{"id":"https://openalex.org/W1985443566","doi":"https://doi.org/10.3109/10520297709116774","title":"In VitroLabeling of Solid Tissues with Tritiated Thymidine for Autoradiographic Detection of S-Phase Nuclei","display_name":"In VitroLabeling of Solid Tissues with Tritiated Thymidine for Autoradiographic Detection of S-Phase Nuclei","publication_year":1977,"publication_date":"1977-01-01","ids":{"openalex":"https://openalex.org/W1985443566","doi":"https://doi.org/10.3109/10520297709116774","mag":"1985443566","pmid":"https://pubmed.ncbi.nlm.nih.gov/143737"},"language":"en","primary_location":{"id":"doi:10.3109/10520297709116774","doi":"https://doi.org/10.3109/10520297709116774","is_oa":false,"landing_page_url":"https://doi.org/10.3109/10520297709116774","pdf_url":null,"source":{"id":"https://openalex.org/S4210226617","display_name":"Stain Technology","issn_l":"0038-9153","issn":["0038-9153"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stain Technology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112763050","display_name":"John Strauch Meyer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John S. Meyer","raw_affiliation_strings":["Department of Pathology, Washington University School of Medicine, and the Jewish Hospital of St. Louis, St Loub, Missouri, 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Washington University School of Medicine, and the Jewish Hospital of St. Louis, St Loub, Missouri, 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048627938","display_name":"Robert E. Connor","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert E. Connor","raw_affiliation_strings":["Department of Pathology, Washington University School of Medicine, and the Jewish Hospital of St. Louis, St Loub, Missouri, 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Washington University School of Medicine, and the Jewish Hospital of St. Louis, St Loub, Missouri, 63110","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204465549"],"apc_list":null,"apc_paid":null,"fwci":0.9177,"has_fulltext":false,"cited_by_count":67,"citation_normalized_percentile":{"value":0.67363119,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"52","issue":"4","first_page":"185","last_page":"195"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14135","display_name":"Cancer and biochemical research","score":0.2809999883174896,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14135","display_name":"Cancer and biochemical research","score":0.2809999883174896,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.13809999823570251,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13561","display_name":"Chemical Reactions and Isotopes","score":0.06520000100135803,"subfield":{"id":"https://openalex.org/subfields/3003","display_name":"Pharmaceutical Science"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thymidine-labeling-index","display_name":"thymidine labeling index","score":0.9459999799728394},{"id":"https://openalex.org/keywords/tritiated-thymidine","display_name":"tritiated thymidine","score":0.9459999799728394},{"id":"https://openalex.org/keywords/hyperbaric-oxygen-therapy","display_name":"hyperbaric oxygen therapy","score":0.9610000252723694},{"id":"https://openalex.org/keywords/5-fluorodeoxyuridine","display_name":"5-fluorodeoxyuridine","score":0.9380000233650208},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.7360000014305115},{"id":"https://openalex.org/keywords/autoradiography","display_name":"autoradiography","score":0.8309999704360962},{"id":"https://openalex.org/keywords/needle-biopsy","display_name":"needle biopsy","score":0.8199999928474426}],"concepts":[{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.8603918552398682},{"id":"https://openalex.org/C2777111502","wikidata":"https://www.wikidata.org/wiki/Q419140","display_name":"Deoxyuridine","level":3,"score":0.581243634223938},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.5092705488204956},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49052709341049194},{"id":"https://openalex.org/C2778996431","wikidata":"https://www.wikidata.org/wiki/Q4849997","display_name":"Balanced salt solution","level":2,"score":0.46270468831062317},{"id":"https://openalex.org/C25642318","wikidata":"https://www.wikidata.org/wiki/Q1992532","display_name":"Incubation","level":2,"score":0.460042268037796},{"id":"https://openalex.org/C70289976","wikidata":"https://www.wikidata.org/wiki/Q17143603","display_name":"Oxygenation","level":2,"score":0.44826847314834595},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4317816495895386},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.351873517036438},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26823890209198},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23449721932411194},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002453","descriptor_name":"Cell Cycle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003080","descriptor_name":"Cold Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005467","descriptor_name":"Floxuridine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006652","descriptor_name":"Histological Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006931","descriptor_name":"Hyperbaric Oxygenation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008325","descriptor_name":"Mammary Neoplasms, Experimental","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.3109/10520297709116774","doi":"https://doi.org/10.3109/10520297709116774","is_oa":false,"landing_page_url":"https://doi.org/10.3109/10520297709116774","pdf_url":null,"source":{"id":"https://openalex.org/S4210226617","display_name":"Stain Technology","issn_l":"0038-9153","issn":["0038-9153"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stain Technology","raw_type":"journal-article"},{"id":"pmid:143737","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/143737","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stain technology","raw_type":"Journal Article"},{"id":"pmh:oai:mouseion.jax.org:ssbb1977-2227","doi":null,"is_oa":false,"landing_page_url":"https://mouseion.jax.org/ssbb1977/1228","pdf_url":null,"source":{"id":"https://openalex.org/S4377196421","display_name":"The Mouseion at the JAXlibrary (Jackson Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210115256","host_organization_name":"Jackson Laboratory","host_organization_lineage":["https://openalex.org/I4210115256"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Subject Strain Bibliography 1977","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7246000170707703}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W53300029","https://openalex.org/W118964469","https://openalex.org/W2002899214","https://openalex.org/W2010019455","https://openalex.org/W2012355463","https://openalex.org/W2026326930","https://openalex.org/W2050644086","https://openalex.org/W2084456057","https://openalex.org/W2088164628","https://openalex.org/W2099970702","https://openalex.org/W2158139535","https://openalex.org/W2165949836"],"related_works":["https://openalex.org/W2061475862","https://openalex.org/W2064470126","https://openalex.org/W2412411823","https://openalex.org/W432707124","https://openalex.org/W1683709387","https://openalex.org/W1482766550","https://openalex.org/W2151702474","https://openalex.org/W7095642268","https://openalex.org/W14733318","https://openalex.org/W2039784444"],"abstract_inverted_index":{"In":[0],"vitro":[1],"measurement":[2,107],"of":[3,9,20,29,44,72,101,110,119,137,148,154,158,167],"the":[4,125,143,146,149,165,168,194],"thymidine":[5,31],"labeling":[6,138,155],"index":[7],"(TLI)":[8],"solid":[10],"tissues":[11],"requires":[12],"hyperbaric":[13,183],"oxygenation":[14,34],"and":[15,124],"is":[16,76,85,103,121,133,140],"potentiated":[17],"by":[18,23,177],"blockade":[19],"thymidylate":[21],"synthetase":[22],"5-fluoro-2'-deoxyuridine":[24],"(FUdR)":[25],"to":[26,95,145],"favor":[27],"uptake":[28],"tritiated":[30],"(3H-TdR).":[32],"Hyperbaric":[33],"can":[35,161,172],"be":[36,162,173],"achieved":[37,163],"in":[38,54,59,129,164,180],"a":[39,60],"simple":[40],"system":[41],"through":[42],"injection":[43],"oxygen":[45,98,184],"into":[46],"rubber-stoppered":[47],"test":[48],"tubes.":[49],"Incubations":[50],"are":[51,201],"carried":[52],"out":[53],"Hanks'":[55],"balanced":[56],"salt":[57],"solution":[58],"shaker":[61],"bath":[62],"at":[63,185],"37":[64],"C":[65],"for":[66,80,87,105,156,203],"2":[67],"hours;":[68],"an":[69],"FUdR":[70],"concentration":[71],"approximately":[73],"1":[74,81,113],"micron":[75],"optimal.":[77],"Autoradiographic":[78],"exposure":[79],"week":[82],"or":[83,116],"less":[84],"sufficient":[86,104,152],"TLI":[88,106,191],"measurements":[89,192],"on":[90,193],"human":[91],"tissues.":[92],"With":[93],"3":[94],"4":[96],"atmospheres":[97],"tension,":[99],"incorporation":[100],"3H-TdR":[102,181],"throughout":[108],"slices":[109],"tissue":[111,120],"cut":[112],"mm":[114],"thick":[115],"less.":[117],"Mincing":[118],"not":[122],"necessary,":[123],"anatomic":[126],"continuity":[127],"seen":[128],"ordinary":[130],"histological":[131],"preparations":[132],"preserved.":[134],"A":[135],"gradient":[136,171],"intensity":[139,153],"present":[141],"from":[142],"surface":[144],"interior":[147,166],"tissue,":[150],"but":[151],"detection":[157],"DNA":[159],"synthesis":[160],"section.":[169],"The":[170,188],"reduced":[174],"only":[175],"slightly":[176],"prior":[178],"incubation":[179],"with":[182],"0":[186],"C.":[187],"method":[189],"permits":[190],"same":[195],"specimens,":[196],"including":[197],"needle":[198],"biopsies,":[199],"that":[200],"used":[202],"pathologic":[204],"diagnosis.":[205]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}