{"id":"https://openalex.org/W1977540748","doi":"https://doi.org/10.1083/jcb.83.3.562","title":"Growth control in cultured 3T3 fibroblasts. Assays of cell proliferation and demonstration of a growth inhibitory activity.","display_name":"Growth control in cultured 3T3 fibroblasts. Assays of cell proliferation and demonstration of a growth inhibitory activity.","publication_year":1979,"publication_date":"1979-12-01","ids":{"openalex":"https://openalex.org/W1977540748","doi":"https://doi.org/10.1083/jcb.83.3.562","mag":"1977540748","pmid":"https://pubmed.ncbi.nlm.nih.gov/521454","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2110507"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.83.3.562","doi":"https://doi.org/10.1083/jcb.83.3.562","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.83.3.562","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2110507","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052687048","display_name":"Peter A. Steck","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P A Steck","raw_affiliation_strings":["Michigan State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Michigan State University","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044849316","display_name":"Patricia G. Voss","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87216513","display_name":"Michigan State University","ror":"https://ror.org/05hs6h993","country_code":"US","type":"education","lineage":["https://openalex.org/I87216513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P G Voss","raw_affiliation_strings":["Michigan State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Michigan State University","institution_ids":["https://openalex.org/I87216513"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025992624","display_name":"J L Wang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J L Wang","raw_affiliation_strings":["Biochemistry and Molecular Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry and Molecular Biology","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":7.3407,"has_fulltext":false,"cited_by_count":67,"citation_normalized_percentile":{"value":0.9704413,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"83","issue":"3","first_page":"562","last_page":"575"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.3756999969482422,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.3756999969482422,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.14509999752044678,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.13760000467300415,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/3t3-cells","display_name":"3T3 fibroblasts","score":0.9210000038146973},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.7229999899864197},{"id":"https://openalex.org/keywords/growth-inhibition","display_name":"growth inhibition","score":0.8479999899864197},{"id":"https://openalex.org/keywords/conditioned-medium","display_name":"conditioned medium","score":0.9779999852180481},{"id":"https://openalex.org/keywords/growth-inhibitory-factor","display_name":"growth inhibitory factor","score":0.777999997138977},{"id":"https://openalex.org/keywords/thymidine-incorporation","display_name":"thymidine incorporation","score":0.7170000076293945},{"id":"https://openalex.org/keywords/cell-cycle","display_name":"cell cycle","score":0.42899999022483826},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6399999856948853},{"id":"https://openalex.org/keywords/serum-free-medium","display_name":"serum-free medium","score":0.621999979019165}],"concepts":[{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.7967039346694946},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7406883239746094},{"id":"https://openalex.org/C2776746612","wikidata":"https://www.wikidata.org/wiki/Q68153243","display_name":"3T3 cells","level":4,"score":0.7053901553153992},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.6314495801925659},{"id":"https://openalex.org/C17077164","wikidata":"https://www.wikidata.org/wiki/Q1185869","display_name":"Inhibitory postsynaptic potential","level":2,"score":0.5875204801559448},{"id":"https://openalex.org/C85813293","wikidata":"https://www.wikidata.org/wiki/Q188909","display_name":"Cell division","level":3,"score":0.5644888877868652},{"id":"https://openalex.org/C64241487","wikidata":"https://www.wikidata.org/wiki/Q5205774","display_name":"DNA synthesis","level":3,"score":0.5616121292114258},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5224810242652893},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.49367818236351013},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4673942029476166},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4561062157154083},{"id":"https://openalex.org/C2780919596","wikidata":"https://www.wikidata.org/wiki/Q575920","display_name":"Growth medium","level":3,"score":0.4512766897678375},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.32568514347076416},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.08033594489097595},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07367292046546936},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.06765320897102356},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002452","descriptor_name":"Cell Count","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006131","descriptor_name":"Growth Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D013936","descriptor_name":"Thymidine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1083/jcb.83.3.562","doi":"https://doi.org/10.1083/jcb.83.3.562","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.83.3.562","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2110507","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2110507","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmid:521454","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/521454","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.280.4778","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4778","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/05/3e/J_Cell_Biol_1982_Feb_1_92(2)_523-530.tar.gz","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2110507","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2110507","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W1527722265","https://openalex.org/W1775749144","https://openalex.org/W1963979467","https://openalex.org/W1964504520","https://openalex.org/W1964784148","https://openalex.org/W1965692713","https://openalex.org/W1969919651","https://openalex.org/W1971182101","https://openalex.org/W1985192626","https://openalex.org/W2002973844","https://openalex.org/W2003424598","https://openalex.org/W2016658627","https://openalex.org/W2018284069","https://openalex.org/W2032889111","https://openalex.org/W2034268599","https://openalex.org/W2038644016","https://openalex.org/W2045934824","https://openalex.org/W2049043712","https://openalex.org/W2052970532","https://openalex.org/W2064331161","https://openalex.org/W2064541581","https://openalex.org/W2064670731","https://openalex.org/W2065618463","https://openalex.org/W2073762409","https://openalex.org/W2078429643","https://openalex.org/W2091928150","https://openalex.org/W2097382077","https://openalex.org/W2112878828","https://openalex.org/W2154214377","https://openalex.org/W2169413646","https://openalex.org/W2329710785"],"related_works":["https://openalex.org/W3045755994","https://openalex.org/W2149659212","https://openalex.org/W2152313739","https://openalex.org/W7099308595","https://openalex.org/W83205721","https://openalex.org/W2030004087","https://openalex.org/W2062751230","https://openalex.org/W2065479052","https://openalex.org/W2020028475","https://openalex.org/W2061838091"],"abstract_inverted_index":{"Treatment":[0],"of":[1,5,23,43,60,66,75,78,87,95,110,121,134,180,207],"sparse,":[2],"proliferating":[3],"cultures":[4,18],"3T3":[6,17],"cells":[7,30,51,84,101],"(target":[8],"cells)":[9],"with":[10,36,176,190],"medium":[11,38,45],"conditioned":[12,44],"by":[13,104,155,160],"exposure":[14],"to":[15,33,173],"density-inhibited":[16],"resulted":[19],"in":[20,27,82,91,97,107,117,126,151,216],"an":[21],"inhibition":[22],"growth":[24,145,208,214],"and":[25,47,72,99,158,204],"division":[26],"the":[28,76,92,108,118,127,131,135,167,177,181,202],"target":[29,50,138],"when":[31],"compared":[32],"similar":[34,188],"treatment":[35,189],"unconditioned":[37],"(UCM).":[39],"This":[40],"differential":[41],"effect":[42,133],"(CM)":[46],"UCM":[48],"on":[49,137],"was":[52,102,114,141,170,185],"demonstrated":[53],"using":[54],"three":[55],"assay":[56],"systems:":[57],"(a)":[58],"assessment":[59],"total":[61,93],"cell":[62,125,139],"number;":[63],"(b)":[64],"measurement":[65],"[3H]thymidine":[67,96],"incorporated":[68],"into":[69],"acid-precipitable":[70],"DNA;":[71],"(c)":[73],"determination":[74],"percentage":[77],"radioactively":[79],"labeled":[80,111,124],"nuclei":[81],"individual":[83],"after":[85],"incorporation":[86,94],"[3H]thymidine.":[88],"The":[89],"difference":[90,106],"CM-treated":[98],"UCM-treated":[100],"reflected":[103],"a":[105,196],"percent":[109],"cells.":[112],"There":[113],"no":[115],"differences":[116],"average":[119],"number":[120],"grains":[122],"per":[123],"two":[128],"cultures.":[129],"Moreover,":[130],"inhibitory":[132,146,168,209],"CM":[136],"proliferation":[140],"reversible.":[142],"Finally,":[143],"this":[144],"activity":[147,169,184],"can":[148],"be":[149,174],"collected":[150],"serum-free":[152],"medium,":[153],"precipitated":[154],"ammonium":[156],"sulfate,":[157],"fractionated":[159],"gel":[161],"filtration.":[162],"In":[163],"these":[164],"purification":[165,203],"procedures,":[166],"consistently":[171],"found":[172,186],"associated":[175],"protein-containing":[178],"fractions":[179],"CM.":[182],"No":[183],"upon":[187],"UCM.":[191],"These":[192],"results":[193],"suggest":[194],"that":[195,211],"system":[197],"has":[198],"been":[199],"developed":[200],"for":[201],"molecular":[205],"analysis":[206],"factors":[210],"may":[212],"mediate":[213],"control":[215],"culture":[217],"fibroblasts.":[218]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}