{"id":"https://openalex.org/W1974479701","doi":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","title":"Correction: Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries","display_name":"Correction: Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries","publication_year":2013,"publication_date":"2013-12-03","ids":{"openalex":"https://openalex.org/W1974479701","doi":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","mag":"1974479701"},"language":"en","primary_location":{"id":"doi:10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","doi":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","is_oa":true,"landing_page_url":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","pdf_url":"https://journals.plos.org/plosone/article/file?type=printable&id=10.1371/journal.pone.0079120","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"volume":null,"type":"erratum","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?type=printable&id=10.1371/journal.pone.0079120","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014689360","display_name":"Thomas M. Blomquist","orcid":"https://orcid.org/0009-0003-4253-0691","observed_orcids":["https://orcid.org/0009-0003-4253-0691"]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Thomas M. Blomquist","raw_affiliation_strings":["Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013002142","display_name":"Erin L. Crawford","orcid":"https://orcid.org/0000-0002-4447-5863","observed_orcids":["https://orcid.org/0000-0002-4447-5863"]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Erin L. Crawford","raw_affiliation_strings":["Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008655325","display_name":"Jennie L. Lovett","orcid":"https://orcid.org/0000-0002-3710-9400","observed_orcids":["https://orcid.org/0000-0002-3710-9400"]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennie L. Lovett","raw_affiliation_strings":["Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110850649","display_name":"Ji\u2010Youn Yeo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jiyoun Yeo","raw_affiliation_strings":["Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048517343","display_name":"Lauren M. Stanoszek","orcid":"https://orcid.org/0000-0001-9159-6011","observed_orcids":["https://orcid.org/0000-0001-9159-6011"]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lauren M. Stanoszek","raw_affiliation_strings":["Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087470378","display_name":"Albert M. Levin","orcid":"https://orcid.org/0000-0002-6993-1669","observed_orcids":["https://orcid.org/0000-0002-6993-1669"]},"institutions":[{"id":"https://openalex.org/I154057602","display_name":"Henry Ford Health","ror":"https://ror.org/02kwnkm68","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I154057602"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Albert Levin","raw_affiliation_strings":["Department of Public Health Sciences, Henry Ford Health System, Detroit, Michigan, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Public Health Sciences, Henry Ford Health System, Detroit, Michigan, United States of America,","institution_ids":["https://openalex.org/I154057602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100405668","display_name":"Jia Li","orcid":"https://orcid.org/0000-0001-8043-1609","observed_orcids":["https://orcid.org/0000-0001-8043-1609"]},"institutions":[{"id":"https://openalex.org/I154057602","display_name":"Henry Ford Health","ror":"https://ror.org/02kwnkm68","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I154057602"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jia Li","raw_affiliation_strings":["Department of Public Health Sciences, Henry Ford Health System, Detroit, Michigan, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Public Health Sciences, Henry Ford Health System, Detroit, Michigan, United States of America,","institution_ids":["https://openalex.org/I154057602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102680536","display_name":"Mei Lu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154057602","display_name":"Henry Ford Health","ror":"https://ror.org/02kwnkm68","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I154057602"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mei Lu","raw_affiliation_strings":["Department of Public Health Sciences, Henry Ford Health System, Detroit, Michigan, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Public Health Sciences, Henry Ford Health System, Detroit, Michigan, United States of America,","institution_ids":["https://openalex.org/I154057602"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102979537","display_name":"Leming M. Shi","orcid":"https://orcid.org/0000-0002-7143-2033","observed_orcids":["https://orcid.org/0000-0002-7143-2033"]},"institutions":[{"id":"https://openalex.org/I1304557061","display_name":"National Center for Toxicological Research","ror":"https://ror.org/05jmhh281","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1304557061","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Leming Shi","raw_affiliation_strings":["National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas, United States of America","institution_ids":["https://openalex.org/I1304557061","https://openalex.org/I1320320070"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058525749","display_name":"Kenneth L. Muldrew","orcid":"https://orcid.org/0000-0002-7796-2270","observed_orcids":["https://orcid.org/0000-0002-7796-2270"]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth Muldrew","raw_affiliation_strings":["Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103524503","display_name":"James C. Willey","orcid":"https://orcid.org/0000-0002-1192-6824","observed_orcids":["https://orcid.org/0000-0002-1192-6824"]},"institutions":[{"id":"https://openalex.org/I90871651","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19","country_code":"US","type":"education","lineage":["https://openalex.org/I90871651"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James C. Willey","raw_affiliation_strings":["Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Toledo Health Sciences Campus, Toledo, Ohio, United States of America,","institution_ids":["https://openalex.org/I90871651"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5014689360"],"corresponding_institution_ids":["https://openalex.org/I90871651"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":null,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"8","issue":"12","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.861299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.861299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.062300000339746475,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.028200000524520874,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/targeted-rna-sequencing","display_name":"targeted RNA sequencing","score":0.8289999961853027},{"id":"https://openalex.org/keywords/transcript-quantification","display_name":"transcript quantification","score":0.8679999709129333},{"id":"https://openalex.org/keywords/pcr-bias","display_name":"PCR bias","score":0.6660000085830688},{"id":"https://openalex.org/keywords/molecular-diagnosis","display_name":"molecular diagnosis","score":0.7319999933242798},{"id":"https://openalex.org/keywords/biomarkers","display_name":"biomarkers","score":0.6470000147819519}],"concepts":[{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.7341036796569824},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5549114346504211},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.5532492995262146},{"id":"https://openalex.org/C2993206177","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon sequencing","level":4,"score":0.501793622970581},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.49330082535743713},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.46910223364830017},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43678438663482666},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3155312240123749},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.2778712511062622},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1348954737186432},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","doi":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","is_oa":true,"landing_page_url":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","pdf_url":"https://journals.plos.org/plosone/article/file?type=printable&id=10.1371/journal.pone.0079120","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2841899","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/articles/PMC3855815","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3855815","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3855815","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:76680fb96e674410ab8986dadb053b56","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/76680fb96e674410ab8986dadb053b56","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 8, Iss 12 (2013)","raw_type":"article"},{"id":"pmh:oai:doaj.org/article:df371d1c93e44f5a8d814b88f926f1ec","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/df371d1c93e44f5a8d814b88f926f1ec","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 8, Iss 12 (2013)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","doi":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","is_oa":true,"landing_page_url":"https://doi.org/10.1371/annotation/f77a351e-f57b-4102-b80c-6c4507beaba6","pdf_url":"https://journals.plos.org/plosone/article/file?type=printable&id=10.1371/journal.pone.0079120","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.428600013256073,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3768669729","display_name":null,"funder_award_id":"RC2-CA147652","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G3997483337","display_name":"Inherited genetic risk factors common to COPD and lung cancer.","funder_award_id":"R01HL108016","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G8379098902","display_name":null,"funder_award_id":"IMAT R21-CA138397","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320313633","display_name":"University of Toledo","ror":"https://ror.org/01pbdzh19"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1974479701.pdf","grobid_xml":"https://content.openalex.org/works/W1974479701.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W861134701","https://openalex.org/W1602584334","https://openalex.org/W1618865275","https://openalex.org/W1968035783","https://openalex.org/W1969369730","https://openalex.org/W1981509058","https://openalex.org/W2001526641","https://openalex.org/W2003169355","https://openalex.org/W2004869583","https://openalex.org/W2008840949","https://openalex.org/W2009458183","https://openalex.org/W2019331497","https://openalex.org/W2034355012","https://openalex.org/W2037113830","https://openalex.org/W2075044339","https://openalex.org/W2093009120","https://openalex.org/W2104145784","https://openalex.org/W2107727025","https://openalex.org/W2109243615","https://openalex.org/W2114859967","https://openalex.org/W2121211805","https://openalex.org/W2122878782","https://openalex.org/W2133980123","https://openalex.org/W2136746865","https://openalex.org/W2137064107","https://openalex.org/W2141599418","https://openalex.org/W2142922493","https://openalex.org/W2156631105","https://openalex.org/W2161106528","https://openalex.org/W2162098634","https://openalex.org/W4245576856","https://openalex.org/W4246020463"],"related_works":["https://openalex.org/W2060226894","https://openalex.org/W861134701","https://openalex.org/W2497129894","https://openalex.org/W2202473697","https://openalex.org/W2161673649","https://openalex.org/W2887643354","https://openalex.org/W2741009174","https://openalex.org/W2019955264","https://openalex.org/W2081752899","https://openalex.org/W133715763"],"abstract_inverted_index":{"Whole":[0],"transcriptome":[1,225],"RNA-sequencing":[2,25,226,301],"is":[3,8,28],"a":[4,58,95,168,254],"powerful":[5],"tool,":[6],"but":[7],"costly":[9],"and":[10,30,75,140,150,198,212,223,289,296],"yields":[11],"complex":[12],"data":[13],"sets":[14],"that":[15,27,67,116,274],"limit":[16],"its":[17],"utility":[18,107],"in":[19,81,303],"molecular":[20,304],"diagnostic":[21,52,305],"testing.A":[22],"targeted":[23,250,299],"quantitative":[24,300],"method":[26,66,281],"reproducible":[29,141],"reduces":[31],"the":[32,42,71,106,152,275,285],"number":[33,97],"of":[34,45,73,98,108,143,164,171,298],"sequencing":[35,63,159,241,270,291],"reads":[36,160,242,292],"required":[37,161,243],"to":[38,51,94,123,183,244,267],"measure":[39],"transcripts":[40,251],"over":[41,253],"full":[43],"range":[44,170,258],"expression":[46],"would":[47,117,137],"be":[48],"better":[49],"suited":[50],"testing.Toward":[53],"this":[54,109,135,239],"goal,":[55],"we":[56,111],"developed":[57],"competitive":[59,100,276],"multiplex":[60],"PCR-based":[61],"amplicon":[62,278],"library":[64,85,130,148,229,279],"preparation":[65,86,230,280],"a)":[68],"targets":[69,166],"only":[70],"sequences":[72],"interest":[74],"b)":[76],"controls":[77],"for":[78,162,294],"inter-target":[79],"variation":[80],"PCR":[82,114],"amplification":[83,120],"during":[84,129],"by":[87],"measuring":[88],"each":[89,144],"transcript":[90,119,145,165],"native":[91],"template":[92,101],"relative":[93],"known":[96],"synthetic":[99],"internal":[102],"standard":[103],"copies.To":[104],"determine":[105],"method,":[110,240],"intentionally":[112],"selected":[113],"conditions":[115],"cause":[118],"products":[121],"(amplicons)":[122],"converge":[124],"toward":[125],"equimolar":[126],"concentrations":[127],"(normalization)":[128],"preparation.We":[131],"then":[132],"tested":[133],"whether":[134],"approach":[136],"enable":[138],"accurate":[139],"quantification":[142,163],"across":[146,167],"multiple":[147],"preparations,":[149],"at":[151],"same":[153],"time":[154],"reduce":[155],"(through":[156],"normalization)":[157],"total":[158],"large":[169],"expression.We":[172],"demonstrate":[173,273],"excellent":[174],"reproducibility":[175],"(R":[176,201,219,235],"2":[177,202,220,236],"=":[178,203,221,237],"0.997)":[179],"with":[180,215],"97%":[181],"accuracy":[182],"detect":[184],"2-fold":[185],"change":[186],"using":[187,231],"External":[188],"RNA":[189],"Controls":[190],"Consortium":[191],"(ERCC)":[192],"reference":[193,210],"materials;":[194,211],"high":[195],"inter-day,":[196],"inter-site":[197],"inter-library":[199],"concordance":[200,214],"0.97-0.99)using":[204],"FDA":[205],"Sequencing":[206],"Quality":[207],"Control":[208],"(SEQC)":[209],"cross-platform":[213],"both":[216],"TaqMan":[217],"qPCR":[218],"0.96)":[222],"whole":[224],"following":[227],"''traditional''":[228],"Illumina":[232],"NGS":[233],"kits":[234],"0.94).Using":[238],"accurately":[245],"quantify":[246],"more":[247,261],"than":[248,262],"100":[249],"expressed":[252],"10":[255],"7":[256],"-fold":[257],"was":[259],"reduced":[260,290],"10,000-fold,":[263],"from":[264],"2.3610":[265],"9":[266],"1.4610":[268],"5":[269],"reads.These":[271],"studies":[272],"multiplex-PCR":[277],"presented":[282],"here":[283],"provides":[284],"quality":[286],"control,":[287],"reproducibility,":[288],"necessary":[293],"development":[295],"implementation":[297],"biomarkers":[302],"testing.":[306]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}