{"id":"https://openalex.org/W1978749953","doi":"https://doi.org/10.1158/1538-7445.am2013-1116","title":"Abstract 1116: Sleeping Beauty mutagenesis identifies genes and pathways that cooperate with BrafV600E in melanoma initiation and progression.","display_name":"Abstract 1116: Sleeping Beauty mutagenesis identifies genes and pathways that cooperate with BrafV600E in melanoma initiation and progression.","publication_year":2013,"publication_date":"2013-04-01","ids":{"openalex":"https://openalex.org/W1978749953","doi":"https://doi.org/10.1158/1538-7445.am2013-1116","mag":"1978749953"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2013-1116","doi":"https://doi.org/10.1158/1538-7445.am2013-1116","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2013-1116","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000590204","display_name":"Michael B. Mann","orcid":"https://orcid.org/0000-0002-7515-6515","observed_orcids":["https://orcid.org/0000-0002-7515-6515"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael B. Mann","raw_affiliation_strings":["1The Methodist Hospital Research Institute, Houston, TX;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1The Methodist Hospital Research Institute, Houston, TX;","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063087649","display_name":"Michael A. Black","orcid":"https://orcid.org/0000-0003-1174-6054","observed_orcids":["https://orcid.org/0000-0003-1174-6054"]},"institutions":[{"id":"https://openalex.org/I80281795","display_name":"University of Otago","ror":"https://ror.org/01jmxt844","country_code":"NZ","type":"education","lineage":["https://openalex.org/I80281795"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Michael A. Black","raw_affiliation_strings":["2University of Otago, Otago, New Zealand;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2University of Otago, Otago, New Zealand;","institution_ids":["https://openalex.org/I80281795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061909361","display_name":"Jerrold Michael Ward","orcid":"https://orcid.org/0000-0002-6350-8982","observed_orcids":["https://orcid.org/0000-0002-6350-8982"]},"institutions":[{"id":"https://openalex.org/I75573869","display_name":"Institute of Molecular and Cell Biology","ror":"https://ror.org/04xpsrn94","country_code":"SG","type":"facility","lineage":["https://openalex.org/I115228651","https://openalex.org/I2801752549","https://openalex.org/I75573869"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Jerrold M. Ward","raw_affiliation_strings":["3Institute of Molecular and Cell Biology, Singapore, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3Institute of Molecular and Cell Biology, Singapore, Singapore","institution_ids":["https://openalex.org/I75573869"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076813249","display_name":"Marcus Bosenberg","orcid":"https://orcid.org/0000-0003-0166-1612","observed_orcids":["https://orcid.org/0000-0003-0166-1612"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcus W. Bosenberg","raw_affiliation_strings":["4Yale University School of Medicine, New Haven, CT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"4Yale University School of Medicine, New Haven, CT","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003652463","display_name":"Martin McMahon","orcid":"https://orcid.org/0000-0003-2812-1042","observed_orcids":["https://orcid.org/0000-0003-2812-1042"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin McMahon","raw_affiliation_strings":["5University of California, San Francisco, San Francisco, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"5University of California, San Francisco, San Francisco, CA;","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064023142","display_name":"Cristin Gregor Print","orcid":"https://orcid.org/0000-0001-8345-7812","observed_orcids":["https://orcid.org/0000-0001-8345-7812"]},"institutions":[{"id":"https://openalex.org/I154130895","display_name":"University of Auckland","ror":"https://ror.org/03b94tp07","country_code":"NZ","type":"education","lineage":["https://openalex.org/I154130895"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Cristin G. Print","raw_affiliation_strings":["6University of Auckland, Auckland, New Zealand;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"6University of Auckland, Auckland, New Zealand;","institution_ids":["https://openalex.org/I154130895"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102865320","display_name":"David J. Adams","orcid":"https://orcid.org/0000-0001-9490-0306","observed_orcids":["https://orcid.org/0000-0001-9490-0306"]},"institutions":[{"id":"https://openalex.org/I2802476451","display_name":"Wellcome Sanger Institute","ror":"https://ror.org/05cy4wa09","country_code":"GB","type":"nonprofit","lineage":["https://openalex.org/I2802476451","https://openalex.org/I87048295"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"David J. Adams","raw_affiliation_strings":["7Wellcome Trust Sanger Institute, Hinxton, United Kingdom","#N#7Wellcome Trust Sanger Institute, Hinxton, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"7Wellcome Trust Sanger Institute, Hinxton, United Kingdom","institution_ids":["https://openalex.org/I2802476451"]},{"raw_affiliation_string":"#N#7Wellcome Trust Sanger Institute, Hinxton, United Kingdom","institution_ids":["https://openalex.org/I2802476451"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110080713","display_name":"Neal G. Copeland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Neal G. Copeland","raw_affiliation_strings":["1The Methodist Hospital Research Institute, Houston, TX;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1The Methodist Hospital Research Institute, Houston, TX;","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108403260","display_name":"Nancy A. Jenkins","orcid":"https://orcid.org/0009-0005-1155-9314","observed_orcids":["https://orcid.org/0009-0005-1155-9314","https://orcid.org/0000-0003-1653-6574"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nancy A. Jenkins","raw_affiliation_strings":["1The Methodist Hospital Research Institute, Houston, TX;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1The Methodist Hospital Research Institute, Houston, TX;","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]}],"institutions":[],"countries_distinct_count":4,"institutions_distinct_count":8,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"73","issue":"8_Supplement","first_page":"1116","last_page":"1116"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10392","display_name":"Cutaneous Melanoma Detection and Management","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/melanoma","display_name":"melanoma","score":0.9210000038146973},{"id":"https://openalex.org/keywords/braf-v600e-mutation","display_name":"BRAF V600E mutation","score":0.9599999785423279},{"id":"https://openalex.org/keywords/forward-genetic-screen","display_name":"forward genetic screen","score":0.7310000061988831},{"id":"https://openalex.org/keywords/melanoma-progression","display_name":"melanoma progression","score":0.6460000276565552},{"id":"https://openalex.org/keywords/cooperating-mutations","display_name":"cooperating mutations","score":0.628000020980835},{"id":"https://openalex.org/keywords/melanocytes","display_name":"melanocytes","score":0.7580000162124634},{"id":"https://openalex.org/keywords/melanocytic-nevi","display_name":"melanocytic nevi","score":0.8420000076293945},{"id":"https://openalex.org/keywords/cdkn2a","display_name":"CDKN2A","score":0.8880000114440918},{"id":"https://openalex.org/keywords/pten","display_name":"PTEN","score":0.9800000190734863},{"id":"https://openalex.org/keywords/gnaq","display_name":"GNAQ","score":0.9860000014305115},{"id":"https://openalex.org/keywords/mitf","display_name":"MITF","score":0.9929999709129333},{"id":"https://openalex.org/keywords/neurofibromatosis-type-1","display_name":"neurofibromatosis type 1","score":0.9940000176429749},{"id":"https://openalex.org/keywords/rac1","display_name":"Rac1","score":0.9919999837875366},{"id":"https://openalex.org/keywords/ppp6c","display_name":"PPP6C","score":0.9919999837875366},{"id":"https://openalex.org/keywords/wnt-beta-catenin-signaling","display_name":"Wnt/\u03b2-catenin signaling","score":0.9739999771118164},{"id":"https://openalex.org/keywords/tgf-beta-signaling","display_name":"TGF-beta signaling","score":0.9729999899864197},{"id":"https://openalex.org/keywords/pi3k-signaling","display_name":"PI3K signaling","score":0.8930000066757202},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.9860000014305115},{"id":"https://openalex.org/keywords/ubiquitin-mediated-degradation","display_name":"ubiquitin-mediated proteolysis","score":0.9539999961853027},{"id":"https://openalex.org/keywords/tight-junctions","display_name":"tight junctions","score":0.7129999995231628},{"id":"https://openalex.org/keywords/axon-guidance","display_name":"axon guidance","score":0.847000002861023},{"id":"https://openalex.org/keywords/mouse-melanoma-model","display_name":"mouse melanoma model","score":0.6179999709129333}],"concepts":[{"id":"https://openalex.org/C177504595","wikidata":"https://www.wikidata.org/wiki/Q336500","display_name":"Microphthalmia-associated transcription factor","level":4,"score":0.9115372896194458},{"id":"https://openalex.org/C2779176653","wikidata":"https://www.wikidata.org/wiki/Q18026236","display_name":"GNAQ","level":4,"score":0.8584080934524536},{"id":"https://openalex.org/C2777658100","wikidata":"https://www.wikidata.org/wiki/Q180614","display_name":"Melanoma","level":2,"score":0.8278177976608276},{"id":"https://openalex.org/C2780265364","wikidata":"https://www.wikidata.org/wiki/Q5009957","display_name":"CDKN2A","level":3,"score":0.679153323173523},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5806741714477539},{"id":"https://openalex.org/C69991583","wikidata":"https://www.wikidata.org/wiki/Q1423575","display_name":"Candidate gene","level":3,"score":0.5802249908447266},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5798746943473816},{"id":"https://openalex.org/C21790070","wikidata":"https://www.wikidata.org/wiki/Q18030215","display_name":"Neuroblastoma RAS viral oncogene homolog","level":5,"score":0.41816246509552},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.41559910774230957},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3682827949523926},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.2256588339805603},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.0},{"id":"https://openalex.org/C2781187634","wikidata":"https://www.wikidata.org/wiki/Q20969980","display_name":"KRAS","level":4,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2013-1116","doi":"https://doi.org/10.1158/1538-7445.am2013-1116","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2013-1116","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2033175028","https://openalex.org/W4237400193","https://openalex.org/W1970195561","https://openalex.org/W2052510737","https://openalex.org/W1919331313","https://openalex.org/W1991053141","https://openalex.org/W2040944433","https://openalex.org/W2019544036","https://openalex.org/W2109391503","https://openalex.org/W4401428403"],"abstract_inverted_index":{"Abstract":[0],"Malignant":[1],"melanoma":[2,32,70,110,130,139,163,185,219,231,244,302],"is":[3,11,26,35],"a":[4,50,79,223],"devastating":[5],"disease":[6],"the":[7,13,23,42,237,253,310,315],"prevalence":[8],"of":[9,19,44,90,98,114,135,257,309,314],"which":[10],"on":[12],"rise.":[14],"In":[15],"humans,":[16],"somatic":[17],"mutation":[18],"BRAF,":[20],"to":[21,37,58,66,106,108,128,226,252],"encode":[22],"BRAFV600E":[24,99,127],"oncoprotein,":[25],"an":[27,63],"early":[28],"predisposing":[29],"event":[30],"in":[31,41,55,188,301],"initiation,":[33],"but":[34],"insufficient":[36],"drive":[38],"malignant":[39,109],"melanomagenesis":[40],"absence":[43],"additional":[45],"cooperating":[46],"events.":[47],"We":[48],"describe":[49],"genome-wide,":[51],"forward":[52],"genetic":[53],"screen":[54,149],"mice":[56],"restricted":[57],"pigment":[59],"producing":[60],"melanocytes":[61],"as":[62],"unbiased":[64],"approach":[65],"identify":[67],"new":[68,242],"candidate":[69,138,184,230,245],"progression":[71,254],"genes.":[72,232],"By":[73,93],"combining":[74],"Sleeping":[75,290],"Beauty":[76,291],"(SB)":[77],"with":[78,87,126,155,217,248,299],"Cre-activated":[80],"BRafCA":[81],"allele":[82],"we":[83],"observed":[84],"frank":[85],"melanomas":[86],"average":[88],"latency":[89],"36":[91],"weeks.":[92],"contrast,":[94],"melanocyte":[95],"specific":[96],"expression":[97],"elicited":[100],"benign":[101],"melanocytic":[102],"nevi":[103],"that":[104,119,124,158,297],"failed":[105],"progress":[107],"by":[111],"one":[112],"year":[113],"age.":[115],"These":[116],"findings":[117],"confirm":[118],"SB":[120,152],"mobilization":[121],"altered":[122,187],"genes":[123,140,154,186,234,247,294],"cooperate":[125,298],"promote":[129],"progression.":[131,305],"A":[132],"discovery":[133],"set":[134],"1,200":[136],"statistically-defined":[137],"was":[141],"identified":[142,150,235],"from":[143],"70":[144],"SB|BRAFV600E":[145,189,238],"tumors.":[146],"Importantly,":[147],"our":[148],"seven":[151],"mutated":[153,260],"human":[156,162,218,243,258],"orthologs":[157],"are":[159],"well":[160],"established":[161],"drivers":[164],"including:":[165],"Cdkn2a,":[166],"Pten,":[167],"Gnaq,":[168],"Mitf,":[169],"Nf1,":[170],"Rac1":[171],"and":[172,178,195,199,210,228,255,295,304],"Ppp6c.":[173],"Many":[174],"biological":[175,200],"signaling":[176,197],"pathways":[177,198,296],"cellular":[179],"processes":[180,201],"were":[181],"enriched":[182],"for":[183,318],"melanomas,":[190],"including":[191],"Wnt/Beta-catenin,":[192],"TGF\u03b2,":[193],"PI3\u2019-kinase":[194],"MAPK":[196],"regulating":[202],"ubiquitin":[203],"mediated":[204],"proteolysis,":[205],"tight":[206],"junctions,":[207],"cell":[208],"cycle":[209],"axonal":[211],"guidance.":[212],"Integrated":[213],"comparative":[214],"oncogenomic":[215],"analysis":[216],"data":[220],"sets":[221],"provided":[222],"data-driven":[224],"method":[225],"filter":[227],"prioritize":[229],"The":[233],"using":[236],"model":[239],"may":[240],"represent":[241],"cancer":[246],"potential":[249],"clinical":[250],"relevance":[251],"maintenance":[256],"BRAF":[259],"melanoma.":[261],"Citation":[262],"Format:":[263],"Michael":[264,267],"B.":[265],"Mann,":[266],"A.":[268,288],"Black,":[269],"Jerrold":[270],"M.":[271],"Ward,":[272],"Marcus":[273],"W.":[274],"Bosenberg,":[275],"Martin":[276],"McMahon,":[277],"Cristin":[278],"G.":[279,285],"Print,":[280],"David":[281],"J.":[282],"Adams,":[283],"Neal":[284],"Copeland,":[286],"Nancy":[287],"Jenkins.":[289],"mutagenesis":[292],"identifies":[293],"BrafV600E":[300],"initiation":[303],"[abstract].":[306],"In:":[307],"Proceedings":[308],"104th":[311],"Annual":[312],"Meeting":[313],"American":[316],"Association":[317],"Cancer":[319,329],"Research;":[320],"2013":[321],"Apr":[322],"6-10;":[323],"Washington,":[324],"DC.":[325],"Philadelphia":[326],"(PA):":[327],"AACR;":[328],"Res":[330],"2013;73(8":[331],"Suppl):Abstract":[332],"nr":[333],"1116.":[334],"doi:10.1158/1538-7445.AM2013-1116":[335]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}