{"id":"https://openalex.org/W1983759759","doi":"https://doi.org/10.1073/pnas.72.8.3073","title":"Acetylation of prostaglandin synthase by aspirin.","display_name":"Acetylation of prostaglandin synthase by aspirin.","publication_year":1975,"publication_date":"1975-08-01","ids":{"openalex":"https://openalex.org/W1983759759","doi":"https://doi.org/10.1073/pnas.72.8.3073","mag":"1983759759","pmid":"https://pubmed.ncbi.nlm.nih.gov/810797","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/432922"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.72.8.3073","doi":"https://doi.org/10.1073/pnas.72.8.3073","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.72.8.3073","pdf_url":"https://pnas.org/doi/full/10.1073/pnas.72.8.3073","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W1983759759"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://pnas.org/doi/full/10.1073/pnas.72.8.3073","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070565589","display_name":"G J Roth","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G J Roth","raw_affiliation_strings":["Divisions of Hematology and Oncology, Departments of Internal Medicine and Biochemistry, Washington University School of Medicine, St. Louis, Mo. 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Divisions of Hematology and Oncology, Departments of Internal Medicine and Biochemistry, Washington University School of Medicine, St. Louis, Mo. 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015092654","display_name":"Nancy Stanford","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N Stanford","raw_affiliation_strings":["Divisions of Hematology and Oncology, Departments of Internal Medicine and Biochemistry, Washington University School of Medicine, St. Louis, Mo. 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Divisions of Hematology and Oncology, Departments of Internal Medicine and Biochemistry, Washington University School of Medicine, St. Louis, Mo. 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113994527","display_name":"Philip W. Majerus","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P W Majerus","raw_affiliation_strings":["Divisions of Hematology and Oncology, Departments of Internal Medicine and Biochemistry, Washington University School of Medicine, St. Louis, Mo. 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Divisions of Hematology and Oncology, Departments of Internal Medicine and Biochemistry, Washington University School of Medicine, St. Louis, Mo. 63110","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204465549"],"apc_list":null,"apc_paid":null,"fwci":21.1912,"has_fulltext":true,"cited_by_count":1023,"citation_normalized_percentile":{"value":0.99672619,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"72","issue":"8","first_page":"3073","last_page":"3076"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12910","display_name":"Eicosanoids and Hypertension Pharmacology","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspirin","display_name":"aspirin","score":0.9879999756813049},{"id":"https://openalex.org/keywords/acetylation","display_name":"acetylation","score":0.8109999895095825},{"id":"https://openalex.org/keywords/cyclooxygenase","display_name":"cyclooxygenase","score":0.9010000228881836},{"id":"https://openalex.org/keywords/prostaglandin-synthase","display_name":"prostaglandin synthetase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/arachidonic-acid","display_name":"arachidonic acid","score":0.8740000128746033},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.5630000233650208},{"id":"https://openalex.org/keywords/indomethacin","display_name":"indomethacin","score":0.8960000276565552},{"id":"https://openalex.org/keywords/anti-inflammatory-activity","display_name":"anti-inflammatory activity","score":0.7929999828338623},{"id":"https://openalex.org/keywords/michaelis-constant","display_name":"Michaelis constant","score":0.6700000166893005}],"concepts":[{"id":"https://openalex.org/C119157956","wikidata":"https://www.wikidata.org/wiki/Q796604","display_name":"Acetylation","level":3,"score":0.6937894821166992},{"id":"https://openalex.org/C2778078955","wikidata":"https://www.wikidata.org/wiki/Q407699","display_name":"Arachidonic acid","level":3,"score":0.6867314577102661},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6708561778068542},{"id":"https://openalex.org/C2779134981","wikidata":"https://www.wikidata.org/wiki/Q414547","display_name":"Sodium salicylate","level":2,"score":0.661957859992981},{"id":"https://openalex.org/C2777628954","wikidata":"https://www.wikidata.org/wiki/Q18216","display_name":"Aspirin","level":2,"score":0.6520185470581055},{"id":"https://openalex.org/C2780664492","wikidata":"https://www.wikidata.org/wiki/Q209717","display_name":"Prostaglandin","level":2,"score":0.6073600649833679},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6071489453315735},{"id":"https://openalex.org/C2778221352","wikidata":"https://www.wikidata.org/wiki/Q193572","display_name":"Salicylic acid","level":2,"score":0.59163498878479},{"id":"https://openalex.org/C2779689624","wikidata":"https://www.wikidata.org/wiki/Q410251","display_name":"Cyclooxygenase","level":3,"score":0.5204622745513916},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.46115991473197937},{"id":"https://openalex.org/C87644729","wikidata":"https://www.wikidata.org/wiki/Q547502","display_name":"Microsome","level":3,"score":0.4597729444503784},{"id":"https://openalex.org/C112243037","wikidata":"https://www.wikidata.org/wiki/Q22327117","display_name":"ATP synthase","level":3,"score":0.42049872875213623},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.050085991621017456},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000107","descriptor_name":"Acetylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001095","descriptor_name":"Arachidonic Acids","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D001241","descriptor_name":"Aspirin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001792","descriptor_name":"Blood Platelets","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002621","descriptor_name":"Chemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005230","descriptor_name":"Fatty Acids, Nonesterified","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007213","descriptor_name":"Indomethacin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008861","descriptor_name":"Microsomes","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011451","descriptor_name":"Prostaglandin-Endoperoxide Synthases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012669","descriptor_name":"Seminal Vesicles","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1073/pnas.72.8.3073","doi":"https://doi.org/10.1073/pnas.72.8.3073","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.72.8.3073","pdf_url":"https://pnas.org/doi/full/10.1073/pnas.72.8.3073","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:66488","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC432922","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:810797","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/810797","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:432922","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC432922/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"mag:2186181351","doi":null,"is_oa":false,"landing_page_url":"https://www.pnas.org/content/72/8/3073.full.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.1073/pnas.72.8.3073","doi":"https://doi.org/10.1073/pnas.72.8.3073","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.72.8.3073","pdf_url":"https://pnas.org/doi/full/10.1073/pnas.72.8.3073","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1983759759.pdf","grobid_xml":"https://content.openalex.org/works/W1983759759.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W1492100345","https://openalex.org/W1567233573","https://openalex.org/W1975021398","https://openalex.org/W1975989619","https://openalex.org/W1981770747","https://openalex.org/W1994039744","https://openalex.org/W2008574207","https://openalex.org/W2010694924","https://openalex.org/W2010784300","https://openalex.org/W2049466131","https://openalex.org/W2050568232","https://openalex.org/W2054021443","https://openalex.org/W2056782934","https://openalex.org/W2071112954","https://openalex.org/W2077459776","https://openalex.org/W2080994996","https://openalex.org/W2088748703","https://openalex.org/W2120299428","https://openalex.org/W2423381978","https://openalex.org/W2460884651","https://openalex.org/W6603117841","https://openalex.org/W6833232041"],"related_works":["https://openalex.org/W1997647638","https://openalex.org/W1572379239","https://openalex.org/W1976011608","https://openalex.org/W2140482107","https://openalex.org/W1660326086","https://openalex.org/W2085965632","https://openalex.org/W2025237854","https://openalex.org/W2121073066","https://openalex.org/W1577034263","https://openalex.org/W2011946737"],"abstract_inverted_index":{"When":[0],"microsomes":[1],"of":[2,20,38,52,75,93,98,134,176],"sheep":[3],"or":[4],"bovine":[5],"seminal":[6],"vesicles":[7],"are":[8,101],"incubated":[9],"with":[10,61,130],"[acetyl-3H]aspirin":[11],"(acetyl":[12],"salicylic":[13],"acid),":[14],"200":[15],"Ci/mol,":[16],"we":[17],"observe":[18],"acetylation":[19,58,100,118,149],"a":[21,35,43,138],"single":[22],"protein,":[23],"as":[24,71,137,156,165],"measured":[25],"by":[26,84,119],"sodium":[27],"dodecyl":[28],"sulfate/polyacrylamide":[29],"gel":[30],"electrophoresis.":[31],"The":[32,56,159],"protein":[33,46,117],"has":[34],"molecular":[36],"weight":[37],"85,000":[39],"and":[40,66,96,145,183],"corresponds":[41],"to":[42,153],"similar":[44],"acetylated":[45],"found":[47],"in":[48,151],"the":[49,62,68,73,85,110,131,148,171],"particulate":[50],"fraction":[51],"aspirin-treated":[53],"human":[54],"platelets.":[55],"aspirin-mediated":[57],"reaction":[59,150],"proceeds":[60],"same":[63,69],"time":[64],"course":[65],"at":[67,106,121,125],"concentration":[70],"does":[72],"inhibition":[74,92,124],"prostaglandin":[76,94],"synthase":[77,95],"(cyclo-oxygenase)":[78],"(EC":[79],"1.14.99.1;":[80],"8,11,14-eicosatrienoate,":[81],"hydrogen-donor:oxygen":[82],"oxidoreductase)":[83],"drug.":[86],"At":[87],"100":[88],"muM":[89],"aspirin,":[90],"50%":[91,97],"maximal":[99],"observed":[102],"after":[103],"15":[104],"min":[105],"37":[107],"degrees.":[108],"Furthermore,":[109],"substrate":[111,139],"for":[112,140,170,180],"cyclo-oxygenase,":[113],"arachidonic":[114,135],"acid,":[115],"inhibits":[116],"aspirin":[120,163,177],"concentrations":[122],"(50%":[123],"10-30":[126],"muM)":[127],"which":[128],"correlate":[129],"Michaelis":[132],"constant":[133],"acid":[136,143],"cyclooxygenase.":[141],"Arachidonic":[142],"analogues":[144],"indomethacin":[146],"inhibit":[147],"proportion":[152],"their":[154],"effectiveness":[155],"cyclo-oxygenase":[157],"inhibitors.":[158],"results":[160],"suggest":[161],"that":[162],"acts":[164],"an":[166],"active-site":[167],"acetylating":[168],"agent":[169],"enzyme":[172],"cyclo-oxygenase.":[173],"This":[174],"action":[175],"may":[178],"account":[179],"its":[181],"anti-inflammatory":[182],"anti-platelet":[184],"action.":[185]},"counts_by_year":[{"year":2026,"cited_by_count":12},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":20},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":18},{"year":2021,"cited_by_count":11},{"year":2020,"cited_by_count":12},{"year":2019,"cited_by_count":18},{"year":2018,"cited_by_count":18},{"year":2017,"cited_by_count":21},{"year":2016,"cited_by_count":25},{"year":2015,"cited_by_count":28},{"year":2014,"cited_by_count":24},{"year":2013,"cited_by_count":19},{"year":2012,"cited_by_count":29}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}