{"id":"https://openalex.org/W1981057166","doi":"https://doi.org/10.1021/bi952000v","title":"Poly(ethylene glycol)\u2212Lipid Conjugates Regulate the Calcium-Induced Fusion of Liposomes Composed of Phosphatidylethanolamine and Phosphatidylserine","display_name":"Poly(ethylene glycol)\u2212Lipid Conjugates Regulate the Calcium-Induced Fusion of Liposomes Composed of Phosphatidylethanolamine and Phosphatidylserine","publication_year":1996,"publication_date":"1996-01-01","ids":{"openalex":"https://openalex.org/W1981057166","doi":"https://doi.org/10.1021/bi952000v","mag":"1981057166","pmid":"https://pubmed.ncbi.nlm.nih.gov/8611565"},"language":"en","primary_location":{"id":"doi:10.1021/bi952000v","doi":"https://doi.org/10.1021/bi952000v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi952000v","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000409487","display_name":"John W. Holland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"John W. Holland","raw_affiliation_strings":["Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089285525","display_name":"Cathy Hui","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Cathy Hui","raw_affiliation_strings":["Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050350074","display_name":"Pieter R. Cullis","orcid":"https://orcid.org/0000-0001-9586-2508","observed_orcids":["https://orcid.org/0000-0001-9586-2508"]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Pieter R. Cullis","raw_affiliation_strings":["Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113900388","display_name":"Thomas D. Madden","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Thomas D. Madden","raw_affiliation_strings":["Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Therapeutics and Department of Biochemistry, University of British Columbia, Vancouver, BC, V6T lZ3, Canada","institution_ids":["https://openalex.org/I141945490"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I141945490"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":4.544,"has_fulltext":false,"cited_by_count":223,"citation_normalized_percentile":{"value":0.95632911,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":94,"max":100},"biblio":{"volume":"35","issue":"8","first_page":"2618","last_page":"2624"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.9380000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.9380000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.057500001043081284,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peg-lipid-conjugates","display_name":"PEG-lipid conjugates","score":0.9599999785423279},{"id":"https://openalex.org/keywords/liposome-fusion","display_name":"liposome fusion","score":0.796999990940094},{"id":"https://openalex.org/keywords/calcium-induced-fusion","display_name":"calcium-induced fusion","score":0.9610000252723694},{"id":"https://openalex.org/keywords/phosphatidylethanolamine","display_name":"phosphatidylethanolamine","score":0.9869999885559082},{"id":"https://openalex.org/keywords/phosphatidylserine","display_name":"phosphatidylserine","score":1.0},{"id":"https://openalex.org/keywords/steric-barrier","display_name":"steric barrier","score":0.8820000290870667},{"id":"https://openalex.org/keywords/peg-transfer-task","display_name":"peg transfer task","score":0.578000009059906},{"id":"https://openalex.org/keywords/fusogenic-liposomes","display_name":"fusogenic liposomes","score":0.75},{"id":"https://openalex.org/keywords/liposomal-drug-delivery","display_name":"liposomal drug delivery","score":0.7770000100135803},{"id":"https://openalex.org/keywords/lipid-saturation","display_name":"lipid saturation","score":0.492000013589859},{"id":"https://openalex.org/keywords/large-unilamellar-vesicles","display_name":"large unilamellar vesicles","score":0.7979999780654907}],"concepts":[{"id":"https://openalex.org/C185154212","wikidata":"https://www.wikidata.org/wiki/Q410454","display_name":"Liposome","level":2,"score":0.8506389856338501},{"id":"https://openalex.org/C2781170229","wikidata":"https://www.wikidata.org/wiki/Q426095","display_name":"Phosphatidylethanolamine","level":5,"score":0.7899243831634521},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7777228355407715},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.6625276207923889},{"id":"https://openalex.org/C2779637612","wikidata":"https://www.wikidata.org/wiki/Q2354337","display_name":"Phosphatidylserine","level":4,"score":0.6598526239395142},{"id":"https://openalex.org/C2777516009","wikidata":"https://www.wikidata.org/wiki/Q194207","display_name":"Ethylene glycol","level":2,"score":0.6503371000289917},{"id":"https://openalex.org/C54400483","wikidata":"https://www.wikidata.org/wiki/Q1793202","display_name":"PEG ratio","level":2,"score":0.6449366807937622},{"id":"https://openalex.org/C103038307","wikidata":"https://www.wikidata.org/wiki/Q6556360","display_name":"Lipid bilayer fusion","level":3,"score":0.6216005682945251},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5564207434654236},{"id":"https://openalex.org/C158525013","wikidata":"https://www.wikidata.org/wiki/Q2593739","display_name":"Fusion","level":2,"score":0.44609925150871277},{"id":"https://openalex.org/C2776330855","wikidata":"https://www.wikidata.org/wiki/Q650187","display_name":"Phosphatidylcholine","level":4,"score":0.38223400712013245},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.34177836775779724},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3279169201850891},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.2741689682006836},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.24398145079612732},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.18641608953475952},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.05711793899536133},{"id":"https://openalex.org/C10138342","wikidata":"https://www.wikidata.org/wiki/Q43015","display_name":"Finance","level":1,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008051","descriptor_name":"Lipid Bilayers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008055","descriptor_name":"Lipids","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008081","descriptor_name":"Liposomes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008561","descriptor_name":"Membrane Fusion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010714","descriptor_name":"Phosphatidylethanolamines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010718","descriptor_name":"Phosphatidylserines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016503","descriptor_name":"Drug Delivery Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi952000v","doi":"https://doi.org/10.1021/bi952000v","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi952000v","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:8611565","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8611565","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1597997684","https://openalex.org/W1976319018","https://openalex.org/W2005556449","https://openalex.org/W2032369546","https://openalex.org/W2042968560","https://openalex.org/W2058025744","https://openalex.org/W2060896787","https://openalex.org/W2068971025","https://openalex.org/W2153435210","https://openalex.org/W4400953142"],"related_works":["https://openalex.org/W1975874642","https://openalex.org/W1968537945","https://openalex.org/W2135630168","https://openalex.org/W2080935956","https://openalex.org/W2032671860","https://openalex.org/W1987951093","https://openalex.org/W2011770185","https://openalex.org/W2424693883","https://openalex.org/W2010191807","https://openalex.org/W2029298237"],"abstract_inverted_index":{"The":[0,31,90,156,202],"effect":[1],"of":[2,13,21,33,40,48,63,69,77,81,92,103,116,118,134,152,159,162,167,169,181,189,200,204,212],"poly(ethylene":[3],"glycol)--lipid":[4],"(PEG--lipid)":[5],"conjugates":[6],"on":[7,60,98],"liposomal":[8,174,214],"fusion":[9,93,151,163,175],"was":[10,88,164],"investigated.":[11],"Incorporation":[12],"PEG--lipids":[14],"into":[15],"large":[16,79],"unilamellar":[17],"vesicles":[18,110],"(LUVs)":[19],"composed":[20,123],"equimolar":[22],"phosphatidylethanolamine":[23],"(PE)":[24],"and":[25,44,71,111,114,149],"phosphatidylserine":[26],"(PS)":[27],"inhibited":[28],"calcium-induced":[29],"fusion.":[30,201],"degree":[32,115],"inhibition":[34],"increased":[35],"with":[36,45,131,207],"increasing":[37,46,186],"molar":[38,101],"ratio":[39,102],"the":[41,49,57,61,64,75,99,104,108,112,119,124,135,141,146,153,160,165,170,187,190,198,210],"PEG":[42,50,105,191],"conjugate":[43,106],"size":[47,188],"moiety.":[51],"Inhibition":[52],"appeared":[53],"to":[54,140,196,209],"result":[55],"from":[56,137],"steric":[58,147],"barrier":[59,148],"surface":[62],"liposomes":[65],"which":[66,122],"opposed":[67],"apposition":[68],"bilayers":[70],"interbilayer":[72],"contact.":[73],"In":[74],"presence":[76],"a":[78],"excess":[80],"neutral":[82,142],"acceptor":[83],"liposomes,":[84],"however,":[85],"fusogenic":[86,213],"activity":[87],"restored.":[89],"rate":[91,161,166,199],"under":[94],"these":[95,205],"conditions":[96],"depended":[97],"initial":[100],"in":[107],"PE:PS":[109,138,154],"length":[113],"saturation":[117],"acyl":[120],"chains":[121],"lipid":[125,143],"anchor.":[126],"These":[127],"results":[128,206],"are":[129,218],"consistent":[130],"spontaneous":[132],"transfer":[133,168],"PEG--lipid":[136,183],"LUVs":[139],"sink":[144],"reducing":[145],"allowing":[150],"LUVs.":[155],"primary":[157],"determinant":[158],"PEG--lipid,":[171],"indicating":[172],"that":[173],"could":[176],"be":[177],"programmed":[178],"by":[179],"incorporation":[180],"appropriate":[182],"conjugates.":[184],"Interestingly,":[185],"group":[192],"did":[193],"not":[194],"appear":[195],"affect":[197],"implications":[203],"respect":[208],"design":[211],"drug":[215],"delivery":[216],"systems":[217],"discussed.":[219]},"counts_by_year":[{"year":2026,"cited_by_count":8},{"year":2025,"cited_by_count":11},{"year":2024,"cited_by_count":14},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":12},{"year":2012,"cited_by_count":15}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}