{"id":"https://openalex.org/W1991134701","doi":"https://doi.org/10.1016/0167-4838(91)90523-3","title":"Purification and characterization of recombinant mouse thymidylate synthase","display_name":"Purification and characterization of recombinant mouse thymidylate synthase","publication_year":1991,"publication_date":"1991-03-01","ids":{"openalex":"https://openalex.org/W1991134701","doi":"https://doi.org/10.1016/0167-4838(91)90523-3","mag":"1991134701","pmid":"https://pubmed.ncbi.nlm.nih.gov/2009293"},"language":"en","primary_location":{"id":"doi:10.1016/0167-4838(91)90523-3","doi":"https://doi.org/10.1016/0167-4838(91)90523-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0167-4838(91)90523-3","pdf_url":null,"source":{"id":"https://openalex.org/S4394735818","display_name":"Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology","issn_l":"0167-4838","issn":["0167-4838","1879-2588"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101758341","display_name":"Haichao Zhang","orcid":"https://orcid.org/0000-0003-0154-2947","observed_orcids":["https://orcid.org/0000-0003-0154-2947"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haichao Zhang","raw_affiliation_strings":["Department of Biochemistry, Ohio State University, Columbus"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Ohio State University, Columbus","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109981660","display_name":"Ralph J. Cisneros","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ralph J. Cisneros","raw_affiliation_strings":["Department of Chemistry, University of South Carolina, Columbia, SC U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of South Carolina, Columbia, SC U.S.A","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112904352","display_name":"Wanleng Deng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wanleng Deng","raw_affiliation_strings":["Department of Chemistry, University of South Carolina, Columbia, SC U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of South Carolina, Columbia, SC U.S.A","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061698057","display_name":"James W. Zapf","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James W. Zapf","raw_affiliation_strings":["Department of Chemistry, University of South Carolina, Columbia, SC U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of South Carolina, Columbia, SC U.S.A","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110529913","display_name":"Lee F. Johnson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lee F. Johnson","raw_affiliation_strings":["Department of Biochemistry and Molecular Genetics, Ohio State University, Columbus, OH, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Genetics, Ohio State University, Columbus, OH, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000954339","display_name":"R. Bruce Dunlap","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"R.Bruce Dunlap","raw_affiliation_strings":["Department of Chemistry, University of South Carolina, Columbia, SC U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of South Carolina, Columbia, SC U.S.A","institution_ids":["https://openalex.org/I155781252"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5000954339"],"corresponding_institution_ids":["https://openalex.org/I155781252"],"apc_list":null,"apc_paid":null,"fwci":0.3964,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.54631086,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"1077","issue":"1","first_page":"35","last_page":"46"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10499","display_name":"Folate and B Vitamins Research","score":0.005499999970197678,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thymidylate-synthase","display_name":"thymidylate synthase","score":0.996999979019165},{"id":"https://openalex.org/keywords/recombinant-enzyme-purification","display_name":"recombinant enzyme purification","score":0.8209999799728394},{"id":"https://openalex.org/keywords/enzyme-thermal-inactivation","display_name":"enzyme thermal inactivation","score":0.5410000085830688},{"id":"https://openalex.org/keywords/ph-effect-on-enzyme-activity","display_name":"pH-dependent enzyme activity","score":0.7770000100135803},{"id":"https://openalex.org/keywords/5-10-methylenetetrahydrofolate","display_name":"5,10-methylenetetrahydrofolate","score":0.9750000238418579},{"id":"https://openalex.org/keywords/ternary-complex","display_name":"ternary complex","score":0.7450000047683716},{"id":"https://openalex.org/keywords/nucleotide-binding","display_name":"nucleotide binding","score":0.7139999866485596},{"id":"https://openalex.org/keywords/19f-nmr","display_name":"19F NMR","score":0.7200000286102295},{"id":"https://openalex.org/keywords/glycerol-stabilization","display_name":"glycerol stabilization","score":0.8159999847412109},{"id":"https://openalex.org/keywords/enzyme-storage-stability","display_name":"enzyme storage stability","score":0.3840000033378601},{"id":"https://openalex.org/keywords/mouse-enzyme","display_name":"mouse enzyme","score":0.3160000145435333}],"concepts":[{"id":"https://openalex.org/C86756495","wikidata":"https://www.wikidata.org/wiki/Q7702931","display_name":"Ternary complex","level":3,"score":0.8192601799964905},{"id":"https://openalex.org/C123321153","wikidata":"https://www.wikidata.org/wiki/Q22676802","display_name":"Thymidylate synthase","level":4,"score":0.7971834540367126},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.7388873100280762},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6898018717765808},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.6129499077796936},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5815711617469788},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.5715484619140625},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5353216528892517},{"id":"https://openalex.org/C64452783","wikidata":"https://www.wikidata.org/wiki/Q1524945","display_name":"Ternary operation","level":2,"score":0.5253279209136963},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4673008918762207},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.4217022955417633},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3949882984161377},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.33721739053726196},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2863888740539551},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.23488053679466248},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1333509385585785},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07381060719490051},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C2780456651","wikidata":"https://www.wikidata.org/wiki/Q238512","display_name":"Fluorouracil","level":3,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C2776694085","wikidata":"https://www.wikidata.org/wiki/Q974135","display_name":"Chemotherapy","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013940","descriptor_name":"Thymidylate Synthase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013940","descriptor_name":"Thymidylate Synthase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D013940","descriptor_name":"Thymidylate Synthase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0167-4838(91)90523-3","doi":"https://doi.org/10.1016/0167-4838(91)90523-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0167-4838(91)90523-3","pdf_url":null,"source":{"id":"https://openalex.org/S4394735818","display_name":"Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology","issn_l":"0167-4838","issn":["0167-4838","1879-2588"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology","raw_type":"journal-article"},{"id":"pmid:2009293","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2009293","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimica et biophysica acta","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2714647962","display_name":null,"funder_award_id":"CA16058","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G4531782395","display_name":null,"funder_award_id":"CA 15645","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G5124175904","display_name":null,"funder_award_id":"GM-29356","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6485749151","display_name":"DTMP SYNTHASE:  NMR AND MECHANISTIC STUDIES","funder_award_id":"5r01ca015645-16","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6690408620","display_name":"THYMIDYLATE SYNTHASE GENE EXPRESSION","funder_award_id":"5r01gm029356-18","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000887552","display_name":"Cancer Center Support Grant","funder_award_id":"P30CA016058","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320309374","display_name":"Ohio State University","ror":"https://ror.org/00rs6vg23"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W116167402","https://openalex.org/W1159417576","https://openalex.org/W1500523379","https://openalex.org/W1508681581","https://openalex.org/W1524857002","https://openalex.org/W1526727908","https://openalex.org/W1538066396","https://openalex.org/W1551398481","https://openalex.org/W1577697848","https://openalex.org/W1670087559","https://openalex.org/W1971912651","https://openalex.org/W1975304761","https://openalex.org/W1981301625","https://openalex.org/W1984220084","https://openalex.org/W1990138449","https://openalex.org/W1999659254","https://openalex.org/W2003559225","https://openalex.org/W2008887856","https://openalex.org/W2015701131","https://openalex.org/W2021336036","https://openalex.org/W2022585357","https://openalex.org/W2024715692","https://openalex.org/W2032766238","https://openalex.org/W2043172346","https://openalex.org/W2045175745","https://openalex.org/W2051760741","https://openalex.org/W2062296859","https://openalex.org/W2062579188","https://openalex.org/W2063893188","https://openalex.org/W2076160862","https://openalex.org/W2077622701","https://openalex.org/W2083295966","https://openalex.org/W2083817429","https://openalex.org/W2112083550","https://openalex.org/W2114659793","https://openalex.org/W2128635872","https://openalex.org/W2200991233","https://openalex.org/W2755574957"],"related_works":["https://openalex.org/W1966769779","https://openalex.org/W1510646666","https://openalex.org/W2030829521","https://openalex.org/W1997654720","https://openalex.org/W2051285344","https://openalex.org/W2043172346","https://openalex.org/W1594948172","https://openalex.org/W1484217798","https://openalex.org/W2046900458","https://openalex.org/W1987550267"],"abstract_inverted_index":null,"counts_by_year":[{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
