{"id":"https://openalex.org/W1986550650","doi":"https://doi.org/10.1073/pnas.70.2.385","title":"Regulation of Macromolecular Biosynthesis in a Mutant of Escherichia coli Defective in Membrane Phospholipid Biosynthesis","display_name":"Regulation of Macromolecular Biosynthesis in a Mutant of Escherichia coli Defective in Membrane Phospholipid Biosynthesis","publication_year":1973,"publication_date":"1973-02-01","ids":{"openalex":"https://openalex.org/W1986550650","doi":"https://doi.org/10.1073/pnas.70.2.385","mag":"1986550650","pmid":"https://pubmed.ncbi.nlm.nih.gov/4568726","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/433265"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.70.2.385","doi":"https://doi.org/10.1073/pnas.70.2.385","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.70.2.385","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC433265/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109234857","display_name":"Michael Gl\u00e4ser","orcid":"https://orcid.org/0000-0001-8843-1662","observed_orcids":["https://orcid.org/0000-0001-8843-1662"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Glaser","raw_affiliation_strings":["Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081727371","display_name":"William H. Bayer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William H. Bayer","raw_affiliation_strings":["Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Robert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert","raw_affiliation_strings":["Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":null,"display_name":"M. Bell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Bell","raw_affiliation_strings":["Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075976228","display_name":"P. Roy Vagelos","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. Roy Vagelos","raw_affiliation_strings":["Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204465549"],"apc_list":null,"apc_paid":null,"fwci":5.9,"has_fulltext":false,"cited_by_count":47,"citation_normalized_percentile":{"value":0.98369565,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"70","issue":"2","first_page":"385","last_page":"389"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.8471999764442444,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.8471999764442444,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.11550000309944153,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.026599999517202377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glycerol-3-phosphate-acyltransferase","display_name":"glycerol-3-phosphate acyltransferase","score":0.9580000042915344},{"id":"https://openalex.org/keywords/temperature-sensitive-mutants","display_name":"temperature-sensitive mutants","score":0.8450000286102295},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8610000014305115},{"id":"https://openalex.org/keywords/macromolecular-synthesis","display_name":"macromolecular synthesis","score":0.7799999713897705},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.9120000004768372},{"id":"https://openalex.org/keywords/rna-synthesis","display_name":"RNA synthesis","score":1.0},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/active-transport","display_name":"active transport","score":0.7649999856948853},{"id":"https://openalex.org/keywords/metabolic-coupling","display_name":"metabolic coupling","score":0.35499998927116394}],"concepts":[{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.7092146873474121},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6659602522850037},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.6621202230453491},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6314706802368164},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.6233969926834106},{"id":"https://openalex.org/C2775970874","wikidata":"https://www.wikidata.org/wiki/Q2609152","display_name":"Acyltransferase","level":3,"score":0.5587545037269592},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5499300360679626},{"id":"https://openalex.org/C64241487","wikidata":"https://www.wikidata.org/wiki/Q5205774","display_name":"DNA synthesis","level":3,"score":0.5484902262687683},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.547904908657074},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5314067006111145},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.48251888155937195},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4607381224632263},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3678404688835144},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.30532461404800415},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14894229173660278},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1259329617023468}],"mesh":[{"descriptor_ui":"D000217","descriptor_name":"Acyltransferases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D002247","descriptor_name":"Carbon Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005994","descriptor_name":"Glycerophosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006027","descriptor_name":"Glycosides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007930","descriptor_name":"Leucine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008566","descriptor_name":"Membranes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010743","descriptor_name":"Phospholipids","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D010759","descriptor_name":"Phosphorus Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014498","descriptor_name":"Uracil","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1073/pnas.70.2.385","doi":"https://doi.org/10.1073/pnas.70.2.385","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.70.2.385","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmid:4568726","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4568726","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:433265","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC433265/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"mag:2528760151","doi":null,"is_oa":false,"landing_page_url":"https://www.jstor.org/stable/62503","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:433265","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC433265/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W147125004","https://openalex.org/W228204195","https://openalex.org/W575151505","https://openalex.org/W649837179","https://openalex.org/W997127808","https://openalex.org/W1493354657","https://openalex.org/W1493567759","https://openalex.org/W1502553870","https://openalex.org/W1518564811","https://openalex.org/W1527526272","https://openalex.org/W1532864556","https://openalex.org/W1534716784","https://openalex.org/W1538191882","https://openalex.org/W1543463078","https://openalex.org/W1548129143","https://openalex.org/W1549586164","https://openalex.org/W1560189124","https://openalex.org/W1568532879","https://openalex.org/W1587708054","https://openalex.org/W1588544522","https://openalex.org/W1783238189","https://openalex.org/W1908664420","https://openalex.org/W1925411479","https://openalex.org/W1980703147","https://openalex.org/W1991655565","https://openalex.org/W2005382790","https://openalex.org/W2015295364","https://openalex.org/W2017016334","https://openalex.org/W2031785241","https://openalex.org/W2039795860","https://openalex.org/W2057078460","https://openalex.org/W2063115679","https://openalex.org/W2063367203","https://openalex.org/W2070108182","https://openalex.org/W2076513198","https://openalex.org/W2083221094","https://openalex.org/W2097335319","https://openalex.org/W2125008573","https://openalex.org/W2131974594","https://openalex.org/W2152311269","https://openalex.org/W2166153420","https://openalex.org/W2168377910","https://openalex.org/W4323285587"],"related_works":["https://openalex.org/W2528760151","https://openalex.org/W113576763","https://openalex.org/W1530607584","https://openalex.org/W2043827740","https://openalex.org/W2057078460","https://openalex.org/W1518776709","https://openalex.org/W1675862931","https://openalex.org/W1546910772","https://openalex.org/W3146325233","https://openalex.org/W2015295364"],"abstract_inverted_index":{"Nucleic":[0],"acid":[1],"and":[2,45,78],"protein":[3,46,79],"synthesis":[4,40,47,72],"were":[5],"studied":[6],"in":[7,11,22],"temperature-sensitive":[8],"mutants":[9],"defective":[10],"phospholipid":[12,39,71],"synthesis.":[13,80],"The":[14,28],"defect":[15],"is":[16,73],"due":[17],"to":[18,75],"a":[19],"single":[20],"mutation":[21],"glycerol":[23],"3-phosphate":[24],"acyltransferase":[25],"(EC":[26],"2.3.1.15).":[27],"results":[29,68],"show":[30],"that":[31,55,70],"at":[32],"the":[33,56],"restrictive":[34],"temperature":[35],"not":[36,59],"only":[37],"does":[38],"cease,":[41],"but":[42],"DNA,":[43,76],"RNA,":[44,77],"also":[48],"cease.":[49],"Active":[50],"transport":[51],"continues,":[52],"however,":[53],"indicating":[54],"cells":[57],"do":[58],"become":[60],"leaky":[61],"or":[62],"lose":[63],"their":[64],"energy":[65],"supply.":[66],"These":[67],"suggest":[69],"coupled":[74]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}