{"id":"https://openalex.org/W1985349579","doi":"https://doi.org/10.1046/j.1365-2818.2003.01100.x","title":"Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser","display_name":"Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser","publication_year":2003,"publication_date":"2003-01-01","ids":{"openalex":"https://openalex.org/W1985349579","doi":"https://doi.org/10.1046/j.1365-2818.2003.01100.x","mag":"1985349579","pmid":"https://pubmed.ncbi.nlm.nih.gov/12535185"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2818.2003.01100.x","doi":"https://doi.org/10.1046/j.1365-2818.2003.01100.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2818.2003.01100.x","pdf_url":null,"source":{"id":"https://openalex.org/S168975578","display_name":"Journal of Microscopy","issn_l":"0022-2720","issn":["0022-2720","1365-2818"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Microscopy","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046535878","display_name":"Tatiana Karpova","orcid":"https://orcid.org/0000-0001-6025-2128","observed_orcids":["https://orcid.org/0000-0001-6025-2128"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. S. Karpova","raw_affiliation_strings":["CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI, National Institutes of Health, Bethesda, MD 20892, USA","CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI, National Institutes of Health, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]},{"raw_affiliation_string":"CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,","institution_ids":["https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}]},{"author_position":"middle","author":{"id":null,"display_name":"C. T. Baumann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. T. Baumann","raw_affiliation_strings":["CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,","Present address Chroma Technology Corporation, 74 Cotton Mill Hill, Unit A9, Brattleboro VT 05301, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,","institution_ids":["https://openalex.org/I4210140884","https://openalex.org/I4210149717"]},{"raw_affiliation_string":"Present address Chroma Technology Corporation, 74 Cotton Mill Hill, Unit A9, Brattleboro VT 05301, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"L. He","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210087962","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L. He","raw_affiliation_strings":["Flow Cytometry Section, Office of Science and Technology, NIAMS and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Flow Cytometry Section, Office of Science and Technology, NIAMS and","institution_ids":["https://openalex.org/I4210087962"]}]},{"author_position":"middle","author":{"id":null,"display_name":"X. Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210087962","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"X. Wu","raw_affiliation_strings":["Flow Cytometry Section, Office of Science and Technology, NIAMS and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Flow Cytometry Section, Office of Science and Technology, NIAMS and","institution_ids":["https://openalex.org/I4210087962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056798668","display_name":"Amrie C. Grammer","orcid":"https://orcid.org/0000-0001-7142-2284","observed_orcids":["https://orcid.org/0000-0001-7142-2284"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210087962","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. Grammer","raw_affiliation_strings":["Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043874873","display_name":"Peter E. Lipsky","orcid":"https://orcid.org/0000-0002-9287-1676","observed_orcids":["https://orcid.org/0000-0002-9287-1676"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210087962","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. Lipsky","raw_affiliation_strings":["Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023528108","display_name":"Gordon L. Hager","orcid":"https://orcid.org/0000-0002-9300-5331","observed_orcids":["https://orcid.org/0000-0002-9300-5331"]},"institutions":[{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G. L. Hager","raw_affiliation_strings":["CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,","institution_ids":["https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056179411","display_name":"James G. McNally","orcid":"https://orcid.org/0000-0002-9032-1988","observed_orcids":["https://orcid.org/0000-0002-9032-1988"]},"institutions":[{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"id":"https://openalex.org/I4210149717","display_name":"Center for Cancer Research","ror":"https://ror.org/05bjen692","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"J. G. McNally","raw_affiliation_strings":["CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI,","institution_ids":["https://openalex.org/I4210140884","https://openalex.org/I4210149717"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5056179411"],"corresponding_institution_ids":["https://openalex.org/I4210140884","https://openalex.org/I4210149717"],"apc_list":{"value":4760,"currency":"USD","value_usd":4760},"apc_paid":null,"fwci":15.9274,"has_fulltext":false,"cited_by_count":308,"citation_normalized_percentile":{"value":0.99475655,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":100},"biblio":{"volume":"209","issue":"1","first_page":"56","last_page":"70"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11129","display_name":"Photochemistry and Electron Transfer Studies","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11177","display_name":"Spectroscopy and Quantum Chemical Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescence-resonance-energy-transfer","display_name":"fluorescence resonance energy transfer","score":0.9990000128746033},{"id":"https://openalex.org/keywords/acceptor-photobleaching","display_name":"acceptor photobleaching","score":0.9120000004768372},{"id":"https://openalex.org/keywords/cyan-fluorescent-protein","display_name":"cyan fluorescent protein","score":0.9570000171661377},{"id":"https://openalex.org/keywords/yellow-fluorescent-protein","display_name":"YFP","score":1.0},{"id":"https://openalex.org/keywords/traf-proteins","display_name":"TRAF proteins","score":0.6499999761581421},{"id":"https://openalex.org/keywords/protein-trimerization","display_name":"protein trimerization","score":0.6430000066757202},{"id":"https://openalex.org/keywords/negative-control","display_name":"negative control","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.955258846282959},{"id":"https://openalex.org/C31332276","wikidata":"https://www.wikidata.org/wiki/Q899750","display_name":"Photobleaching","level":3,"score":0.8972345590591431},{"id":"https://openalex.org/C2778067846","wikidata":"https://www.wikidata.org/wiki/Q575416","display_name":"Yellow fluorescent protein","level":3,"score":0.8164705634117126},{"id":"https://openalex.org/C136009344","wikidata":"https://www.wikidata.org/wiki/Q336201","display_name":"Confocal","level":2,"score":0.6440029740333557},{"id":"https://openalex.org/C67649825","wikidata":"https://www.wikidata.org/wiki/Q196538","display_name":"Microscope","level":2,"score":0.6072760820388794},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5881704092025757},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5515076518058777},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.5361011028289795},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5241743922233582},{"id":"https://openalex.org/C26659971","wikidata":"https://www.wikidata.org/wiki/Q729923","display_name":"Fluorescence recovery after photobleaching","level":3,"score":0.47823113203048706},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.475591778755188},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4207687973976135},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.41901636123657227},{"id":"https://openalex.org/C2779892579","wikidata":"https://www.wikidata.org/wiki/Q912138","display_name":"Acceptor","level":2,"score":0.4149627685546875},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.2324409782886505},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12081772089004517},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.08100888133049011},{"id":"https://openalex.org/C26873012","wikidata":"https://www.wikidata.org/wiki/Q214781","display_name":"Condensed matter physics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004735","descriptor_name":"Energy Transfer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D038761","descriptor_name":"Photobleaching","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-2818.2003.01100.x","doi":"https://doi.org/10.1046/j.1365-2818.2003.01100.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2818.2003.01100.x","pdf_url":null,"source":{"id":"https://openalex.org/S168975578","display_name":"Journal of Microscopy","issn_l":"0022-2720","issn":["0022-2720","1365-2818"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Microscopy","raw_type":"journal-article"},{"id":"pmid:12535185","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12535185","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microscopy","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W171251970","https://openalex.org/W572750220","https://openalex.org/W1481249341","https://openalex.org/W1481483713","https://openalex.org/W1542263802","https://openalex.org/W1559720000","https://openalex.org/W1573145410","https://openalex.org/W1965797967","https://openalex.org/W1973680986","https://openalex.org/W1991934631","https://openalex.org/W1997681070","https://openalex.org/W1997731814","https://openalex.org/W2000151769","https://openalex.org/W2001528961","https://openalex.org/W2009654376","https://openalex.org/W2011534687","https://openalex.org/W2021628061","https://openalex.org/W2022054103","https://openalex.org/W2037427470","https://openalex.org/W2055864348","https://openalex.org/W2056625364","https://openalex.org/W2061104342","https://openalex.org/W2068924803","https://openalex.org/W2073009787","https://openalex.org/W2077835601","https://openalex.org/W2082809022","https://openalex.org/W2094328965","https://openalex.org/W2101785963","https://openalex.org/W2107245918","https://openalex.org/W2125551363","https://openalex.org/W2127068865","https://openalex.org/W2136259733","https://openalex.org/W2154298653","https://openalex.org/W2161996750","https://openalex.org/W2165685835","https://openalex.org/W2410633428","https://openalex.org/W2435834244"],"related_works":["https://openalex.org/W2120961682","https://openalex.org/W2013484632","https://openalex.org/W1599035594","https://openalex.org/W3142140511","https://openalex.org/W1993389835","https://openalex.org/W2135157957","https://openalex.org/W2006003203","https://openalex.org/W7262946","https://openalex.org/W1997857150","https://openalex.org/W7098081786"],"abstract_inverted_index":{"One":[0],"manifestation":[1],"of":[2,41,96,150,160,171,181],"fluorescence":[3,13],"resonance":[4],"energy":[5],"transfer":[6],"(FRET)":[7],"is":[8],"an":[9,154],"increase":[10],"in":[11,110,141,168],"donor":[12,167],"after":[14],"photobleaching":[15],"the":[16,39,51,57,88,101,161,169,172],"acceptor.":[17],"Published":[18],"acceptor-photobleaching":[19],"methods":[20],"for":[21,64,93,197,225],"FRET":[22,67,109,142,191,211,227],"have":[23],"mainly":[24],"used":[25],"wide-field":[26],"microscopy.":[27],"A":[28],"laser":[29,75],"scanning":[30],"confocal":[31,82,219],"microscope":[32],"enables":[33],"faster":[34],"and":[35,46,55,98,103,114,127,163,221],"targeted":[36],"bleaching":[37],"within":[38],"field":[40],"view,":[42],"thereby":[43],"improving":[44],"speed":[45],"accuracy.":[47],"Here":[48],"we":[49,85,91,130,193,204],"demonstrate":[50,131,222],"approach":[52,209],"with":[53,72,100,166],"CFP":[54,97],"YFP,":[56],"most":[58,217],"versatile":[59],"fluorescent":[60],"markers":[61],"now":[62],"available":[63,77],"FRET.":[65,187],"CFP/YFP":[66,112,230],"imaging":[68,95],"has":[69],"been":[70],"accomplished":[71],"a":[73,111,195,207],"single":[74],"(argon)":[76],"on":[78],"virtually":[79],"all":[80],"laser-scanning":[81,218],"microscopes.":[83],"Accordingly,":[84],"also":[86,115],"describe":[87,194],"conditions":[89],"that":[90,122,132,178,212],"developed":[92],"dual":[94],"YFP":[99],"458":[102],"514":[104],"argon":[105],"lines.":[106],"We":[107,146,176],"detect":[108],"fusion":[113],"between":[116,228],"signalling":[117],"molecules":[118],"(TNF-Receptor-Associated-Factors":[119],"or":[120],"TRAFs)":[121],"are":[123,135],"known":[124],"to":[125,137,210,216],"homo-":[126],"heterotrimerize.":[128],"Importantly,":[129],"appropriate":[133],"controls":[134],"essential":[136],"avoid":[138],"false":[139,186,190],"positives":[140],"by":[143],"acceptor":[144,173],"photobleaching.":[145],"use":[147],"two":[148],"types":[149,180],"negative":[151,156,182],"control:":[152],"(a)":[153],"internal":[155],"control":[157,183],"(non-bleached":[158],"areas":[159],"cell)":[162],"(b)":[164],"cells":[165],"absence":[170],"(CFP":[174],"only).":[175],"find":[177],"both":[179],"can":[184],"yield":[185],"Given":[188],"this":[189],"background,":[192],"method":[196],"distinguishing":[198],"true":[199],"positive":[200],"signals.":[201],"In":[202],"summary,":[203],"extensively":[205],"characterize":[206],"simple":[208],"should":[213],"be":[214],"adaptable":[215],"microscopes,":[220],"its":[223],"feasibility":[224],"detecting":[226],"several":[229],"partners.":[231]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":8},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":17},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":9},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":17}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}