{"id":"https://openalex.org/W1980834856","doi":"https://doi.org/10.3382/ps.2007-00434","title":"An Economic and Simple Purification Procedure for the Large-Scale Production of Ovotransferrin from Egg White","display_name":"An Economic and Simple Purification Procedure for the Large-Scale Production of Ovotransferrin from Egg White","publication_year":2008,"publication_date":"2008-06-24","ids":{"openalex":"https://openalex.org/W1980834856","doi":"https://doi.org/10.3382/ps.2007-00434","mag":"1980834856","pmid":"https://pubmed.ncbi.nlm.nih.gov/18577628"},"language":"en","primary_location":{"id":"doi:10.3382/ps.2007-00434","doi":"https://doi.org/10.3382/ps.2007-00434","is_oa":true,"landing_page_url":"https://doi.org/10.3382/ps.2007-00434","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0032579119400527/pdf","source":{"id":"https://openalex.org/S133896805","display_name":"Poultry Science","issn_l":"0032-5791","issn":["0032-5791","1525-3171"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://www.sciencedirect.com/science/article/pii/S0032579119400527/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084031296","display_name":"Kyung Yuk Ko","orcid":"https://orcid.org/0000-0001-9139-7354","observed_orcids":["https://orcid.org/0000-0001-9139-7354"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K.Y. Ko","raw_affiliation_strings":["Department of Animal Science, Iowa State University, Ames, Iowa 50011-3150, USA","#N#\u2020Department of Animal Science, Iowa State University, Ames 50011-3150"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Science, Iowa State University, Ames, Iowa 50011-3150, USA","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"#N#\u2020Department of Animal Science, Iowa State University, Ames 50011-3150","institution_ids":["https://openalex.org/I173911158"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032945914","display_name":"Dong Uk Ahn","orcid":"https://orcid.org/0000-0001-9523-7630","observed_orcids":["https://orcid.org/0000-0001-9523-7630"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"D.U. Ahn","raw_affiliation_strings":["Department of Animal Science, Iowa State University, Ames, 50011-3150","#N#\u2020Department of Animal Science, Iowa State University, Ames 50011-3150"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Science, Iowa State University, Ames, 50011-3150","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"#N#\u2020Department of Animal Science, Iowa State University, Ames 50011-3150","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5032945914"],"corresponding_institution_ids":["https://openalex.org/I173911158"],"apc_list":{"value":2600,"currency":"USD","value_usd":2600},"apc_paid":{"value":2600,"currency":"USD","value_usd":2600},"fwci":0.448,"has_fulltext":true,"cited_by_count":53,"citation_normalized_percentile":{"value":0.56476347,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"87","issue":"7","first_page":"1441","last_page":"1450"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8384000062942505,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8384000062942505,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10411","display_name":"Proteins in Food Systems","score":0.06960000097751617,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10452","display_name":"Iron Metabolism and Disorders","score":0.026499999687075615,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ovotransferrin","display_name":"ovotransferrin","score":1.0},{"id":"https://openalex.org/keywords/egg-white","display_name":"egg white","score":0.8679999709129333},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.765999972820282},{"id":"https://openalex.org/keywords/iron-binding","display_name":"iron binding","score":0.7829999923706055},{"id":"https://openalex.org/keywords/ethanol-precipitation","display_name":"ethanol precipitation","score":0.9319999814033508},{"id":"https://openalex.org/keywords/iron-removal","display_name":"iron removal","score":0.5239999890327454},{"id":"https://openalex.org/keywords/mass-production","display_name":"mass production","score":0.671999990940094},{"id":"https://openalex.org/keywords/ovalbumin","display_name":"ovalbumin","score":0.5730000138282776},{"id":"https://openalex.org/keywords/iron-binding-proteins","display_name":"iron-binding proteins","score":0.3409999907016754}],"concepts":[{"id":"https://openalex.org/C2780223390","wikidata":"https://www.wikidata.org/wiki/Q7114204","display_name":"Ovotransferrin","level":3,"score":0.9992823600769043},{"id":"https://openalex.org/C2778484053","wikidata":"https://www.wikidata.org/wiki/Q796758","display_name":"Egg white","level":2,"score":0.8518526554107666},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8372818231582642},{"id":"https://openalex.org/C2779429693","wikidata":"https://www.wikidata.org/wiki/Q274959","display_name":"Distilled water","level":2,"score":0.5957134962081909},{"id":"https://openalex.org/C2776946954","wikidata":"https://www.wikidata.org/wiki/Q420291","display_name":"Ovalbumin","level":3,"score":0.570845365524292},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.49913930892944336},{"id":"https://openalex.org/C2780161600","wikidata":"https://www.wikidata.org/wiki/Q153","display_name":"Ethanol","level":2,"score":0.4966591000556946},{"id":"https://openalex.org/C2781321622","wikidata":"https://www.wikidata.org/wiki/Q181409","display_name":"Yolk","level":2,"score":0.4659505784511566},{"id":"https://openalex.org/C2779032678","wikidata":"https://www.wikidata.org/wiki/Q159683","display_name":"Citric acid","level":2,"score":0.4139721989631653},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29368001222610474},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.22908979654312134},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.05278840661048889},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002712","descriptor_name":"Chlorides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003207","descriptor_name":"Conalbumin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003207","descriptor_name":"Conalbumin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004529","descriptor_name":"Egg White","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005290","descriptor_name":"Ferric Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007501","descriptor_name":"Iron","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007501","descriptor_name":"Iron","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012117","descriptor_name":"Resins, Synthetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3382/ps.2007-00434","doi":"https://doi.org/10.3382/ps.2007-00434","is_oa":true,"landing_page_url":"https://doi.org/10.3382/ps.2007-00434","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0032579119400527/pdf","source":{"id":"https://openalex.org/S133896805","display_name":"Poultry Science","issn_l":"0032-5791","issn":["0032-5791","1525-3171"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry Science","raw_type":"journal-article"},{"id":"pmid:18577628","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18577628","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry science","raw_type":"Journal Article"},{"id":"pmh:oai:lib.dr.iastate.edu:ans_pubs-1898","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1898&context=ans_pubs","pdf_url":"https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1898&context=ans_pubs","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Animal Science Publications","raw_type":"text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.1004.9297","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1004.9297","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://ps.oxfordjournals.org/content/87/7/1441.full.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.3382/ps.2007-00434","doi":"https://doi.org/10.3382/ps.2007-00434","is_oa":true,"landing_page_url":"https://doi.org/10.3382/ps.2007-00434","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0032579119400527/pdf","source":{"id":"https://openalex.org/S133896805","display_name":"Poultry Science","issn_l":"0032-5791","issn":["0032-5791","1525-3171"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry Science","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.6735000014305115}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1980834856.pdf","grobid_xml":"https://content.openalex.org/works/W1980834856.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W82958360","https://openalex.org/W152859335","https://openalex.org/W1239058172","https://openalex.org/W1498703985","https://openalex.org/W1512254479","https://openalex.org/W1527627560","https://openalex.org/W1541445884","https://openalex.org/W1571052291","https://openalex.org/W1919234449","https://openalex.org/W1940669864","https://openalex.org/W1965363077","https://openalex.org/W1980109145","https://openalex.org/W1999368492","https://openalex.org/W2019005560","https://openalex.org/W2021555268","https://openalex.org/W2022182574","https://openalex.org/W2045076300","https://openalex.org/W2052781228","https://openalex.org/W2055412584","https://openalex.org/W2055852963","https://openalex.org/W2083305765","https://openalex.org/W2088907594","https://openalex.org/W2091097724","https://openalex.org/W2092292898","https://openalex.org/W2133710714","https://openalex.org/W2323588503","https://openalex.org/W2325564942","https://openalex.org/W2330449262","https://openalex.org/W2344346124","https://openalex.org/W2411811260","https://openalex.org/W2418672971","https://openalex.org/W3158685183","https://openalex.org/W4318443876","https://openalex.org/W6777006456"],"related_works":["https://openalex.org/W7097286180","https://openalex.org/W2029736807","https://openalex.org/W2088958725","https://openalex.org/W2366459532","https://openalex.org/W2055852963","https://openalex.org/W2371325880","https://openalex.org/W2331743256","https://openalex.org/W2358919996","https://openalex.org/W2075500219","https://openalex.org/W345191616"],"abstract_inverted_index":{"The":[0,63,97,188,225,240],"objective":[1],"of":[2,32,65,109,150,170,252],"this":[3,229,273,287],"study":[4],"was":[5,21,47,68,100,180,191,207,231],"to":[6,49,59,70,82,91,95,112,165,209,213,218],"develop":[7],"a":[8,249],"simple":[9],"and":[10,26,76,78,85,119,196,235,246,283,292],"economical":[11],"protocol":[12,230,241],"for":[13,127,177,248],"separating":[14,128],"ovotransferrin":[15,42,99,130,153,179,214,253,260],"from":[16,23,102,131,254],"egg":[17,45,66,104,133,138,255],"white.":[18,256],"Egg":[19],"white":[20,46,67,105,134,139],"separated":[22,101],"the":[24,29,39,103,124,151,156,160,168,174,182,258,268,278],"yolk":[25],"diluted":[27],"with":[28,193],"same":[30],"volume":[31],"distilled":[33,194],"water.":[34],"To":[35],"prevent":[36],"denaturation":[37],"during":[38],"separation":[40],"process,":[41],"in":[43,155,159,205,233,238,264,272,286],"2x-diluted":[44,132],"converted":[48],"its":[50],"holo-form":[51],"by":[52],"adding":[53],"20":[54],"mM":[55,84,222],"FeCl3.6H2O":[56],"solution":[57,163],"(0.25":[58],"3":[60],"mL/100":[61],"mL).":[62],"pH":[64,71,120,216],"adjusted":[69],"7.0,":[72],"8.0,":[73],"or":[74],"9.0,":[75],"NaHCO3":[77],"NaCl":[79],"were":[80,123,143],"added":[81],"50":[83],"0.15":[86],"M,":[87],"respectively":[88],"(final":[89,117],"concentrations)":[90],"facilitate":[92],"iron":[93,203,211],"binding":[94],"ovotransferrin.":[96],"iron-bound":[98,129,152],"using":[106,220,228],"different":[107],"concentrations":[108],"ethanol":[110,162,171,183,284],"(30":[111],"50%).":[113],"Ethanol":[114],"at":[115,121,145],"43%":[116,146,161],"concentration)":[118],"9.0":[122],"best":[125],"conditions":[126],"solution.":[135],"Almost":[136],"all":[137],"proteins":[140],"including":[141],"ovalbumin":[142],"precipitated":[144,189],"ethanol,":[147],"but":[148,173],"most":[149],"remained":[154],"supernatant.":[157],"Holo-ovotransferrin":[158],"started":[164],"precipitate":[166],"as":[167],"concentration":[169,184],"increased,":[172],"optimal":[175],"condition":[176],"precipitating":[178],"when":[181],"reached":[185],"59%":[186],"(final).":[187],"holo-ovotransferrin":[190],"dissolved":[192],"water,":[195],"AG1-X(2)":[197],"ion":[198,280],"exchange":[199,281],"resin":[200,282],"(at":[201],"3x":[202],"content":[204],"ovotransferrin)":[206],"used":[208,271,285],"remove":[210],"bound":[212],"after":[215],"adjustment":[217],"4.7":[219],"500":[221],"citric":[223],"acid.":[224],"apo-ovotransferrin":[226],"obtained":[227],">80%":[232],"purity":[234],"around":[236],"99%":[237],"yield.":[239],"developed":[242],"is":[243,275],"simple,":[244],"economical,":[245],"appropriate":[247],"large-scale":[250],"production":[251],"Also,":[257],"isolated":[259],"can":[261,289],"be":[262,290],"applied":[263],"human":[265],"foods,":[266],"because":[267],"only":[269],"solvent":[270],"process":[274,288],"ethanol.":[276],"Furthermore,":[277],"AG1-X2":[279],"regenerated":[291],"recovered.":[293]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}