{"id":"https://openalex.org/W1989811599","doi":"https://doi.org/10.1016/s0022-1759(03)00227-8","title":"The humoral response to in vitro generated parasite antigens is enhanced by the removal of a defined media component prior to immunization","display_name":"The humoral response to in vitro generated parasite antigens is enhanced by the removal of a defined media component prior to immunization","publication_year":2003,"publication_date":"2003-06-09","ids":{"openalex":"https://openalex.org/W1989811599","doi":"https://doi.org/10.1016/s0022-1759(03)00227-8","mag":"1989811599","pmid":"https://pubmed.ncbi.nlm.nih.gov/12957397"},"language":"en","primary_location":{"id":"doi:10.1016/s0022-1759(03)00227-8","doi":"https://doi.org/10.1016/s0022-1759(03)00227-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0022-1759(03)00227-8","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013009460","display_name":"Christopher G. Earnhart","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16285277","display_name":"William & Mary","ror":"https://ror.org/03hsf0573","country_code":"US","type":"education","lineage":["https://openalex.org/I16285277"]},{"id":"https://openalex.org/I57851904","display_name":"Virginia Institute of Marine Science","ror":"https://ror.org/04eeqc889","country_code":"US","type":"education","lineage":["https://openalex.org/I57851904"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher G. Earnhart","raw_affiliation_strings":["Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, P.O. Box 1346, Gloucester Point, VA 23062, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, P.O. Box 1346, Gloucester Point, VA 23062, USA","institution_ids":["https://openalex.org/I16285277","https://openalex.org/I57851904"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110306886","display_name":"Stephen L. Kaattari","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16285277","display_name":"William & Mary","ror":"https://ror.org/03hsf0573","country_code":"US","type":"education","lineage":["https://openalex.org/I16285277"]},{"id":"https://openalex.org/I57851904","display_name":"Virginia Institute of Marine Science","ror":"https://ror.org/04eeqc889","country_code":"US","type":"education","lineage":["https://openalex.org/I57851904"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Stephen L. Kaattari","raw_affiliation_strings":["Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, P.O. Box 1346, Gloucester Point, VA 23062, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, P.O. Box 1346, Gloucester Point, VA 23062, USA","institution_ids":["https://openalex.org/I16285277","https://openalex.org/I57851904"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5110306886"],"corresponding_institution_ids":["https://openalex.org/I16285277","https://openalex.org/I57851904"],"apc_list":{"value":2970,"currency":"USD","value_usd":2970},"apc_paid":null,"fwci":0.4329,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.59892892,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"278","issue":"1-2","first_page":"67","last_page":"78"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.28049999475479126,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.28049999475479126,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14072","display_name":"Immunotoxicology and immune responses","score":0.14419999718666077,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11088","display_name":"Marine Bivalve and Aquaculture Studies","score":0.12030000239610672,"subfield":{"id":"https://openalex.org/subfields/2306","display_name":"Global and Planetary Change"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/perkinsus-marinus","display_name":"Perkinsus marinus","score":0.9900000095367432},{"id":"https://openalex.org/keywords/oyster-parasites","display_name":"oyster parasites","score":0.628000020980835},{"id":"https://openalex.org/keywords/extracellular-products","display_name":"extracellular products","score":0.828000009059906},{"id":"https://openalex.org/keywords/immunosuppression","display_name":"immunosuppression","score":0.8349999785423279},{"id":"https://openalex.org/keywords/poloxamer-188","display_name":"poloxamer 188","score":0.8830000162124634},{"id":"https://openalex.org/keywords/surfactants","display_name":"surfactants","score":0.656000018119812},{"id":"https://openalex.org/keywords/culture-medium-components","display_name":"culture medium components","score":0.4560000002384186},{"id":"https://openalex.org/keywords/antibody-response","display_name":"antibody response","score":0.5609999895095825},{"id":"https://openalex.org/keywords/epitope-recognition","display_name":"epitope recognition","score":0.6650000214576721},{"id":"https://openalex.org/keywords/polyclonal-antisera","display_name":"polyclonal antisera","score":0.7379999756813049},{"id":"https://openalex.org/keywords/immunogenicity","display_name":"immunogenicity","score":0.5929999947547913}],"concepts":[{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.7606875896453857},{"id":"https://openalex.org/C2780868878","wikidata":"https://www.wikidata.org/wiki/Q1660197","display_name":"Immunogenicity","level":3,"score":0.6837033033370972},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6677930355072021},{"id":"https://openalex.org/C100068826","wikidata":"https://www.wikidata.org/wiki/Q485675","display_name":"Hemolymph","level":2,"score":0.589583694934845},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5595613121986389},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.501333475112915},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.4419505298137665},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4259741008281708},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3275466859340668},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15489771962165833}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006458","descriptor_name":"Hemolymph","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D007165","descriptor_name":"Immunosuppression Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008807","descriptor_name":"Mice, Inbred BALB C","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010125","descriptor_name":"Ostreidae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010664","descriptor_name":"Phenylmethylsulfonyl Fluoride","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014611","descriptor_name":"Vaccination","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D020442","descriptor_name":"Poloxamer","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D020442","descriptor_name":"Poloxamer","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0022-1759(03)00227-8","doi":"https://doi.org/10.1016/s0022-1759(03)00227-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0022-1759(03)00227-8","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},{"id":"pmid:12957397","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12957397","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332181","display_name":"National Oceanic and Atmospheric Administration","ror":"https://ror.org/02z5nhe81"},{"id":"https://openalex.org/F4320332282","display_name":"Virginia Sea Grant, Virginia Institute of Marine Science","ror":"https://ror.org/05c6y9623"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":41,"referenced_works":["https://openalex.org/W196010173","https://openalex.org/W1502243809","https://openalex.org/W1521633952","https://openalex.org/W1977255969","https://openalex.org/W1980813530","https://openalex.org/W1983777786","https://openalex.org/W1985989870","https://openalex.org/W1986582364","https://openalex.org/W1993940802","https://openalex.org/W1994544504","https://openalex.org/W2004352247","https://openalex.org/W2015201486","https://openalex.org/W2028291107","https://openalex.org/W2041241685","https://openalex.org/W2056535115","https://openalex.org/W2057990532","https://openalex.org/W2062108399","https://openalex.org/W2068174749","https://openalex.org/W2073685448","https://openalex.org/W2078667033","https://openalex.org/W2078760497","https://openalex.org/W2080159310","https://openalex.org/W2100837269","https://openalex.org/W2113417661","https://openalex.org/W2121561376","https://openalex.org/W2127151076","https://openalex.org/W2140301468","https://openalex.org/W2148962899","https://openalex.org/W2151992793","https://openalex.org/W2157736842","https://openalex.org/W2288217893","https://openalex.org/W2301740330","https://openalex.org/W2410992326","https://openalex.org/W2460746151","https://openalex.org/W2484219288","https://openalex.org/W2602697305","https://openalex.org/W2758388649","https://openalex.org/W2767634887","https://openalex.org/W4285719527","https://openalex.org/W6718661717","https://openalex.org/W6744424096"],"related_works":["https://openalex.org/W2155931741","https://openalex.org/W1966034265","https://openalex.org/W2001482179","https://openalex.org/W2031526624","https://openalex.org/W2470782761","https://openalex.org/W2081288629","https://openalex.org/W2439406204","https://openalex.org/W1967084877","https://openalex.org/W2035591480","https://openalex.org/W2156809280"],"abstract_inverted_index":null,"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}