{"id":"https://openalex.org/W1978144288","doi":"https://doi.org/10.1071/is04039","title":"The effects of preservatives and temperatures on arachnid DNA","display_name":"The effects of preservatives and temperatures on arachnid DNA","publication_year":2005,"publication_date":"2005-06-28","ids":{"openalex":"https://openalex.org/W1978144288","doi":"https://doi.org/10.1071/is04039","mag":"1978144288"},"language":"en","primary_location":{"id":"doi:10.1071/is04039","doi":"https://doi.org/10.1071/is04039","is_oa":false,"landing_page_url":"https://doi.org/10.1071/is04039","pdf_url":null,"source":{"id":"https://openalex.org/S190005928","display_name":"Invertebrate Systematics","issn_l":"1445-5226","issn":["1445-5226","1447-2600"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320302","host_organization_name":"CSIRO Publishing","host_organization_lineage":["https://openalex.org/P4310320302"],"host_organization_lineage_names":["CSIRO Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Invertebrate Systematics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085183699","display_name":"Cor J. Vink","orcid":"https://orcid.org/0000-0003-4237-0117","observed_orcids":["https://orcid.org/0000-0003-4237-0117"]},"institutions":[{"id":"https://openalex.org/I26538001","display_name":"San Diego State University","ror":"https://ror.org/0264fdx42","country_code":"US","type":"education","lineage":["https://openalex.org/I26538001"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Cor J. Vink","raw_affiliation_strings":["ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","CCorresponding author. Email: cor.vink@arachnology.org","Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]},{"raw_affiliation_string":"CCorresponding author. Email: cor.vink@arachnology.org","institution_ids":[]},{"raw_affiliation_string":"Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104096566","display_name":"Steven M. Thomas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I26538001","display_name":"San Diego State University","ror":"https://ror.org/0264fdx42","country_code":"US","type":"education","lineage":["https://openalex.org/I26538001"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven M. Thomas","raw_affiliation_strings":["ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]},{"raw_affiliation_string":"Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071285843","display_name":"Pierre Paquin","orcid":"https://orcid.org/0000-0002-2702-311X","observed_orcids":["https://orcid.org/0000-0002-2702-311X"]},"institutions":[{"id":"https://openalex.org/I26538001","display_name":"San Diego State University","ror":"https://ror.org/0264fdx42","country_code":"US","type":"education","lineage":["https://openalex.org/I26538001"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pierre Paquin","raw_affiliation_strings":["ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]},{"raw_affiliation_string":"Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028966867","display_name":"Cheryl Y. Hayashi","orcid":"https://orcid.org/0000-0001-9189-9151","observed_orcids":["https://orcid.org/0000-0001-9189-9151"]},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cheryl Y. Hayashi","raw_affiliation_strings":["BDepartment of Biology, University of California, Riverside, California 92521-0427, USA","Department of Biology, University of California, Riverside, California 92521-0427, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BDepartment of Biology, University of California, Riverside, California 92521-0427, USA","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Department of Biology, University of California, Riverside, California 92521-0427, USA","institution_ids":["https://openalex.org/I103635307"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062127953","display_name":"Marshal C. Hedin","orcid":"https://orcid.org/0000-0001-7683-9572","observed_orcids":["https://orcid.org/0000-0001-7683-9572"]},"institutions":[{"id":"https://openalex.org/I26538001","display_name":"San Diego State University","ror":"https://ror.org/0264fdx42","country_code":"US","type":"education","lineage":["https://openalex.org/I26538001"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marshal Hedin","raw_affiliation_strings":["ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ADepartment of Biology, San Diego State University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]},{"raw_affiliation_string":"Department of Biology, San Diego State  University, San Diego, California 92182-4614, USA","institution_ids":["https://openalex.org/I26538001"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5085183699"],"corresponding_institution_ids":["https://openalex.org/I26538001"],"apc_list":null,"apc_paid":null,"fwci":3.6298,"has_fulltext":false,"cited_by_count":149,"citation_normalized_percentile":{"value":0.92291097,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"19","issue":"2","first_page":"99","last_page":"104"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14399","display_name":"Invertebrate Taxonomy and Ecology","score":0.5800999999046326,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14399","display_name":"Invertebrate Taxonomy and Ecology","score":0.5800999999046326,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.17499999701976776,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12447","display_name":"Spider Taxonomy and Behavior Studies","score":0.09300000220537186,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-preservation","display_name":"DNA preservation","score":0.8679999709129333},{"id":"https://openalex.org/keywords/arachnida","display_name":"Arachnida","score":0.8529999852180481},{"id":"https://openalex.org/keywords/spiders","display_name":"spiders","score":0.9210000038146973},{"id":"https://openalex.org/keywords/scorpions","display_name":"scorpions","score":1.0},{"id":"https://openalex.org/keywords/rnalater","display_name":"RNAlater","score":0.7839999794960022},{"id":"https://openalex.org/keywords/propylene-glycol","display_name":"propylene glycol","score":0.9980000257492065},{"id":"https://openalex.org/keywords/ethanol-storage","display_name":"ethanol storage","score":0.6309999823570251},{"id":"https://openalex.org/keywords/storage-temperature","display_name":"storage temperature","score":0.8209999799728394},{"id":"https://openalex.org/keywords/dna-quality","display_name":"DNA quality","score":0.6549999713897705},{"id":"https://openalex.org/keywords/cytochrome-c-oxidase-subunit-i","display_name":"cytochrome c oxidase subunit I","score":0.8859999775886536},{"id":"https://openalex.org/keywords/actin","display_name":"actin","score":0.8199999928474426},{"id":"https://openalex.org/keywords/pcr-amplification","display_name":"PCR amplification","score":0.5979999899864197},{"id":"https://openalex.org/keywords/museum-specimens","display_name":"museum specimens","score":0.597000002861023}],"concepts":[{"id":"https://openalex.org/C67031304","wikidata":"https://www.wikidata.org/wiki/Q274579","display_name":"Preservative","level":2,"score":0.808335542678833},{"id":"https://openalex.org/C115960442","wikidata":"https://www.wikidata.org/wiki/Q769863","display_name":"Nuclear DNA","level":4,"score":0.5923060178756714},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5487646460533142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.507262647151947},{"id":"https://openalex.org/C24586158","wikidata":"https://www.wikidata.org/wiki/Q27075","display_name":"Mitochondrial DNA","level":3,"score":0.42233896255493164},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.2868974804878235},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22454461455345154},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15100514888763428}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1071/is04039","doi":"https://doi.org/10.1071/is04039","is_oa":false,"landing_page_url":"https://doi.org/10.1071/is04039","pdf_url":null,"source":{"id":"https://openalex.org/S190005928","display_name":"Invertebrate Systematics","issn_l":"1445-5226","issn":["1445-5226","1447-2600"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320302","host_organization_name":"CSIRO Publishing","host_organization_lineage":["https://openalex.org/P4310320302"],"host_organization_lineage_names":["CSIRO Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Invertebrate Systematics","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W305322681","https://openalex.org/W1601722362","https://openalex.org/W1970877575","https://openalex.org/W1983810712","https://openalex.org/W1989705839","https://openalex.org/W1999761959","https://openalex.org/W2028858659","https://openalex.org/W2049567459","https://openalex.org/W2057951383","https://openalex.org/W2064067799","https://openalex.org/W2066207886","https://openalex.org/W2109439286","https://openalex.org/W2112348586","https://openalex.org/W2113962962","https://openalex.org/W2122409447","https://openalex.org/W2140883258","https://openalex.org/W2141718064","https://openalex.org/W2150302133","https://openalex.org/W2162715032","https://openalex.org/W2171847408","https://openalex.org/W2183782875","https://openalex.org/W2231545711","https://openalex.org/W2751179597"],"related_works":["https://openalex.org/W4313388806","https://openalex.org/W1531402601","https://openalex.org/W2124539823","https://openalex.org/W2484300458","https://openalex.org/W1834549221","https://openalex.org/W2359951627","https://openalex.org/W1989175548","https://openalex.org/W2348846577","https://openalex.org/W2037982849","https://openalex.org/W6911036769"],"abstract_inverted_index":{"We":[0,142],"tested":[1],"the":[2,10,29,39,110],"effects":[3],"of":[4,12,50,82],"different":[5,58],"preservatives":[6,40,112,152],"and":[7,14,31,44,65,70,78,92,103,118,153,162],"temperatures":[8,154],"on":[9,76,158],"yield":[11],"spider":[13],"scorpion":[15],"DNA":[16,67,86,96,116],"useable":[17],"for":[18,113,155],"PCR":[19,80],"amplification.":[20],"Our":[21],"experiment":[22],"was":[23,68,72],"designed":[24],"to":[25],"simulate":[26],"conditions":[27],"in":[28,129,150,164],"field":[30],"laboratory":[32],"over":[33],"a":[34],"six-week":[35],"time":[36],"period,":[37],"testing":[38],"RNAlater\u00ae,":[41],"propylene":[42,104],"glycol,":[43],"various":[45],"ethanol":[46,131],"concentrations.":[47],"Three":[48],"replicates":[49],"each":[51],"preservation":[52,117],"treatment":[53],"were":[54],"stored":[55,123,137],"at":[56,124,138],"five":[57],"temperature":[59],"treatments;":[60],"\u201380\u00b0C,":[61],"\u201320\u00b0C,":[62],"2\u20134\u00b0C,":[63],"19\u201324\u00b0C,":[64],"40\u00b0C.":[66],"extracted":[69],"quality":[71,115],"assessed":[73],"by":[74,79],"electrophoresis":[75],"mini-gels,":[77],"amplification":[81],"high":[83,114],"copy":[84,94],"mitochondrial":[85],"fragments":[87,97],"(cytochrome":[88],"oxidase":[89],"subunit":[90],"I)":[91],"low":[93],"nuclear":[95],"(actin).":[98],"Results":[99],"show":[100],"that":[101,119],"RNAlater\u00ae":[102],"glycol":[105],"are":[106,136],"significantly":[107],"better":[108],"than":[109],"other":[111,160],"tissue":[120],"is":[121,132],"best":[122],"\u201380\u00b0C":[125],"or":[126,140],"\u201320\u00b0C.":[127],"Storage":[128],"95%":[130],"appropriate":[133],"if":[134],"specimens":[135],"\u201320\u00b0C":[139],"\u201380\u00b0C.":[141],"believe":[143],"our":[144],"results":[145],"can":[146],"help":[147],"guide":[148],"biologists":[149],"choosing":[151],"DNA-based":[156],"research":[157],"arachnids,":[159],"arthropods":[161],"invertebrates":[163],"general.":[165]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":10},{"year":2016,"cited_by_count":10},{"year":2015,"cited_by_count":16},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":10},{"year":2012,"cited_by_count":8}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}