{"id":"https://openalex.org/W1990215410","doi":"https://doi.org/10.1021/bi061696x","title":"Sodium-Independent Low-Affinity d -Glucose Transport by Human Sodium/ d -Glucose Cotransporter 1: Critical Role of Tryptophan 561","display_name":"Sodium-Independent Low-Affinity d -Glucose Transport by Human Sodium/ d -Glucose Cotransporter 1: Critical Role of Tryptophan 561","publication_year":2007,"publication_date":"2007-02-09","ids":{"openalex":"https://openalex.org/W1990215410","doi":"https://doi.org/10.1021/bi061696x","mag":"1990215410","pmid":"https://pubmed.ncbi.nlm.nih.gov/17288452"},"language":"en","primary_location":{"id":"doi:10.1021/bi061696x","doi":"https://doi.org/10.1021/bi061696x","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi061696x","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068501336","display_name":"Azad Kumar","orcid":"https://orcid.org/0000-0001-5162-7768","observed_orcids":["https://orcid.org/0000-0001-5162-7768"]},"institutions":[{"id":"https://openalex.org/I4210130622","display_name":"Max Planck Institute of Molecular Physiology","ror":"https://ror.org/03vpj4s62","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210130622"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Azad Kumar","raw_affiliation_strings":["Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany","institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028744978","display_name":"Navneet K. Tyagi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210130622","display_name":"Max Planck Institute of Molecular Physiology","ror":"https://ror.org/03vpj4s62","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210130622"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Navneet K. Tyagi","raw_affiliation_strings":["Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany","institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067101223","display_name":"Pankaj Goyal","orcid":"https://orcid.org/0000-0002-9916-654X","observed_orcids":["https://orcid.org/0000-0002-9916-654X"]},"institutions":[{"id":"https://openalex.org/I4210130622","display_name":"Max Planck Institute of Molecular Physiology","ror":"https://ror.org/03vpj4s62","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210130622"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Pankaj Goyal","raw_affiliation_strings":["Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany","institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032528582","display_name":"Dharmendra B Pandey","orcid":"https://orcid.org/0000-0002-3172-5923","observed_orcids":["https://orcid.org/0000-0002-3172-5923"]},"institutions":[{"id":"https://openalex.org/I4210130622","display_name":"Max Planck Institute of Molecular Physiology","ror":"https://ror.org/03vpj4s62","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210130622"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Dharmendra Pandey","raw_affiliation_strings":["Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany","institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015728629","display_name":"Wolfgang Siess","orcid":"https://orcid.org/0000-0003-0698-6416","observed_orcids":["https://orcid.org/0000-0003-0698-6416"]},"institutions":[{"id":"https://openalex.org/I4210130622","display_name":"Max Planck Institute of Molecular Physiology","ror":"https://ror.org/03vpj4s62","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210130622"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Wolfgang Siess","raw_affiliation_strings":["Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany","institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5017423382","display_name":"Rolf K. H. Kinne","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210130622","display_name":"Max Planck Institute of Molecular Physiology","ror":"https://ror.org/03vpj4s62","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210130622"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Rolf K. H. Kinne","raw_affiliation_strings":["Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute of Molecular Physiology, Otto-Hahn Strasse 11, 44227 Dortmund, Germany, and Institute for Prevention of Cardiovascular Disease, Ludwig Maxmilian University, Pettenkoferstrasse 9, 80336 Munich, Germany","institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5017423382"],"corresponding_institution_ids":["https://openalex.org/I4210130622","https://openalex.org/I8204097"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.6528,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.6183253,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"46","issue":"10","first_page":"2758","last_page":"2766"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.7250999808311462,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.7250999808311462,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.07680000364780426,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.04019999876618385,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sglt1","display_name":"SGLT1","score":0.8510000109672546},{"id":"https://openalex.org/keywords/methyl-alpha-d-glucopyranoside","display_name":"methyl \u03b1-D-glucopyranoside","score":0.8100000023841858},{"id":"https://openalex.org/keywords/sugar-binding-site","display_name":"sugar-binding site","score":0.6690000295639038},{"id":"https://openalex.org/keywords/protein-conformation","display_name":"protein conformation","score":0.4650000035762787},{"id":"https://openalex.org/keywords/tryptophan-fluorescence","display_name":"tryptophan fluorescence","score":0.8240000009536743}],"concepts":[{"id":"https://openalex.org/C2778117581","wikidata":"https://www.wikidata.org/wiki/Q413105","display_name":"Phlorizin","level":4,"score":0.9378082752227783},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8018412590026855},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.7389603853225708},{"id":"https://openalex.org/C188053792","wikidata":"https://www.wikidata.org/wiki/Q3640664","display_name":"Cotransporter","level":3,"score":0.7378202676773071},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5757513046264648},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.5451383590698242},{"id":"https://openalex.org/C161573976","wikidata":"https://www.wikidata.org/wiki/Q420277","display_name":"Glucose transporter","level":3,"score":0.5009477138519287},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3317570984363556},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.19662559032440186},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12771177291870117},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.12751427292823792},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.10511225461959839},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001692","descriptor_name":"Biological Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010695","descriptor_name":"Phlorhizin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011510","descriptor_name":"Proteolipids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012964","descriptor_name":"Sodium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051269","descriptor_name":"Sodium-Glucose Transporter 1","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051269","descriptor_name":"Sodium-Glucose Transporter 1","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1021/bi061696x","doi":"https://doi.org/10.1021/bi061696x","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi061696x","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:17288452","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17288452","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:edoc.mpg.de:307713","doi":null,"is_oa":false,"landing_page_url":"http://edoc.mpg.de/307713","pdf_url":null,"source":{"id":"https://openalex.org/S4406922265","display_name":"Max Planck Institute for Plasma Physics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry, v.46, 2758-2766 (2007)","raw_type":"Article"},{"id":"pmh:oai:pure.mpg.de:item_1812736","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/11858/00-001M-0000-0014-06F5-8","pdf_url":null,"source":{"id":"https://openalex.org/S4306400654","display_name":"MPG.PuRe (Max Planck Society)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I149899117","host_organization_name":"Max Planck Society","host_organization_lineage":["https://openalex.org/I149899117"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1506378995","https://openalex.org/W1968454815","https://openalex.org/W1976995597","https://openalex.org/W2006903256","https://openalex.org/W2020817480","https://openalex.org/W2038492881","https://openalex.org/W2046332315","https://openalex.org/W2047614216","https://openalex.org/W2081926853","https://openalex.org/W2313071154"],"related_works":["https://openalex.org/W2024767950","https://openalex.org/W2052691710","https://openalex.org/W7064915150","https://openalex.org/W2127718379","https://openalex.org/W2046634986","https://openalex.org/W2111321455","https://openalex.org/W2149185387","https://openalex.org/W3162683170","https://openalex.org/W1512501096","https://openalex.org/W1549818147"],"abstract_inverted_index":{"Although":[0],"there":[1],"is":[2,225],"no":[3],"evidence":[4],"of":[5,51,65,88,95,121,126,136,144,161,195,206,217,260,272],"significant":[6,73,158],"Na-independent":[7],"glucose":[8],"flux":[9],"in":[10,55,62,92,118,138,179,185,203,227,240,256,283],"tissues":[11],"naturally":[12],"expressing":[13],"SGLT1,":[14],"previous":[15],"kinetic":[16,46],"and":[17,31,48,59,208,236],"biophysical":[18],"studies":[19,61,70],"suggest":[20],"that":[21,117,254],"sodium/d-glucose":[22],"cotransporter":[23],"1":[24],"(hSGLT1)":[25],"can":[26],"facilitate":[27],"sodium-independent":[28,74,263,273],"d-glucose":[29,148,162],"transport":[30,58,69],"may":[32],"contain":[33],"more":[34],"than":[35,116],"one":[36],"sugar":[37],"binding":[38,264],"site.":[39],"In":[40,67,123],"this":[41,180],"work,":[42],"we":[43,182],"analyze":[44],"the":[45,63,68,89,93,119,124,132,141,176,196,204,215,284],"properties":[47],"conformational":[49],"states":[50],"isolated":[52],"hSGLT1":[53,137,257,276],"reconstituted":[54],"liposomes":[56],"by":[57,287],"fluorescence":[60,135,210],"absence":[64,125,205],"sodium.":[66,122],"with":[71,192],"hSGLT1,":[72],"phlorizin":[75,164],"inhibitable":[76],"alpha-methyl":[77],"d-glucopyranoside":[78],"(alpha-MDG)":[79],"uptake":[80,90],"was":[81,105],"observed":[82,91],"which":[83,186],"amounted":[84],"to":[85,279],"approximately":[86,113,149],"20%":[87],"presence":[94,120,216],"a":[96,111,261],"sodium":[97,207],"gradient.":[98],"The":[99,270],"apparent":[100],"affinity":[101],"constant":[102],"for":[103,268],"alpha-MDG":[104,146],"thereby":[106],"3.4":[107],"+/-":[108],"0.5":[109],"mM,":[110],"value":[112],"10-fold":[114],"higher":[115],"sodium,":[127],"various":[128],"sugars":[129],"significantly":[130,198],"decreased":[131],"intrinsic":[133],"Trp":[134,218,223],"proteoliposomes":[139],"exhibiting":[140],"following":[142],"sequence":[143],"affinities:":[145],">":[147,151,153,155],"d-galactose":[150],"6-deoxy-d-glucose":[152],"2-deoxy-d-glucose":[154],"d-allose.":[156],"Furthermore,":[157],"protection":[159],"effects":[160],"or":[163,169],"against":[165],"potassium":[166],"iodide,":[167],"acrylamide,":[168],"trichloroethanol":[170],"quenching":[171],"were":[172,189],"observed.":[173],"To":[174],"locate":[175],"Trps":[177,188],"involved":[178],"reaction,":[181],"generated":[183],"mutants":[184],"all":[187,228,237,245],"sequentially":[190],"substituted":[191],"Phe.":[193],"None":[194],"replacements":[197],"affected":[199],"sodium-dependent":[200],"uptake.":[201],"Uptake":[202],"typical":[209],"changes":[211],"depended,":[212],"however,":[213,278],"on":[214],"at":[219],"position":[220],"561.":[221],"This":[222],"residue":[224],"conserved":[226],"known":[229],"SGLT1":[230],"forms":[231,258],"(except":[232,242],"Vibrio":[233],"parahaemolyticus":[234],"SGLT)":[235],"SGLT":[238],"isoforms":[239],"humans":[241],"hSGLT3).":[243],"If":[244],"these":[246],"data":[247],"are":[248],"taken":[249],"into":[250],"consideration,":[251],"it":[252],"seems":[253],"Trp-561":[255],"part":[259],"low-affinity":[262],"and/or":[265],"translocation":[266,274],"site":[267],"d-glucose.":[269],"rate":[271],"via":[275],"seems,":[277],"be":[280],"tightly":[281],"regulated":[282],"intact":[285],"cell":[286],"yet":[288],"unknown":[289],"factors.":[290]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}