{"id":"https://openalex.org/W1982923843","doi":"https://doi.org/10.1111/j.1574-6968.2002.tb11175.x","title":"Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene","display_name":"Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene","publication_year":2002,"publication_date":"2002-04-01","ids":{"openalex":"https://openalex.org/W1982923843","doi":"https://doi.org/10.1111/j.1574-6968.2002.tb11175.x","mag":"1982923843","pmid":"https://pubmed.ncbi.nlm.nih.gov/12023093"},"language":"en","primary_location":{"id":"doi:10.1111/j.1574-6968.2002.tb11175.x","doi":"https://doi.org/10.1111/j.1574-6968.2002.tb11175.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1574-6968.2002.tb11175.x","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W1982923843"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029865230","display_name":"Chikara Kaito","orcid":"https://orcid.org/0000-0002-2895-3386","observed_orcids":["https://orcid.org/0000-0002-2895-3386"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Chikara Kaito","raw_affiliation_strings":["Graduate School of Pharmaceutical Sciences, University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, 113-0033, Japan","Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20101, 7\u2010Chome, Hongo, Bunkyo\u2010ku, Tokyo 113\u20100033, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Pharmaceutical Sciences, University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20101, 7\u2010Chome, Hongo, Bunkyo\u2010ku, Tokyo 113\u20100033, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kenji Kurokawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenji Kurokawa","raw_affiliation_strings":["Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047095568","display_name":"Muktadir S. Hossain","orcid":"https://orcid.org/0000-0002-7239-4577","observed_orcids":["https://orcid.org/0000-0002-7239-4577"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Muktadir Shahid Hossain","raw_affiliation_strings":["Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016202171","display_name":"Nobuyoshi Akimitsu","orcid":"https://orcid.org/0000-0002-3190-2942","observed_orcids":["https://orcid.org/0000-0002-3190-2942"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nobuyoshi Akimitsu","raw_affiliation_strings":["Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003851517","display_name":"Kazuhisa Sekimizu","orcid":"https://orcid.org/0000-0002-2849-8432","observed_orcids":["https://orcid.org/0000-0002-2849-8432"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Kazuhisa Sekimizu","raw_affiliation_strings":["Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Pharmaceutical Sciences, University of Tokyo, 3\u20131, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5003851517"],"corresponding_institution_ids":["https://openalex.org/I74801974"],"apc_list":{"value":2500,"currency":"GBP","value_usd":3297},"apc_paid":null,"fwci":0.591,"has_fulltext":true,"cited_by_count":25,"citation_normalized_percentile":{"value":0.6437353,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"210","issue":"1","first_page":"157","last_page":"164"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.95169997215271,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.95169997215271,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.029100000858306885,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.01759999990463257,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.9919999837875366},{"id":"https://openalex.org/keywords/temperature-sensitive-mutants","display_name":"temperature-sensitive mutants","score":0.996999979019165},{"id":"https://openalex.org/keywords/dna-replication","display_name":"DNA replication","score":0.6919999718666077},{"id":"https://openalex.org/keywords/uv-sensitivity","display_name":"UV sensitivity","score":0.7269999980926514},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.9940000176429749},{"id":"https://openalex.org/keywords/antibiotic-targets","display_name":"antibiotic targets","score":0.7440000176429749},{"id":"https://openalex.org/keywords/genetic-complementation","display_name":"gene complementation","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C116107829","wikidata":"https://www.wikidata.org/wiki/Q24724024","display_name":"dnaB helicase","level":5,"score":0.9281471967697144},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6364702582359314},{"id":"https://openalex.org/C107827726","wikidata":"https://www.wikidata.org/wiki/Q673206","display_name":"DnaA","level":5,"score":0.5900059342384338},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5049751400947571},{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.49849867820739746},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46453434228897095},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.41817963123321533},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.40836894512176514},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3954848051071167},{"id":"https://openalex.org/C161223559","wikidata":"https://www.wikidata.org/wiki/Q138864","display_name":"Helicase","level":4,"score":0.3058706521987915},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.23003703355789185},{"id":"https://openalex.org/C104690221","wikidata":"https://www.wikidata.org/wiki/Q5165872","display_name":"Control of chromosome duplication","level":4,"score":0.1662088930606842},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.07551851868629456},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004265","descriptor_name":"DNA Helicases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004265","descriptor_name":"DNA Helicases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000528","qualifier_name":"radiation effects","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014466","descriptor_name":"Ultraviolet Rays","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D053488","descriptor_name":"DnaB Helicases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1111/j.1574-6968.2002.tb11175.x","doi":"https://doi.org/10.1111/j.1574-6968.2002.tb11175.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1574-6968.2002.tb11175.x","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},{"id":"doi:10.1016/s0378-1097(02)00603-1","doi":"https://doi.org/10.1016/s0378-1097(02)00603-1","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0378-1097(02)00603-1","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},{"id":"pmid:12023093","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12023093","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS microbiology letters","raw_type":"Journal Article"},{"id":"pmh:oai:irdb.nii.ac.jp:00953:0006728657","doi":null,"is_oa":false,"landing_page_url":"https://teikyo-u.repo.nii.ac.jp/records/2013266","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W150420254","https://openalex.org/W1605117055","https://openalex.org/W1651468080","https://openalex.org/W1801750535","https://openalex.org/W1867282236","https://openalex.org/W1991061781","https://openalex.org/W2018405580","https://openalex.org/W2030193787","https://openalex.org/W2032209646","https://openalex.org/W2040226264","https://openalex.org/W2045979643","https://openalex.org/W2073624860","https://openalex.org/W2089871249","https://openalex.org/W2119395436","https://openalex.org/W2121942815","https://openalex.org/W2139316119","https://openalex.org/W2141291479","https://openalex.org/W2148734696","https://openalex.org/W2163827697","https://openalex.org/W2170367123","https://openalex.org/W2401494917"],"related_works":["https://openalex.org/W2401494917","https://openalex.org/W2013814104","https://openalex.org/W2066340610","https://openalex.org/W2141291479","https://openalex.org/W2098169274","https://openalex.org/W2037418667","https://openalex.org/W1651468080","https://openalex.org/W1981266359","https://openalex.org/W254593455","https://openalex.org/W1979561439"],"abstract_inverted_index":{"A":[0],"protein":[1],"encoded":[2],"by":[3,38],"the":[4,39,53,59,65,78],"Staphylococcus":[5],"aureus":[6,55,100],"dnaC":[7,40,56,79],"gene":[8,57,80],"has":[9,47],"44%":[10],"and":[11,18],"58%":[12],"homology":[13],"with":[14],"Escherichia":[15],"coli":[16],"DnaB":[17],"Bacillus":[19],"subtilis":[20],"DnaC":[21,101],"replicative":[22,60],"DNA":[23,42,61,84],"helicases,":[24],"respectively.":[25],"We":[26],"identified":[27],"five":[28],"mutant":[29,90],"strains":[30],"whose":[31],"temperature-sensitive":[32],"colony":[33],"formation":[34],"phenotypes":[35],"were":[36,70],"complemented":[37],"gene.":[41],"replication":[43],"in":[44,83],"these":[45],"mutants":[46,69],"a":[48,94,104],"fast-stop":[49],"phenotype,":[50],"indicating":[51],"that":[52,77,98],"S.":[54,99],"encodes":[58],"helicase":[62,102],"required":[63],"for":[64,107],"elongation":[66],"step.":[67],"These":[68],"also":[71],"sensitive":[72],"to":[73],"UV":[74],"irradiation,":[75],"suggesting":[76,97],"is":[81,103],"involved":[82],"repair.":[85],"The":[86],"number":[87],"of":[88],"viable":[89],"cells":[91],"decreased":[92],"at":[93],"non-permissive":[95],"temperature,":[96],"promising":[105],"target":[106],"antibiotics":[108],"providing":[109],"bactericidal":[110],"effects.":[111]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}