{"id":"https://openalex.org/W1989358564","doi":"https://doi.org/10.1186/1471-2091-11-40","title":"Novel \u03b2-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification","display_name":"Novel \u03b2-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W1989358564","doi":"https://doi.org/10.1186/1471-2091-11-40","mag":"1989358564","pmid":"https://pubmed.ncbi.nlm.nih.gov/20920218","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2955587"},"language":"en","primary_location":{"id":"doi:10.1186/1471-2091-11-40","doi":"https://doi.org/10.1186/1471-2091-11-40","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2091-11-40","pdf_url":"https://bmcbiochem.biomedcentral.com/counter/pdf/10.1186/1471-2091-11-40","source":{"id":"https://openalex.org/S120371716","display_name":"BMC Biochemistry","issn_l":"1471-2091","issn":["1471-2091"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","poland-70"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://bmcbiochem.biomedcentral.com/counter/pdf/10.1186/1471-2091-11-40","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010991829","display_name":"Wipa Suginta","orcid":"https://orcid.org/0000-0001-5927-7192","observed_orcids":["https://orcid.org/0000-0001-5927-7192"]},"institutions":[{"id":"https://openalex.org/I82475049","display_name":"Suranaree University of Technology","ror":"https://ror.org/05sgb8g78","country_code":"TH","type":"education","lineage":["https://openalex.org/I82475049"]}],"countries":["TH"],"is_corresponding":true,"raw_author_name":"Wipa Suginta","raw_affiliation_strings":["Biochemistry-Electrochemistry Research Unit, School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. wipa@sut.ac.th","Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry-Electrochemistry Research Unit, School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. wipa@sut.ac.th","institution_ids":["https://openalex.org/I82475049"]},{"raw_affiliation_string":"Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand","institution_ids":["https://openalex.org/I82475049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080394234","display_name":"Duangkamon Chuenark","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I82475049","display_name":"Suranaree University of Technology","ror":"https://ror.org/05sgb8g78","country_code":"TH","type":"education","lineage":["https://openalex.org/I82475049"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Duangkamon Chuenark","raw_affiliation_strings":["Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand","Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand","institution_ids":["https://openalex.org/I82475049"]},{"raw_affiliation_string":"Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand","institution_ids":["https://openalex.org/I82475049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024102092","display_name":"Mamiko Mizuhara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mamiko Mizuhara","raw_affiliation_strings":["Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara, 631-8505, Japan","Department of Advanced Bioscience, Kinki University , Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara, 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Department of Advanced Bioscience, Kinki University , Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079809709","display_name":"Tamo Fukamizo","orcid":"https://orcid.org/0000-0002-4713-0246","observed_orcids":["https://orcid.org/0000-0002-4713-0246"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tamo Fukamizo","raw_affiliation_strings":["Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara, 631-8505, Japan","Department of Advanced Bioscience, Kinki University , Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Advanced Bioscience, Kinki University, 3327-204 Nakamachi, Nara, 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Department of Advanced Bioscience, Kinki University , Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5010991829"],"corresponding_institution_ids":["https://openalex.org/I82475049"],"apc_list":{"value":2170,"currency":"USD","value_usd":2170},"apc_paid":{"value":2170,"currency":"USD","value_usd":2170},"fwci":0.8495,"has_fulltext":true,"cited_by_count":58,"citation_normalized_percentile":{"value":0.67634494,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"11","issue":"1","first_page":"40","last_page":"40"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12683","display_name":"Studies on Chitinases and Chitosanases","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12683","display_name":"Studies on Chitinases and Chitosanases","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/n-acetyl-beta-d-glucosaminidase","display_name":"N-acetyl-beta-D-glucosaminidase","score":0.9779999852180481},{"id":"https://openalex.org/keywords/chitin-degradation","display_name":"chitin degradation","score":0.8820000290870667},{"id":"https://openalex.org/keywords/recombinant-enzyme-production","display_name":"recombinant enzyme expression","score":0.6890000104904175},{"id":"https://openalex.org/keywords/chitinase-a","display_name":"chitinase A","score":0.8320000171661377}],"concepts":[{"id":"https://openalex.org/C2780778831","wikidata":"https://www.wikidata.org/wiki/Q424099","display_name":"Chitinase","level":3,"score":0.7551165819168091},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7348944544792175},{"id":"https://openalex.org/C2781275326","wikidata":"https://www.wikidata.org/wiki/Q3761158","display_name":"Vibrio harveyi","level":4,"score":0.7117356061935425},{"id":"https://openalex.org/C2781088250","wikidata":"https://www.wikidata.org/wiki/Q161219","display_name":"Chitin","level":3,"score":0.6285470128059387},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6024448871612549},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.5552300810813904},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5336205959320068},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5022408962249756},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4201987385749817},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38448256254196167},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.37303590774536133},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.2515478730201721},{"id":"https://openalex.org/C2776154503","wikidata":"https://www.wikidata.org/wiki/Q753490","display_name":"Vibrio","level":3,"score":0.251112163066864},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.24558362364768982},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09562933444976807},{"id":"https://openalex.org/C2779732960","wikidata":"https://www.wikidata.org/wiki/Q408510","display_name":"Chitosan","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000118","descriptor_name":"Acetylglucosaminidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000118","descriptor_name":"Acetylglucosaminidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000118","descriptor_name":"Acetylglucosaminidase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002686","descriptor_name":"Chitin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014733","descriptor_name":"Vibrio","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":8,"locations":[{"id":"doi:10.1186/1471-2091-11-40","doi":"https://doi.org/10.1186/1471-2091-11-40","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2091-11-40","pdf_url":"https://bmcbiochem.biomedcentral.com/counter/pdf/10.1186/1471-2091-11-40","source":{"id":"https://openalex.org/S120371716","display_name":"BMC Biochemistry","issn_l":"1471-2091","issn":["1471-2091"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","poland-70"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biochemistry","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:1967202","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2955587","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:20920218","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20920218","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2955587","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2955587/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biochem","raw_type":"Text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.350.9735","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.350.9735","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/4c/ff/BMC_Biochem_2010_Sep_29_11_40.tar.gz","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:8ef1a06a59904d56a902b6c388f0c5c3","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/8ef1a06a59904d56a902b6c388f0c5c3","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Biochemistry, Vol 11, Iss 1, p 40 (2010)","raw_type":"article"},{"id":"doi:10.60692/905zk-azq11","doi":"https://doi.org/10.60692/905zk-azq11","is_oa":true,"landing_page_url":"https://doi.org/10.60692/905zk-azq11","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"doi:10.60692/xt81w-hwq41","doi":"https://doi.org/10.60692/xt81w-hwq41","is_oa":true,"landing_page_url":"https://doi.org/10.60692/xt81w-hwq41","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.1186/1471-2091-11-40","doi":"https://doi.org/10.1186/1471-2091-11-40","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2091-11-40","pdf_url":"https://bmcbiochem.biomedcentral.com/counter/pdf/10.1186/1471-2091-11-40","source":{"id":"https://openalex.org/S120371716","display_name":"BMC Biochemistry","issn_l":"1471-2091","issn":["1471-2091"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","poland-70"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1989358564.pdf","grobid_xml":"https://content.openalex.org/works/W1989358564.grobid-xml"},"referenced_works_count":44,"referenced_works":["https://openalex.org/W57599255","https://openalex.org/W1039174517","https://openalex.org/W1414012016","https://openalex.org/W1491373961","https://openalex.org/W1547610396","https://openalex.org/W1551665537","https://openalex.org/W1772341111","https://openalex.org/W1969718038","https://openalex.org/W1971086906","https://openalex.org/W1978108133","https://openalex.org/W1981578447","https://openalex.org/W1992808956","https://openalex.org/W1994607363","https://openalex.org/W2009674410","https://openalex.org/W2010869624","https://openalex.org/W2023705609","https://openalex.org/W2023944916","https://openalex.org/W2024350932","https://openalex.org/W2033186029","https://openalex.org/W2036821989","https://openalex.org/W2044289228","https://openalex.org/W2052225744","https://openalex.org/W2061225874","https://openalex.org/W2065905475","https://openalex.org/W2067292557","https://openalex.org/W2073302085","https://openalex.org/W2073864315","https://openalex.org/W2078594804","https://openalex.org/W2079423578","https://openalex.org/W2080137815","https://openalex.org/W2092471412","https://openalex.org/W2093332482","https://openalex.org/W2095022339","https://openalex.org/W2096419043","https://openalex.org/W2100837269","https://openalex.org/W2106317935","https://openalex.org/W2106325630","https://openalex.org/W2128635872","https://openalex.org/W2148748115","https://openalex.org/W2148873746","https://openalex.org/W2149192117","https://openalex.org/W2152708819","https://openalex.org/W2168113482","https://openalex.org/W2232517365"],"related_works":["https://openalex.org/W7097287794","https://openalex.org/W3176531687","https://openalex.org/W4210607331","https://openalex.org/W4229452193","https://openalex.org/W3157773774","https://openalex.org/W2606734343","https://openalex.org/W2266200420","https://openalex.org/W3019165639","https://openalex.org/W2062967645","https://openalex.org/W2093332482"],"abstract_inverted_index":{"BACKGROUND:":[0],"Since":[1],"chitin":[2,207,228,262],"is":[3],"a":[4,84,92,107,150,248,257],"highly":[5],"abundant":[6],"natural":[7,128],"biopolymer,":[8],"many":[9],"attempts":[10],"have":[11,29],"been":[12],"made":[13],"to":[14,124,226],"convert":[15],"this":[16],"insoluble":[17],"polysaccharide":[18],"into":[19],"commercially":[20],"valuable":[21,258],"products":[22],"using":[23],"chitinases":[24,225],"and":[25,34,56,81,98,113,141,171,180,194],"\u03b2-N-acetylglucosaminidases":[26],"(GlcNAcases).":[27],"We":[28],"previously":[30],"reported":[31],"the":[32,47,53,74,127,162,182,212,233,238,243,252],"structure":[33],"function":[35],"of":[36,49,68,76,95,102,110,117,161,185,232,251],"chitinase":[37,240],"A":[38,241],"from":[39,52,73,242],"Vibrio":[40],"harveyi":[41,78],"650.":[42],"This":[43],"study":[44],"t":[45],"reports":[46],"identification":[48],"two":[50,65,234],"GlcNAcases":[51,70,121,199],"same":[54,244],"organism":[55],"their":[57],"detailed":[58],"functional":[59],"characterization.":[60],"RESULTS:":[61],"The":[62,119,230],"genes":[63],"encoding":[64],"new":[66],"members":[67],"family-20":[69],"were":[71,122,200],"isolated":[72],"genome":[75],"V.":[77],"650,":[79],"cloned":[80],"expressed":[82],"at":[83,168],"high":[85],"level":[86],"in":[87,149,260],"E.":[88],"coli.":[89],"VhNag1":[90],"has":[91,106],"molecular":[93,108],"mass":[94,109],"89":[96],"kDa":[97,112],"an":[99,114],"optimum":[100,115],"pH":[101,116],"7.5,":[103],"whereas":[104],"VhNag2":[105,130,146,186],"73":[111],"7.0.":[118],"recombinant":[120],"found":[123],"hydrolyze":[125],"all":[126],"substrates,":[129],"being":[131,156],"ten-fold":[132],"more":[133],"active":[134],"than":[135],"VhNag1.":[136],"Product":[137],"analysis":[138],"by":[139],"TLC":[140],"quantitative":[142],"HPLC":[143],"suggested":[144],"that":[145,166,181,205,247],"degraded":[147,206],"chitooligosaccharides":[148],"sequential":[151],"manner,":[152],"its":[153],"highest":[154],"activity":[155],"with":[157,237],"chitotetraose.":[158],"Kinetic":[159],"modeling":[160],"enzymic":[163],"reaction":[164],"revealed":[165],"binding":[167,176,183,190],"subsites":[169],"(-2)":[170],"(+4)":[172],"had":[173],"unfavorable":[174],"(positive)":[175],"free":[177],"energy":[178],"changes":[179],"pocket":[184],"contains":[187],"four":[188],"GlcNAc":[189,210],"subsites,":[191],"designated":[192],"(-1),(+1),(+2),":[193],"(+3).":[195],"CONCLUSIONS:":[196],"Two":[197],"novel":[198],"identified":[201],"as":[202,211],"exolytic":[203],"enzymes":[204,220,254],"oligosaccharides,":[208],"releasing":[209],"end":[213],"product.":[214],"In":[215],"living":[216],"cells,":[217],"these":[218],"intracellular":[219],"may":[221,255],"work":[222],"after":[223],"endolytic":[224],"complete":[227],"degradation.":[229],"availability":[231],"GlcNAcases,":[235],"together":[236],"previously-reported":[239],"organism,":[245],"suggests":[246],"systematic":[249],"development":[250],"chitin-degrading":[253],"provide":[256],"tool":[259],"commercial":[261],"bioconversion.":[263]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}