{"id":"https://openalex.org/W1980344530","doi":"https://doi.org/10.1016/j.jbiosc.2013.02.020","title":"Improvement of recombinant endoglucanase produced in Pichia pastoris KM71 through the use of synthetic medium for inoculum and pH control of proteolysis","display_name":"Improvement of recombinant endoglucanase produced in Pichia pastoris KM71 through the use of synthetic medium for inoculum and pH control of proteolysis","publication_year":2013,"publication_date":"2013-03-30","ids":{"openalex":"https://openalex.org/W1980344530","doi":"https://doi.org/10.1016/j.jbiosc.2013.02.020","mag":"1980344530","pmid":"https://pubmed.ncbi.nlm.nih.gov/23548272"},"language":"en","primary_location":{"id":"doi:10.1016/j.jbiosc.2013.02.020","doi":"https://doi.org/10.1016/j.jbiosc.2013.02.020","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jbiosc.2013.02.020","pdf_url":null,"source":{"id":"https://openalex.org/S63783143","display_name":"Journal of Bioscience and Bioengineering","issn_l":"1347-4421","issn":["1347-4421","1389-1723"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bioscience and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040606433","display_name":"Theppanya Charoenrat","orcid":"https://orcid.org/0000-0002-9264-3737","observed_orcids":["https://orcid.org/0000-0002-9264-3737"]},"institutions":[{"id":"https://openalex.org/I108108428","display_name":"Thammasat University","ror":"https://ror.org/002yp7f20","country_code":"TH","type":"education","lineage":["https://openalex.org/I108108428"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Theppanya Charoenrat","raw_affiliation_strings":["Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Center, Pathum Thani 12120, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Center, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I108108428"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000756842","display_name":"Nitisarn Khumruaengsri","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I108108428","display_name":"Thammasat University","ror":"https://ror.org/002yp7f20","country_code":"TH","type":"education","lineage":["https://openalex.org/I108108428"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Nitisarn Khumruaengsri","raw_affiliation_strings":["Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Center, Pathum Thani 12120, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Center, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I108108428"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031326716","display_name":"Peerada Promdonkoy","orcid":"https://orcid.org/0000-0002-8568-6464","observed_orcids":["https://orcid.org/0000-0002-8568-6464"]},"institutions":[{"id":"https://openalex.org/I1332092204","display_name":"National Science and Technology Development Agency","ror":"https://ror.org/04vy95b61","country_code":"TH","type":"government","lineage":["https://openalex.org/I1332092204"]},{"id":"https://openalex.org/I902798049","display_name":"National Center for Genetic Engineering and Biotechnology","ror":"https://ror.org/047aswc67","country_code":"TH","type":"facility","lineage":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Peerada Promdonkoy","raw_affiliation_strings":["Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","National Science and Technology Development Agency, Pathum Thani, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]},{"raw_affiliation_string":"National Science and Technology Development Agency, Pathum Thani, Thailand","institution_ids":["https://openalex.org/I1332092204"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062566556","display_name":"Nakul Rattanaphan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1332092204","display_name":"National Science and Technology Development Agency","ror":"https://ror.org/04vy95b61","country_code":"TH","type":"government","lineage":["https://openalex.org/I1332092204"]},{"id":"https://openalex.org/I902798049","display_name":"National Center for Genetic Engineering and Biotechnology","ror":"https://ror.org/047aswc67","country_code":"TH","type":"facility","lineage":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Nakul Rattanaphan","raw_affiliation_strings":["Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","National Science and Technology Development Agency, Pathum Thani, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]},{"raw_affiliation_string":"National Science and Technology Development Agency, Pathum Thani, Thailand","institution_ids":["https://openalex.org/I1332092204"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013974466","display_name":"Lily Eurwilaichitr","orcid":"https://orcid.org/0009-0003-6960-3088","observed_orcids":["https://orcid.org/0009-0003-6960-3088"]},"institutions":[{"id":"https://openalex.org/I1332092204","display_name":"National Science and Technology Development Agency","ror":"https://ror.org/04vy95b61","country_code":"TH","type":"government","lineage":["https://openalex.org/I1332092204"]},{"id":"https://openalex.org/I902798049","display_name":"National Center for Genetic Engineering and Biotechnology","ror":"https://ror.org/047aswc67","country_code":"TH","type":"facility","lineage":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Lily Eurwilaichitr","raw_affiliation_strings":["Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","National Science and Technology Development Agency, Pathum Thani, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]},{"raw_affiliation_string":"National Science and Technology Development Agency, Pathum Thani, Thailand","institution_ids":["https://openalex.org/I1332092204"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075546249","display_name":"Sutipa Tanapongpipat","orcid":"https://orcid.org/0009-0003-7643-2563","observed_orcids":["https://orcid.org/0009-0003-7643-2563"]},"institutions":[{"id":"https://openalex.org/I1332092204","display_name":"National Science and Technology Development Agency","ror":"https://ror.org/04vy95b61","country_code":"TH","type":"government","lineage":["https://openalex.org/I1332092204"]},{"id":"https://openalex.org/I902798049","display_name":"National Center for Genetic Engineering and Biotechnology","ror":"https://ror.org/047aswc67","country_code":"TH","type":"facility","lineage":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Sutipa Tanapongpipat","raw_affiliation_strings":["Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","National Science and Technology Development Agency, Pathum Thani, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]},{"raw_affiliation_string":"National Science and Technology Development Agency, Pathum Thani, Thailand","institution_ids":["https://openalex.org/I1332092204"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028191312","display_name":"Niran Roongsawang","orcid":"https://orcid.org/0000-0002-2114-5052","observed_orcids":["https://orcid.org/0000-0002-2114-5052"]},"institutions":[{"id":"https://openalex.org/I1332092204","display_name":"National Science and Technology Development Agency","ror":"https://ror.org/04vy95b61","country_code":"TH","type":"government","lineage":["https://openalex.org/I1332092204"]},{"id":"https://openalex.org/I902798049","display_name":"National Center for Genetic Engineering and Biotechnology","ror":"https://ror.org/047aswc67","country_code":"TH","type":"facility","lineage":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]}],"countries":["TH"],"is_corresponding":true,"raw_author_name":"Niran Roongsawang","raw_affiliation_strings":["Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","National Science and Technology Development Agency, Pathum Thani, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbial Cell Factory Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand","institution_ids":["https://openalex.org/I1332092204","https://openalex.org/I902798049"]},{"raw_affiliation_string":"National Science and Technology Development Agency, Pathum Thani, Thailand","institution_ids":["https://openalex.org/I1332092204"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5028191312"],"corresponding_institution_ids":["https://openalex.org/I1332092204","https://openalex.org/I902798049"],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":0.8066,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.66464022,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"116","issue":"2","first_page":"193","last_page":"198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9771000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9771000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.021800000220537186,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14438","display_name":"Advanced Scientific Research Methods","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.8730000257492065},{"id":"https://openalex.org/keywords/synthetic-medium","display_name":"synthetic medium","score":0.6539999842643738},{"id":"https://openalex.org/keywords/inoculum-preparation","display_name":"inoculum preparation","score":0.7580000162124634},{"id":"https://openalex.org/keywords/time-delay","display_name":"time delay","score":0.5680000185966492},{"id":"https://openalex.org/keywords/proteolysis","display_name":"proteolysis","score":0.8209999799728394},{"id":"https://openalex.org/keywords/culture-ph","display_name":"culture medium pH","score":0.8510000109672546},{"id":"https://openalex.org/keywords/temperature-effects","display_name":"temperature effects","score":0.48899999260902405},{"id":"https://openalex.org/keywords/specific-enzyme-activity","display_name":"enzyme specific activity","score":0.6299999952316284},{"id":"https://openalex.org/keywords/truncated-protein","display_name":"truncated protein","score":0.6970000267028809},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7839999794960022}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.9698217511177063},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.9010230302810669},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6710264682769775},{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.6288765072822571},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5831578969955444},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5214447379112244},{"id":"https://openalex.org/C2780881558","wikidata":"https://www.wikidata.org/wiki/Q132501","display_name":"Glycerol","level":2,"score":0.47042083740234375},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3515530228614807},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3477393388748169},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.32550865411758423},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002480","descriptor_name":"Cellulase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D002480","descriptor_name":"Cellulase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002480","descriptor_name":"Cellulase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005990","descriptor_name":"Glycerol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D059748","descriptor_name":"Proteolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D061249","descriptor_name":"Batch Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jbiosc.2013.02.020","doi":"https://doi.org/10.1016/j.jbiosc.2013.02.020","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jbiosc.2013.02.020","pdf_url":null,"source":{"id":"https://openalex.org/S63783143","display_name":"Journal of Bioscience and Bioengineering","issn_l":"1347-4421","issn":["1347-4421","1389-1723"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bioscience and Bioengineering","raw_type":"journal-article"},{"id":"pmid:23548272","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23548272","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bioscience and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320336360","display_name":"Scientific Instrument Center, SXU","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W156929183","https://openalex.org/W1595540384","https://openalex.org/W1980139455","https://openalex.org/W1991890196","https://openalex.org/W1995681216","https://openalex.org/W2009674410","https://openalex.org/W2010651809","https://openalex.org/W2017101370","https://openalex.org/W2028875171","https://openalex.org/W2038133864","https://openalex.org/W2051403769","https://openalex.org/W2053803615","https://openalex.org/W2061058657","https://openalex.org/W2082581989","https://openalex.org/W2084671067","https://openalex.org/W2089718635","https://openalex.org/W2094316460","https://openalex.org/W2100837269","https://openalex.org/W2106911235","https://openalex.org/W2115688626","https://openalex.org/W2128635872","https://openalex.org/W2137395761","https://openalex.org/W2147753932","https://openalex.org/W2156702730","https://openalex.org/W2160404128","https://openalex.org/W2171213278","https://openalex.org/W2252731003","https://openalex.org/W2399538424","https://openalex.org/W6781624242"],"related_works":["https://openalex.org/W2153169438","https://openalex.org/W2083243323","https://openalex.org/W4332888","https://openalex.org/W1465686197","https://openalex.org/W2370059666","https://openalex.org/W2565177128","https://openalex.org/W1978664455","https://openalex.org/W2748674589","https://openalex.org/W2179178394","https://openalex.org/W2391567572"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}