{"id":"https://openalex.org/W198143347","doi":null,"title":"Generation and characterization of antibodies for proteomics research","display_name":"Generation and characterization of antibodies for proteomics research","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W198143347","mag":"198143347"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:kth-11425","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11425","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11425","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Karin Larsson","orcid":"https://orcid.org/0000-0002-6403-2736","observed_orcids":["https://orcid.org/0000-0002-6403-2736"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Karin Larsson","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-6403-2736","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8701000213623047,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8701000213623047,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.0908999964594841,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.03139999881386757,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/monoclonal-antibody-production","display_name":"monoclonal antibody production","score":0.781000018119812},{"id":"https://openalex.org/keywords/prest","display_name":"prests","score":0.6489999890327454},{"id":"https://openalex.org/keywords/antibody-purification","display_name":"antibody purification","score":0.7950000166893005},{"id":"https://openalex.org/keywords/protein-microarrays","display_name":"protein microarrays","score":0.7360000014305115},{"id":"https://openalex.org/keywords/human-protein-atlas","display_name":"Human Protein Atlas","score":0.8980000019073486},{"id":"https://openalex.org/keywords/antigen-selection","display_name":"antigen selection","score":0.5580000281333923},{"id":"https://openalex.org/keywords/epitope-mapping","display_name":"epitope mapping","score":0.7699999809265137},{"id":"https://openalex.org/keywords/cytokeratin-17","display_name":"cytokeratin 17","score":0.8460000157356262},{"id":"https://openalex.org/keywords/intermediate-filaments","display_name":"intermediate filaments","score":0.8539999723434448},{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.8479999899864197},{"id":"https://openalex.org/keywords/receptor-internalization","display_name":"receptor internalization","score":0.3659999966621399},{"id":"https://openalex.org/keywords/antibody-specificity","display_name":"antibody specificity","score":0.375},{"id":"https://openalex.org/keywords/cross-reactivity","display_name":"cross-reactivity","score":0.6589999794960022},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.45899999141693115}],"concepts":[{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.4450039863586426},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.43930357694625854},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.38760703802108765},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.380352258682251},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.26571857929229736},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22088375687599182},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.13900399208068848},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.10305926203727722},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.07656136155128479},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:kth-11425","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11425","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:198143347","doi":null,"is_oa":false,"landing_page_url":"http://kth.diva-portal.org/smash/record.jsf?pid=diva2:275849","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:kth-11425","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11425","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":137,"referenced_works":["https://openalex.org/W47869444","https://openalex.org/W147473274","https://openalex.org/W1480851099","https://openalex.org/W1490628419","https://openalex.org/W1531451570","https://openalex.org/W1550351158","https://openalex.org/W1575542444","https://openalex.org/W1591942886","https://openalex.org/W1604588480","https://openalex.org/W1606423922","https://openalex.org/W1623903296","https://openalex.org/W1821392799","https://openalex.org/W1857616382","https://openalex.org/W1963903021","https://openalex.org/W1964396121","https://openalex.org/W1965544846","https://openalex.org/W1965706807","https://openalex.org/W1966326669","https://openalex.org/W1967040251","https://openalex.org/W1971142781","https://openalex.org/W1974596630","https://openalex.org/W1974830441","https://openalex.org/W1976801077","https://openalex.org/W1979449581","https://openalex.org/W1980104332","https://openalex.org/W1982622130","https://openalex.org/W1983494459","https://openalex.org/W1984492870","https://openalex.org/W1984606649","https://openalex.org/W1989053318","https://openalex.org/W1990557050","https://openalex.org/W1992629832","https://openalex.org/W1993575340","https://openalex.org/W1994040531","https://openalex.org/W1994664175","https://openalex.org/W1994802428","https://openalex.org/W1995089537","https://openalex.org/W1996298591","https://openalex.org/W1996820981","https://openalex.org/W1997113958","https://openalex.org/W1997458078","https://openalex.org/W1999700891","https://openalex.org/W2001184763","https://openalex.org/W2001394491","https://openalex.org/W2003470884","https://openalex.org/W2003593782","https://openalex.org/W2004841938","https://openalex.org/W2010039421","https://openalex.org/W2011261569","https://openalex.org/W2013091822","https://openalex.org/W2013620812","https://openalex.org/W2014099862","https://openalex.org/W2016456704","https://openalex.org/W2016693266","https://openalex.org/W2017217102","https://openalex.org/W2017661443","https://openalex.org/W2020155878","https://openalex.org/W2021144157","https://openalex.org/W2024298562","https://openalex.org/W2030522563","https://openalex.org/W2033664155","https://openalex.org/W2035758624","https://openalex.org/W2042655455","https://openalex.org/W2044503822","https://openalex.org/W2045340003","https://openalex.org/W2048333869","https://openalex.org/W2050105922","https://openalex.org/W2050466522","https://openalex.org/W2050826226","https://openalex.org/W2051448244","https://openalex.org/W2053418558","https://openalex.org/W2053559379","https://openalex.org/W2055547342","https://openalex.org/W2057520802","https://openalex.org/W2057782477","https://openalex.org/W2065625685","https://openalex.org/W2069848278","https://openalex.org/W2070360032","https://openalex.org/W2072959841","https://openalex.org/W2075097399","https://openalex.org/W2078896733","https://openalex.org/W2082122713","https://openalex.org/W2084855813","https://openalex.org/W2084938664","https://openalex.org/W2085168389","https://openalex.org/W2086195230","https://openalex.org/W2086889737","https://openalex.org/W2087810003","https://openalex.org/W2088324202","https://openalex.org/W2089282467","https://openalex.org/W2092803887","https://openalex.org/W2093828656","https://openalex.org/W2103719918","https://openalex.org/W2103735815","https://openalex.org/W2105128791","https://openalex.org/W2106150646","https://openalex.org/W2106845528","https://openalex.org/W2106876831","https://openalex.org/W2108796583","https://openalex.org/W2111817213","https://openalex.org/W2113344523","https://openalex.org/W2114630876","https://openalex.org/W2115008851","https://openalex.org/W2118577784","https://openalex.org/W2121418514","https://openalex.org/W2127230663","https://openalex.org/W2130314959","https://openalex.org/W2130684944","https://openalex.org/W2135565251","https://openalex.org/W2138546851","https://openalex.org/W2138664009","https://openalex.org/W2140471823","https://openalex.org/W2145799655","https://openalex.org/W2149464124","https://openalex.org/W2152996957","https://openalex.org/W2154404874","https://openalex.org/W2156770078","https://openalex.org/W2159847871","https://openalex.org/W2160813629","https://openalex.org/W2161574105","https://openalex.org/W2161859073","https://openalex.org/W2168909179","https://openalex.org/W2170937824","https://openalex.org/W2171486644","https://openalex.org/W2171938017","https://openalex.org/W2172028877","https://openalex.org/W2172029739","https://openalex.org/W2172116519","https://openalex.org/W2187813473","https://openalex.org/W2258179564","https://openalex.org/W2409893983","https://openalex.org/W2410583950","https://openalex.org/W2463829916","https://openalex.org/W2475348408","https://openalex.org/W2602020506","https://openalex.org/W2774846296","https://openalex.org/W2912078763"],"related_works":["https://openalex.org/W176618442","https://openalex.org/W1964396121","https://openalex.org/W2019496029","https://openalex.org/W2781060913","https://openalex.org/W82660935","https://openalex.org/W2128450902","https://openalex.org/W98516055","https://openalex.org/W2980404798","https://openalex.org/W154379509","https://openalex.org/W45615294"],"abstract_inverted_index":{"Specific":[0],"antibodies":[1,13,82,132,233,254,308,375,400,429,440],"are":[2],"invaluable":[3],"tools":[4],"for":[5,64,136,173,216,273,361,371,444],"proteomics":[6],"research.":[7],"The":[8,34,380,417],"availability":[9],"of":[10,20,30,36,53,75,124,170,182,201,223,253,277,292,307,336,352,385,387,410,449],"thoroughly":[11],"validated":[12],"will":[14],"help":[15],"to":[16,43,47,57,84,113,166,207,229,251,266,288,331,393,432,434],"improve":[17,179],"our":[18],"understanding":[19],"protein":[21,95,126,138,219],"expression,":[22],"localization":[23],"and":[24,28,51,56,68,103,117,178,205,214,239,302,305,383,397,406,423,452],"function;":[25],"fundamental":[26],"processes":[27],"features":[29],"all":[31,235],"living":[32],"organisms.":[33],"objectives":[35],"the":[37,49,73,76,122,160,168,180,224,236,274,293,303,332,337,342,350,358,398,414,428,439,445],"studies":[38],"in":[39,90,119,130,140,146,191,318,373,446],"this":[40,311],"thesis":[41],"were":[42,134,211,313,391,401,430,441],"develop":[44],"high-throughput":[45,92,115],"methods":[46],"facilitate":[48],"generation":[50,276],"purification":[52,109],"monospecific":[54,131],"antibodies,":[55],"address":[58],"problems":[59],"associated":[60],"with":[61,121,199,227,262,320],"antigen":[62,366],"selection":[63],"difficult":[65],"target":[66,312],"proteins":[67,86,268],"subsequent":[69],"validation":[70],"issues.":[71],"In":[72,164,279,437],"first":[74],"studies,":[77],"it":[78],"was":[79,111,189,297,369],"demonstrated":[80],"that":[81,133,150,427],"specific":[83],"human":[85,142,248,450],"could":[87],"be":[88,157],"generated":[89,309],"a":[91,114,125,151,175,185,192,270,300],"manner":[93],"using":[94,218,315,353],"epitope":[96,316,327],"signature":[97],"tags":[98],"(PrESTs)":[99],"as":[100,299,329,357],"both":[101,420],"antigens":[102,396],"affinity":[104],"ligands.":[105],"A":[106],"previously":[107],"developed":[108,190],"process":[110],"adapted":[112],"format":[116],"this,":[118],"combination":[120,319],"development":[123],"microarray":[127],"assay,":[128],"resulted":[129],"used":[135,298,442],"profiling":[137],"expression":[139],"48":[141],"tissues.":[143],"Data":[144],"obtained":[145,255],"these":[147],"analyses":[148,409,425],"suggest":[149],"complete":[152],"Human":[153],"Protein":[154],"Atlas":[155],"should":[156],"attainable":[158],"within":[159],"next":[161],"ten":[162,208],"years.":[163],"order":[165],"reduce":[167],"number":[169],"animals":[171,225],"needed":[172],"such":[174],"massive":[176],"project,":[177],"cost-efficiency":[181],"antibody":[183],"generation,":[184],"multiplex":[186],"immunization":[187],"strategy":[188,351,368],"further":[193,348],"study.":[194],"Antisera":[195],"from":[196,256,419],"rabbits":[197],"immunized":[198,226],"mixtures":[200],"two,":[202],"three,":[203],"five":[204],"up":[206,228],"different":[209],"PrESTs":[210,231,237,339],"successfully":[212,443],"purified":[213],"analyzed":[215,402],"specificity":[217,304],"arrays.":[220],"Almost":[221],"80%":[222],"three":[230,388],"yielded":[232,241],"towards":[234,376],"administered,":[238],"they":[240],"comparable":[242],"immunohistochemical":[243],"staining":[244,345],"patterns":[245],"(of":[246],"consecutive":[247],"tissue":[249],"sections)":[250],"those":[252],"traditional":[257],"single":[258,395],"PrEST":[259,362],"immunizations.":[260],"Proteins":[261],"highly":[263],"similar":[264],"sequences":[265],"other":[267],"present":[269],"major":[271],"challenge":[272],"proteome-wide":[275],"antibodies.":[278],"another":[280],"study,":[281],"Cytokeratin-17":[282,338],"which":[283],"displays":[284],"high":[285],"sequence":[286,354],"similarity":[287],"closely":[289],"related":[290],"members":[291],"intermediate":[294],"filament":[295],"family,":[296],"model":[301],"cross-reactivity":[306],"against":[310],"investigated":[314,370],"mapping":[317,328],"comparative":[321],"IHC":[322],"analyses.":[323],"Antibodies":[324],"identified":[325],"by":[326,403],"binding":[330],"most":[333,343],"unique":[334],"parts":[335],"also":[340],"showed":[341,426],"Cytokeratin-17-like":[344],"pattern,":[346],"thus":[347],"supporting":[349],"identity":[355],"scores":[356],"main":[359],"criteria":[360],"design.":[363],"An":[364],"alternative":[365],"design":[367],"use":[372],"raising":[374],"G-proteincoupled":[377],"receptors":[378],"(GPCRs).":[379],"extracellular":[381],"loops":[382],"N-terminus":[384],"each":[386],"selected":[389],"GPCRs":[390],"assembled":[392],"form":[394],"resulting":[399],"flow":[404,421],"cytometric":[405,422],"confocal":[407],"microscopic":[408],"cell":[411],"lines":[412],"over-expressing":[413],"respective":[415],"receptors.":[416],"results":[418],"immunofluorescence":[424],"able":[431],"bind":[433],"their":[435],"targets.":[436],"addition,":[438],"situ":[447],"analysis":[448],"brain":[451],"pancreatic":[453],"islet":[454],"cells.":[455]},"counts_by_year":[{"year":2017,"cited_by_count":2}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2016-06-24T00:00:00"}
