{"id":"https://openalex.org/W1990667712","doi":"https://doi.org/10.1002/bit.260400313","title":"How to predict product yield in kinetically controlled enzymatic peptide synthesis","display_name":"How to predict product yield in kinetically controlled enzymatic peptide synthesis","publication_year":1992,"publication_date":"1992-07-01","ids":{"openalex":"https://openalex.org/W1990667712","doi":"https://doi.org/10.1002/bit.260400313","mag":"1990667712","pmid":"https://pubmed.ncbi.nlm.nih.gov/18601134"},"language":"en","primary_location":{"id":"doi:10.1002/bit.260400313","doi":"https://doi.org/10.1002/bit.260400313","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260400313","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007552938","display_name":"Mikhail Yu. Gololobov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I187531555","display_name":"Lund University","ror":"https://ror.org/012a77v79","country_code":"SE","type":"education","lineage":["https://openalex.org/I187531555"]},{"id":"https://openalex.org/I4210163576","display_name":"Genetika","ror":"https://ror.org/05hr7qe62","country_code":"RU","type":"facility","lineage":["https://openalex.org/I4210163576"]}],"countries":["RU","SE"],"is_corresponding":true,"raw_author_name":"Mikhail Yu. Gololobov","raw_affiliation_strings":["Institute for Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia","Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S\u2010221 00 Lund, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia","institution_ids":["https://openalex.org/I4210163576"]},{"raw_affiliation_string":"Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S\u2010221 00 Lund, Sweden","institution_ids":["https://openalex.org/I187531555"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Elena V. Kozlova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Elena V. Kozlova","raw_affiliation_strings":["Belozersky Institute of Physio-Chemical Biology, Moscow University, Moscow 119899, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Belozersky Institute of Physio-Chemical Biology, Moscow University, Moscow 119899, Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076111969","display_name":"Ivan L. Borisov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Ivan L. Borisov","raw_affiliation_strings":["Belozersky Institute of Physio-Chemical Biology, Moscow University, Moscow 119899, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Belozersky Institute of Physio-Chemical Biology, Moscow University, Moscow 119899, Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065816810","display_name":"Ute Schellenberger","orcid":"https://orcid.org/0000-0002-4809-9383","observed_orcids":["https://orcid.org/0000-0002-4809-9383"]},"institutions":[{"id":"https://openalex.org/I926574661","display_name":"Leipzig University","ror":"https://ror.org/03s7gtk40","country_code":"DE","type":"education","lineage":["https://openalex.org/I926574661"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ute Schellenberger","raw_affiliation_strings":["Leipzig University, Bioscience Division, Department of Biochemistry, Talstrasse 33, Leipzig D-0-7010, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Leipzig University, Bioscience Division, Department of Biochemistry, Talstrasse 33, Leipzig D-0-7010, Germany","institution_ids":["https://openalex.org/I926574661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018783910","display_name":"Volker Schellenberger","orcid":"https://orcid.org/0000-0002-3491-8229","observed_orcids":["https://orcid.org/0000-0002-3491-8229"]},"institutions":[{"id":"https://openalex.org/I926574661","display_name":"Leipzig University","ror":"https://ror.org/03s7gtk40","country_code":"DE","type":"education","lineage":["https://openalex.org/I926574661"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Volker Schellenberger","raw_affiliation_strings":["Leipzig University, Bioscience Division, Department of Biochemistry, Talstrasse 33, Leipzig D-0-7010, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Leipzig University, Bioscience Division, Department of Biochemistry, Talstrasse 33, Leipzig D-0-7010, Germany","institution_ids":["https://openalex.org/I926574661"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047363996","display_name":"Hans\u2010Dieter Jakubke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I926574661","display_name":"Leipzig University","ror":"https://ror.org/03s7gtk40","country_code":"DE","type":"education","lineage":["https://openalex.org/I926574661"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Hans\u2010Dieter Jakubke","raw_affiliation_strings":["Leipzig University, Bioscience Division, Department of Biochemistry, Talstrasse 33, Leipzig D-0-7010, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Leipzig University, Bioscience Division, Department of Biochemistry, Talstrasse 33, Leipzig D-0-7010, Germany","institution_ids":["https://openalex.org/I926574661"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5007552938"],"corresponding_institution_ids":["https://openalex.org/I187531555","https://openalex.org/I4210163576"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":0.6554,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.64204525,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"40","issue":"3","first_page":"432","last_page":"436"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.7958999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.7958999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.17679999768733978,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzymatic-peptide-synthesis","display_name":"enzymatic peptide synthesis","score":0.9290000200271606},{"id":"https://openalex.org/keywords/product-yield-prediction","display_name":"product yield prediction","score":0.8980000019073486},{"id":"https://openalex.org/keywords/papain","display_name":"papain","score":0.8759999871253967},{"id":"https://openalex.org/keywords/peptide-bond-cleavage","display_name":"peptide bond cleavage","score":0.3889999985694885},{"id":"https://openalex.org/keywords/methionine-containing-peptides","display_name":"methionine-containing peptides","score":0.4350000023841858}],"concepts":[{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.8036571145057678},{"id":"https://openalex.org/C2779832904","wikidata":"https://www.wikidata.org/wiki/Q410420","display_name":"Papain","level":3,"score":0.7411587834358215},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.7247987389564514},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5831159353256226},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5594480037689209},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.5252479910850525},{"id":"https://openalex.org/C2779809887","wikidata":"https://www.wikidata.org/wiki/Q14864278","display_name":"Peptide synthesis","level":3,"score":0.5213005542755127},{"id":"https://openalex.org/C2776174256","wikidata":"https://www.wikidata.org/wiki/Q830842","display_name":"BETA (programming language)","level":2,"score":0.44352298974990845},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3334088921546936},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28360581398010254},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.14657673239707947},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.08817106485366821},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.07383391261100769},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.260400313","doi":"https://doi.org/10.1002/bit.260400313","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260400313","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:18601134","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18601134","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1964262399","https://openalex.org/W1986094359","https://openalex.org/W2002150433","https://openalex.org/W2016661872","https://openalex.org/W2032920834","https://openalex.org/W2068943002","https://openalex.org/W2113946213","https://openalex.org/W2156065136","https://openalex.org/W2397292010","https://openalex.org/W2409104213"],"related_works":["https://openalex.org/W1969719875","https://openalex.org/W1499356557","https://openalex.org/W4299655557","https://openalex.org/W2082051906","https://openalex.org/W2050053212","https://openalex.org/W2043820238","https://openalex.org/W4255147393","https://openalex.org/W2290006393","https://openalex.org/W277652106","https://openalex.org/W2156065136"],"abstract_inverted_index":{"The":[0,39],"published":[1],"theory":[2,45],"of":[3,17,23,33],"kinetically":[4],"controlled":[5],"enzymatic":[6],"peptide":[7,19],"synthesis":[8,32],"needed":[9],"experimental":[10,40],"verification.":[11],"We":[12],"carried":[13],"out":[14],"the":[15,18,24,48],"measurement":[16],"yield":[20],"and":[21,28,37],"estimation":[22],"key":[25],"parameters":[26],"alpha":[27],"beta":[29],"for":[30],"papain-catalyzed":[31],"Mal-L-Phe-L-Ala-LLeuNH(2)":[34],"from":[35],"Mal-L-Phe-L-AlaOMe":[36],"L-LeuNH(2).":[38],"results":[41],"demonstrate":[42],"that":[43],"this":[44],"adequately":[46],"describes":[47],"real":[49],"process.":[50]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-07T09:51:55.459714","created_date":"2016-06-24T00:00:00"}