{"id":"https://openalex.org/W1989299971","doi":"https://doi.org/10.1021/bi00162a013","title":"DNA ligase I from Saccharomyces cerevisiae: physical and biochemical characterization of the CDC9 gene product","display_name":"DNA ligase I from Saccharomyces cerevisiae: physical and biochemical characterization of the CDC9 gene product","publication_year":1992,"publication_date":"1992-12-01","ids":{"openalex":"https://openalex.org/W1989299971","doi":"https://doi.org/10.1021/bi00162a013","mag":"1989299971","pmid":"https://pubmed.ncbi.nlm.nih.gov/1445910"},"language":"en","primary_location":{"id":"doi:10.1021/bi00162a013","doi":"https://doi.org/10.1021/bi00162a013","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00162a013","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051816777","display_name":"Alan E. Tomkinson","orcid":"https://orcid.org/0000-0002-2671-1711","observed_orcids":["https://orcid.org/0000-0002-2671-1711"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alan E. Tomkinson","raw_affiliation_strings":["Department of Pathology, University of Texas, Southwestern Medical Center, Dallas 75235-9072"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Texas, Southwestern Medical Center, Dallas 75235-9072","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045449308","display_name":"Nancy J. Tappe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nancy J. Tappe","raw_affiliation_strings":["University of Texas, Southwestern Medical Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Texas, Southwestern Medical Center","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109149946","display_name":"Errol C. Friedberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Errol C. Friedberg","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.447,"has_fulltext":false,"cited_by_count":85,"citation_normalized_percentile":{"value":0.5366832,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"31","issue":"47","first_page":"11762","last_page":"11771"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-ligase-i","display_name":"DNA ligase I","score":0.996999979019165},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9940000176429749},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.746999979019165},{"id":"https://openalex.org/keywords/protein-molecular-weight","display_name":"protein molecular weight","score":0.5820000171661377},{"id":"https://openalex.org/keywords/c-terminal-catalytic-domain","display_name":"C-terminal catalytic domain","score":0.7730000019073486},{"id":"https://openalex.org/keywords/error-prone-dna-repair","display_name":"error-prone DNA repair","score":0.8970000147819519},{"id":"https://openalex.org/keywords/dna-replication","display_name":"DNA replication","score":0.7009999752044678},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.6349999904632568},{"id":"https://openalex.org/keywords/genetic-recombination","display_name":"genetic recombination","score":0.9279999732971191}],"concepts":[{"id":"https://openalex.org/C175114707","wikidata":"https://www.wikidata.org/wiki/Q408483","display_name":"DNA ligase","level":3,"score":0.9326554536819458},{"id":"https://openalex.org/C2908738455","wikidata":"https://www.wikidata.org/wiki/Q408483","display_name":"DNA Ligases","level":4,"score":0.7719522714614868},{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.647821307182312},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5944220423698425},{"id":"https://openalex.org/C11305352","wikidata":"https://www.wikidata.org/wiki/Q845661","display_name":"Okazaki fragments","level":5,"score":0.5865459442138672},{"id":"https://openalex.org/C116988904","wikidata":"https://www.wikidata.org/wiki/Q18038809","display_name":"DNA polymerase mu","level":5,"score":0.5522465705871582},{"id":"https://openalex.org/C31254050","wikidata":"https://www.wikidata.org/wiki/Q22328799","display_name":"DNA clamp","level":5,"score":0.5324621796607971},{"id":"https://openalex.org/C47558249","wikidata":"https://www.wikidata.org/wiki/Q1422343","display_name":"Ligase chain reaction","level":5,"score":0.5123227834701538},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4967511296272278},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.4755111336708069},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.45918816328048706},{"id":"https://openalex.org/C140248907","wikidata":"https://www.wikidata.org/wiki/Q17913449","display_name":"DDB1","level":5,"score":0.4425640404224396},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.42990821599960327},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.41889768838882446},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.41082045435905457},{"id":"https://openalex.org/C134459356","wikidata":"https://www.wikidata.org/wiki/Q4274835","display_name":"Ubiquitin ligase","level":4,"score":0.36547571420669556},{"id":"https://openalex.org/C58167604","wikidata":"https://www.wikidata.org/wiki/Q5121654","display_name":"Circular bacterial chromosome","level":4,"score":0.2918558418750763},{"id":"https://openalex.org/C98086919","wikidata":"https://www.wikidata.org/wiki/Q118019","display_name":"Eukaryotic DNA replication","level":4,"score":0.27033498883247375},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.24901899695396423},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22115051746368408},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.2029283344745636},{"id":"https://openalex.org/C25602115","wikidata":"https://www.wikidata.org/wiki/Q407111","display_name":"Ubiquitin","level":3,"score":0.16707447171211243},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.06078347563743591},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.0},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000072481","descriptor_name":"DNA Ligase ATP","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002627","descriptor_name":"Chemistry, Physical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011088","descriptor_name":"DNA Ligases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011088","descriptor_name":"DNA Ligases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011088","descriptor_name":"DNA Ligases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013873","descriptor_name":"Thionucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013873","descriptor_name":"Thionucleotides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00162a013","doi":"https://doi.org/10.1021/bi00162a013","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00162a013","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:1445910","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1445910","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1170953112","display_name":null,"funder_award_id":"CA 12428","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320310169","display_name":"University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W171457159","https://openalex.org/W1482342775","https://openalex.org/W1541679067","https://openalex.org/W1574971610","https://openalex.org/W1592222398","https://openalex.org/W1604391934","https://openalex.org/W1606967916","https://openalex.org/W1611482599","https://openalex.org/W1793009834","https://openalex.org/W1932052686","https://openalex.org/W1952890385","https://openalex.org/W1971117687","https://openalex.org/W1982765928","https://openalex.org/W1989054182","https://openalex.org/W2007437374","https://openalex.org/W2010650285","https://openalex.org/W2019197460","https://openalex.org/W2019570662","https://openalex.org/W2022844346","https://openalex.org/W2028304589","https://openalex.org/W2037218720","https://openalex.org/W2043095276","https://openalex.org/W2043364265","https://openalex.org/W2045025571","https://openalex.org/W2045740938","https://openalex.org/W2047941401","https://openalex.org/W2048976298","https://openalex.org/W2067587988","https://openalex.org/W2091404614","https://openalex.org/W2092080871","https://openalex.org/W2092510099","https://openalex.org/W2095277576","https://openalex.org/W2100837269","https://openalex.org/W2110700456","https://openalex.org/W2111924246","https://openalex.org/W2116790138","https://openalex.org/W2128635872","https://openalex.org/W2159137674","https://openalex.org/W2220912830"],"related_works":["https://openalex.org/W2122247749","https://openalex.org/W1971117687","https://openalex.org/W1982765928","https://openalex.org/W2095277576","https://openalex.org/W2011312381","https://openalex.org/W2099458703","https://openalex.org/W2045067920","https://openalex.org/W2028304589","https://openalex.org/W7097124587","https://openalex.org/W2034746516"],"abstract_inverted_index":{"Genetic":[0],"studies":[1],"have":[2,41],"previously":[3],"demonstrated":[4],"that":[5,118,185],"the":[6,37,43,65,79,85,89,97,141,157,173,198,210,214,251],"Saccharomyces":[7],"cerevisiae":[8],"CDC9":[9,90],"gene":[10],"product,":[11],"which":[12],"is":[13,21,27,67,119,181],"functionally":[14],"homologous":[15],"to":[16,171,204],"mammalian":[17,122,162],"DNA":[18,24,31,93,107,123,129,154,163,174,186,194,202,234,239,245],"ligase":[19,94,124,130,155,164,175,187,203,246],"I,":[20,165],"required":[22],"for":[23,250],"replication":[25],"and":[26,33,126],"also":[28],"involved":[29,191],"in":[30,75,113,121,127,192],"repair":[32],"genetic":[34,182],"recombination.":[35],"In":[36],"present":[38],"study":[39],"we":[40],"purified":[42],"yeast":[44],"enzyme.":[45],"When":[46],"measured":[47],"under":[48],"denaturing":[49],"conditions,":[50],"Cdc9":[51,92,145,153,201],"protein":[52,146],"has":[53],"a":[54,114,228],"polypeptide":[55],"molecular":[56],"mass":[57],"of":[58,64,88,177,200,217,238,253],"87":[59],"kDa.":[60],"The":[61,109,134,236],"native":[62],"form":[63,148],"enzyme":[66,167],"an":[68,149],"80-kDa":[69],"asymmetric":[70],"monomer.":[71],"Both":[72],"estimates":[73],"are":[74],"good":[76],"agreement":[77],"with":[78,207,241],"M(r)":[80],"=":[81],"84,406":[82],"predicted":[83],"from":[84,131],"translated":[86],"sequence":[87],"gene.":[91],"acts":[95],"via":[96],"same":[98,158],"basic":[99],"reaction":[100],"mechanism":[101],"employed":[102],"by":[103,244],"all":[104],"known":[105],"ATP-dependent":[106],"ligases.":[108],"catalytic":[110],"functions":[111],"reside":[112],"70-kDa":[115],"C-terminal":[116],"domain":[117],"conserved":[120],"I":[125,176],"Cdc17":[128],"Schizosaccharomyces":[132],"pombe.":[133],"ATP":[135,137],"analog":[136],"alpha":[138],"S":[139],"inhibits":[140],"ligation":[142],"reaction,":[143],"although":[144],"does":[147],"enzyme-thioadenylate":[150],"intermediate.":[151],"Since":[152],"exhibited":[156],"substrate":[159],"specificity":[160],"as":[161],"this":[166],"can":[168],"be":[169,172,189,248],"considered":[170],"S.":[178],"cerevisiae.":[179],"There":[180],"evidence":[183],"suggesting":[184],"may":[188,247],"directly":[190],"error-prone":[193],"repair.":[195],"We":[196],"examined":[197],"ability":[199],"join":[205],"nicks":[206,218],"mismatches":[208,226],"at":[209,213,230],"termini.":[211],"Mismatches":[212],"5'":[215],"termini":[216,232,243],"had":[219],"very":[220],"little":[221],"effect":[222],"on":[223],"ligation,":[224],"whereas":[225],"opposite":[227],"purine":[229],"3'":[231],"inhibited":[233],"ligation.":[235],"joining":[237],"molecules":[240],"mismatched":[242],"responsible":[249],"generation":[252],"mutations.":[254]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}