{"id":"https://openalex.org/W1978110753","doi":"https://doi.org/10.1021/bi011293r","title":"Rapid Evolution of Bacterial Catabolic Enzymes: A Case Study with Atrazine Chlorohydrolase","display_name":"Rapid Evolution of Bacterial Catabolic Enzymes: A Case Study with Atrazine Chlorohydrolase","publication_year":2001,"publication_date":"2001-10-01","ids":{"openalex":"https://openalex.org/W1978110753","doi":"https://doi.org/10.1021/bi011293r","mag":"1978110753","pmid":"https://pubmed.ncbi.nlm.nih.gov/11669610"},"language":"en","primary_location":{"id":"doi:10.1021/bi011293r","doi":"https://doi.org/10.1021/bi011293r","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi011293r","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111603343","display_name":"Jennifer L. Seffernick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennifer L. Seffernick","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology, and Biophysics, Biological Process Technology Institute, and Center for Microbial and Plant Genomics, University of Minnesota, St. Paul, Minnesota 55108"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology, and Biophysics, Biological Process Technology Institute, and Center for Microbial and Plant Genomics, University of Minnesota, St. Paul, Minnesota 55108","institution_ids":["https://openalex.org/I130238516"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054046029","display_name":"Lawrence P. Wackett","orcid":"https://orcid.org/0000-0002-3255-1101","observed_orcids":["https://orcid.org/0000-0002-3255-1101"]},"institutions":[{"id":"https://openalex.org/I130238516","display_name":"University of Minnesota","ror":"https://ror.org/017zqws13","country_code":"US","type":"education","lineage":["https://openalex.org/I130238516"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Lawrence P. Wackett","raw_affiliation_strings":["Department of Biochemistry, Molecular Biology, and Biophysics, Biological Process Technology Institute, and Center for Microbial and Plant Genomics, University of Minnesota, St. Paul, Minnesota 55108"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Molecular Biology, and Biophysics, Biological Process Technology Institute, and Center for Microbial and Plant Genomics, University of Minnesota, St. Paul, Minnesota 55108","institution_ids":["https://openalex.org/I130238516"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5054046029"],"corresponding_institution_ids":["https://openalex.org/I130238516"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.726,"has_fulltext":false,"cited_by_count":86,"citation_normalized_percentile":{"value":0.5,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"40","issue":"43","first_page":"12747","last_page":"12753"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11180","display_name":"Pesticide and Herbicide Environmental Studies","score":0.8827999830245972,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11180","display_name":"Pesticide and Herbicide Environmental Studies","score":0.8827999830245972,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.017799999564886093,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.017400000244379044,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzyme-evolution","display_name":"enzyme evolution","score":0.8650000095367432},{"id":"https://openalex.org/keywords/reductive-dechlorination","display_name":"reductive dechlorination","score":0.9369999766349792},{"id":"https://openalex.org/keywords/herbicide-degradation","display_name":"herbicide degradation","score":0.6890000104904175},{"id":"https://openalex.org/keywords/atrazine","display_name":"atrazine","score":0.578000009059906}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.6382671594619751},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5150875449180603},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.4958990514278412},{"id":"https://openalex.org/C518677369","wikidata":"https://www.wikidata.org/wiki/Q202833","display_name":"Social media","level":2,"score":0.44395753741264343},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.32755550742149353}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000619","descriptor_name":"Aminohydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000619","descriptor_name":"Aminohydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004798","descriptor_name":"Enzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004798","descriptor_name":"Enzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006867","descriptor_name":"Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006867","descriptor_name":"Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014644","descriptor_name":"Genetic Variation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019143","descriptor_name":"Evolution, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi011293r","doi":"https://doi.org/10.1021/bi011293r","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi011293r","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:11669610","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11669610","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3085450798","display_name":null,"funder_award_id":"GM 08347","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W641673757","https://openalex.org/W1552351802","https://openalex.org/W1952433052","https://openalex.org/W2004517409","https://openalex.org/W2018383874","https://openalex.org/W2020088347","https://openalex.org/W2052822239","https://openalex.org/W2089766135","https://openalex.org/W2116802081","https://openalex.org/W2117220506","https://openalex.org/W2158783389","https://openalex.org/W2160216877","https://openalex.org/W4249499016"],"related_works":["https://openalex.org/W2104922640","https://openalex.org/W4245542106","https://openalex.org/W2499107053","https://openalex.org/W2157915912","https://openalex.org/W7096242515","https://openalex.org/W2908240748","https://openalex.org/W2372546450","https://openalex.org/W1468073849","https://openalex.org/W3039683635","https://openalex.org/W2116802081"],"abstract_inverted_index":{"ADVERTISEMENT":[0],"RETURN":[1],"TO":[2],"ISSUECurrent":[3],"Topics/Persp...Current":[4],"Topics/PerspectivesNEXTRapid":[5],"Evolution":[6],"of":[7,26,44,91,126,152],"Bacterial":[8],"Catabolic":[9],"Enzymes:":[10],"A":[11],"Case":[12],"Study":[13],"with":[14,173,208],"Atrazine":[15],"Chlorohydrolase\u2020Jennifer":[16],"L.":[17],"Seffernick":[18],"and":[19,30,36,40,101,106,135,179,196,212,214,225],"Lawrence":[20],"P.":[21],"WackettView":[22],"Author":[23],"Information":[24],"Department":[25],"Biochemistry,":[27],"Molecular":[28],"Biology,":[29],"Biophysics,":[31],"Biological":[32],"Process":[33],"Technology":[34],"Institute,":[35],"Center":[37],"for":[38,184],"Microbial":[39],"Plant":[41],"Genomics,":[42],"University":[43],"Minnesota,":[45],"St.":[46],"Paul,":[47],"Minnesota":[48],"55108":[49],"Cite":[50],"this:":[51],"Biochemistry":[52],"2001,":[53],"40,":[54],"43,":[55],"12747\u201312753Publication":[56],"Date":[57],"(Web):October":[58],"3,":[59],"2001Publication":[60],"History":[61],"Received20":[62],"June":[63],"2001Revised31":[64],"August":[65],"2001Published":[66,69],"online3":[67],"October":[68,72],"inissue":[70],"1":[71],"2001https://pubs.acs.org/doi/10.1021/bi011293rhttps://doi.org/10.1021/bi011293rreview-articleACS":[73],"PublicationsCopyright":[74],"\u00a9":[75],"2001":[76],"American":[77],"Chemical":[78],"SocietyRequest":[79],"reuse":[80],"permissionsArticle":[81],"Views863Altmetric-Citations72LEARN":[82],"ABOUT":[83],"THESE":[84],"METRICSArticle":[85],"Views":[86],"are":[87,110,123],"the":[88,119,124,153,164,177,180,185,192,198],"COUNTER-compliant":[89],"sum":[90],"full":[92],"text":[93],"article":[94,158],"downloads":[95],"since":[96],"November":[97],"2008":[98],"(both":[99],"PDF":[100],"HTML)":[102],"across":[103],"all":[104],"institutions":[105],"individuals.":[107],"These":[108],"metrics":[109],"regularly":[111],"updated":[112,136],"to":[113,118],"reflect":[114],"usage":[115],"leading":[116],"up":[117],"last":[120],"few":[121],"days.Citations":[122],"number":[125],"other":[127],"articles":[128],"citing":[129],"this":[130],"article,":[131],"calculated":[132],"by":[133],"Crossref":[134,142],"daily.":[137],"Find":[138,188],"more":[139,189],"information":[140,190],"about":[141,176],"citation":[143],"counts.The":[144],"Altmetric":[145,193],"Attention":[146,194],"Score":[147,195],"is":[148,200],"a":[149,156,169],"quantitative":[150],"measure":[151],"attention":[154],"that":[155],"research":[157],"has":[159],"received":[160],"online.":[161],"Clicking":[162],"on":[163,191],"donut":[165],"icon":[166],"will":[167],"load":[168],"page":[170],"at":[171],"altmetric.com":[172],"additional":[174],"details":[175],"score":[178,199],"social":[181],"media":[182],"presence":[183],"given":[186],"article.":[187],"how":[197],"calculated.":[201],"Share":[202,217],"Add":[203],"toView":[204],"InAdd":[205],"Full":[206],"Text":[207],"ReferenceAdd":[209],"Description":[210],"ExportRISCitationCitation":[211],"abstractCitation":[213],"referencesMore":[215],"Options":[216],"onFacebookTwitterWechatLinked":[218],"InRedditEmail":[219],"Other":[220],"access":[221],"optionsGet":[222],"e-Alertsclose":[223],"SUBJECTS:Bacteria,Melamine,Peptides":[224],"proteins,Pest":[226],"control,Reductive":[227],"dechlorination":[228],"Get":[229],"e-Alerts":[230]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}