{"id":"https://openalex.org/W1982967855","doi":"https://doi.org/10.1186/1756-0500-3-278","title":"High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program","display_name":"High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program","publication_year":2010,"publication_date":"2010-11-04","ids":{"openalex":"https://openalex.org/W1982967855","doi":"https://doi.org/10.1186/1756-0500-3-278","mag":"1982967855","pmid":"https://pubmed.ncbi.nlm.nih.gov/21047438","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2987874"},"language":"en","primary_location":{"id":"doi:10.1186/1756-0500-3-278","doi":"https://doi.org/10.1186/1756-0500-3-278","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1756-0500-3-278","pdf_url":"https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/1756-0500-3-278","source":{"id":"https://openalex.org/S100014455","display_name":"BMC Research Notes","issn_l":"1756-0500","issn":["1756-0500"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Research Notes","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/1756-0500-3-278","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001141561","display_name":"Norhan S. Sheraba","orcid":"https://orcid.org/0000-0001-8071-9514","observed_orcids":["https://orcid.org/0000-0001-8071-9514"]},"institutions":[{"id":"https://openalex.org/I145487455","display_name":"Cairo University","ror":"https://ror.org/03q21mh05","country_code":"EG","type":"education","lineage":["https://openalex.org/I145487455"]},{"id":"https://openalex.org/I4210134127","display_name":"Vacsera (Egypt)","ror":"https://ror.org/03nh6bz87","country_code":"EG","type":"company","lineage":["https://openalex.org/I4210134127"]}],"countries":["EG"],"is_corresponding":false,"raw_author_name":"Norhan S Sheraba","raw_affiliation_strings":["Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt. aymen.yassin@live.com","VACSERA, The Holding Company for Biological Products &, Vaccines, Egypt"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt. aymen.yassin@live.com","institution_ids":["https://openalex.org/I145487455"]},{"raw_affiliation_string":"VACSERA, The Holding Company for Biological Products &, Vaccines, Egypt","institution_ids":["https://openalex.org/I4210134127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087057508","display_name":"Aymen S. Yassin","orcid":"https://orcid.org/0000-0002-4423-164X","observed_orcids":["https://orcid.org/0000-0002-4423-164X"]},"institutions":[{"id":"https://openalex.org/I145487455","display_name":"Cairo University","ror":"https://ror.org/03q21mh05","country_code":"EG","type":"education","lineage":["https://openalex.org/I145487455"]}],"countries":["EG"],"is_corresponding":true,"raw_author_name":"Aymen S Yassin","raw_affiliation_strings":["Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt","Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt","institution_ids":["https://openalex.org/I145487455"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt;","institution_ids":["https://openalex.org/I145487455"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084120285","display_name":"Magdy Aly Amin","orcid":"https://orcid.org/0000-0002-9292-7115","observed_orcids":["https://orcid.org/0000-0002-9292-7115"]},"institutions":[{"id":"https://openalex.org/I145487455","display_name":"Cairo University","ror":"https://ror.org/03q21mh05","country_code":"EG","type":"education","lineage":["https://openalex.org/I145487455"]}],"countries":["EG"],"is_corresponding":false,"raw_author_name":"Magdy A Amin","raw_affiliation_strings":["Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt","Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt","institution_ids":["https://openalex.org/I145487455"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt;","institution_ids":["https://openalex.org/I145487455"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5087057508"],"corresponding_institution_ids":["https://openalex.org/I145487455"],"apc_list":{"value":1690,"currency":"USD","value_usd":1690},"apc_paid":{"value":1690,"currency":"USD","value_usd":1690},"fwci":0.5411,"has_fulltext":true,"cited_by_count":11,"citation_normalized_percentile":{"value":0.67738714,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"3","issue":"1","first_page":"278","last_page":"278"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9467999935150146,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9467999935150146,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.050999999046325684,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/staphylococcus","display_name":"Staphylococcus","score":0.9929999709129333},{"id":"https://openalex.org/keywords/molecular-identification","display_name":"molecular identification","score":0.6359999775886536},{"id":"https://openalex.org/keywords/environmental-monitoring","display_name":"environmental monitoring","score":0.9409999847412109},{"id":"https://openalex.org/keywords/glyceraldehyde-3-phosphate-dehydrogenase","display_name":"glyceraldehyde-3-phosphate dehydrogenase","score":0.8790000081062317},{"id":"https://openalex.org/keywords/pcr-fingerprinting","display_name":"PCR fingerprinting","score":0.8619999885559082}],"concepts":[{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.6614649295806885},{"id":"https://openalex.org/C2779288629","wikidata":"https://www.wikidata.org/wiki/Q199695","display_name":"Staphylococcus","level":4,"score":0.603196382522583},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5356743931770325},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4763603210449219},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.457963764667511},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.43175825476646423},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.42120885848999023},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.41918933391571045},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.25327688455581665},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.23857948184013367},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1498863399028778},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.06651607155799866}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1186/1756-0500-3-278","doi":"https://doi.org/10.1186/1756-0500-3-278","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1756-0500-3-278","pdf_url":"https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/1756-0500-3-278","source":{"id":"https://openalex.org/S100014455","display_name":"BMC Research Notes","issn_l":"1756-0500","issn":["1756-0500"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Research Notes","raw_type":"journal-article"},{"id":"pmid:21047438","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21047438","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC research notes","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2987874","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2987874/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Res Notes","raw_type":"Text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.292.9017","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.9017","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/21/10/BMC_Res_Notes_2010_Nov_4_3_278.tar.gz","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:f9d3a26d77b2411dbe43c33594a4e1e7","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/f9d3a26d77b2411dbe43c33594a4e1e7","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Research Notes, Vol 3, Iss 1, p 278 (2010)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/1756-0500-3-278","doi":"https://doi.org/10.1186/1756-0500-3-278","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1756-0500-3-278","pdf_url":"https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/1756-0500-3-278","source":{"id":"https://openalex.org/S100014455","display_name":"BMC Research Notes","issn_l":"1756-0500","issn":["1756-0500"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Research Notes","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1982967855.pdf","grobid_xml":"https://content.openalex.org/works/W1982967855.grobid-xml"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W1603607002","https://openalex.org/W1703754009","https://openalex.org/W1842375866","https://openalex.org/W1844896024","https://openalex.org/W1867281131","https://openalex.org/W1898083917","https://openalex.org/W1950405859","https://openalex.org/W1979932265","https://openalex.org/W1996862789","https://openalex.org/W1998847137","https://openalex.org/W2009594412","https://openalex.org/W2012165681","https://openalex.org/W2018528185","https://openalex.org/W2021841732","https://openalex.org/W2031189100","https://openalex.org/W2033554127","https://openalex.org/W2044039002","https://openalex.org/W2052338076","https://openalex.org/W2090706510","https://openalex.org/W2096045817","https://openalex.org/W2099660265","https://openalex.org/W2100797518","https://openalex.org/W2101579434","https://openalex.org/W2104325460","https://openalex.org/W2107673359","https://openalex.org/W2109459211","https://openalex.org/W2114688294","https://openalex.org/W2117646346","https://openalex.org/W2120693070","https://openalex.org/W2146442541","https://openalex.org/W2147423362","https://openalex.org/W2148614009","https://openalex.org/W2150990827","https://openalex.org/W2154292199","https://openalex.org/W2160175271"],"related_works":["https://openalex.org/W7097477966","https://openalex.org/W1575092789","https://openalex.org/W1973721789","https://openalex.org/W2794141338","https://openalex.org/W2097567009","https://openalex.org/W2418236397","https://openalex.org/W2114454434","https://openalex.org/W2097073628","https://openalex.org/W2706503466","https://openalex.org/W2143370576"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,61],"staphylococci":[2,63],"are":[3],"one":[4],"of":[5,49,70,83,122,127],"the":[6,37,47,58,68,120],"most":[7],"common":[8],"environmental":[9,30],"isolates":[10],"found":[11],"in":[12,28,67,104,119],"clean":[13],"room":[14],"facility.":[15],"Consequently,":[16],"isolation":[17],"followed":[18],"by":[19],"comprehensive":[20],"and":[21,52,117],"accurate":[22],"identification":[23,40,69,121],"is":[24],"an":[25],"essential":[26],"step":[27],"any":[29],"monitoring":[31],"program.":[32],"FINDINGS:":[33],"We":[34],"have":[35],"used":[36,103],"API":[38,62,130],"Staph":[39,131],"kit":[41],"(bioM\u00e9rieux,":[42],"France)":[43],"which":[44,107,132],"depends":[45,108,133],"on":[46,79,109,134],"expression":[48],"metabolic":[50],"activities":[51],"or":[53],"morphological":[54],"features":[55,111],"to":[56],"identify":[57],"Staphylococcus":[59,94,128],"isolates.":[60,95],"showed":[64,99],"low":[65],"sensitivity":[66],"some":[71],"species,":[72],"so":[73],"we":[74],"performed":[75],"molecular":[76],"methods":[77],"based":[78,81],"PCR":[80,101],"fingerprinting":[82],"glyceraldehyde-3-phosphate":[84],"dehydrogenase":[85],"encoding":[86],"gene":[87],"as":[88],"useful":[89],"taxonomic":[90],"tool":[91],"for":[92],"examining":[93],"CONCLUSIONS:":[96],"Our":[97],"results":[98],"that":[100],"protocol":[102],"this":[105],"study":[106],"genotypic":[110],"was":[112],"relatively":[113],"accurate,":[114],"rapid,":[115],"sensitive":[116],"superior":[118],"at":[123],"least":[124],"7":[125],"species":[126],"than":[129],"phenotypic":[135],"features.":[136]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}