{"id":"https://openalex.org/W1990941929","doi":"https://doi.org/10.1021/jo015890v","title":"Biosynthesis of Terpenoids: Efficient Multistep Biotransformation Procedures Affording Isotope-Labeled 2C-Methyl- d -erythritol 4-Phosphate Using Recombinant 2C-Methyl- d -erythritol 4-Phosphate Synthase","display_name":"Biosynthesis of Terpenoids: Efficient Multistep Biotransformation Procedures Affording Isotope-Labeled 2C-Methyl- d -erythritol 4-Phosphate Using Recombinant 2C-Methyl- d -erythritol 4-Phosphate Synthase","publication_year":2001,"publication_date":"2001-10-13","ids":{"openalex":"https://openalex.org/W1990941929","doi":"https://doi.org/10.1021/jo015890v","mag":"1990941929","pmid":"https://pubmed.ncbi.nlm.nih.gov/11701035"},"language":"en","primary_location":{"id":"doi:10.1021/jo015890v","doi":"https://doi.org/10.1021/jo015890v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jo015890v","pdf_url":null,"source":{"id":"https://openalex.org/S205050996","display_name":"The Journal of Organic Chemistry","issn_l":"0022-3263","issn":["0022-3263","1520-6904"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Organic Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103442785","display_name":"Stefan Hecht","orcid":"https://orcid.org/0000-0002-6124-0222","observed_orcids":["https://orcid.org/0000-0002-6124-0222"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stefan Hecht","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056674108","display_name":"Juraithip Wungsintaweekul","orcid":"https://orcid.org/0000-0002-4711-3606","observed_orcids":["https://orcid.org/0000-0002-4711-3606"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Juraithip Wungsintaweekul","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057528779","display_name":"Felix Rohdich","orcid":"https://orcid.org/0009-0002-9323-4254","observed_orcids":["https://orcid.org/0009-0002-9323-4254"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Felix Rohdich","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013129151","display_name":"Klaus Kis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Klaus Kis","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031881809","display_name":"Tanja Radykewicz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Tanja Radykewicz","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070284202","display_name":"Christoph A. Schuhr","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Christoph A. Schuhr","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086504004","display_name":"Wolfgang Eisenreich","orcid":"https://orcid.org/0000-0002-9832-8279","observed_orcids":["https://orcid.org/0000-0002-9832-8279"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Wolfgang Eisenreich","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079134590","display_name":"Gerald Richter","orcid":"https://orcid.org/0000-0002-2870-1568","observed_orcids":["https://orcid.org/0000-0002-2870-1568"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Gerald Richter","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5068798153","display_name":"Adelbert Bacher","orcid":"https://orcid.org/0000-0001-5174-4139","observed_orcids":["https://orcid.org/0000-0001-5174-4139"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Adelbert Bacher","raw_affiliation_strings":["Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Organische Chemie und Biochemie, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstrasse 4, D-85747 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I62916508"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.8657,"has_fulltext":false,"cited_by_count":33,"citation_normalized_percentile":{"value":0.67981095,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"66","issue":"23","first_page":"7770","last_page":"7775"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/terpenoid-biosynthesis","display_name":"terpenoid biosynthesis","score":0.925000011920929},{"id":"https://openalex.org/keywords/recombinant-enzyme-production","display_name":"recombinant enzyme expression","score":0.8539999723434448},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8579999804496765},{"id":"https://openalex.org/keywords/isotopic-labeling","display_name":"isotopic labeling","score":0.7440000176429749},{"id":"https://openalex.org/keywords/isotopomers","display_name":"isotopomers","score":0.6990000009536743},{"id":"https://openalex.org/keywords/biotransformation","display_name":"biotransformation","score":0.8330000042915344}],"concepts":[{"id":"https://openalex.org/C2779626320","wikidata":"https://www.wikidata.org/wiki/Q421873","display_name":"Erythritol","level":2,"score":0.9549424648284912},{"id":"https://openalex.org/C27881333","wikidata":"https://www.wikidata.org/wiki/Q864895","display_name":"Biotransformation","level":3,"score":0.7082338333129883},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6690080165863037},{"id":"https://openalex.org/C127124265","wikidata":"https://www.wikidata.org/wiki/Q2100870","display_name":"Isotopomers","level":3,"score":0.5523991584777832},{"id":"https://openalex.org/C2777132085","wikidata":"https://www.wikidata.org/wiki/Q46220103","display_name":"Phosphate","level":2,"score":0.5121650695800781},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.4872835874557495},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.4808140993118286},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4699195623397827},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4599074721336365},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.44879451394081116},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4392399191856384},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4301377832889557},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3308253884315491},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.26484552025794983},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08880147337913513},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001711","descriptor_name":"Biotransformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002250","descriptor_name":"Carbon Radioisotopes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004896","descriptor_name":"Erythritol","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D004896","descriptor_name":"Erythritol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004896","descriptor_name":"Erythritol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013403","descriptor_name":"Sugar Phosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013403","descriptor_name":"Sugar Phosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013729","descriptor_name":"Terpenes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013729","descriptor_name":"Terpenes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019747","descriptor_name":"Aldose-Ketose Isomerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019747","descriptor_name":"Aldose-Ketose Isomerases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/jo015890v","doi":"https://doi.org/10.1021/jo015890v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jo015890v","pdf_url":null,"source":{"id":"https://openalex.org/S205050996","display_name":"The Journal of Organic Chemistry","issn_l":"0022-3263","issn":["0022-3263","1520-6904"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Organic Chemistry","raw_type":"journal-article"},{"id":"pmid:11701035","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11701035","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of organic chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1969144272","https://openalex.org/W1973570145","https://openalex.org/W1975639729","https://openalex.org/W1999097194","https://openalex.org/W2036479712","https://openalex.org/W2040789128","https://openalex.org/W2041792339","https://openalex.org/W2081027578","https://openalex.org/W2089876480","https://openalex.org/W2090087823","https://openalex.org/W2138270253","https://openalex.org/W2164267446","https://openalex.org/W2172191471"],"related_works":["https://openalex.org/W1934023196","https://openalex.org/W2043419252","https://openalex.org/W2035415464","https://openalex.org/W2169545742","https://openalex.org/W1995004254","https://openalex.org/W2167642221","https://openalex.org/W2953180775","https://openalex.org/W2461918568","https://openalex.org/W2122679530","https://openalex.org/W2081290361"],"abstract_inverted_index":{"This":[0],"paper":[1],"describes":[2],"the":[3,7,34,73],"recombinant":[4],"expression":[5],"of":[6,10,36,56],"ispC":[8],"gene":[9],"Escherichia":[11],"coli":[12],"specifying":[13],"2C-methyl-D-erythritol":[14,38,57],"4-phosphate":[15,39,58],"synthase":[16],"in":[17],"a":[18],"modified":[19],"form":[20],"that":[21],"can":[22],"be":[23],"purified":[24],"efficiently":[25],"by":[26],"metal-chelating":[27],"chromatography.":[28],"The":[29,48,68],"enzyme":[30],"was":[31],"used":[32],"for":[33],"preparation":[35],"isotope-labeled":[37,41,75],"employing":[40],"glucose":[42],"and":[43],"pyruvate":[44],"as":[45],"starting":[46,76],"materials.":[47],"simple":[49],"one-pot":[50],"methods":[51],"described":[52],"afford":[53],"numerous":[54],"isotopomers":[55],"carrying":[59],"(3)H,":[60],"(13)C,":[61],"or":[62],"(14)C":[63],"from":[64],"commercially":[65],"available":[66],"precursors.":[67],"overall":[69],"yield":[70],"based":[71],"on":[72],"respective":[74],"material":[77],"is":[78],"approximately":[79],"50%.":[80]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}