{"id":"https://openalex.org/W1975282997","doi":"https://doi.org/10.1111/j.1365-2141.1995.tb05145.x","title":"Transcriptional and post\u2010transcriptional regulation of granulocyte\u2010macrophage colony\u2010stimulating factor production in human growth factor dependent M\u201007e cells","display_name":"Transcriptional and post\u2010transcriptional regulation of granulocyte\u2010macrophage colony\u2010stimulating factor production in human growth factor dependent M\u201007e cells","publication_year":1995,"publication_date":"1995-06-01","ids":{"openalex":"https://openalex.org/W1975282997","doi":"https://doi.org/10.1111/j.1365-2141.1995.tb05145.x","mag":"1975282997","pmid":"https://pubmed.ncbi.nlm.nih.gov/7540853"},"language":"en","primary_location":{"id":"doi:10.1111/j.1365-2141.1995.tb05145.x","doi":"https://doi.org/10.1111/j.1365-2141.1995.tb05145.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-2141.1995.tb05145.x","pdf_url":null,"source":{"id":"https://openalex.org/S153015021","display_name":"British Journal of Haematology","issn_l":"0007-1048","issn":["0007-1048","1365-2141"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British Journal of Haematology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111547486","display_name":"M F Brizzi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"MARIA FELICE BRIZZI","raw_affiliation_strings":["Dipartimento di Scienze Biomediche e Oncologia Umana, Universit\u00e0 di Torino, Italy","Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dipartimento di Scienze Biomediche e Oncologia Umana, Universit\u00e0 di Torino, Italy","institution_ids":["https://openalex.org/I55143463"]},{"raw_affiliation_string":"Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066185229","display_name":"Pier Riccardo Rossi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"PIER RICCARDO ROSSI","raw_affiliation_strings":["Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113770386","display_name":"Arturo Rosso","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"ARTURO ROSSO","raw_affiliation_strings":["Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080147501","display_name":"Gian Carlo Avanzi","orcid":"https://orcid.org/0000-0001-7611-8345","observed_orcids":["https://orcid.org/0000-0001-7611-8345"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"GIAN CARLO AVANZI","raw_affiliation_strings":["Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109094873","display_name":"L Pegoraro","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"LOIGI PEGORARO","raw_affiliation_strings":["Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dipartimento di Sclenze Biomediche e Oncologia Umana, Universita di Torino, Torino, Italy","institution_ids":["https://openalex.org/I55143463"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I55143463"],"apc_list":{"value":5190,"currency":"USD","value_usd":5190},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.07995714,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"90","issue":"2","first_page":"258","last_page":"265"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11503","display_name":"Cytokine Signaling Pathways and Interactions","score":0.7002000212669373,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11503","display_name":"Cytokine Signaling Pathways and Interactions","score":0.7002000212669373,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.17319999635219574,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11346","display_name":"Mast cells and histamine","score":0.01769999973475933,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gm-csf","display_name":"GM-CSF","score":0.9890000224113464},{"id":"https://openalex.org/keywords/post-transcriptional-regulation","display_name":"post-transcriptional regulation","score":0.902999997138977},{"id":"https://openalex.org/keywords/interleukin-3","display_name":"interleukin-3","score":0.9950000047683716},{"id":"https://openalex.org/keywords/stem-cell-factor","display_name":"stem cell factor","score":0.9980000257492065},{"id":"https://openalex.org/keywords/gm-csf-secretion","display_name":"GM-CSF secretion","score":0.8769999742507935},{"id":"https://openalex.org/keywords/cycloheximide","display_name":"cycloheximide","score":0.8740000128746033},{"id":"https://openalex.org/keywords/growth-factor-dependence","display_name":"growth factor dependence","score":0.5230000019073486}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.681152880191803},{"id":"https://openalex.org/C2777478134","wikidata":"https://www.wikidata.org/wiki/Q423660","display_name":"Granulocyte macrophage colony-stimulating factor","level":3,"score":0.6783762574195862},{"id":"https://openalex.org/C2778128677","wikidata":"https://www.wikidata.org/wiki/Q412895","display_name":"Cycloheximide","level":3,"score":0.651175320148468},{"id":"https://openalex.org/C2778690821","wikidata":"https://www.wikidata.org/wiki/Q212354","display_name":"Cytokine","level":2,"score":0.503619372844696},{"id":"https://openalex.org/C2775960820","wikidata":"https://www.wikidata.org/wiki/Q408378","display_name":"Growth factor","level":3,"score":0.4682416021823883},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.46176379919052124},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4421420097351074},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.44122856855392456},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4348069727420807},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43291541934013367},{"id":"https://openalex.org/C880424","wikidata":"https://www.wikidata.org/wiki/Q416030","display_name":"Colony-stimulating factor","level":4,"score":0.42218494415283203},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.395555704832077},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.39390289783477783},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3639872670173645},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.337365984916687},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.33192330598831177},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.31101515889167786},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1886376142501831},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.15350815653800964},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1349760890007019},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.11614683270454407},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0658896267414093},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.06258922815322876},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003513","descriptor_name":"Cycloheximide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007377","descriptor_name":"Interleukin-3","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007951","descriptor_name":"Leukemia, Myeloid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012323","descriptor_name":"RNA Processing, Post-Transcriptional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016178","descriptor_name":"Granulocyte-Macrophage Colony-Stimulating Factor","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016178","descriptor_name":"Granulocyte-Macrophage Colony-Stimulating Factor","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016298","descriptor_name":"Hematopoietic Cell Growth Factors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D019089","descriptor_name":"Stem Cell Factor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1365-2141.1995.tb05145.x","doi":"https://doi.org/10.1111/j.1365-2141.1995.tb05145.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-2141.1995.tb05145.x","pdf_url":null,"source":{"id":"https://openalex.org/S153015021","display_name":"British Journal of Haematology","issn_l":"0007-1048","issn":["0007-1048","1365-2141"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British Journal of Haematology","raw_type":"journal-article"},{"id":"pmid:7540853","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7540853","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"British journal of haematology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":50,"referenced_works":["https://openalex.org/W17708317","https://openalex.org/W173994574","https://openalex.org/W180822945","https://openalex.org/W193851687","https://openalex.org/W203102217","https://openalex.org/W207944929","https://openalex.org/W270079318","https://openalex.org/W1501358984","https://openalex.org/W1532659659","https://openalex.org/W1547236510","https://openalex.org/W1547869235","https://openalex.org/W1548222831","https://openalex.org/W1579093659","https://openalex.org/W1589881672","https://openalex.org/W1796692482","https://openalex.org/W1977157092","https://openalex.org/W1987043992","https://openalex.org/W1993604403","https://openalex.org/W2005388055","https://openalex.org/W2006612940","https://openalex.org/W2028395097","https://openalex.org/W2034280067","https://openalex.org/W2053422110","https://openalex.org/W2054997145","https://openalex.org/W2058563741","https://openalex.org/W2062042301","https://openalex.org/W2066804997","https://openalex.org/W2074498234","https://openalex.org/W2077047827","https://openalex.org/W2078055044","https://openalex.org/W2080532845","https://openalex.org/W2097779050","https://openalex.org/W2100564190","https://openalex.org/W2134812217","https://openalex.org/W2147505589","https://openalex.org/W2149294505","https://openalex.org/W2368691364","https://openalex.org/W2395259494","https://openalex.org/W2395321879","https://openalex.org/W2402206772","https://openalex.org/W2402720811","https://openalex.org/W2405878472","https://openalex.org/W2406897504","https://openalex.org/W2410687897","https://openalex.org/W2413648258","https://openalex.org/W2415966257","https://openalex.org/W2425853432","https://openalex.org/W2463577369","https://openalex.org/W2473170182","https://openalex.org/W4300854755"],"related_works":["https://openalex.org/W109921148","https://openalex.org/W1980378275","https://openalex.org/W2418469641","https://openalex.org/W1699203261","https://openalex.org/W149155564","https://openalex.org/W1586146176","https://openalex.org/W2410152555","https://openalex.org/W7098818815","https://openalex.org/W106626100","https://openalex.org/W2056172361"],"abstract_inverted_index":{"To":[0],"elucidate":[1],"the":[2,41,69,79,97,112,125,130,151,190,196,208],"regulatory":[3],"mechanisms":[4,236],"of":[5,43,71,81,96,101,124,132,143,154,161,189,210,213],"granulocyte-macrophage":[6],"colony-stimulating":[7],"factor":[8,29,51,59,63],"(GM-CSF)":[9],"production":[10,229],"in":[11,26,37,40,57,150,179,195,221],"human":[12,27],"myeloid":[13],"leukaemic":[14],"cells":[15,38,159,162,172],"we":[16],"studied":[17],"GM-CSF":[18,24,33,46,72,82,87,108,119,146,178,224,228],"gene":[19,88,120,225],"transcription,":[20],"mRNA":[21,83,109],"expression":[22],"and":[23,100,135,138,157,160,174,184,227,240],"secretion":[25,209],"growth":[28,50,58,98,191],"dependent":[30],"M-07e":[31,126,171,222],"cells.":[32,61],"transcript":[34],"was":[35,55,148],"detected":[36,149],"cultured":[39],"presence":[42],"interleukin-3":[44],"(IL-3).":[45],"or":[47],"mast":[48],"cell":[49],"(MGF),":[52],"whereas":[53],"it":[54],"undetectable":[56],"deprived":[60,94,163],"Growth":[62],"re-addition":[64],"induced,":[65],"within":[66],"2":[67,105],"h,":[68],"appearance":[70],"mRNA.":[73],"Nuclear":[74],"run-on":[75],"experiments":[76],"demonstrated":[77],"that":[78,170,200],"increase":[80],"levels":[84],"depends":[85],"on":[86],"transcription.":[89,121],"The":[90],"simultaneous":[91],"addition,":[92],"to":[93,141,181,206],"cells,":[95,223],"factor,":[99],"cycloheximide":[102],"(CHX)":[103],"for":[104,114,118,164],"h":[106],"inhibited":[107],"expression,":[110],"suggesting":[111],"requirement":[113],"newly":[115],"made":[116],"proteins":[117],"By":[122],"means":[123],"bioassay,":[127],"which":[128],"allows":[129],"detection":[131],"GM-CSF,":[133],"IL-3":[134,183,239],"MGF":[136],"activities,":[137],"neutralizing":[139],"antibodies":[140],"each":[142],"these":[144],"factors,":[145],"activity":[147,193],"cell-free":[152],"extract":[153],"both":[155,182],"IL-3-":[156],"MGF-sustained":[158],"24":[165],"h.":[166],"This":[167],"finding":[168],"demonstrates":[169],"produce":[173],"store":[175],"biologically":[176],"active":[177],"response":[180],"MGF.":[185,241],"In":[186],"contrast,":[187],"analysis":[188],"stimulatory":[192],"present":[194],"culture":[197],"supernatants":[198],"revealed":[199],"MGF,":[201],"unlike":[202,232],"IL-3,":[203],"is":[204],"able":[205],"induce":[207],"consistent":[211],"amounts":[212],"GM-CSF.":[214],"Taken":[215],"together,":[216],"our":[217],"results":[218],"suggest":[219],"that,":[220],"transcription":[226],"are":[230],"mediated,":[231],"its":[233],"secretion,":[234],"by":[235,238],"shared":[237]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}