{"id":"https://openalex.org/W1988171984","doi":"https://doi.org/10.1093/nar/3.5.1351","title":"Cleavage of T4 species I ribonucleic acid by Escherichia coli ribonuclease III","display_name":"Cleavage of T4 species I ribonucleic acid by Escherichia coli ribonuclease III","publication_year":1976,"publication_date":"1976-05-01","ids":{"openalex":"https://openalex.org/W1988171984","doi":"https://doi.org/10.1093/nar/3.5.1351","mag":"1988171984","pmid":"https://pubmed.ncbi.nlm.nih.gov/781626","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/342991"},"language":"en","primary_location":{"id":"doi:10.1093/nar/3.5.1351","doi":"https://doi.org/10.1093/nar/3.5.1351","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/3.5.1351","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC342991","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110166166","display_name":"Gary V. Paddock","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G. V. Paddock","raw_affiliation_strings":["Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030730970","display_name":"Keiko Fukada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K. Fukada","raw_affiliation_strings":["Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113673164","display_name":"John N Abelson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. Abelson","raw_affiliation_strings":["Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5114001819","display_name":"Hugh D. Robertson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H. D. Robertson","raw_affiliation_strings":["Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of California San Diego, La Jolla, CA 92093, USA","institution_ids":["https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I36258959"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":1.1223,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.73356376,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"3","issue":"5","first_page":"1351","last_page":"1372"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.8988999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.8988999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.06300000101327896,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.01489999983459711,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-cleavage","display_name":"RNA cleavage","score":0.824999988079071},{"id":"https://openalex.org/keywords/rna-processing","display_name":"RNA processing","score":0.6439999938011169},{"id":"https://openalex.org/keywords/ionic-strength-dependence","display_name":"ionic strength dependence","score":0.6589999794960022},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.609000027179718}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9366597533226013},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.8196970820426941},{"id":"https://openalex.org/C2778976237","wikidata":"https://www.wikidata.org/wiki/Q422523","display_name":"Ribonuclease","level":4,"score":0.7203214168548584},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.6619900465011597},{"id":"https://openalex.org/C2908821069","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli Proteins","level":4,"score":0.638076663017273},{"id":"https://openalex.org/C79750893","wikidata":"https://www.wikidata.org/wiki/Q3430180","display_name":"Ribonuclease III","level":5,"score":0.617213249206543},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.47798746824264526},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43577277660369873},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4049157202243805},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3358476161956787},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1284676194190979},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002413","descriptor_name":"Cations, Divalent","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002413","descriptor_name":"Cations, Divalent","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002413","descriptor_name":"Cations, Divalent","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/3.5.1351","doi":"https://doi.org/10.1093/nar/3.5.1351","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/3.5.1351","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:43728","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC342991","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:342991","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/342991","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:781626","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/781626","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:43728","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC342991","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.5199999809265137,"id":"https://metadata.un.org/sdg/15","display_name":"Life on land"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1486502097","https://openalex.org/W1506699506","https://openalex.org/W1514335381","https://openalex.org/W1528919862","https://openalex.org/W1646291673","https://openalex.org/W1735184170","https://openalex.org/W1750773183","https://openalex.org/W1775749144","https://openalex.org/W1966188128","https://openalex.org/W1981777358","https://openalex.org/W2017302704","https://openalex.org/W2022984740","https://openalex.org/W2024842562","https://openalex.org/W2035256214","https://openalex.org/W2036992576","https://openalex.org/W2093838158","https://openalex.org/W2460406834"],"related_works":["https://openalex.org/W208623515","https://openalex.org/W1584261210","https://openalex.org/W1581724194","https://openalex.org/W1486502097","https://openalex.org/W1646291673","https://openalex.org/W1971143693","https://openalex.org/W1002073993","https://openalex.org/W2497063143","https://openalex.org/W1528919862","https://openalex.org/W4318599963"],"abstract_inverted_index":{"T4":[0,58,83,114],"Species":[1,59,84,115],"I":[2,60,85,116],"RNA,":[3],"a":[4,17],"molecule":[5],"140":[6],"nucleotides":[7],"in":[8,26,67],"length":[9],"with":[10,34,69],"some":[11],"structural":[12],"features":[13],"very":[14],"much":[15],"like":[16],"tRNA,":[18],"is":[19,99,118,140],"specifically":[20],"cleaved":[21,119],"by":[22,72,102,120],"an":[23,105],"enzymatic":[24],"activity":[25],"Escherichia":[27],"coli":[28,49,74,107,124],"extracts":[29,103],"to":[30,127,142],"give":[31],"three":[32,131],"segments":[33],"19,":[35],"48":[36],"and":[37,44,148],"73":[38],"nucleotides.":[39],"We":[40,134],"report":[41],"the":[42,47,78,129,143,150],"purification":[43],"characterization":[45],"of":[46,57,145],"E.":[48,73,106,123],"RNase":[50,75,94,110,125,146],"which":[51],"cleaves":[52],"two":[53],"3'":[54],"phosphodiester":[55],"bonds":[56],"RNA.":[61],"This":[62],"reaction":[63,98],"has":[64],"many":[65],"properties":[66,160],"common":[68],"those":[70,91],"catalyzed":[71,101],"III,":[76,147],"although":[77],"optimal":[79],"salt":[80],"conditions":[81],"for":[82,92,152],"RNA":[86,117,163],"cleavage":[87,139],"differ":[88],"significantly":[89],"from":[90,104],"other":[93],"III-catalyzed":[95],"reactions.":[96],"The":[97],"not":[100],"strain":[108],"lacking":[109],"III":[111,126],"activity.":[112],"Furthermore,":[113],"highly":[121],"purified":[122],"yield":[128],"same":[130],"specific":[132,138],"fragments.":[133],"conclude":[135],"that":[136,149],"this":[137,162],"due":[141],"action":[144],"requirement":[151],"lower":[153],"ionic":[154],"strength":[155],"may":[156],"reveal":[157],"further":[158],"important":[159],"about":[161],"processing":[164],"enzyme.":[165]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}