{"id":"https://openalex.org/W1983207263","doi":"https://doi.org/10.1371/journal.pone.0050406","title":"One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)","display_name":"One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)","publication_year":2012,"publication_date":"2012-12-04","ids":{"openalex":"https://openalex.org/W1983207263","doi":"https://doi.org/10.1371/journal.pone.0050406","mag":"1983207263","pmid":"https://pubmed.ncbi.nlm.nih.gov/23226519","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3514308"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0050406","doi":"https://doi.org/10.1371/journal.pone.0050406","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0050406","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050406&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050406&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061923369","display_name":"Fatma Filiz Co\u015fkun-Ari","orcid":"https://orcid.org/0000-0002-8107-732X","observed_orcids":["https://orcid.org/0000-0002-8107-732X"]},"institutions":[{"id":"https://openalex.org/I1303077703","display_name":"Ministry of Health","ror":"https://ror.org/00pkvys92","country_code":"TR","type":"government","lineage":["https://openalex.org/I1303077703"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Fatma Filiz Coskun-Ari","raw_affiliation_strings":["Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey","institution_ids":["https://openalex.org/I1303077703"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029803669","display_name":"Dilek G\u00fcldemir","orcid":"https://orcid.org/0000-0002-7958-8315","observed_orcids":["https://orcid.org/0000-0002-7958-8315"]},"institutions":[{"id":"https://openalex.org/I1303077703","display_name":"Ministry of Health","ror":"https://ror.org/00pkvys92","country_code":"TR","type":"government","lineage":["https://openalex.org/I1303077703"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Dilek Guldemir","raw_affiliation_strings":["Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey","institution_ids":["https://openalex.org/I1303077703"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013592415","display_name":"R\u0131za Durmaz","orcid":"https://orcid.org/0000-0001-6561-778X","observed_orcids":["https://orcid.org/0000-0001-6561-778X"]},"institutions":[{"id":"https://openalex.org/I1303077703","display_name":"Ministry of Health","ror":"https://ror.org/00pkvys92","country_code":"TR","type":"government","lineage":["https://openalex.org/I1303077703"]},{"id":"https://openalex.org/I45642913","display_name":"K\u0131r\u0131kkale University","ror":"https://ror.org/01zhwwf82","country_code":"TR","type":"education","lineage":["https://openalex.org/I45642913"]}],"countries":["TR"],"is_corresponding":true,"raw_author_name":"Riza Durmaz","raw_affiliation_strings":["Department of Clinical Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey","Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey","institution_ids":["https://openalex.org/I45642913"]},{"raw_affiliation_string":"Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey","institution_ids":["https://openalex.org/I1303077703"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5013592415"],"corresponding_institution_ids":["https://openalex.org/I1303077703","https://openalex.org/I45642913"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":1.9096,"has_fulltext":true,"cited_by_count":25,"citation_normalized_percentile":{"value":0.844525,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"7","issue":"12","first_page":"e50406","last_page":"e50406"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10654","display_name":"Pneumonia and Respiratory Infections","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10654","display_name":"Pneumonia and Respiratory Infections","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.010200000368058681,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/streptococcus-pneumoniae","display_name":"Streptococcus pneumoniae","score":0.9990000128746033},{"id":"https://openalex.org/keywords/pneumococcal-serotyping","display_name":"pneumococcal serotyping","score":0.9079999923706055},{"id":"https://openalex.org/keywords/multiplex-pcr","display_name":"multiplex PCR","score":0.6990000009536743},{"id":"https://openalex.org/keywords/pcv13","display_name":"PCV13","score":0.8090000152587891},{"id":"https://openalex.org/keywords/13-valent-pneumococcal-conjugate-vaccine","display_name":"13-valent pneumococcal conjugate vaccine","score":0.9399999976158142},{"id":"https://openalex.org/keywords/serogroup-1","display_name":"serogroup 1","score":0.8989999890327454},{"id":"https://openalex.org/keywords/invasive-pneumococcal-disease","display_name":"invasive pneumococcal disease","score":0.8629999756813049}],"concepts":[{"id":"https://openalex.org/C2781253189","wikidata":"https://www.wikidata.org/wiki/Q221179","display_name":"Streptococcus pneumoniae","level":3,"score":0.8985329866409302},{"id":"https://openalex.org/C10389963","wikidata":"https://www.wikidata.org/wiki/Q848328","display_name":"Serotype","level":2,"score":0.8106752634048462},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.7760700583457947},{"id":"https://openalex.org/C2778866548","wikidata":"https://www.wikidata.org/wiki/Q7205991","display_name":"Pneumococcal conjugate vaccine","level":4,"score":0.7507753372192383},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.7414027452468872},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6683278679847717},{"id":"https://openalex.org/C2776152631","wikidata":"https://www.wikidata.org/wiki/Q4366062","display_name":"Pneumococcal infections","level":4,"score":0.6384587287902832},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.5303776264190674},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5245882868766785},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.49035000801086426},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.46849948167800903},{"id":"https://openalex.org/C2778663903","wikidata":"https://www.wikidata.org/wiki/Q140018","display_name":"Streptococcaceae","level":3,"score":0.460233211517334},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3566630482673645},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.31106826663017273},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.22600427269935608},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11400964856147766},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.10619160532951355},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.06510278582572937},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.045434385538101196},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013296","descriptor_name":"Streptococcus pneumoniae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013296","descriptor_name":"Streptococcus pneumoniae","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D022242","descriptor_name":"Pneumococcal Vaccines","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D060885","descriptor_name":"Multiplex Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.1371/journal.pone.0050406","doi":"https://doi.org/10.1371/journal.pone.0050406","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0050406","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050406&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3514308","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3514308","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:23226519","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23226519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:ac83c48f-e314-4b9a-b3ac-d7e043f7c112","doi":null,"is_oa":true,"landing_page_url":"https://avesis.aybu.edu.tr/publication/details/ac83c48f-e314-4b9a-b3ac-d7e043f7c112/oai","pdf_url":"https://avesis.aybu.edu.tr/publication/details/ac83c48f-e314-4b9a-b3ac-d7e043f7c112/oai/document.pdf","source":{"id":"https://openalex.org/S7407055213","display_name":"AYBU AVESIS","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.800.8992","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.800.8992","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/58/a5/pone.0050406.PMC3514308.pdf","raw_type":"text"},{"id":"pmh:oai:acikerisim.kku.edu.tr:20.500.12587/5143","doi":null,"is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0050406","pdf_url":null,"source":{"id":"https://openalex.org/S7407055314","display_name":"K\u0131r\u0131kkale University Institutional Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:doaj.org/article:18004b8623de4a49a236202c36f4516c","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/18004b8623de4a49a236202c36f4516c","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 7, Iss 12, p e50406 (2012)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0050406","doi":"https://doi.org/10.1371/journal.pone.0050406","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0050406","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050406&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320853","display_name":"Public Health Agency","ror":"https://ror.org/03ek62e72"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1983207263.pdf","grobid_xml":"https://content.openalex.org/works/W1983207263.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W967332817","https://openalex.org/W1865523903","https://openalex.org/W1965713117","https://openalex.org/W1981007703","https://openalex.org/W2000499723","https://openalex.org/W2014427579","https://openalex.org/W2015638534","https://openalex.org/W2020192551","https://openalex.org/W2024007242","https://openalex.org/W2027707941","https://openalex.org/W2029274790","https://openalex.org/W2082596529","https://openalex.org/W2098218091","https://openalex.org/W2103637444","https://openalex.org/W2108130630","https://openalex.org/W2108869655","https://openalex.org/W2116281719","https://openalex.org/W2116813766","https://openalex.org/W2117383615","https://openalex.org/W2133679549","https://openalex.org/W2135965844","https://openalex.org/W2140844843","https://openalex.org/W2141828980","https://openalex.org/W2149176649","https://openalex.org/W2150343901","https://openalex.org/W2158289519","https://openalex.org/W2165495755","https://openalex.org/W2168952114","https://openalex.org/W2170022558","https://openalex.org/W2951085858","https://openalex.org/W4299364571","https://openalex.org/W4300336165"],"related_works":["https://openalex.org/W2538044119","https://openalex.org/W2069773013","https://openalex.org/W2753376950","https://openalex.org/W2133679549","https://openalex.org/W2020192551","https://openalex.org/W2997904996","https://openalex.org/W1972337775","https://openalex.org/W3004083133","https://openalex.org/W7099134293","https://openalex.org/W4221061134"],"abstract_inverted_index":{"The":[0,92],"life-threatening":[1],"illnesses":[2],"caused":[3],"by":[4,90,117],"Streptococcus":[5],"pneumoniae":[6,58],"have":[7],"been":[8,49],"declined":[9],"significantly":[10,146],"after":[11],"the":[12,21,28,36,99,131,148],"use":[13,149],"of":[14,20,31,46,56,67,94,113,127,150],"pneumococcal":[15,33,115],"conjugate":[16],"vaccines.":[17],"Continuous":[18],"monitoring":[19],"vaccine":[22],"serogroups/types":[23,55,88],"is":[24],"necessary":[25],"to":[26],"follow":[27],"changing":[29],"epidemiology":[30],"invasive":[32],"diseases.":[34],"Recently,":[35],"sequential":[37],"multiplex":[38,70,95,133],"PCR":[39,71,96,134],"approach,":[40],"which":[41],"uses":[42],"several":[43],"different":[44],"sets":[45],"reactions,":[47],"has":[48],"commonly":[50],"adopted":[51],"for":[52],"determining":[53],"capsular":[54],"S.":[57],"isolates.":[59],"In":[60,124],"our":[61,125],"study,":[62],"we":[63],"focused":[64],"on":[65],"development":[66],"a":[68,105,114,120],"one-step":[69,132],"assay":[72,135],"detecting":[73],"all":[74],"1,":[75],"3,":[76],"4,":[77],"5,":[78],"6A/B,":[79],"7F,":[80],"9V,":[81],"14,":[82],"18C,":[83],"19A,":[84],"19F":[85],"and":[86,98,110,139],"23F":[87],"targeted":[89],"PCV13.":[91],"content":[93],"mix":[97],"cycling":[100],"conditions":[101],"were":[102],"optimized":[103],"in":[104],"manner":[106],"that":[107,142],"allowed":[108],"rapid":[109],"accurate":[111],"serotyping":[112],"isolate":[116],"performing":[118],"only":[119],"single":[121],"amplification":[122],"reaction.":[123],"study":[126],"182":[128],"clinical":[129],"isolates,":[130],"exhibited":[136],"100%":[137],"sensitivity":[138],"specificity,":[140],"suggesting":[141],"its":[143],"utilization":[144],"can":[145],"reduce":[147],"traditional":[151],"antiserum":[152],"method":[153],"requiring":[154],"expensive":[155],"reagents.":[156]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}