{"id":"https://openalex.org/W1978278304","doi":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","title":"Use of a DNA toolbox for the characterization of mutation scanning methods. I: Construction of the toolbox and evaluation of heteroduplex analysis","display_name":"Use of a DNA toolbox for the characterization of mutation scanning methods. I: Construction of the toolbox and evaluation of heteroduplex analysis","publication_year":1999,"publication_date":"1999-01-01","ids":{"openalex":"https://openalex.org/W1978278304","doi":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","mag":"1978278304","pmid":"https://pubmed.ncbi.nlm.nih.gov/10380758"},"language":"en","primary_location":{"id":"doi:10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","doi":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000992961","display_name":"William Edward Highsmith","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W. Edward Highsmith","raw_affiliation_strings":["Department of Pathology, University of Maryland Medical School, Baltimore, USA","Department of Pathology, University of Maryland Medical School, Baltimore, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Maryland Medical School, Baltimore, USA","institution_ids":["https://openalex.org/I126744593"]},{"raw_affiliation_string":"Department of Pathology, University of Maryland Medical School, Baltimore, MD, USA","institution_ids":["https://openalex.org/I126744593"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104306708","display_name":"Qian Jin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Qian Jin","raw_affiliation_strings":["Department of Pathology, University of Maryland Medical School, Baltimore, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Maryland Medical School, Baltimore, MD, USA","institution_ids":["https://openalex.org/I126744593"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063137727","display_name":"Arun J. Nataraj","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Arun J. Nataraj","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042853875","display_name":"Jacquelyn M. O'Connor","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jacquelyn M. O'Connor","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080223675","display_name":"Valerie Burland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Valerie D. Burland","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077477617","display_name":"Wendy Baubonis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wendy R. Baubonis","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081421224","display_name":"Foner P. Curtis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Foner P. Curtis","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036992766","display_name":"Noriko Kusukawa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Noriko Kusukawa","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110249545","display_name":"Mark M. Garner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Mark M. Garner","raw_affiliation_strings":["FMC BioProducts, Rockland, ME, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FMC BioProducts, Rockland, ME, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5110249545"],"corresponding_institution_ids":[],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":0.1915,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.39030036,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"20","issue":"6","first_page":"1186","last_page":"1194"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.5160999894142151,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.5160999894142151,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.328900009393692,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.026799999177455902,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heteroduplex-analysis","display_name":"heteroduplex analysis","score":0.8830000162124634},{"id":"https://openalex.org/keywords/mutational-scanning","display_name":"mutation scanning","score":0.8519999980926514},{"id":"https://openalex.org/keywords/single-nucleotide-polymorphisms","display_name":"single nucleotide polymorphisms","score":0.9020000100135803},{"id":"https://openalex.org/keywords/mutation-detection-sensitivity-and-specificity","display_name":"mutation detection sensitivity and specificity","score":0.8040000200271606},{"id":"https://openalex.org/keywords/base-pair-mismatch","display_name":"mismatched base pairs","score":0.746999979019165},{"id":"https://openalex.org/keywords/gc-content","display_name":"GC content","score":0.8140000104904175},{"id":"https://openalex.org/keywords/fragment-length","display_name":"fragment size","score":0.7129999995231628},{"id":"https://openalex.org/keywords/pcr-amplicons","display_name":"PCR amplicons","score":0.5479999780654907}],"concepts":[{"id":"https://openalex.org/C134494468","wikidata":"https://www.wikidata.org/wiki/Q408140","display_name":"Heteroduplex","level":3,"score":0.9824697971343994},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.6377843618392944},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6132485270500183},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.6111700534820557},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5814743638038635},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5507885813713074},{"id":"https://openalex.org/C42058472","wikidata":"https://www.wikidata.org/wiki/Q810214","display_name":"Base (topology)","level":2,"score":0.5404660701751709},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5045298337936401},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.48808753490448},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46621567010879517},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3402770757675171},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17766398191452026},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.13989606499671936},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009692","descriptor_name":"Nucleic Acid Heteroduplexes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","doi":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co;2-6","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"journal-article"},{"id":"doi:10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.3.co;2-y","doi":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.3.co;2-y","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.3.co;2-y","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"journal-article"},{"id":"pmid:10380758","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10380758","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W9587875","https://openalex.org/W290140175","https://openalex.org/W1893714953","https://openalex.org/W1964377753","https://openalex.org/W1967041337","https://openalex.org/W1974467229","https://openalex.org/W1974670959","https://openalex.org/W1978145597","https://openalex.org/W1980312396","https://openalex.org/W1986341687","https://openalex.org/W1987006267","https://openalex.org/W1997935160","https://openalex.org/W2013581852","https://openalex.org/W2019288084","https://openalex.org/W2025948021","https://openalex.org/W2042932259","https://openalex.org/W2045212907","https://openalex.org/W2055985641","https://openalex.org/W2066513477","https://openalex.org/W2066826081","https://openalex.org/W2070524244","https://openalex.org/W2075800046","https://openalex.org/W2079085449","https://openalex.org/W2125650759","https://openalex.org/W2131863464","https://openalex.org/W2137406916","https://openalex.org/W2158295801","https://openalex.org/W2327289992","https://openalex.org/W2613198123","https://openalex.org/W4388384783","https://openalex.org/W4388384803"],"related_works":["https://openalex.org/W1983623378","https://openalex.org/W2077017381","https://openalex.org/W2007254861","https://openalex.org/W4249535064","https://openalex.org/W1981181924","https://openalex.org/W1967885122","https://openalex.org/W1970562783","https://openalex.org/W2089845965","https://openalex.org/W4229835820","https://openalex.org/W2045824760"],"abstract_inverted_index":{"A":[0],"systematic":[1],"characterization":[2],"of":[3,6,15,47,49,84,90,99,115,129,136,143,156,173,181],"the":[4,11,68,85,103,130,137,139,144,154,157,162,166,170,179,182],"effects":[5],"important":[7],"physical":[8],"parameters":[9],"on":[10],"sensitivity":[12],"and":[13,55,120,132,141],"specificity":[14],"methods":[16],"in":[17,82,118,151],"searching":[18],"for":[19,165],"unknown":[20],"base":[21,61,80,105,134,145,159],"changes":[22,62,81],"(mutations":[23],"or":[24,178],"single":[25],"nucleotide":[26],"polymorphisms)":[27],"over":[28],"a":[29,45,64,127],"relatively":[30],"long":[31],"DNA":[32],"segment":[33],"has":[34],"not":[35],"been":[36],"previously":[37],"reported.":[38],"To":[39],"this":[40,88],"end,":[41],"we":[42],"have":[43],"constructed":[44],"set":[46,89],"molecules":[48],"varying":[50],"G+C":[51],"content":[52,135],"(40,":[53],"50,":[54],"60%":[56],"GC)":[57],"having":[58],"all":[59,108],"possible":[60],"at":[63],"particular":[65],"location":[66,142,180],"-":[67],"\"DNA":[69],"toolbox\".":[70],"Exhaustive":[71],"confirmatory":[72],"sequencing":[73],"demonstrated":[74],"that":[75,150],"there":[76],"were":[77,111],"no":[78],"other":[79,109],"any":[83],"clones.":[86],"Using":[87],"clones":[91],"as":[92,126],"polymerase":[93],"chain":[94],"reaction":[95],"(PCR)":[96],"templates,":[97],"amplicons":[98],"various":[100],"lengths":[101],"with":[102],"same":[104],"mutated":[106],"to":[107,168],"bases":[110],"generated.":[112],"The":[113],"behavior":[114],"these":[116],"constructs":[117],"manual":[119],"automated":[121],"heteroduplex":[122,152],"analysis":[123],"was":[124],"analyzed":[125],"function":[128],"size":[131],"overall":[133],"fragment,":[138],"nature":[140,155],"change.":[146],"Our":[147],"results":[148],"show":[149],"analysis,":[153],"mismatched":[158],"pair":[160],"is":[161],"overriding":[163],"determinant":[164],"ability":[167],"detect":[169],"mutation,":[171],"regardless":[172],"fragment":[174],"length,":[175],"GC":[176],"content,":[177],"mutation.":[183]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
