{"id":"https://openalex.org/W1983995167","doi":"https://doi.org/10.1094/mpmi.2004.17.2.162","title":"Identification and Characterization of a Well-Defined Series of Coronatine Biosynthetic Mutants of Pseudomonas syringae pv. tomato DC3000","display_name":"Identification and Characterization of a Well-Defined Series of Coronatine Biosynthetic Mutants of Pseudomonas syringae pv. tomato DC3000","publication_year":2004,"publication_date":"2004-02-01","ids":{"openalex":"https://openalex.org/W1983995167","doi":"https://doi.org/10.1094/mpmi.2004.17.2.162","mag":"1983995167","pmid":"https://pubmed.ncbi.nlm.nih.gov/14964530"},"language":"en","primary_location":{"id":"doi:10.1094/mpmi.2004.17.2.162","doi":"https://doi.org/10.1094/mpmi.2004.17.2.162","is_oa":false,"landing_page_url":"https://doi.org/10.1094/mpmi.2004.17.2.162","pdf_url":null,"source":{"id":"https://openalex.org/S88768767","display_name":"Molecular Plant-Microbe Interactions","issn_l":"0894-0282","issn":["0894-0282","1943-7706"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Plant-Microbe Interactions\u00ae","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110683300","display_name":"David M. Brooks","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David M. Brooks","raw_affiliation_strings":["Department of Biology, Washington University, St. Louis, MO 63130, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Washington University, St. Louis, MO 63130, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027011804","display_name":"Gustavo Hern\u00e1ndez-Guzm\u00e1n","orcid":"https://orcid.org/0000-0002-3686-8122","observed_orcids":["https://orcid.org/0000-0002-3686-8122"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gustavo Hern\u00e1ndez-Guzm\u00e1n","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057327107","display_name":"Andrew P. Kloek","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Andrew P. Kloek","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075329903","display_name":"Francisco J. Alarc\u00f3n-Chaidez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Francisco Alarc\u00f3n-Chaidez","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110385145","display_name":"Aswathy Sreedharan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Aswathy Sreedharan","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079491947","display_name":"Vidhya Rangaswamy","orcid":"https://orcid.org/0000-0002-7543-6998","observed_orcids":["https://orcid.org/0000-0002-7543-6998"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Vidhya Rangaswamy","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090468737","display_name":"Alejandro Pe\u00f1aloza\u2010V\u00e1zquez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alejandro Pe\u00f1aloza-V\u00e1zquez","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110203616","display_name":"Carol L. Bender","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Carol L. Bender","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039743643","display_name":"Barbara N. Kunkel","orcid":"https://orcid.org/0000-0003-0309-2644","observed_orcids":["https://orcid.org/0000-0003-0309-2644"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Barbara N. Kunkel","raw_affiliation_strings":["Corresponding author: B. N. Kunkel, Campus Box 1137, 1 Brookings Dr.; E-mail: \r\n                    E-mail Address:\r\n                    [email\u00a0protected]; Telephone: 314.935.7284; Fax: 314.935.4432"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Corresponding author: B. N. Kunkel, Campus Box 1137, 1 Brookings Dr.; E-mail: \r\n                    E-mail Address:\r\n                    [email\u00a0protected]; Telephone: 314.935.7284; Fax: 314.935.4432","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":11.9626,"has_fulltext":false,"cited_by_count":211,"citation_normalized_percentile":{"value":0.98585564,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"17","issue":"2","first_page":"162","last_page":"174"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.008999999612569809,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/coronatine","display_name":"coronatine","score":0.7910000085830688},{"id":"https://openalex.org/keywords/pseudomonas-syringae-pv-tomato-dc3000","display_name":"Pseudomonas syringae pv. tomato DC3000","score":0.9919999837875366},{"id":"https://openalex.org/keywords/virulence-loci","display_name":"virulence loci","score":0.7080000042915344},{"id":"https://openalex.org/keywords/tn5-mutants","display_name":"Tn5 transposon mutants","score":0.777999997138977},{"id":"https://openalex.org/keywords/coronafacic-acid","display_name":"coronafacic acid","score":0.8600000143051147},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8870000243186951},{"id":"https://openalex.org/keywords/plant-toxins","display_name":"plant toxins","score":0.7910000085830688}],"concepts":[{"id":"https://openalex.org/C2780381237","wikidata":"https://www.wikidata.org/wiki/Q311202","display_name":"Pseudomonas syringae","level":3,"score":0.9852446913719177},{"id":"https://openalex.org/C2780393528","wikidata":"https://www.wikidata.org/wiki/Q5172198","display_name":"Coronatine","level":5,"score":0.9329604506492615},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.8929635286331177},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.8510754704475403},{"id":"https://openalex.org/C2775856171","wikidata":"https://www.wikidata.org/wiki/Q1872710","display_name":"Phytotoxin","level":3,"score":0.7957397699356079},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7412410974502563},{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.6664971709251404},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5366325974464417},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5356106162071228},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.40825143456459045},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3481980562210083},{"id":"https://openalex.org/C2777367657","wikidata":"https://www.wikidata.org/wiki/Q184651","display_name":"Toxin","level":2,"score":0.15873783826828003}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003360","descriptor_name":"Cosmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007192","descriptor_name":"Indenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010935","descriptor_name":"Plant Diseases","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D014774","descriptor_name":"Virulence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D018551","descriptor_name":"Solanum lycopersicum","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D044224","descriptor_name":"Pseudomonas syringae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D044224","descriptor_name":"Pseudomonas syringae","qualifier_ui":"Q000472","qualifier_name":"pathogenicity","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1094/mpmi.2004.17.2.162","doi":"https://doi.org/10.1094/mpmi.2004.17.2.162","is_oa":false,"landing_page_url":"https://doi.org/10.1094/mpmi.2004.17.2.162","pdf_url":null,"source":{"id":"https://openalex.org/S88768767","display_name":"Molecular Plant-Microbe Interactions","issn_l":"0894-0282","issn":["0894-0282","1943-7706"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Plant-Microbe Interactions\u00ae","raw_type":"journal-article"},{"id":"pmid:14964530","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14964530","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular plant-microbe interactions : MPMI","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:844046106daf464f8359022a105bbb9a","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/844046106daf464f8359022a105bbb9a","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Molecular Plant-Microbe Interactions, Vol 17, Iss 2, Pp 162-174 (2004)","raw_type":"article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.8409000039100647,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320306084","display_name":"U.S. Department of Energy","ror":"https://ror.org/01bj3aw27"},{"id":"https://openalex.org/F4320311350","display_name":"Life Sciences Research Foundation","ror":"https://ror.org/0195dxj21"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":60,"referenced_works":["https://openalex.org/W184067711","https://openalex.org/W1485966967","https://openalex.org/W1501920547","https://openalex.org/W1572699787","https://openalex.org/W1573032517","https://openalex.org/W1584407486","https://openalex.org/W1675781815","https://openalex.org/W1694199808","https://openalex.org/W1763493835","https://openalex.org/W1787690277","https://openalex.org/W1882830884","https://openalex.org/W1925709767","https://openalex.org/W1972010400","https://openalex.org/W1974058207","https://openalex.org/W1975359899","https://openalex.org/W1975563531","https://openalex.org/W1989220317","https://openalex.org/W1991136617","https://openalex.org/W2011586678","https://openalex.org/W2027229485","https://openalex.org/W2041211295","https://openalex.org/W2048291392","https://openalex.org/W2056166691","https://openalex.org/W2057238625","https://openalex.org/W2068007578","https://openalex.org/W2068201284","https://openalex.org/W2076689852","https://openalex.org/W2080000878","https://openalex.org/W2083042859","https://openalex.org/W2084879740","https://openalex.org/W2087901202","https://openalex.org/W2103361794","https://openalex.org/W2113117904","https://openalex.org/W2113702411","https://openalex.org/W2114285279","https://openalex.org/W2114343191","https://openalex.org/W2115997854","https://openalex.org/W2117807796","https://openalex.org/W2124605657","https://openalex.org/W2126254780","https://openalex.org/W2126956220","https://openalex.org/W2129656757","https://openalex.org/W2132491389","https://openalex.org/W2136855264","https://openalex.org/W2137352607","https://openalex.org/W2137749186","https://openalex.org/W2148997130","https://openalex.org/W2154423647","https://openalex.org/W2157301155","https://openalex.org/W2158937196","https://openalex.org/W2167439121","https://openalex.org/W2169345640","https://openalex.org/W2170363294","https://openalex.org/W2205425490","https://openalex.org/W2324193054","https://openalex.org/W2326077057","https://openalex.org/W2333476351","https://openalex.org/W2333825832","https://openalex.org/W2334542882","https://openalex.org/W3023948558"],"related_works":["https://openalex.org/W7071159343","https://openalex.org/W2124418643","https://openalex.org/W2055855243","https://openalex.org/W2205425490","https://openalex.org/W2049809388","https://openalex.org/W2068007578","https://openalex.org/W2075801620","https://openalex.org/W4253507539","https://openalex.org/W2091390911","https://openalex.org/W1694199808"],"abstract_inverted_index":{"To":[0,142],"identify":[1],"Pseudomonas":[2],"syringae":[3,20,55],"pv.":[4,21,56],"tomato":[5,22,57],"genes":[6,47,60,110],"involved":[7],"in":[8,45,67,90,105,125,152,220],"pathogenesis,":[9],"we":[10,154],"carried":[11],"out":[12],"a":[13],"screen":[14],"for":[15,48,75,174],"Tn5":[16,103],"mutants":[17,31,42,121,187,213],"of":[18,78,147,185,196,218],"P.":[19,54],"DC3000":[23,58],"with":[24],"reduced":[25,183],"virulence":[26,37,165,184],"on":[27,138,167,188],"Arabidopsis":[28],"thaliana.":[29],"Several":[30],"defining":[32],"both":[33],"known":[34],"and":[35,64,97,108,113,133,150,156,209],"novel":[36],"loci":[38],"were":[39,122],"identified.":[40],"Six":[41],"contained":[43],"insertions":[44,104],"biosynthetic":[46,120],"the":[49,76,86,106,144,175,194,197,203,216],"phytotoxin":[50],"coronatine":[51],"(COR).":[52],"The":[53],"COR":[59,91,119,151,199,212,219],"are":[61,65],"chromosomally":[62],"encoded":[63],"arranged":[66],"two":[68,87],"separate":[69],"clusters,":[70],"which":[71],"encode":[72],"enzymes":[73],"responsible":[74],"synthesis":[77],"coronafacic":[79],"acid":[80,84],"(CFA)":[81],"or":[82,177],"coronamic":[83],"(CMA),":[85],"defined":[88,211],"intermediates":[89],"biosynthesis.":[92],"High-performance":[93],"liquid":[94],"chromatography":[95],"fractionation":[96],"exogenous":[98],"feeding":[99],"studies":[100],"confirmed":[101],"that":[102,182],"cfa":[107],"cma":[109],"disrupt":[111],"CFA":[112],"CMA":[114],"biosynthesis,":[115],"respectively.":[116],"All":[117],"six":[118],"significantly":[123],"impaired":[124],"their":[126],"ability":[127],"to":[128,130,134,171,206,214],"multiply":[129],"high":[131],"levels":[132],"elicit":[135],"disease":[136],"symptoms":[137],"A.":[139,168,189],"thaliana":[140,190],"plants.":[141],"assess":[143],"relative":[145],"contributions":[146],"CFA,":[148],"CMA,":[149],"virulence,":[153],"constructed":[155],"characterized":[157],"cfa6":[158,178],"cmaA":[159,176],"double":[160],"mutant":[161],"strains.":[162],"These":[163],"exhibited":[164],"phenotypes":[166],"thalliana":[169],"identical":[170],"those":[172],"observed":[173],"single":[179],"mutants,":[180],"suggesting":[181],"these":[186],"is":[191,202],"caused":[192],"by":[193],"absence":[195],"intact":[198],"toxin.":[200],"This":[201],"first":[204],"study":[205],"use":[207],"biochemically":[208],"genetically":[210],"address":[215],"role":[217],"pathogenesis.":[221]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":11},{"year":2021,"cited_by_count":12},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":11},{"year":2018,"cited_by_count":14},{"year":2017,"cited_by_count":11},{"year":2016,"cited_by_count":14},{"year":2015,"cited_by_count":12},{"year":2014,"cited_by_count":12},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}