{"id":"https://openalex.org/W1974921772","doi":"https://doi.org/10.1006/abio.2001.5548","title":"One-Step Unidirectional Cloning of Tandem Repeats of DNA Fragments: An Application for Fusion Protein Production","display_name":"One-Step Unidirectional Cloning of Tandem Repeats of DNA Fragments: An Application for Fusion Protein Production","publication_year":2002,"publication_date":"2002-04-01","ids":{"openalex":"https://openalex.org/W1974921772","doi":"https://doi.org/10.1006/abio.2001.5548","mag":"1974921772","pmid":"https://pubmed.ncbi.nlm.nih.gov/11906158"},"language":"en","primary_location":{"id":"doi:10.1006/abio.2001.5548","doi":"https://doi.org/10.1006/abio.2001.5548","is_oa":false,"landing_page_url":"https://doi.org/10.1006/abio.2001.5548","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028744476","display_name":"Zsolt Jobb\u00e1gy","orcid":"https://orcid.org/0000-0002-4143-4134","observed_orcids":["https://orcid.org/0000-0002-4143-4134"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zsolt Jobbagy","raw_affiliation_strings":["Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, MD 21205, USA","Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, MD 21205, USA","institution_ids":["https://openalex.org/I145311948"]},{"raw_affiliation_string":"Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205","institution_ids":["https://openalex.org/I145311948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020149913","display_name":"Jeffrey L. Ward","orcid":"https://orcid.org/0000-0002-0504-3259","observed_orcids":["https://orcid.org/0000-0002-0504-3259","https://orcid.org/0000-0003-3780-6780"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey L. Ward","raw_affiliation_strings":["Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205","institution_ids":["https://openalex.org/I145311948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111546423","display_name":"Shuy-Vang Toan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shuy-Vang Toan","raw_affiliation_strings":["Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205","institution_ids":["https://openalex.org/I145311948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002286218","display_name":"Gph Leung","orcid":"https://orcid.org/0000-0003-3529-9367","observed_orcids":["https://orcid.org/0000-0003-3529-9367"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George P.H. Leung","raw_affiliation_strings":["Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205","institution_ids":["https://openalex.org/I145311948"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021443559","display_name":"Chung\u2010Ming Tse","orcid":"https://orcid.org/0000-0002-1873-2029","observed_orcids":["https://orcid.org/0000-0002-1873-2029"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chung-Ming Tse","raw_affiliation_strings":["Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gastroenterology Division, Department of Medicine, Johns Hopkins University, 720 Rutland Avenue, Ross Research Building, Room 930, Baltimore, Maryland, 21205","institution_ids":["https://openalex.org/I145311948"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I145311948"],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":0.4339,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.55195515,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"303","issue":"1","first_page":"104","last_page":"107"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.7217000126838684,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.7217000126838684,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.12809999287128448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.033399999141693115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tandem-repeats","display_name":"tandem repeats","score":0.8180000185966492},{"id":"https://openalex.org/keywords/dna-cloning","display_name":"DNA cloning","score":0.7549999952316284},{"id":"https://openalex.org/keywords/fusion-protein","display_name":"fusion protein","score":0.7689999938011169},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7710000276565552},{"id":"https://openalex.org/keywords/dna-fragments","display_name":"DNA fragments","score":0.5289999842643738}],"concepts":[{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.7081670165061951},{"id":"https://openalex.org/C27149982","wikidata":"https://www.wikidata.org/wiki/Q3456514","display_name":"Tandem repeat","level":4,"score":0.6311606168746948},{"id":"https://openalex.org/C2777814067","wikidata":"https://www.wikidata.org/wiki/Q1752317","display_name":"Tandem","level":2,"score":0.5412842631340027},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5145193934440613},{"id":"https://openalex.org/C158525013","wikidata":"https://www.wikidata.org/wiki/Q2593739","display_name":"Fusion","level":2,"score":0.48044297099113464},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.46426889300346375},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.46112746000289917},{"id":"https://openalex.org/C2776235265","wikidata":"https://www.wikidata.org/wiki/Q18392052","display_name":"Fragment (logic)","level":2,"score":0.44180047512054443},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38050466775894165},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36134010553359985},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34374523162841797},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.21841707825660706},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.2158830463886261},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11338621377944946},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.09350290894508362},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.08342620730400085},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.07589367032051086},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.07236188650131226},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D020080","descriptor_name":"Tandem Repeat Sequences","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D026901","descriptor_name":"Membrane Transport Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D026901","descriptor_name":"Membrane Transport Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D033721","descriptor_name":"Equilibrative Nucleoside Transporter 1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1006/abio.2001.5548","doi":"https://doi.org/10.1006/abio.2001.5548","is_oa":false,"landing_page_url":"https://doi.org/10.1006/abio.2001.5548","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:11906158","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11906158","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:hub.hku.hk:10722/171280","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10722/171280","pdf_url":null,"source":{"id":"https://openalex.org/S4377196271","display_name":"The HKU Scholars Hub (University of Hong Kong)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I889458895","host_organization_name":"University of Hong Kong","host_organization_lineage":["https://openalex.org/I889458895"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1837910602","display_name":"NUCLEOSIDE TRANSPORT AND INTESTINAL CHLORIDE SECRETION","funder_award_id":"K08DK002737","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G6508418364","display_name":"NUCLEOSIDE TRANSPORTERS--STRUCTURE/FUNCTION AND REGULATI","funder_award_id":"R01CA085428","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W1594700207","https://openalex.org/W1973985383","https://openalex.org/W2012604733","https://openalex.org/W2018054062","https://openalex.org/W2068446924","https://openalex.org/W2070543174","https://openalex.org/W2130452592","https://openalex.org/W2185311629","https://openalex.org/W2419074890"],"related_works":["https://openalex.org/W1991761288","https://openalex.org/W2081957526","https://openalex.org/W2047472824","https://openalex.org/W25067752","https://openalex.org/W2012837813","https://openalex.org/W1992992125","https://openalex.org/W148511973","https://openalex.org/W89702538","https://openalex.org/W2012296409","https://openalex.org/W2040345742"],"abstract_inverted_index":null,"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}