{"id":"https://openalex.org/W1980213917","doi":"https://doi.org/10.1002/bit.20220","title":"Dibenzothiophene biodesulfurization pathway improvement using diagnostic GFP fusions","display_name":"Dibenzothiophene biodesulfurization pathway improvement using diagnostic GFP fusions","publication_year":2004,"publication_date":"2004-09-22","ids":{"openalex":"https://openalex.org/W1980213917","doi":"https://doi.org/10.1002/bit.20220","mag":"1980213917","pmid":"https://pubmed.ncbi.nlm.nih.gov/15389482"},"language":"en","primary_location":{"id":"doi:10.1002/bit.20220","doi":"https://doi.org/10.1002/bit.20220","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.20220","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075202545","display_name":"David S. Reichmuth","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David S. Reichmuth","raw_affiliation_strings":["Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA","Department of Chemical Engineering, University of California, Berkeley, CA 94720","fax: +510-643-1228","telephone: +510-642-4862"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA","institution_ids":["https://openalex.org/I95457486"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Berkeley, CA 94720","institution_ids":["https://openalex.org/I95457486"]},{"raw_affiliation_string":"fax: +510-643-1228","institution_ids":[]},{"raw_affiliation_string":"telephone: +510-642-4862","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109919878","display_name":"Harvey W. Blanch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Harvey W. Blanch","raw_affiliation_strings":["Department of Chemical Engineering, University of California, Berkeley, CA 94720; telephone: +510-642-4862; fax: +510-643-1228","Department of Chemical Engineering, University of California, Berkeley, CA 94720","fax: +510-643-1228","telephone: +510-642-4862"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Berkeley, CA 94720; telephone: +510-642-4862; fax: +510-643-1228","institution_ids":["https://openalex.org/I95457486"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Berkeley, CA 94720","institution_ids":["https://openalex.org/I95457486"]},{"raw_affiliation_string":"fax: +510-643-1228","institution_ids":[]},{"raw_affiliation_string":"telephone: +510-642-4862","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000312223","display_name":"Jay D. Keasling","orcid":"https://orcid.org/0000-0003-4170-6088","observed_orcids":["https://orcid.org/0000-0003-4170-6088"]},"institutions":[{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jay D. Keasling","raw_affiliation_strings":["Department of Chemical Engineering, University of California, Berkeley, CA 94720; telephone: +510-642-4862; fax: +510-643-1228","Department of Chemical Engineering, University of California, Berkeley, CA 94720","fax: +510-643-1228","telephone: +510-642-4862"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Berkeley, CA 94720; telephone: +510-642-4862; fax: +510-643-1228","institution_ids":["https://openalex.org/I95457486"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Berkeley, CA 94720","institution_ids":["https://openalex.org/I95457486"]},{"raw_affiliation_string":"fax: +510-643-1228","institution_ids":[]},{"raw_affiliation_string":"telephone: +510-642-4862","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5000312223"],"corresponding_institution_ids":["https://openalex.org/I95457486"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":7.9799,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.96606554,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"88","issue":"1","first_page":"94","last_page":"99"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11351","display_name":"Catalysis and Hydrodesulfurization Studies","score":0.7651000022888184,"subfield":{"id":"https://openalex.org/subfields/2210","display_name":"Mechanical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11351","display_name":"Catalysis and Hydrodesulfurization Studies","score":0.7651000022888184,"subfield":{"id":"https://openalex.org/subfields/2210","display_name":"Mechanical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.14270000159740448,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.03849999979138374,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dibenzothiophene","display_name":"dibenzothiophene","score":0.9120000004768372},{"id":"https://openalex.org/keywords/biodesulfurization","display_name":"biodesulfurization","score":0.9779999852180481},{"id":"https://openalex.org/keywords/5-untranslated-region-mutation","display_name":"5' untranslated region mutation","score":0.6990000009536743},{"id":"https://openalex.org/keywords/translational-fusion","display_name":"translational fusion","score":0.671999990940094},{"id":"https://openalex.org/keywords/gfp-reporter","display_name":"GFP reporter","score":0.753000020980835},{"id":"https://openalex.org/keywords/ribosome-binding-site","display_name":"ribosome binding site","score":0.7900000214576721}],"concepts":[{"id":"https://openalex.org/C2778949310","wikidata":"https://www.wikidata.org/wiki/Q544767","display_name":"Dibenzothiophene","level":3,"score":0.9270428419113159},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6881983280181885},{"id":"https://openalex.org/C68000547","wikidata":"https://www.wikidata.org/wiki/Q903423","display_name":"Flue-gas desulfurization","level":2,"score":0.6429721117019653},{"id":"https://openalex.org/C114847915","wikidata":"https://www.wikidata.org/wiki/Q16948788","display_name":"Ribosomal binding site","level":5,"score":0.6151266694068909},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5688186287879944},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.563111424446106},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.4919995963573456},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46279168128967285},{"id":"https://openalex.org/C518881349","wikidata":"https://www.wikidata.org/wiki/Q682","display_name":"Sulfur","level":2,"score":0.4341011643409729},{"id":"https://openalex.org/C203075996","wikidata":"https://www.wikidata.org/wiki/Q139677","display_name":"Operon","level":4,"score":0.425792932510376},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.25828033685684204},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.25184279680252075},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.10584589838981628}],"mesh":[{"descriptor_ui":"D001673","descriptor_name":"Biodegradation, Environmental","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005579","descriptor_name":"Fossil Fuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013455","descriptor_name":"Sulfur","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013457","descriptor_name":"Sulfur Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013876","descriptor_name":"Thiophenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.20220","doi":"https://doi.org/10.1002/bit.20220","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.20220","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:15389482","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15389482","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.4000000059604645,"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W234408029","https://openalex.org/W1984506228","https://openalex.org/W2008490130","https://openalex.org/W2016814816","https://openalex.org/W2028622989","https://openalex.org/W2059049503","https://openalex.org/W2077374889","https://openalex.org/W2082405411","https://openalex.org/W2087823077","https://openalex.org/W2144634347","https://openalex.org/W2160084442","https://openalex.org/W2314225431","https://openalex.org/W4242612069"],"related_works":["https://openalex.org/W2024423715","https://openalex.org/W4414112064","https://openalex.org/W2007678580","https://openalex.org/W3174155334","https://openalex.org/W7097362144","https://openalex.org/W3174658184","https://openalex.org/W2021879444","https://openalex.org/W4244570100","https://openalex.org/W4406272585","https://openalex.org/W2896938986"],"abstract_inverted_index":{"The":[0],"dibenzothiophene":[1],"biodesulfurization":[2],"pathway":[3],"has":[4],"shown":[5],"significant":[6],"potential":[7],"for":[8],"improving":[9],"the":[10,17,24,28,60,101,105,109,118],"processing":[11],"of":[12,19,46,64,80,111],"sulfur-containing":[13],"fossil":[14],"fuels.":[15],"However,":[16],"rate":[18,110],"desulfurization":[20,112],"is":[21],"limited":[22],"by":[23],"last":[25],"enzyme":[26],"in":[27],"pathway,":[29],"DszB.":[30],"Genetic":[31],"constructs":[32],"designed":[33],"to":[34,43,76,115],"produce":[35],"increased":[36,78,89,95,108],"DszB":[37,58,96],"activity":[38],"were":[39],"not":[40],"functional":[41],"due":[42],"low":[44],"production":[45],"DszB,":[47],"even":[48],"when":[49],"using":[50,68,117],"a":[51],"consensus":[52],"ribosome":[53],"binding":[54],"site.":[55],"To":[56],"increase":[57],"production,":[59],"untranslated":[61],"region":[62],"5'":[63],"dszB":[65,107],"was":[66],"mutated":[67],"degenerate":[69],"oligonucleotides":[70],"and":[71,92],"translational":[72],"fusions":[73],"with":[74,100],"gfp":[75],"detect":[77],"translation":[79],"dszB.":[81,120],"After":[82],"screening":[83],"only":[84],"96":[85],"mutants,":[86],"several":[87],"showed":[88,94],"green":[90],"fluorescence":[91],"two":[93],"activity.":[97],"When":[98],"cotransformed":[99],"full":[102],"dszABC":[103],"operon,":[104],"mutant":[106],"ninefold":[113],"relative":[114],"that":[116],"native":[119]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}