{"id":"https://openalex.org/W1978793389","doi":"https://doi.org/10.1074/jbc.271.40.25019","title":"The Reaction of the Soybean Cotyledon Mitochondrial Cyanide-resistant Oxidase with Sulfhydryl Reagents Suggests That \u03b1-Keto Acid Activation Involves the Formation of a Thiohemiacetal","display_name":"The Reaction of the Soybean Cotyledon Mitochondrial Cyanide-resistant Oxidase with Sulfhydryl Reagents Suggests That \u03b1-Keto Acid Activation Involves the Formation of a Thiohemiacetal","publication_year":1996,"publication_date":"1996-10-01","ids":{"openalex":"https://openalex.org/W1978793389","doi":"https://doi.org/10.1074/jbc.271.40.25019","mag":"1978793389","pmid":"https://pubmed.ncbi.nlm.nih.gov/8798784"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.271.40.25019","doi":"https://doi.org/10.1074/jbc.271.40.25019","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.271.40.25019","pdf_url":"http://www.jbc.org/article/S0021925818401081/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925818401081/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000314919","display_name":"Ann L. Umbach","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ann L. Umbach","raw_affiliation_strings":["Developmental, Cell, and Molecular Biology Group/Botany Department, Duke University, Durham, North Carolina 27708-1000, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Developmental, Cell, and Molecular Biology Group/Botany Department, Duke University, Durham, North Carolina 27708-1000, USA","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109201056","display_name":"James N. Siedow","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"James N. Siedow","raw_affiliation_strings":["Developmental, Cell, and Molecular Biology Group/Botany Department, Duke University, Durham, North Carolina 27708-1000","From the Developmental, Cell, and Molecular Biology Group/Botany Department, Duke University, Durham, North Carolina 27708-1000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Developmental, Cell, and Molecular Biology Group/Botany Department, Duke University, Durham, North Carolina 27708-1000","institution_ids":["https://openalex.org/I170897317"]},{"raw_affiliation_string":"From the Developmental, Cell, and Molecular Biology Group/Botany Department, Duke University, Durham, North Carolina 27708-1000","institution_ids":["https://openalex.org/I170897317"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5109201056"],"corresponding_institution_ids":["https://openalex.org/I170897317"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.8489,"has_fulltext":true,"cited_by_count":81,"citation_normalized_percentile":{"value":0.91830632,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"271","issue":"40","first_page":"25019","last_page":"25026"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9682999849319458,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9682999849319458,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.009700000286102295,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alternative-oxidase","display_name":"alternative oxidase","score":0.8679999709129333},{"id":"https://openalex.org/keywords/pyruvate","display_name":"pyruvate","score":0.9210000038146973},{"id":"https://openalex.org/keywords/sulfhydryl-groups","display_name":"sulfhydryl groups","score":0.8519999980926514},{"id":"https://openalex.org/keywords/iodoacetic-acid","display_name":"iodoacetate","score":0.8870000243186951},{"id":"https://openalex.org/keywords/n-ethylmaleimide","display_name":"N-ethylmaleimide","score":0.9940000176429749},{"id":"https://openalex.org/keywords/disulfide-bonds","display_name":"disulfide bonds","score":0.9300000071525574},{"id":"https://openalex.org/keywords/enzyme-activation-mechanism","display_name":"enzyme activation mechanism","score":0.5809999704360962}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7779693603515625},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5886930823326111},{"id":"https://openalex.org/C2778858093","wikidata":"https://www.wikidata.org/wiki/Q24721080","display_name":"Alternative oxidase","level":3,"score":0.533903956413269},{"id":"https://openalex.org/C38485361","wikidata":"https://www.wikidata.org/wiki/Q576379","display_name":"Oxidase test","level":3,"score":0.5032891631126404},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.47819069027900696},{"id":"https://openalex.org/C2776617069","wikidata":"https://www.wikidata.org/wiki/Q25111100","display_name":"Dithiol","level":2,"score":0.46197664737701416},{"id":"https://openalex.org/C2778455166","wikidata":"https://www.wikidata.org/wiki/Q55076544","display_name":"Cyanide","level":2,"score":0.444691002368927},{"id":"https://openalex.org/C101972281","wikidata":"https://www.wikidata.org/wiki/Q899965","display_name":"Pyruvate decarboxylation","level":4,"score":0.44452497363090515},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.413407564163208},{"id":"https://openalex.org/C88099638","wikidata":"https://www.wikidata.org/wiki/Q412856","display_name":"Pyruvate dehydrogenase complex","level":3,"score":0.3778867721557617},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3739579916000366},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.09764191508293152}],"mesh":[{"descriptor_ui":"D000080","descriptor_name":"Acetals","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005033","descriptor_name":"Ethylmaleimide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007461","descriptor_name":"Iodoacetates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007651","descriptor_name":"Keto Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013025","descriptor_name":"Glycine max","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013439","descriptor_name":"Sulfhydryl Reagents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018548","descriptor_name":"Cotyledon","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D019807","descriptor_name":"Iodoacetic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.271.40.25019","doi":"https://doi.org/10.1074/jbc.271.40.25019","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.271.40.25019","pdf_url":"http://www.jbc.org/article/S0021925818401081/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:8798784","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8798784","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.271.40.25019","doi":"https://doi.org/10.1074/jbc.271.40.25019","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.271.40.25019","pdf_url":"http://www.jbc.org/article/S0021925818401081/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7214595555","display_name":"Studies of the Cyanide-resistant Respiratory Pathway","funder_award_id":"9407759","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1978793389.pdf","grobid_xml":"https://content.openalex.org/works/W1978793389.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1523753595","https://openalex.org/W1539679292","https://openalex.org/W1544546511","https://openalex.org/W1553807233","https://openalex.org/W1561087828","https://openalex.org/W1569506332","https://openalex.org/W1775749144","https://openalex.org/W1866915935","https://openalex.org/W1917100462","https://openalex.org/W1945188237","https://openalex.org/W1955469895","https://openalex.org/W1979961307","https://openalex.org/W1980988729","https://openalex.org/W1991600811","https://openalex.org/W1993000645","https://openalex.org/W1996300552","https://openalex.org/W2003025396","https://openalex.org/W2009098678","https://openalex.org/W2014333268","https://openalex.org/W2020214814","https://openalex.org/W2021065127","https://openalex.org/W2036858892","https://openalex.org/W2046028433","https://openalex.org/W2046981395","https://openalex.org/W2055888671","https://openalex.org/W2056814797","https://openalex.org/W2069293303","https://openalex.org/W2073355465","https://openalex.org/W2078581076","https://openalex.org/W2082915813","https://openalex.org/W2087956118","https://openalex.org/W2091031783","https://openalex.org/W2092248944","https://openalex.org/W2099220890","https://openalex.org/W2123279308","https://openalex.org/W2129111615","https://openalex.org/W2137025662","https://openalex.org/W2147873373","https://openalex.org/W2313129998","https://openalex.org/W2315381002","https://openalex.org/W2322785926","https://openalex.org/W2326762303","https://openalex.org/W2394814575","https://openalex.org/W2950383987","https://openalex.org/W3144316255","https://openalex.org/W4255145826","https://openalex.org/W4300493828"],"related_works":["https://openalex.org/W2062189356","https://openalex.org/W2055888671","https://openalex.org/W2087956118","https://openalex.org/W1986066493","https://openalex.org/W2003038480","https://openalex.org/W2147873373","https://openalex.org/W2039995904","https://openalex.org/W33192424","https://openalex.org/W1866915935","https://openalex.org/W2623279534"],"abstract_inverted_index":{"The":[0,112,141],"cyanide-resistant":[1],"alternative":[2,78,90,138,169,202],"oxidase":[3,79,91,139,170,203],"of":[4,22,31,65,114,120,143,186,194,200,212],"plant":[5],"mitochondria":[6,59],"is":[7,44],"known":[8],"to":[9,48,97,150,167,208],"be":[10,151],"activated":[11],"by":[12,19,123,191,204],"alpha-keto":[13,205],"acids,":[14],"such":[15],"as":[16,83],"pyruvate,":[17,102],"and":[18,76,145],"the":[20,28,32,36,41,63,69,77,105,121,132,137,159,168,172,177,184,187,192,198,201,210],"reduction":[21],"a":[23,84,152,213],"disulfide":[24,71,162],"bond":[25,72],"that":[26,126,156],"bridges":[27],"two":[29],"subunits":[30],"enzyme":[33,43,122],"homodimer.":[34],"When":[35,56],"regulatory":[37,161,179],"cysteines":[38],"are":[39,54],"oxidized,":[40],"inactivated":[42],"much":[45,100],"less":[46],"responsive":[47],"pyruvate":[49,115,144],"than":[50,155],"when":[51,176],"these":[52],"groups":[53],"reduced.":[55],"soybean":[57],"cotyledon":[58],"were":[60,181],"isolated":[61],"in":[62,158],"presence":[64,113],"iodoacetate":[66,94,117,146,165,195],"or":[67,116],"N-ethylmaleimide,":[68,124],"intermolecular":[70],"did":[73],"not":[74],"form":[75],"was":[80,95,148],"present":[81],"only":[82],"noncovalently":[85],"associated":[86],"dimer.":[87],"N-Ethylmaleimide":[88],"inhibited":[89],"activity,":[92],"but":[93],"found":[96],"stimulate":[98],"activity":[99],"like":[101],"including":[103],"enhancing":[104],"enzyme's":[106],"apparent":[107],"affinity":[108],"for":[109],"reduced":[110],"ubiquinone.":[111],"blocked":[118],"inhibition":[119],"indicating":[125],"all":[127],"three":[128],"compounds":[129],"acted":[130],"at":[131,171],"same":[133],"sulfhydryl":[134,154],"group":[135],"on":[136],"protein.":[140],"site":[142,174],"action":[147],"shown":[149],"different":[153],"involved":[157],"redox-active":[160,178],"bond,":[163],"because":[164],"bound":[166],"activating":[173],"even":[175],"sulfhydryls":[180],"oxidized.":[182],"Given":[183],"nature":[185],"covalent":[188],"adduct":[189],"formed":[190],"reaction":[193],"with":[196],"sulfhydryls,":[197],"activation":[199],"acids":[206],"appears":[207],"involve":[209],"formation":[211],"thiohemiacetal.":[214]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}