{"id":"https://openalex.org/W1974053931","doi":"https://doi.org/10.1016/0005-2744(74)90126-0","title":"Separation of Isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue","display_name":"Separation of Isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue","publication_year":1974,"publication_date":"1974-09-01","ids":{"openalex":"https://openalex.org/W1974053931","doi":"https://doi.org/10.1016/0005-2744(74)90126-0","mag":"1974053931","pmid":"https://pubmed.ncbi.nlm.nih.gov/4373066"},"language":"en","primary_location":{"id":"doi:10.1016/0005-2744(74)90126-0","doi":"https://doi.org/10.1016/0005-2744(74)90126-0","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0005-2744(74)90126-0","pdf_url":null,"source":{"id":"https://openalex.org/S4394906700","display_name":"Biochimica et Biophysica Acta (BBA) - Enzymology","issn_l":"0924-1086","issn":["0924-1086","0005-2744"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimica et Biophysica Acta (BBA) - Enzymology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005350108","display_name":"Lars I. Andersson","orcid":"https://orcid.org/0000-0002-5257-4052","observed_orcids":["https://orcid.org/0000-0002-5257-4052"]},"institutions":[{"id":"https://openalex.org/I187531555","display_name":"Lund University","ror":"https://ror.org/012a77v79","country_code":"SE","type":"education","lineage":["https://openalex.org/I187531555"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Lars Andersson","raw_affiliation_strings":["Biochemical Division, Chemical Centre, University of Lund, P.O. Box 740, S\u2010220 07 Lund 7, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemical Division, Chemical Centre, University of Lund, P.O. Box 740, S\u2010220 07 Lund 7, Sweden","institution_ids":["https://openalex.org/I187531555"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113570579","display_name":"Hans J\u00f6rnvall","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Hans J\u00f6rnvall","raw_affiliation_strings":["Department of Chemistry I, Karolinska Institute, S-10401, Stockholm 60 Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry I, Karolinska Institute, S-10401, Stockholm 60 Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113574174","display_name":"\u00c5ke \u00c5keson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"\u00c5ke \u00c5keson","raw_affiliation_strings":["Laboratory for Enzyme Research, Nobel Institute of Biochemistry, Karolinska Institute, S-10401, Stockholm 60 Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory for Enzyme Research, Nobel Institute of Biochemistry, Karolinska Institute, S-10401, Stockholm 60 Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5007955463","display_name":"Klaus Mosbach","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I187531555","display_name":"Lund University","ror":"https://ror.org/012a77v79","country_code":"SE","type":"education","lineage":["https://openalex.org/I187531555"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Klaus Mosbach","raw_affiliation_strings":["Biochemical Division, Chemical Centre, University of Lund, P.O. Box 740, S\u2010220 07 Lund 7, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemical Division, Chemical Centre, University of Lund, P.O. Box 740, S\u2010220 07 Lund 7, Sweden","institution_ids":["https://openalex.org/I187531555"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.9423,"has_fulltext":false,"cited_by_count":47,"citation_normalized_percentile":{"value":0.84562403,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"364","issue":"1","first_page":"1","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.490200012922287,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.490200012922287,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.1941000074148178,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11207","display_name":"Alcohol Consumption and Health Effects","score":0.0763000026345253,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/horse-liver-alcohol-dehydrogenase","display_name":"horse liver alcohol dehydrogenase","score":0.9509999752044678},{"id":"https://openalex.org/keywords/isozyme-separation","display_name":"isozyme separation","score":0.8700000047683716},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.8100000023841858},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.7570000290870667},{"id":"https://openalex.org/keywords/pyrazoles","display_name":"pyrazole derivatives","score":0.7919999957084656},{"id":"https://openalex.org/keywords/cholic-acid","display_name":"cholic acid","score":0.7630000114440918},{"id":"https://openalex.org/keywords/n-hexylamine","display_name":"n-hexylamine","score":0.7250000238418579}],"concepts":[{"id":"https://openalex.org/C2777344606","wikidata":"https://www.wikidata.org/wiki/Q410754","display_name":"Alcohol dehydrogenase","level":3,"score":0.7901512384414673},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.7407553195953369},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7387956976890564},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7027062177658081},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.6374198794364929},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.5413581728935242},{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.5108456015586853},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.4909808933734894},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4701274335384369},{"id":"https://openalex.org/C2775900799","wikidata":"https://www.wikidata.org/wiki/Q287415","display_name":"Cholic acid","level":3,"score":0.4307255446910858},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.41271620988845825},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37645387649536133},{"id":"https://openalex.org/C2779399885","wikidata":"https://www.wikidata.org/wiki/Q413288","display_name":"Bile acid","level":2,"score":0.13960209488868713}],"mesh":[{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D000362","descriptor_name":"Agar","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000429","descriptor_name":"Alcohol Oxidoreductases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000429","descriptor_name":"Alcohol Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006736","descriptor_name":"Horses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011720","descriptor_name":"Pyrazoles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012685","descriptor_name":"Sepharose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013056","descriptor_name":"Spectrophotometry, Ultraviolet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0005-2744(74)90126-0","doi":"https://doi.org/10.1016/0005-2744(74)90126-0","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0005-2744(74)90126-0","pdf_url":null,"source":{"id":"https://openalex.org/S4394906700","display_name":"Biochimica et Biophysica Acta (BBA) - Enzymology","issn_l":"0924-1086","issn":["0924-1086","0005-2744"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimica et Biophysica Acta (BBA) - Enzymology","raw_type":"journal-article"},{"id":"pmid:4373066","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4373066","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimica et biophysica acta","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W313659634","https://openalex.org/W1504516892","https://openalex.org/W1625660792","https://openalex.org/W1775749144","https://openalex.org/W1991474617","https://openalex.org/W2037644387","https://openalex.org/W2043392162","https://openalex.org/W2058382212","https://openalex.org/W2059689699","https://openalex.org/W2061357962","https://openalex.org/W2063873649","https://openalex.org/W2068095074","https://openalex.org/W2070404835","https://openalex.org/W2071016193","https://openalex.org/W2074118304","https://openalex.org/W2086576790","https://openalex.org/W2282054059","https://openalex.org/W2329174981","https://openalex.org/W4243191775"],"related_works":["https://openalex.org/W1975129398","https://openalex.org/W1140632153","https://openalex.org/W970059599","https://openalex.org/W2052490554","https://openalex.org/W2095289388","https://openalex.org/W2078417302","https://openalex.org/W1991145215","https://openalex.org/W1480651372","https://openalex.org/W1972816445","https://openalex.org/W63137966"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}