{"id":"https://openalex.org/W1978913089","doi":"https://doi.org/10.1016/j.chroma.2004.10.018","title":"Lysozyme purification from tobacco extract by polyelectrolyte precipitation","display_name":"Lysozyme purification from tobacco extract by polyelectrolyte precipitation","publication_year":2004,"publication_date":"2004-12-15","ids":{"openalex":"https://openalex.org/W1978913089","doi":"https://doi.org/10.1016/j.chroma.2004.10.018","mag":"1978913089","pmid":"https://pubmed.ncbi.nlm.nih.gov/15844488"},"language":"en","primary_location":{"id":"doi:10.1016/j.chroma.2004.10.018","doi":"https://doi.org/10.1016/j.chroma.2004.10.018","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.chroma.2004.10.018","pdf_url":null,"source":{"id":"https://openalex.org/S176136234","display_name":"Journal of Chromatography A","issn_l":"0021-9673","issn":["0021-9673","1873-3778"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography A","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101480352","display_name":"Chenming Zhang","orcid":"https://orcid.org/0000-0002-6770-5334","observed_orcids":["https://orcid.org/0000-0002-6770-5334"]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Chenming Zhang","raw_affiliation_strings":["Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA. cmzhang@vt.edu","Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA. cmzhang@vt.edu","institution_ids":["https://openalex.org/I859038795"]},{"raw_affiliation_string":"Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051580823","display_name":"Raymond Lillie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Raymond Lillie","raw_affiliation_strings":["Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057213848","display_name":"Jackie Cotter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jackie Cotter","raw_affiliation_strings":["Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111758986","display_name":"D. H. Vaughan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David Vaughan","raw_affiliation_strings":["Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210 Seitz Hall, Blacksburg, VA 24061, USA","institution_ids":["https://openalex.org/I859038795"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5101480352"],"corresponding_institution_ids":["https://openalex.org/I859038795"],"apc_list":{"value":4040,"currency":"USD","value_usd":4040},"apc_paid":null,"fwci":1.0415,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.69544995,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"1069","issue":"1","first_page":"107","last_page":"112"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0812000036239624,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11936","display_name":"Chemical and Physical Properties in Aqueous Solutions","score":0.006000000052154064,"subfield":{"id":"https://openalex.org/subfields/1506","display_name":"Filtration and Separation"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lysozyme-purification","display_name":"lysozyme purification","score":0.9559999704360962},{"id":"https://openalex.org/keywords/tobacco-extract","display_name":"tobacco extract","score":0.7990000247955322},{"id":"https://openalex.org/keywords/isoelectric-precipitation","display_name":"isoelectric precipitation","score":0.8629999756813049},{"id":"https://openalex.org/keywords/poly-acrylic-acid","display_name":"poly(acrylic acid)","score":0.843999981880188},{"id":"https://openalex.org/keywords/recombinant-protein-recovery","display_name":"recombinant protein recovery","score":0.781000018119812},{"id":"https://openalex.org/keywords/genetically-modified-plants","display_name":"transgenic plants","score":0.7910000085830688},{"id":"https://openalex.org/keywords/downstream-processing","display_name":"downstream processing","score":0.7089999914169312},{"id":"https://openalex.org/keywords/hydrochloric-acid-precipitation","display_name":"hydrochloric acid precipitation","score":0.7990000247955322},{"id":"https://openalex.org/keywords/polyphenolic-acids","display_name":"polyphenolic acids","score":0.8169999718666077},{"id":"https://openalex.org/keywords/egg-white-lysozyme","display_name":"egg white lysozyme","score":0.9049999713897705}],"concepts":[{"id":"https://openalex.org/C2776966407","wikidata":"https://www.wikidata.org/wiki/Q24779410","display_name":"Lysozyme","level":2,"score":0.9262829422950745},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8769551515579224},{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.7451541423797607},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7351450324058533},{"id":"https://openalex.org/C74774607","wikidata":"https://www.wikidata.org/wiki/Q7251495","display_name":"Protein precipitation","level":3,"score":0.6080666780471802},{"id":"https://openalex.org/C153356533","wikidata":"https://www.wikidata.org/wiki/Q901016","display_name":"Polyelectrolyte","level":3,"score":0.5432880520820618},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.507253110408783},{"id":"https://openalex.org/C2779032678","wikidata":"https://www.wikidata.org/wiki/Q159683","display_name":"Citric acid","level":2,"score":0.4140360355377197},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.3315693736076355},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.302074134349823},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.13918176293373108},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.131547212600708},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.1283802092075348},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004573","descriptor_name":"Electrolytes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007525","descriptor_name":"Isoelectric Focusing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009113","descriptor_name":"Muramidase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.chroma.2004.10.018","doi":"https://doi.org/10.1016/j.chroma.2004.10.018","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.chroma.2004.10.018","pdf_url":null,"source":{"id":"https://openalex.org/S176136234","display_name":"Journal of Chromatography A","issn_l":"0021-9673","issn":["0021-9673","1873-3778"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography A","raw_type":"journal-article"},{"id":"pmid:15844488","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15844488","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of chromatography. A","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W611018878","https://openalex.org/W1964516919","https://openalex.org/W2012043786","https://openalex.org/W2021845043","https://openalex.org/W2057684068","https://openalex.org/W2059703593","https://openalex.org/W2063968553","https://openalex.org/W2071739587","https://openalex.org/W2078470514","https://openalex.org/W2082690070","https://openalex.org/W2106055639","https://openalex.org/W2109975826","https://openalex.org/W4206126480","https://openalex.org/W4232534952"],"related_works":["https://openalex.org/W7099063010","https://openalex.org/W2059703593","https://openalex.org/W2617883007","https://openalex.org/W2082690070","https://openalex.org/W2079109230","https://openalex.org/W2019279823","https://openalex.org/W2360252150","https://openalex.org/W2335624040","https://openalex.org/W1983300832","https://openalex.org/W6925492423"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}