{"id":"https://openalex.org/W1983443074","doi":"https://doi.org/10.1046/j.1365-3040.2000.00526.x","title":"Molecular cloning of yieldins regulating the yield threshold of cowpea cell walls: cDNA cloning and characterization of recombinant yieldin","display_name":"Molecular cloning of yieldins regulating the yield threshold of cowpea cell walls: cDNA cloning and characterization of recombinant yieldin","publication_year":2000,"publication_date":"2000-02-01","ids":{"openalex":"https://openalex.org/W1983443074","doi":"https://doi.org/10.1046/j.1365-3040.2000.00526.x","mag":"1983443074"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-3040.2000.00526.x","doi":"https://doi.org/10.1046/j.1365-3040.2000.00526.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-3040.2000.00526.x","pdf_url":null,"source":{"id":"https://openalex.org/S170036492","display_name":"Plant Cell & Environment","issn_l":"0140-7791","issn":["0140-7791","1365-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-3","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant, Cell & Environment","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5073252764","display_name":"Akane Okamoto\u2010Nakazato","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I8873088","display_name":"Showa Pharmaceutical University","ror":"https://ror.org/053e8a708","country_code":"JP","type":"education","lineage":["https://openalex.org/I8873088"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"A. Okamoto\u2010Nakazato","raw_affiliation_strings":["Biological Institute, Showa College of Pharmaceutical Sciences, 3\u20133165, Higashi-tamagawagakuen, Machida-Shi, Tokyo 194\u20138543, Japan and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Institute, Showa College of Pharmaceutical Sciences, 3\u20133165, Higashi-tamagawagakuen, Machida-Shi, Tokyo 194\u20138543, Japan and","institution_ids":["https://openalex.org/I8873088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001854753","display_name":"Koji U. Takahashi","orcid":"https://orcid.org/0000-0001-5438-7624","observed_orcids":["https://orcid.org/0000-0001-5438-7624"]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K. Takahashi","raw_affiliation_strings":["Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079329930","display_name":"Nobuo Kido","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"N. Kido","raw_affiliation_strings":["Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079984899","display_name":"K. Owaribe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K. Owaribe","raw_affiliation_strings":["Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5090435484","display_name":"Kiyoshi Katou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K. Katou","raw_affiliation_strings":["Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Unit of Biosystems, School of Informatics and Sciences and Graduate School of Human Informatics, Nagoya University, Nagoya 464\u20138601, Japan","institution_ids":["https://openalex.org/I60134161"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5073252764"],"corresponding_institution_ids":["https://openalex.org/I8873088"],"apc_list":{"value":5440,"currency":"USD","value_usd":5440},"apc_paid":null,"fwci":4.184,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.9337823,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"23","issue":"2","first_page":"155","last_page":"164"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.975600004196167,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.975600004196167,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.013799999840557575,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cowpea","display_name":"cowpea","score":0.9959999918937683},{"id":"https://openalex.org/keywords/cell-wall-mechanics","display_name":"cell wall mechanics","score":0.7039999961853027},{"id":"https://openalex.org/keywords/yield-threshold","display_name":"yield threshold","score":0.8050000071525574},{"id":"https://openalex.org/keywords/endochitinase","display_name":"endochitinase","score":0.6710000038146973},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.7300000190734863},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9549999833106995},{"id":"https://openalex.org/keywords/hypocotyl","display_name":"hypocotyl","score":0.734000027179718}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.8341953754425049},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7639876008033752},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6630165576934814},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5974962115287781},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.5665722489356995},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5297587513923645},{"id":"https://openalex.org/C2779862049","wikidata":"https://www.wikidata.org/wiki/Q132634","display_name":"Vigna","level":2,"score":0.5142847895622253},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.4755948483943939},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.4419775903224945},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.435404896736145},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.41206127405166626},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37013179063796997},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.222925066947937},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.03980681300163269}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-3040.2000.00526.x","doi":"https://doi.org/10.1046/j.1365-3040.2000.00526.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-3040.2000.00526.x","pdf_url":null,"source":{"id":"https://openalex.org/S170036492","display_name":"Plant Cell & Environment","issn_l":"0140-7791","issn":["0140-7791","1365-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-3","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant, Cell & Environment","raw_type":"journal-article"},{"id":"mag:2907341619","doi":null,"is_oa":false,"landing_page_url":"https://dissem.in/p/22120804/molecular-cloning-of-yieldins-regulating-the-yield-threshold-of-cow-pea-cell-walls-cdna-cloning-and-characterization-of-recombinant-yieldin-plant-cell-environ","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.7142999768257141,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W179735899","https://openalex.org/W1582927787","https://openalex.org/W1583419991","https://openalex.org/W1768711802","https://openalex.org/W1772341111","https://openalex.org/W1822340210","https://openalex.org/W1822715373","https://openalex.org/W1824832381","https://openalex.org/W1845185552","https://openalex.org/W1861748645","https://openalex.org/W1969224779","https://openalex.org/W1975238158","https://openalex.org/W1990513516","https://openalex.org/W1999984289","https://openalex.org/W2000475059","https://openalex.org/W2007676396","https://openalex.org/W2016940213","https://openalex.org/W2019444031","https://openalex.org/W2032118800","https://openalex.org/W2036312682","https://openalex.org/W2045497721","https://openalex.org/W2054610876","https://openalex.org/W2054848637","https://openalex.org/W2055905500","https://openalex.org/W2062989643","https://openalex.org/W2070048676","https://openalex.org/W2072144575","https://openalex.org/W2076042370","https://openalex.org/W2101746978","https://openalex.org/W2102520742","https://openalex.org/W2108859348","https://openalex.org/W2109518880","https://openalex.org/W2122347004","https://openalex.org/W2134566697","https://openalex.org/W2139053374","https://openalex.org/W2139772523","https://openalex.org/W2144156507","https://openalex.org/W2150095982","https://openalex.org/W2154286735","https://openalex.org/W2159080918","https://openalex.org/W2161729119","https://openalex.org/W2283833714","https://openalex.org/W2394122781","https://openalex.org/W2397291835","https://openalex.org/W2472660243","https://openalex.org/W2505485644"],"related_works":["https://openalex.org/W2907341619","https://openalex.org/W1986613770","https://openalex.org/W2055905500","https://openalex.org/W628692418","https://openalex.org/W396565904","https://openalex.org/W2045497721","https://openalex.org/W2108970459","https://openalex.org/W2780550145","https://openalex.org/W7052353089","https://openalex.org/W1991469111"],"abstract_inverted_index":{"ABSTRACT":[0],"cDNA":[1,47,98],"for":[2,48],"yieldin":[3,49,84,121,144,172],"of":[4,27,33,41,55,75,150,156,163,184,188],"Vigna":[5,189],"unguiculata":[6,190],"L.":[7,191],"was":[8,85,104,122,137,174],"cloned":[9],"with":[10],"reverse":[11],"transcriptase\u2010polymerase":[12],"chain":[13],"reaction":[14],"using":[15,110],"synthetic":[16],"oligonucleotides":[17],"as":[18,67],"primers.":[19],"The":[20,44,119,142],"primers":[21],"were":[22],"designed":[23],"on":[24,132],"the":[25,28,34,38,76,83,101,116,125,133,147,151,171,178,185],"basis":[26],"N\u2010terminal":[29],"amino":[30,61,65,78],"acid":[31,79],"sequence":[32,80],"yieldins":[35],"isolated":[36],"from":[37,124],"wall":[39,134],"preparation":[40],"cowpea":[42,164],"hypocotyls.":[43,165],"1\u00b72":[45],"kbp":[46],"contained":[50],"an":[51,111],"open":[52],"reading":[53],"frame":[54],"981":[56],"base":[57],"pairs,":[58],"encoding":[59],"327":[60],"acids":[62,66],"including":[63],"23":[64],"a":[68],"putative":[69],"signal":[70],"sequence.":[71],"An":[72],"homology":[73],"search":[74],"deduced":[77],"revealed":[81,169],"that":[82,170],"homologous":[86],"to":[87,106,114],"acidic":[88],"class":[89],"III":[90],"endochitinases":[91],"(EC":[92],"3\u00b72.1\u00b714)":[93],"and":[94,129,180],"concanavalin":[95],"B.":[96],"A":[97],"fragment":[99],"containing":[100],"yieldin\u2010coding":[102],"region":[103,183],"introduced":[105],"Escherichia":[107],"coli":[108,128],"cells":[109],"expression":[112],"vector":[113],"express":[115],"recombinant":[117,120,126,143],"protein.":[118],"obtained":[123],"E.":[127],"its":[130],"effect":[131],"mechanical":[135],"properties":[136],"examined":[138],"by":[139],"reconstitution":[140],"experiments.":[141],"fully":[145],"restored":[146],"acid\u2010induced":[148],"change":[149],"yield":[152],"threshold":[153],"tension":[154],"(y)":[155],"heat":[157],"denatured":[158],"glycerinated":[159],"hollow":[160],"cylinders":[161],"(GHCs)":[162],"Northern":[166],"hybridization":[167],"analysis":[168],"mRNA":[173],"expressed":[175],"mainly":[176],"in":[177],"rapid":[179],"moderate":[181],"elongation":[182],"etiolated":[186],"hypocotyl":[187]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}