{"id":"https://openalex.org/W1985153444","doi":"https://doi.org/10.1080/10942910701867764","title":"Alpha-Amylase from Persimmon Honey: Purification and Characterization","display_name":"Alpha-Amylase from Persimmon Honey: Purification and Characterization","publication_year":2009,"publication_date":"2009-05-06","ids":{"openalex":"https://openalex.org/W1985153444","doi":"https://doi.org/10.1080/10942910701867764","mag":"1985153444"},"language":"en","primary_location":{"id":"doi:10.1080/10942910701867764","doi":"https://doi.org/10.1080/10942910701867764","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10942910701867764","pdf_url":null,"source":{"id":"https://openalex.org/S80488789","display_name":"International Journal of Food Properties","issn_l":"1094-2912","issn":["1094-2912","1532-2386"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310315681","host_organization_name":"Marcel Dekker","host_organization_lineage":["https://openalex.org/P4310315681"],"host_organization_lineage_names":["Marcel Dekker"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Food Properties","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048345271","display_name":"Takeshi Nagai","orcid":"https://orcid.org/0000-0002-2081-8497","observed_orcids":["https://orcid.org/0000-0002-2081-8497"]},"institutions":[{"id":"https://openalex.org/I127317711","display_name":"Tokyo University of Agriculture","ror":"https://ror.org/05crbcr45","country_code":"JP","type":"education","lineage":["https://openalex.org/I127317711"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takeshi Nagai","raw_affiliation_strings":["Department of Food Science and Technology , Tokyo University of Agriculture , Hokkaido, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology , Tokyo University of Agriculture , Hokkaido, Japan","institution_ids":["https://openalex.org/I127317711"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024244470","display_name":"Reiji Inoue","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Reiji Inoue","raw_affiliation_strings":["Inoue Yohojo Bee Farm Inc. , Hyogo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Inoue Yohojo Bee Farm Inc. , Hyogo, Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071996788","display_name":"Nobutaka Suzuki","orcid":"https://orcid.org/0000-0002-7542-3097","observed_orcids":["https://orcid.org/0000-0002-7542-3097"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nobutaka Suzuki","raw_affiliation_strings":["Nagoya Research Institute , Toyoake, Aichi, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nagoya Research Institute , Toyoake, Aichi, Japan","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062064403","display_name":"Toshio Nagashima","orcid":"https://orcid.org/0000-0003-0832-2066","observed_orcids":["https://orcid.org/0000-0003-0832-2066"]},"institutions":[{"id":"https://openalex.org/I127317711","display_name":"Tokyo University of Agriculture","ror":"https://ror.org/05crbcr45","country_code":"JP","type":"education","lineage":["https://openalex.org/I127317711"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toshio Nagashima","raw_affiliation_strings":["Department of Food Science and Technology , Tokyo University of Agriculture , Hokkaido, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology , Tokyo University of Agriculture , Hokkaido, Japan","institution_ids":["https://openalex.org/I127317711"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1880,"currency":"GBP","value_usd":2306},"apc_paid":null,"fwci":0.5436,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.6278872,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"12","issue":"3","first_page":"512","last_page":"521"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8070999979972839,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8070999979972839,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11386","display_name":"Bee Products Chemical Analysis","score":0.16269999742507935,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.012299999594688416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-amylase","display_name":"alpha-amylase","score":0.9490000009536743},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.972000002861023},{"id":"https://openalex.org/keywords/enzyme-characterization","display_name":"enzyme characterization","score":0.890999972820282},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.47600001096725464},{"id":"https://openalex.org/keywords/metal-ion-effects-on-enzyme-activity","display_name":"metal ion effects on enzyme activity","score":0.621999979019165},{"id":"https://openalex.org/keywords/enzyme-inactivation","display_name":"enzyme inactivation","score":0.4830000102519989},{"id":"https://openalex.org/keywords/monomeric-enzyme","display_name":"monomeric enzyme","score":0.5920000076293945}],"concepts":[{"id":"https://openalex.org/C173959671","wikidata":"https://www.wikidata.org/wiki/Q106951023","display_name":"Alpha-amylase","level":4,"score":0.7344840168952942},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.6445409059524536},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5009377002716064},{"id":"https://openalex.org/C64943373","wikidata":"https://www.wikidata.org/wiki/Q2651003","display_name":"Alpha (finance)","level":4,"score":0.4553968012332916},{"id":"https://openalex.org/C170835558","wikidata":"https://www.wikidata.org/wiki/Q17153","display_name":"Amylase","level":3,"score":0.4072730839252472},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2225380539894104},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19623002409934998},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.1653957962989807},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.06657800078392029},{"id":"https://openalex.org/C49453240","wikidata":"https://www.wikidata.org/wiki/Q1592163","display_name":"Construct validity","level":3,"score":0.0},{"id":"https://openalex.org/C171606756","wikidata":"https://www.wikidata.org/wiki/Q506132","display_name":"Psychometrics","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1080/10942910701867764","doi":"https://doi.org/10.1080/10942910701867764","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10942910701867764","pdf_url":null,"source":{"id":"https://openalex.org/S80488789","display_name":"International Journal of Food Properties","issn_l":"1094-2912","issn":["1094-2912","1532-2386"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310315681","host_organization_name":"Marcel Dekker","host_organization_lineage":["https://openalex.org/P4310315681"],"host_organization_lineage_names":["Marcel Dekker"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Food Properties","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1565108003","https://openalex.org/W1601182769","https://openalex.org/W1775749144","https://openalex.org/W1966725477","https://openalex.org/W1972755830","https://openalex.org/W1978901456","https://openalex.org/W1993710298","https://openalex.org/W2002074043","https://openalex.org/W2004153309","https://openalex.org/W2035883873","https://openalex.org/W2037999808","https://openalex.org/W2042771917","https://openalex.org/W2050850244","https://openalex.org/W2065840967","https://openalex.org/W2100837269","https://openalex.org/W2121117307","https://openalex.org/W2124950643"],"related_works":["https://openalex.org/W2036248642","https://openalex.org/W1963771413","https://openalex.org/W1978901456","https://openalex.org/W1966725477","https://openalex.org/W25340106","https://openalex.org/W166369897","https://openalex.org/W2571555945","https://openalex.org/W2341259012","https://openalex.org/W2074433659","https://openalex.org/W1995244279"],"abstract_inverted_index":{"The":[0,59,100,109],"\u03b1-amylase":[1],"was":[2,25,45,53,61,66,103],"extracted":[3],"from":[4,136],"pure":[5],"persimmon":[6],"honey":[7],"and":[8,15,37,55,88,97,124],"purified":[9,23,43,101],"by":[10,32,82,92,106],"DEAE-Toyopearl":[11],"650M,":[12,14],"CM-Toyopearl":[13],"Toyopearl":[16,33],"HW-55F":[17,34],"column":[18],"chromatographies.":[19],"Molecular":[20],"weight":[21],"of":[22,50,104,112,114,122,128],"enzyme":[24,44,52,60,80,102],"estimated":[26],"to":[27,133],"be":[28],"about":[29],"58":[30],"kDa":[31],"gel":[35],"chromatography":[36],"SDS-PAGE,":[38],"respectively":[39],"suggested":[40],"that":[41],"the":[42,51,115,134],"a":[46],"monomer.":[47],"Optimum":[48],"pH":[49,65],"6.0\u22127.0":[54],"optimum":[56],"temperature":[57],"40\u00b0C.":[58,78],"extremely":[62],"inactivated":[63],"at":[64,77],"higher":[67],"than":[68,72],"7.0":[69],"or":[70],"lower":[71],"5.0.":[73],"Heat":[74],"inactivation":[75],"occurred":[76],"This":[79],"activated":[81],"Ca":[83],"2+":[84],",":[85],"Mn2+,":[86],"PCMB,":[87],"DTNB,":[89],"but":[90],"inhibited":[91],"Ba2+,":[93],"Fe3+,":[94],"Hg2+,":[95],"Mg2+,":[96],"iodoacetic":[98],"acid.":[99],"\u03b1\u2009-type":[105],"TLC":[107],"analysis.":[108],"relative":[110],"rate":[111],"hydrolysis":[113],"polymeric":[116],"substance":[117],"decreased":[118],"with":[119,125],"decreasing":[120],"percentage":[121,127],"\u03b1\u2009-1,4-linkages":[123],"increasing":[126],"\u03b1\u2009-1,6-linkages":[129],"in":[130],"substrate":[131],"similar":[132],"results":[135],"commercially":[137],"available":[138],"honey.":[139]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2016-06-24T00:00:00"}