{"id":"https://openalex.org/W1982255673","doi":"https://doi.org/10.1002/pmic.200700604","title":"Subcellular fractionation of TGF\u2010\u03b21\u2010stimulated lung epithelial cells: A novel proteomic approach for identifying signaling intermediates","display_name":"Subcellular fractionation of TGF\u2010\u03b21\u2010stimulated lung epithelial cells: A novel proteomic approach for identifying signaling intermediates","publication_year":2009,"publication_date":"2009-02-27","ids":{"openalex":"https://openalex.org/W1982255673","doi":"https://doi.org/10.1002/pmic.200700604","mag":"1982255673","pmid":"https://pubmed.ncbi.nlm.nih.gov/19253281"},"language":"en","primary_location":{"id":"doi:10.1002/pmic.200700604","doi":"https://doi.org/10.1002/pmic.200700604","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pmic.200700604","pdf_url":null,"source":{"id":"https://openalex.org/S182655450","display_name":"PROTEOMICS","issn_l":"1615-9853","issn":["1615-9853","1615-9861"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PROTEOMICS","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001723130","display_name":"Jadranka Milo\u0161evi\u0107","orcid":"https://orcid.org/0000-0003-0002-8727","observed_orcids":["https://orcid.org/0000-0003-0002-8727"]},"institutions":[{"id":"https://openalex.org/I200763008","display_name":"Justus-Liebig-Universit\u00e4t Gie\u00dfen","ror":"https://ror.org/033eqas34","country_code":"DE","type":"education","lineage":["https://openalex.org/I200763008"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Jadranka Milosevic","raw_affiliation_strings":["Department of Medicine, University of Giessen Lung Center, Giessen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Giessen Lung Center, Giessen, Germany","institution_ids":["https://openalex.org/I200763008"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005323074","display_name":"Patrick Bulau","orcid":"https://orcid.org/0000-0002-7121-2954","observed_orcids":["https://orcid.org/0000-0002-7121-2954"]},"institutions":[{"id":"https://openalex.org/I200763008","display_name":"Justus-Liebig-Universit\u00e4t Gie\u00dfen","ror":"https://ror.org/033eqas34","country_code":"DE","type":"education","lineage":["https://openalex.org/I200763008"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Patrick Bulau","raw_affiliation_strings":["Department of Medicine, University of Giessen Lung Center, Giessen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Giessen Lung Center, Giessen, Germany","institution_ids":["https://openalex.org/I200763008"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014404761","display_name":"Ejvind M\u00f8rtz","orcid":"https://orcid.org/0000-0002-4381-1546","observed_orcids":["https://orcid.org/0000-0002-4381-1546"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ejvind Mortz","raw_affiliation_strings":["Alphalyse A/S, Odense, Denmark, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Alphalyse A/S, Odense, Denmark, Germany","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009672774","display_name":"Oliver Eickelberg","orcid":"https://orcid.org/0000-0001-7170-0360","observed_orcids":["https://orcid.org/0000-0001-7170-0360"]},"institutions":[{"id":"https://openalex.org/I3018134672","display_name":"Helmholtz Munich","ror":"https://ror.org/00cfam450","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I3018134672"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Oliver Eickelberg","raw_affiliation_strings":["Institute of Lung Biology and Disease (ILBD), Helmholtz Zentrum M\u00fcnchen, Neuherberg/M\u00fcnchen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Lung Biology and Disease (ILBD), Helmholtz Zentrum M\u00fcnchen, Neuherberg/M\u00fcnchen, Germany","institution_ids":["https://openalex.org/I3018134672"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4650,"currency":"USD","value_usd":4650},"apc_paid":null,"fwci":1.0725,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.73660245,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"9","issue":"5","first_page":"1230","last_page":"1240"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11501","display_name":"TGF-\u03b2 signaling in diseases","score":0.9726999998092651,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11501","display_name":"TGF-\u03b2 signaling in diseases","score":0.9726999998092651,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.016599999740719795,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tgf-beta1-signaling","display_name":"TGF-\u03b21 signaling","score":0.984000027179718},{"id":"https://openalex.org/keywords/lung-epithelial-cells","display_name":"lung epithelial cells","score":0.9539999961853027},{"id":"https://openalex.org/keywords/a549-cells","display_name":"A549 cells","score":0.9020000100135803},{"id":"https://openalex.org/keywords/subcellular-fractionation","display_name":"subcellular fractionation","score":0.9750000238418579},{"id":"https://openalex.org/keywords/smad-signaling","display_name":"Smad signaling","score":0.47600001096725464},{"id":"https://openalex.org/keywords/nucleocytoplasmic-shuttling","display_name":"nucleocytoplasmic shuttling","score":0.7850000262260437},{"id":"https://openalex.org/keywords/khsrp","display_name":"KHSRP","score":0.777999997138977},{"id":"https://openalex.org/keywords/fubp1","display_name":"FUBP1","score":0.9919999837875366},{"id":"https://openalex.org/keywords/hnrnpl","display_name":"hnRNP L","score":0.9869999885559082},{"id":"https://openalex.org/keywords/rna-binding-proteins","display_name":"RNA-binding proteins","score":0.7829999923706055},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.6039999723434448}],"concepts":[{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6396729946136475},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.6169770359992981},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6100077629089355},{"id":"https://openalex.org/C2781080636","wikidata":"https://www.wikidata.org/wiki/Q700000","display_name":"SMAD","level":3,"score":0.4982621669769287},{"id":"https://openalex.org/C2778229498","wikidata":"https://www.wikidata.org/wiki/Q24785672","display_name":"Transforming growth factor beta","level":3,"score":0.47958341240882874},{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.41692879796028137},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.2413601577281952}],"mesh":[{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008168","descriptor_name":"Lung","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D008168","descriptor_name":"Lung","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015180","descriptor_name":"Electrophoresis, Gel, Two-Dimensional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D053773","descriptor_name":"Transforming Growth Factor beta1","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/pmic.200700604","doi":"https://doi.org/10.1002/pmic.200700604","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pmic.200700604","pdf_url":null,"source":{"id":"https://openalex.org/S182655450","display_name":"PROTEOMICS","issn_l":"1615-9853","issn":["1615-9853","1615-9861"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PROTEOMICS","raw_type":"journal-article"},{"id":"pmid:19253281","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19253281","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteomics","raw_type":"Journal Article"},{"id":"pmh:oai:opus-zb.helmholtz-muenchen.de:1497","doi":null,"is_oa":false,"landing_page_url":"https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=1497","pdf_url":null,"source":{"id":"https://openalex.org/S4377196115","display_name":"Site cant be reached","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Proteomics 9, 1230-1240 (2009)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":51,"referenced_works":["https://openalex.org/W1636022999","https://openalex.org/W1664757920","https://openalex.org/W1938683170","https://openalex.org/W1940920025","https://openalex.org/W1964245716","https://openalex.org/W1969741969","https://openalex.org/W1973862631","https://openalex.org/W1977950302","https://openalex.org/W1978146894","https://openalex.org/W1990232129","https://openalex.org/W1995978677","https://openalex.org/W1996279504","https://openalex.org/W1997105701","https://openalex.org/W2005587846","https://openalex.org/W2023209792","https://openalex.org/W2026273737","https://openalex.org/W2027516367","https://openalex.org/W2031066980","https://openalex.org/W2033398177","https://openalex.org/W2035653659","https://openalex.org/W2036109241","https://openalex.org/W2040310860","https://openalex.org/W2041518157","https://openalex.org/W2051083202","https://openalex.org/W2052668460","https://openalex.org/W2060603808","https://openalex.org/W2063468500","https://openalex.org/W2075341017","https://openalex.org/W2077643233","https://openalex.org/W2078594804","https://openalex.org/W2083340704","https://openalex.org/W2087672655","https://openalex.org/W2089171495","https://openalex.org/W2089813874","https://openalex.org/W2092641791","https://openalex.org/W2103123117","https://openalex.org/W2104570676","https://openalex.org/W2115078186","https://openalex.org/W2115478133","https://openalex.org/W2136819827","https://openalex.org/W2139043430","https://openalex.org/W2141261198","https://openalex.org/W2142707412","https://openalex.org/W2149717321","https://openalex.org/W2153787061","https://openalex.org/W2154568166","https://openalex.org/W2166644606","https://openalex.org/W2167974691","https://openalex.org/W2169062791","https://openalex.org/W3023110197","https://openalex.org/W4234452205"],"related_works":["https://openalex.org/W6907526098","https://openalex.org/W2142722387","https://openalex.org/W2142707412","https://openalex.org/W2040270518","https://openalex.org/W1991790628","https://openalex.org/W2082465157","https://openalex.org/W2082144273","https://openalex.org/W3106779501","https://openalex.org/W2148569120","https://openalex.org/W2741476690"],"abstract_inverted_index":{"Members":[0],"of":[1,11,29,38,46,68,131,177],"the":[2,53,128,175],"transforming":[3],"growth":[4],"factor":[5],"(TGF)-beta":[6],"superfamily":[7],"are":[8],"key":[9],"regulators":[10],"lung":[12,100,108],"development":[13],"and":[14,34,43,120,143,163],"homeostasis,":[15],"in":[16,93,99,127,149,153],"particular":[17],"by":[18,58,65,161],"controlling":[19],"alveolar/bronchial":[20],"epithelial":[21,101,109],"cell":[22],"function.":[23],"TGF-beta":[24,59,94],"signaling":[25,55,73,95,180],"involves":[26],"ligand-dependent":[27],"activation":[28,33],"receptor":[30],"serine/threonine":[31],"kinases,":[32],"subsequent":[35],"nuclear":[36,151],"translocation":[37],"pathway-specific":[39],"transcription":[40],"factors":[41],"(Smads),":[42],"ultimately,":[44],"modulation":[45],"gene":[47],"expression.":[48],"While":[49],"Smad-dependent":[50],"responses":[51],"represent":[52,169],"primary":[54],"components":[56],"activated":[57],"receptors,":[60],"their":[61,150],"function":[62],"is":[63],"controlled":[64],"a":[66,124,147,170],"variety":[67],"cofactors.":[69],"In":[70,166],"addition,":[71],"alternative":[72],"systems":[74],"mediating":[75],"TGF-beta-induced":[76],"effects":[77],"have":[78,104],"recently":[79],"been":[80],"described":[81],"such":[82],"as":[83],"MAP":[84],"kinase":[85],"pathways.":[86],"To":[87],"uncover":[88],"novel":[89,172],"proteins":[90],"that":[91],"participate":[92],"via":[96],"nuclear/cytoplasmic":[97],"shuttling":[98],"cells,":[102,110],"we":[103,168],"analyzed":[105],"A549":[106],"human":[107],"using":[111],"subcellular":[112],"fractionation":[113],"combined":[114],"with":[115,146],"2-D":[116],"PAGE,":[117],"tryptic":[118],"digestion,":[119],"MS.":[121],"We":[122],"identified":[123],"rapid":[125],"increase":[126],"cytosolic":[129],"localization":[130,152],"KH-type":[132],"splicing":[133],"regulatory":[134],"protein":[135,140],"(KHSRP),":[136],"far":[137],"upstream":[138],"element-binding":[139],"(FUBP1),":[141],"hnRNP-L,":[142],"hnRNP-H1,":[144],"concomitant":[145],"decrease":[148],"response":[154],"to":[155],"TGF-beta1.":[156],"Proteomic":[157],"data":[158],"were":[159],"confirmed":[160],"immunofluorescence":[162],"immunoblot":[164],"analyses.":[165],"summary,":[167],"powerful":[171],"technology":[173],"for":[174],"identification":[176],"previously":[178],"unknown":[179],"intermediates.":[181]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}