856","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1160856","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Physiol","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W344909913","https://openalex.org/W1856243426","https://openalex.org/W1972554093","https://openalex.org/W2008844827","https://openalex.org/W2015655328","https://openalex.org/W2015759706","https://openalex.org/W2019773946","https://openalex.org/W2028982633","https://openalex.org/W2036136248","https://openalex.org/W2065039955","https://openalex.org/W2076091117","https://openalex.org/W2100432609","https://openalex.org/W2108425243","https://openalex.org/W2399022058","https://openalex.org/W2402530611","https://openalex.org/W2417371011"],"related_works":["https://openalex.org/W1819465909","https://openalex.org/W2098008929","https://openalex.org/W2108425243","https://openalex.org/W7095459385","https://openalex.org/W19134810","https://openalex.org/W2075618475","https://openalex.org/W2062064880","https://openalex.org/W7096793199","https://openalex.org/W2005105608","https://openalex.org/W7097635214"],"abstract_inverted_index":{"1.":[0],"The":[1],"disulphonic":[2,104],"stilbenes":[3],"4,4'-dinitrostilbene-2,2'-disulphonic":[4],"acid":[5,9],"(DNDS)":[6],"and":[7,92,130],"4,4'-diisothiocyanostilbene-2,2'-disulphonic":[8],"(DIDS)":[10],"were":[11],"shown":[12],"to":[13,36,47,70,78,99],"cause":[14],"a":[15,64,79,117,124],"voltage-dependent":[16],"inhibition":[17],"of":[18,40,58,63,87,126],"macroscopic":[19],"cystic":[20],"fibrosis":[21],"transmembrane":[22],"conductance":[23],"regulator":[24],"(CFTR)":[25],"Cl-":[26,113,132],"currents":[27],"expressed":[28],"in":[29,73,122,139],"baby":[30],"hamster":[31],"kidney":[32],"cells":[33],"when":[34],"applied":[35],"the":[37,41,55,59,74,85,100,111],"cytoplasmic":[38],"face":[39],"membrane.":[42,60],"These":[43],"compounds":[44],"are":[45],"known":[46],"be":[48,71,137],"relatively":[49,118],"ineffective":[50],"at":[51],"blocking":[52],"CFTR":[53,112],"from":[54],"extracellular":[56],"side":[57],"2.":[61],"Mutation":[62],"positively":[65],"charged":[66,81],"arginine,":[67],"previously":[68],"suggested":[69,109],"located":[72],"channel":[75,114],"pore":[76],"(R347),":[77],"negatively":[80],"aspartate":[82],"significantly":[83],"reduced":[84],"affinity":[86],"block":[88,131],"by":[89],"both":[90],"DNDS":[91],"DIDS,":[93],"suggesting":[94],"that":[95,110],"this":[96,143],"residue":[97],"contributes":[98],"binding":[101,141],"site":[102],"for":[103],"stilbenes.":[105],"3.":[106],"It":[107],"is":[108],"may":[115,136],"contain":[116],"large":[119,127],"inner":[120],"vestibule":[121],"which":[123],"number":[125],"anions":[128],"bind":[129],"permeation.":[133],"Arginine":[134],"347":[135],"involved":[138],"anion":[140],"within":[142],"region.":[144]},"counts_by_year":[{"year":2024,"cited_by_count":3},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}