{"id":"https://openalex.org/W1980840949","doi":"https://doi.org/10.1074/jbc.m008512200","title":"Uridine Phosphorylase Association with Vimentin","display_name":"Uridine Phosphorylase Association with Vimentin","publication_year":2001,"publication_date":"2001-04-01","ids":{"openalex":"https://openalex.org/W1980840949","doi":"https://doi.org/10.1074/jbc.m008512200","mag":"1980840949","pmid":"https://pubmed.ncbi.nlm.nih.gov/11278417"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m008512200","doi":"https://doi.org/10.1074/jbc.m008512200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m008512200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m008512200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008022238","display_name":"Rosalind L. Russell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rosalind L. Russell","raw_affiliation_strings":["Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520, USA","From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520, USA","institution_ids":["https://openalex.org/I32971472"]},{"raw_affiliation_string":"From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101072180","display_name":"Deliang Cao","orcid":"https://orcid.org/0009-0006-1578-5738","observed_orcids":["https://orcid.org/0009-0006-1578-5738"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Deliang Cao","raw_affiliation_strings":["From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103207572","display_name":"Dekai Zhang","orcid":"https://orcid.org/0000-0002-9631-5386","observed_orcids":["https://orcid.org/0000-0002-9631-5386"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dekai Zhang","raw_affiliation_strings":["From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041941916","display_name":"Robert E. Handschumacher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert E. Handschumacher","raw_affiliation_strings":["From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016722881","display_name":"Giuseppe Pizzorno","orcid":"https://orcid.org/0000-0001-7087-2806","observed_orcids":["https://orcid.org/0000-0001-7087-2806"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Giuseppe Pizzorno","raw_affiliation_strings":["From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Internal Medicine, Section of Medical Oncology, Yale University School of Medicine, New Haven, Connecticut 06520","institution_ids":["https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5016722881"],"corresponding_institution_ids":["https://openalex.org/I32971472"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.4655,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.58595273,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"276","issue":"16","first_page":"13302","last_page":"13307"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.5967000126838684,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.5967000126838684,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.37790000438690186,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/uridine-phosphorylase","display_name":"uridine phosphorylase","score":0.996999979019165},{"id":"https://openalex.org/keywords/vimentin","display_name":"vimentin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.7870000004768372},{"id":"https://openalex.org/keywords/intermediate-filaments","display_name":"intermediate filaments","score":0.8519999980926514},{"id":"https://openalex.org/keywords/pyrimidine-salvage-pathway","display_name":"pyrimidine salvage pathway","score":0.9120000004768372},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.5049999952316284},{"id":"https://openalex.org/keywords/nocodazole","display_name":"nocodazole","score":0.6959999799728394},{"id":"https://openalex.org/keywords/nih-3t3-cells","display_name":"NIH3T3 cells","score":0.8650000095367432},{"id":"https://openalex.org/keywords/colon-26-cells","display_name":"colon 26 cells","score":0.984000027179718}],"concepts":[{"id":"https://openalex.org/C147447768","wikidata":"https://www.wikidata.org/wiki/Q411624","display_name":"Vimentin","level":3,"score":0.5410711765289307},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46941977739334106},{"id":"https://openalex.org/C142853389","wikidata":"https://www.wikidata.org/wiki/Q744778","display_name":"Association (psychology)","level":2,"score":0.43903839588165283},{"id":"https://openalex.org/C2781259782","wikidata":"https://www.wikidata.org/wiki/Q422573","display_name":"Uridine","level":4,"score":0.4253096580505371},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3795488476753235},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.2550668716430664},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2545279860496521},{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.1941901445388794},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.17154961824417114},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07890057563781738},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.0},{"id":"https://openalex.org/C542102704","wikidata":"https://www.wikidata.org/wiki/Q183257","display_name":"Psychotherapist","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003106","descriptor_name":"Colon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003599","descriptor_name":"Cytoskeleton","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014543","descriptor_name":"Uridine Phosphorylase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D014543","descriptor_name":"Uridine Phosphorylase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014543","descriptor_name":"Uridine Phosphorylase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014746","descriptor_name":"Vimentin","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D014746","descriptor_name":"Vimentin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014746","descriptor_name":"Vimentin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016475","descriptor_name":"3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m008512200","doi":"https://doi.org/10.1074/jbc.m008512200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m008512200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:11278417","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11278417","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m008512200","doi":"https://doi.org/10.1074/jbc.m008512200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m008512200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":71,"referenced_works":["https://openalex.org/W23754806","https://openalex.org/W119252447","https://openalex.org/W223415719","https://openalex.org/W1488605014","https://openalex.org/W1498145857","https://openalex.org/W1532653372","https://openalex.org/W1578172800","https://openalex.org/W1623033053","https://openalex.org/W1662552110","https://openalex.org/W1877106271","https://openalex.org/W1884006126","https://openalex.org/W1966382131","https://openalex.org/W1973822015","https://openalex.org/W1980559044","https://openalex.org/W1981473704","https://openalex.org/W1988866780","https://openalex.org/W1989128266","https://openalex.org/W1990537424","https://openalex.org/W1991681175","https://openalex.org/W2004427424","https://openalex.org/W2011362026","https://openalex.org/W2012355463","https://openalex.org/W2015981605","https://openalex.org/W2016058461","https://openalex.org/W2018343726","https://openalex.org/W2019957079","https://openalex.org/W2032389390","https://openalex.org/W2032584413","https://openalex.org/W2033493540","https://openalex.org/W2034301663","https://openalex.org/W2036235654","https://openalex.org/W2041159818","https://openalex.org/W2045074917","https://openalex.org/W2045381913","https://openalex.org/W2048766593","https://openalex.org/W2049583989","https://openalex.org/W2056327887","https://openalex.org/W2056827417","https://openalex.org/W2057253813","https://openalex.org/W2057635634","https://openalex.org/W2059247762","https://openalex.org/W2059322936","https://openalex.org/W2059408676","https://openalex.org/W2068185510","https://openalex.org/W2072525752","https://openalex.org/W2075533758","https://openalex.org/W2083996759","https://openalex.org/W2088210473","https://openalex.org/W2093314478","https://openalex.org/W2100702185","https://openalex.org/W2114415600","https://openalex.org/W2126048177","https://openalex.org/W2130823501","https://openalex.org/W2130851152","https://openalex.org/W2130867962","https://openalex.org/W2138040506","https://openalex.org/W2139420560","https://openalex.org/W2144724308","https://openalex.org/W2144880676","https://openalex.org/W2157162711","https://openalex.org/W2170057807","https://openalex.org/W2285189681","https://openalex.org/W2314389848","https://openalex.org/W2316437833","https://openalex.org/W2318791783","https://openalex.org/W2411349429","https://openalex.org/W2414312448","https://openalex.org/W2414861445","https://openalex.org/W2472282337","https://openalex.org/W3215454188","https://openalex.org/W4213183351"],"related_works":["https://openalex.org/W2048848497","https://openalex.org/W2079584070","https://openalex.org/W2052090797","https://openalex.org/W2098489231","https://openalex.org/W2119465955","https://openalex.org/W1565982656","https://openalex.org/W7096922589","https://openalex.org/W7098046462","https://openalex.org/W2170210943","https://openalex.org/W7099643795"],"abstract_inverted_index":{"Uridine":[0],"phosphorylase":[1,44],"(UPase),":[2],"a":[3,76,104,172,192],"key":[4],"enzyme":[5],"in":[6,19,95,117,135,168],"the":[7,14,42,129,140,154,164,169,210,220],"pyrimidine":[8],"salvage":[9],"pathway,":[10],"is":[11,113,150,215],"associated":[12,114,138,151,190,216],"with":[13,55,97,115,139,152,191,217],"intermediate":[15,142],"filament":[16],"protein":[17],"vimentin,":[18],"NIH":[20,38,119,178],"3T3":[21,39,120,179],"fibroblasts":[22],"and":[23,37,64,83,88,103,121,125,156,219],"colon":[24,35,122,181],"26":[25,36,123,182],"cells.":[26],"Affinity":[27],"chromatography":[28,74],"was":[29,57],"utilized":[30],"to":[31,49,93,203],"purify":[32],"UPase":[33,56,82,87,112,136,149,166,188,199,214],"from":[34],"cells":[40,124,183],"using":[41,59,71,132],"uridine":[43],"inhibitor":[45],"5'-amino":[46],"benzylacyclouridine":[47],"linked":[48],"an":[50,98,185,223],"agarose":[51],"matrix.":[52],"Vimentin":[53],"copurification":[54],"confirmed":[58],"both":[60,118,153],"Western":[61],"blot":[62],"analysis":[63],"MALDI-MS":[65],"methods.":[66],"Separation":[67],"of":[68,100,106,128,159,163,177,198,222],"cytosolic":[69],"proteins":[70],"gel":[72],"filtration":[73],"yields":[75],"high":[77],"molecular":[78],"weight":[79],"complex":[80],"containing":[81],"vimentin.":[84,144,160],"Purified":[85],"recombinant":[86,89],"vimentin":[90,116,218],"were":[91],"shown":[92,202],"bind":[94],"vitro":[96],"affinity":[99],"120":[101],"pm":[102],"stoichiometry":[105],"1:2.":[107],"Immunofluorescence":[108],"techniques":[109],"confirm":[110],"that":[111,126,148,213],"depolymerization":[127],"microtubule":[130],"system":[131],"nocodazole":[133],"results":[134],"remaining":[137],"collapsed":[141],"filament,":[143],"Our":[145],"data":[146],"demonstrate":[147,208],"soluble":[155,173],"insoluble":[157],"pools":[158,197],"Approximately":[161],"60-70%":[162],"total":[165],"exists":[167],"cytosol":[170],"as":[171],"protein.":[174],"Sequential":[175],"extraction":[176],"or":[180],"liberates":[184],"additional":[186],"30-40%":[187],"activity":[189],"detergent":[193],"extractable":[194],"fraction.":[195],"All":[196],"have":[200],"been":[201],"possess":[204],"enzymatic":[205],"activity.":[206],"We":[207],"for":[209],"first":[211],"time":[212],"existence":[221],"enzymatically":[224],"active":[225],"cytoskeleton-associated":[226],"UPase.":[227]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}