{"id":"https://openalex.org/W1985776865","doi":"https://doi.org/10.1080/0967026032000157156","title":"Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors","display_name":"Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors","publication_year":2004,"publication_date":"2004-02-01","ids":{"openalex":"https://openalex.org/W1985776865","doi":"https://doi.org/10.1080/0967026032000157156","mag":"1985776865"},"language":"en","primary_location":{"id":"doi:10.1080/0967026032000157156","doi":"https://doi.org/10.1080/0967026032000157156","is_oa":true,"landing_page_url":"https://doi.org/10.1080/0967026032000157156","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1080/0967026032000157156?needAccess=true","source":{"id":"https://openalex.org/S133660675","display_name":"European Journal of Phycology","issn_l":"0967-0262","issn":["0967-0262","1469-4433"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Phycology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.tandfonline.com/doi/pdf/10.1080/0967026032000157156?needAccess=true","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103415165","display_name":"Sergio O. Louren\u00e7o","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161127581","display_name":"Universidade Federal Fluminense","ror":"https://ror.org/02rjhbb08","country_code":"BR","type":"education","lineage":["https://openalex.org/I161127581"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Sergio O Louren\u00e7o","raw_affiliation_strings":["Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, Niter\u00f3i, RJ, Brazil, CEP 24001-970"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, Niter\u00f3i, RJ, Brazil, CEP 24001-970","institution_ids":["https://openalex.org/I161127581"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017184520","display_name":"Elisabete Barbarino","orcid":"https://orcid.org/0000-0002-5037-5113","observed_orcids":["https://orcid.org/0000-0002-5037-5113"]},"institutions":[{"id":"https://openalex.org/I161127581","display_name":"Universidade Federal Fluminense","ror":"https://ror.org/02rjhbb08","country_code":"BR","type":"education","lineage":["https://openalex.org/I161127581"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Elisabete Barbarino","raw_affiliation_strings":["Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, Niter\u00f3i, RJ, Brazil, CEP 24001-970"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, Niter\u00f3i, RJ, Brazil, CEP 24001-970","institution_ids":["https://openalex.org/I161127581"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058149810","display_name":"Paris Leonardo Lavin","orcid":"https://orcid.org/0000-0002-8893-526X","observed_orcids":["https://orcid.org/0000-0002-8893-526X"]},"institutions":[{"id":"https://openalex.org/I161127581","display_name":"Universidade Federal Fluminense","ror":"https://ror.org/02rjhbb08","country_code":"BR","type":"education","lineage":["https://openalex.org/I161127581"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Paris L Lav\u00edn","raw_affiliation_strings":["Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, Niter\u00f3i, RJ, Brazil, CEP 24001-970"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, Niter\u00f3i, RJ, Brazil, CEP 24001-970","institution_ids":["https://openalex.org/I161127581"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026105730","display_name":"\u00darsula Maria Lanfer Marquez","orcid":"https://orcid.org/0000-0002-0291-6333","observed_orcids":["https://orcid.org/0000-0002-0291-6333"]},"institutions":[{"id":"https://openalex.org/I17974374","display_name":"Universidade de S\u00e3o Paulo","ror":"https://ror.org/036rp1748","country_code":"BR","type":"education","lineage":["https://openalex.org/I17974374"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Ursula M Lanfer Marquez","raw_affiliation_strings":["Departamento de Alimentos e Nutri\u00e7\u00e3o Experimental, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo, Caixa Postal 66083, S\u00e3o Paulo, SP, Brazil, CEP 05315-970"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Alimentos e Nutri\u00e7\u00e3o Experimental, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo, Caixa Postal 66083, S\u00e3o Paulo, SP, Brazil, CEP 05315-970","institution_ids":["https://openalex.org/I17974374"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112471431","display_name":"Elizabeth Aidar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17974374","display_name":"Universidade de S\u00e3o Paulo","ror":"https://ror.org/036rp1748","country_code":"BR","type":"education","lineage":["https://openalex.org/I17974374"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Elizabeth Aidar","raw_affiliation_strings":["Departamento de Oceanografia Biol\u00f3gica, Instituto Oceanogr\u00e1fico, Universidade de S\u00e3o Paulo, Caixa Postal 66149, S\u00e3o Paulo, SP, Brazil, CEP 05315-970"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Oceanografia Biol\u00f3gica, Instituto Oceanogr\u00e1fico, Universidade de S\u00e3o Paulo, Caixa Postal 66149, S\u00e3o Paulo, SP, Brazil, CEP 05315-970","institution_ids":["https://openalex.org/I17974374"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.0211,"has_fulltext":true,"cited_by_count":509,"citation_normalized_percentile":{"value":0.69482379,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"39","issue":"1","first_page":"17","last_page":"32"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10032","display_name":"Marine Plankton and Biogeochemistry","score":0.6083999872207642,"subfield":{"id":"https://openalex.org/subfields/1910","display_name":"Oceanography"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10032","display_name":"Marine Plankton and Biogeochemistry","score":0.6083999872207642,"subfield":{"id":"https://openalex.org/subfields/1910","display_name":"Oceanography"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10476","display_name":"Algal biology and biofuel production","score":0.3594000041484833,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10236","display_name":"Aquatic Ecosystems and Phytoplankton Dynamics","score":0.0210999995470047,"subfield":{"id":"https://openalex.org/subfields/2304","display_name":"Environmental Chemistry"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/marine-microalgae","display_name":"marine microalgae","score":0.8899999856948853},{"id":"https://openalex.org/keywords/nitrogen-to-protein-conversion-factor","display_name":"nitrogen-to-protein conversion factor","score":0.9169999957084656},{"id":"https://openalex.org/keywords/protein-quantification","display_name":"protein quantification","score":0.6869999766349792},{"id":"https://openalex.org/keywords/non-protein-nitrogen","display_name":"non-protein nitrogen","score":0.890999972820282},{"id":"https://openalex.org/keywords/inorganic-nitrogen","display_name":"inorganic nitrogen","score":0.7229999899864197},{"id":"https://openalex.org/keywords/nucleic-acids","display_name":"nucleic acids","score":0.8889999985694885},{"id":"https://openalex.org/keywords/nitrogen-balance","display_name":"nitrogen balance","score":0.7059999704360962},{"id":"https://openalex.org/keywords/nitrogen-starvation","display_name":"nitrogen starvation","score":0.6800000071525574},{"id":"https://openalex.org/keywords/aeration","display_name":"aeration","score":0.8289999961853027},{"id":"https://openalex.org/keywords/growth-phase","display_name":"growth phase","score":0.6380000114440918},{"id":"https://openalex.org/keywords/amino-acid-residues","display_name":"amino acid residues","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C86922832","wikidata":"https://www.wikidata.org/wiki/Q381105","display_name":"Aeration","level":2,"score":0.7873420715332031},{"id":"https://openalex.org/C537208039","wikidata":"https://www.wikidata.org/wiki/Q627","display_name":"Nitrogen","level":2,"score":0.7837488651275635},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6848295331001282},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.6026479601860046},{"id":"https://openalex.org/C2780417740","wikidata":"https://www.wikidata.org/wiki/Q7048993","display_name":"Non-protein nitrogen","level":3,"score":0.47068268060684204},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.42836347222328186},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3432561755180359},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.33722826838493347},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.18228048086166382},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.16105937957763672},{"id":"https://openalex.org/C2780365088","wikidata":"https://www.wikidata.org/wiki/Q48318","display_name":"Urea","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1080/0967026032000157156","doi":"https://doi.org/10.1080/0967026032000157156","is_oa":true,"landing_page_url":"https://doi.org/10.1080/0967026032000157156","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1080/0967026032000157156?needAccess=true","source":{"id":"https://openalex.org/S133660675","display_name":"European Journal of Phycology","issn_l":"0967-0262","issn":["0967-0262","1469-4433"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Phycology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1080/0967026032000157156","doi":"https://doi.org/10.1080/0967026032000157156","is_oa":true,"landing_page_url":"https://doi.org/10.1080/0967026032000157156","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1080/0967026032000157156?needAccess=true","source":{"id":"https://openalex.org/S133660675","display_name":"European Journal of Phycology","issn_l":"0967-0262","issn":["0967-0262","1469-4433"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Phycology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320997","display_name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","ror":"https://ror.org/02ddkpn78"},{"id":"https://openalex.org/F4320321832","display_name":"Queen's University","ror":"https://ror.org/02y72wh86"},{"id":"https://openalex.org/F4320322025","display_name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","ror":"https://ror.org/03swz6y49"},{"id":"https://openalex.org/F4320322749","display_name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","ror":"https://ror.org/03kk0s825"},{"id":"https://openalex.org/F4320325035","display_name":"Universidade Federal do Rio de Janeiro","ror":"https://ror.org/03490as77"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1985776865.pdf","grobid_xml":"https://content.openalex.org/works/W1985776865.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W1542162133","https://openalex.org/W1549492716","https://openalex.org/W1573664132","https://openalex.org/W1575487276","https://openalex.org/W1579790471","https://openalex.org/W1775749144","https://openalex.org/W1968927116","https://openalex.org/W1980798794","https://openalex.org/W1983193389","https://openalex.org/W1983787386","https://openalex.org/W1985392516","https://openalex.org/W1986163328","https://openalex.org/W1986802534","https://openalex.org/W2000743242","https://openalex.org/W2009315810","https://openalex.org/W2012805152","https://openalex.org/W2016419639","https://openalex.org/W2017651867","https://openalex.org/W2024921796","https://openalex.org/W2027900136","https://openalex.org/W2035787023","https://openalex.org/W2041961760","https://openalex.org/W2050133547","https://openalex.org/W2073203076","https://openalex.org/W2077579330","https://openalex.org/W2086316039","https://openalex.org/W2097915571","https://openalex.org/W2107377108","https://openalex.org/W2128635872","https://openalex.org/W2140841303","https://openalex.org/W2147937413","https://openalex.org/W2152514946","https://openalex.org/W2152848489","https://openalex.org/W2239384431","https://openalex.org/W2405763898","https://openalex.org/W2437230716","https://openalex.org/W2475112939","https://openalex.org/W2554491009","https://openalex.org/W2625480029","https://openalex.org/W4232036084","https://openalex.org/W4246444229","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2041961760","https://openalex.org/W3026088017","https://openalex.org/W2089091727","https://openalex.org/W7161179907","https://openalex.org/W2127816351","https://openalex.org/W2893797186","https://openalex.org/W3141228835","https://openalex.org/W2035787023","https://openalex.org/W2163717142","https://openalex.org/W4387429754"],"abstract_inverted_index":{"Nitrogen":[0],"budgets":[1,163],"in":[2,30,33,122,168,181,190,195,224,250],"microalgae":[3,31,119],"are":[4,76,267],"strongly":[5],"affected":[6],"by":[7,62,161,228],"growth":[8,102,170,197,313],"conditions":[9],"and":[10,36,73,137,139,151,186,194,207,236,296,312,338],"physiological":[11],"state":[12],"of":[13,42,53,59,69,95,103,106,156,164,172,184,222,234,241,246,263,271,279,315,328,341],"the":[14,40,43,49,67,86,93,96,101,173,182,191,214,251,272,277,298,326,329,339,342],"cultures.":[15,174],"As":[16],"a":[17,83,128,142],"consequence,":[18],"protein":[19,55,60],"N":[20,24,133,147,188],"(PN)":[21],"to":[22,78,91,112,261,283,290],"total":[23],"(TN)":[25],"ratio":[26,278],"may":[27,38],"be":[28],"variable":[29],"grown":[32],"batch":[34],"cultures,":[35],"this":[37,63,80],"limit":[39],"usefulness":[41],"nitrogen-to-protein":[44],"conversion":[45],"factors":[46,115,266,344],"(N-Prot":[47],"factors),":[48],"most":[50,215],"practical":[51],"way":[52],"determining":[54],"content.":[56],"The":[57,118,154,243],"accuracy":[58],"determination":[61],"method":[64],"depends":[65],"on":[66,276,293,297],"establishment":[68],"specific":[70],"N-Prot":[71,114,265,306,343],"factors,":[72],"experimental":[74,124],"data":[75],"needed":[77],"fill":[79],"gap.":[81],"Complementing":[82],"previous":[84,299],"study,":[85,300],"present":[87],"work":[88],"was":[89,159,213,248],"designed":[90],"quantify":[92],"fluctuations":[94],"main":[97],"nitrogenous":[98],"compounds":[99],"during":[100,255],"12":[104],"species":[105,273],"marine":[107,336],"microalgae,":[108,337],"as":[109,111],"well":[110],"determine":[113],"for":[116,269,308,335],"them.":[117],"were":[120],"cultured":[121],"two":[123],"conditions:":[125],"(a)":[126],"using":[127],"N-replete":[129],"culture":[130,144],"medium":[131,145],"(initial":[132,146],"concentration,":[134,148],"1.18":[135],"mM)":[136],"aeration,":[138],"(b)":[140],"with":[141,285],"N-depleted":[143],"235":[149],"\u03bcM)":[150],"no":[152],"aeration.":[153],"distribution":[155,183],"intracellular":[157],"nitrogen":[158,166,201],"studied":[160],"constructing":[162],"different":[165,169,196],"pools":[167],"phases":[171,314],"In":[175],"all":[176,225,309],"species,":[177,226,310],"large":[178],"variations":[179],"occurred":[180],"PN":[185,257],"non-protein":[187],"(NPN)":[189],"treatments":[192,311],"tested":[193],"phases.":[198],"Intracellular":[199],"inorganic":[200],"(NO3":[202],"\u2212":[203,206],",":[204],"NO2":[205],"NH3":[208],"+":[209,211],"NH4":[210],")":[212],"important":[216],"NPN":[217,247],"component":[218],"(0.4":[219],"\u2013":[220,232,239],"30.4%":[221],"TN)":[223],"followed":[227],"nucleic":[229],"acids":[230],"(0.3":[231],"12.2%":[233],"TN),":[235],"chlorophylls":[237],"(0.1":[238],"1.8%":[240],"TN).":[242],"relative":[244],"importance":[245],"greater":[249],"exponential":[252],"phase,":[253],"decreasing":[254],"growth.":[256],"ranged":[258],"from":[259,288],"59.3":[260],"96.8%":[262],"TN.":[264],"proposed":[268,345],"each":[270],"studied,":[274],"based":[275],"amino":[280],"acid":[281],"residues":[282],"TN,":[284],"values":[286],"ranging":[287],"2.53":[289],"5.77.":[291],"Based":[292],"current":[294],"results":[295],"we":[301],"establish":[302],"an":[303],"overall":[304],"average":[305],"factor":[307,331],"4.78":[316],"\u00b1":[317],"0.62":[318],"(n":[319],"=":[320],"354).":[321],"This":[322],"study":[323],"confirms":[324],"that":[325],"use":[327,340],"traditional":[330],"6.25":[332],"is":[333,347],"unsuitable":[334],"here":[346],"recommended.":[348]},"counts_by_year":[{"year":2026,"cited_by_count":36},{"year":2025,"cited_by_count":45},{"year":2024,"cited_by_count":29},{"year":2023,"cited_by_count":35},{"year":2022,"cited_by_count":52},{"year":2021,"cited_by_count":45},{"year":2020,"cited_by_count":38},{"year":2019,"cited_by_count":46},{"year":2018,"cited_by_count":37},{"year":2017,"cited_by_count":24},{"year":2016,"cited_by_count":36},{"year":2015,"cited_by_count":18},{"year":2014,"cited_by_count":20},{"year":2013,"cited_by_count":18},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}