{"id":"https://openalex.org/W1980428913","doi":"https://doi.org/10.1021/ja048108s","title":"Precursor-Directed Biosynthesis of Epothilone in Escherichia coli","display_name":"Precursor-Directed Biosynthesis of Epothilone in Escherichia coli","publication_year":2004,"publication_date":"2004-05-26","ids":{"openalex":"https://openalex.org/W1980428913","doi":"https://doi.org/10.1021/ja048108s","mag":"1980428913","pmid":"https://pubmed.ncbi.nlm.nih.gov/15198579"},"language":"en","primary_location":{"id":"doi:10.1021/ja048108s","doi":"https://doi.org/10.1021/ja048108s","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja048108s","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052667915","display_name":"Christopher N. C. Boddy","orcid":"https://orcid.org/0000-0002-3259-3184","observed_orcids":["https://orcid.org/0000-0002-3259-3184"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher N. Boddy","raw_affiliation_strings":["Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026142739","display_name":"Kinya Hotta","orcid":"https://orcid.org/0000-0002-9427-0081","observed_orcids":["https://orcid.org/0000-0002-9427-0081"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kinya Hotta","raw_affiliation_strings":["Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034205295","display_name":"Martha Lovato Tse","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martha Lovato Tse","raw_affiliation_strings":["Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":null,"display_name":"R. Edward Watts","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. Edward Watts","raw_affiliation_strings":["Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038399044","display_name":"Chaitan Khosla","orcid":"https://orcid.org/0000-0001-6529-495X","observed_orcids":["https://orcid.org/0000-0001-6529-495X"]},"institutions":[{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chaitan Khosla","raw_affiliation_strings":["Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Chemical Engineering, Chemistry, and Biochemistry, Stanford University, Stanford, California 94305-5025","institution_ids":["https://openalex.org/I97018004"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I97018004"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":3.9805,"has_fulltext":false,"cited_by_count":66,"citation_normalized_percentile":{"value":0.92936565,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"126","issue":"24","first_page":"7436","last_page":"7437"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.02539999969303608,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/precursor-directed-biosynthesis","display_name":"precursor-directed biosynthesis","score":0.9869999885559082},{"id":"https://openalex.org/keywords/heterologous-biosynthesis","display_name":"heterologous biosynthesis","score":0.8130000233650208},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9620000123977661},{"id":"https://openalex.org/keywords/anticancer-agents","display_name":"anticancer agents","score":0.7310000061988831},{"id":"https://openalex.org/keywords/epothilone-analogues","display_name":"epothilone analogues","score":0.9350000023841858}],"concepts":[{"id":"https://openalex.org/C2777526313","wikidata":"https://www.wikidata.org/wiki/Q408201","display_name":"Epothilone","level":2,"score":0.9029248952865601},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.8075567483901978},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8028327226638794},{"id":"https://openalex.org/C2908737170","wikidata":"https://www.wikidata.org/wiki/Q408201","display_name":"Epothilones","level":2,"score":0.7710756063461304},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7696943283081055},{"id":"https://openalex.org/C2778635478","wikidata":"https://www.wikidata.org/wiki/Q516751","display_name":"Polyketide","level":4,"score":0.5926907658576965},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.5727019906044006},{"id":"https://openalex.org/C114373084","wikidata":"https://www.wikidata.org/wiki/Q273499","display_name":"Chemical synthesis","level":3,"score":0.5597547292709351},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.5465927720069885},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5390267968177795},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5317439436912537},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5286396145820618},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5030121207237244},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.47819116711616516},{"id":"https://openalex.org/C99726746","wikidata":"https://www.wikidata.org/wiki/Q906396","display_name":"Chemical space","level":3,"score":0.4378676414489746},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.4294522702693939},{"id":"https://openalex.org/C2777763344","wikidata":"https://www.wikidata.org/wiki/Q4369963","display_name":"Polyketide synthase","level":5,"score":0.4152156114578247},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3996402621269226},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.3006391227245331},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.2759990096092224},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.23740392923355103},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.1518097221851349},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12950366735458374},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08960080146789551},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000903","descriptor_name":"Antibiotics, Antineoplastic","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000903","descriptor_name":"Antibiotics, Antineoplastic","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D034261","descriptor_name":"Epothilones","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D034261","descriptor_name":"Epothilones","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D034261","descriptor_name":"Epothilones","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/ja048108s","doi":"https://doi.org/10.1021/ja048108s","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja048108s","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:15198579","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15198579","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G418939009","display_name":null,"funder_award_id":"CA-66736","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6202682807","display_name":"Engineered Biosynthesis of Novel Macrolactone Polyketide","funder_award_id":"R01CA066736","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6825484259","display_name":"Combinatorial Biosynthesis of Epothilone Analogs","funder_award_id":"F32CA091636","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1822137126","https://openalex.org/W1966755863","https://openalex.org/W1979687682","https://openalex.org/W1985858069","https://openalex.org/W1990522875","https://openalex.org/W2008336243","https://openalex.org/W2036984750","https://openalex.org/W2053886892","https://openalex.org/W2070892071","https://openalex.org/W2073660800","https://openalex.org/W2076915648","https://openalex.org/W2080312675","https://openalex.org/W2136036783","https://openalex.org/W2161399965","https://openalex.org/W2953306655"],"related_works":["https://openalex.org/W2081228145","https://openalex.org/W2118484018","https://openalex.org/W2083516372","https://openalex.org/W2084636268","https://openalex.org/W2079100753","https://openalex.org/W4237763202","https://openalex.org/W2315354358","https://openalex.org/W2968808954","https://openalex.org/W4230894975","https://openalex.org/W2292408819"],"abstract_inverted_index":{"Engineered":[0],"biosynthetic":[1,28,61,74],"pathways":[2],"provide":[3,133],"a":[4],"powerful":[5],"method":[6],"for":[7,145],"generating":[8],"complex":[9],"molecules.":[10],"Precursor-directed":[11],"biosynthesis,":[12],"which":[13],"combines":[14],"chemical":[15,79,121],"synthesis":[16],"and":[17,65,67,107,130],"enzymatic":[18],"transformations,":[19],"allows":[20],"non-native":[21],"starting":[22],"materials":[23],"to":[24,52,71,101,123,135],"be":[25,124],"incorporated":[26],"into":[27,95,126],"pathways.":[29],"Using":[30],"this":[31,116],"approach,":[32],"we":[33],"achieved":[34],"the":[35,38,54,59,68,73,86,102,127],"production":[36],"of":[37,58],"anticancer":[39],"agent":[40],"epothilone":[41,60,96,136],"C":[42,97],"in":[43,142],"Escherichia":[44],"coli.":[45],"An":[46],"E.":[47,87],"coli":[48,88],"strain":[49,89],"was":[50,76],"engineered":[51],"express":[53],"last":[55],"three":[56],"modules":[57],"pathway":[62],"(epoD-M6,":[63],"epoE,":[64],"epoF)":[66],"substrate":[69,94],"required":[70],"complement":[72],"enzymes":[75],"obtained":[77],"by":[78],"synthesis.":[80],"Under":[81],"high-density":[82],"cell":[83],"culture":[84],"conditions,":[85],"processed":[90],"exogenously":[91],"fed":[92],"synthetic":[93],"at":[98,108],"levels":[99,110],"comparable":[100],"native":[103],"host":[104],"(1":[105],"mg/L)":[106],"higher":[109],"than":[111],"other":[112],"heterologous":[113],"hosts.":[114],"Importantly,":[115],"precursor-directed":[117],"approach":[118],"will":[119],"allow":[120],"modifications":[122],"introduced":[125],"polyketide":[128],"backbone":[129],"may":[131],"ultimately":[132],"access":[134],"analogues":[137],"with":[138],"improved":[139],"pharmacological":[140],"properties":[141],"quantities":[143],"sufficient":[144],"clinical":[146],"development.":[147]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
