{"id":"https://openalex.org/W1973845730","doi":"https://doi.org/10.18388/abp.2000_4079","title":"Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis.","display_name":"Cloning, expression, and crystallization of Cpn60 proteins from Thermococcus litoralis.","publication_year":2000,"publication_date":"2000-03-31","ids":{"openalex":"https://openalex.org/W1973845730","doi":"https://doi.org/10.18388/abp.2000_4079","mag":"1973845730","pmid":"https://pubmed.ncbi.nlm.nih.gov/10961695"},"language":"en","primary_location":{"id":"doi:10.18388/abp.2000_4079","doi":"https://doi.org/10.18388/abp.2000_4079","is_oa":true,"landing_page_url":"https://doi.org/10.18388/abp.2000_4079","pdf_url":"https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4079/3137","source":{"id":"https://openalex.org/S166203837","display_name":"Acta Biochimica Polonica","issn_l":"0001-527X","issn":["0001-527X","1734-154X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800658","host_organization_name":"Polish Biochemical Society","host_organization_lineage":["https://openalex.org/P4320800658"],"host_organization_lineage_names":["Polish Biochemical Society"],"type":"journal"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Biochimica Polonica","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4079/3137","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085159378","display_name":"J. Osipiuk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1282105669","display_name":"Argonne National Laboratory","ror":"https://ror.org/05gvnxz63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1282105669","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Osipiuk","raw_affiliation_strings":["Argonne National Laboratory, Biosciences Division, IL 60439, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Argonne National Laboratory, Biosciences Division, IL 60439, USA","institution_ids":["https://openalex.org/I1282105669"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110943707","display_name":"M Sriram","orcid":"https://orcid.org/0000-0003-0997-5827","observed_orcids":["https://orcid.org/0000-0003-0997-5827"]},"institutions":[{"id":"https://openalex.org/I1282105669","display_name":"Argonne National Laboratory","ror":"https://ror.org/05gvnxz63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1282105669","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M Sriram","raw_affiliation_strings":["Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.;","institution_ids":["https://openalex.org/I1282105669"]}]},{"author_position":"middle","author":{"id":null,"display_name":"X Mai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"X Mai","raw_affiliation_strings":["Ja\u0153kowa Dolina 29 , 80-286 Gda\u00f1sk , Poland","University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ja\u0153kowa Dolina 29 , 80-286 Gda\u00f1sk , Poland","institution_ids":[]},{"raw_affiliation_string":"University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010547713","display_name":"Michael W. W. Adams","orcid":"https://orcid.org/0000-0002-9796-5014","observed_orcids":["https://orcid.org/0000-0002-9796-5014","https://orcid.org/0000-0003-0881-8870"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M W Adams","raw_affiliation_strings":["University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Georgia, Department of Biochemistry & Molecular Biology, Athens, GA 30602, U.S.A","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015893701","display_name":"A. Joachimiak","orcid":"https://orcid.org/0000-0003-2535-6209","observed_orcids":["https://orcid.org/0000-0003-2535-6209"]},"institutions":[{"id":"https://openalex.org/I1282105669","display_name":"Argonne National Laboratory","ror":"https://ror.org/05gvnxz63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1282105669","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Joachimiak","raw_affiliation_strings":["Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Argonne National Laboratory, Biosciences Division, 9700 S. Cass Ave., Argonne, IL 60439, U.S.A.;","institution_ids":["https://openalex.org/I1282105669"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1750,"currency":"USD","value_usd":1750},"apc_paid":{"value":1750,"currency":"USD","value_usd":1750},"fwci":0.0,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.09646913,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"47","issue":"1","first_page":"209","last_page":"214"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cpn60","display_name":"cpn60","score":0.8769999742507935},{"id":"https://openalex.org/keywords/chaperonin","display_name":"chaperonin","score":0.890999972820282},{"id":"https://openalex.org/keywords/thermococcus-litoralis","display_name":"Thermococcus litoralis","score":0.9769999980926514},{"id":"https://openalex.org/keywords/archaea","display_name":"archaea","score":0.746999979019165},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.7559999823570251},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.5690000057220459},{"id":"https://openalex.org/keywords/heterologous-expression-in-escherichia-coli","display_name":"heterologous expression in Escherichia coli","score":0.9129999876022339},{"id":"https://openalex.org/keywords/crystal-morphology","display_name":"crystal morphology","score":0.48399999737739563}],"concepts":[{"id":"https://openalex.org/C85755625","wikidata":"https://www.wikidata.org/wiki/Q24771589","display_name":"Chaperonin","level":3,"score":0.809616208076477},{"id":"https://openalex.org/C27911776","wikidata":"https://www.wikidata.org/wiki/Q834023","display_name":"Thermophile","level":3,"score":0.778113842010498},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.7169561386108398},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6908528804779053},{"id":"https://openalex.org/C2778899470","wikidata":"https://www.wikidata.org/wiki/Q2710532","display_name":"Thermococcus","level":4,"score":0.571839451789856},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.48638466000556946},{"id":"https://openalex.org/C550995028","wikidata":"https://www.wikidata.org/wiki/Q10872","display_name":"Archaea","level":3,"score":0.460679829120636},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44869449734687805},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3531554043292999},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.32394275069236755},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.22685253620147705},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018833","descriptor_name":"Chaperonins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018833","descriptor_name":"Chaperonins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018833","descriptor_name":"Chaperonins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018833","descriptor_name":"Chaperonins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018833","descriptor_name":"Chaperonins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018833","descriptor_name":"Chaperonins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019712","descriptor_name":"Thermococcus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019712","descriptor_name":"Thermococcus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019712","descriptor_name":"Thermococcus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019712","descriptor_name":"Thermococcus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019712","descriptor_name":"Thermococcus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019712","descriptor_name":"Thermococcus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.18388/abp.2000_4079","doi":"https://doi.org/10.18388/abp.2000_4079","is_oa":true,"landing_page_url":"https://doi.org/10.18388/abp.2000_4079","pdf_url":"https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4079/3137","source":{"id":"https://openalex.org/S166203837","display_name":"Acta Biochimica Polonica","issn_l":"0001-527X","issn":["0001-527X","1734-154X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800658","host_organization_name":"Polish Biochemical Society","host_organization_lineage":["https://openalex.org/P4320800658"],"host_organization_lineage_names":["Polish Biochemical Society"],"type":"journal"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Biochimica Polonica","raw_type":"journal-article"},{"id":"pmid:10961695","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10961695","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta biochimica Polonica","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.18388/abp.2000_4079","doi":"https://doi.org/10.18388/abp.2000_4079","is_oa":true,"landing_page_url":"https://doi.org/10.18388/abp.2000_4079","pdf_url":"https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4079/3137","source":{"id":"https://openalex.org/S166203837","display_name":"Acta Biochimica Polonica","issn_l":"0001-527X","issn":["0001-527X","1734-154X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800658","host_organization_name":"Polish Biochemical Society","host_organization_lineage":["https://openalex.org/P4320800658"],"host_organization_lineage_names":["Polish Biochemical Society"],"type":"journal"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Biochimica Polonica","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.8700000047683716,"display_name":"Life below water","id":"https://metadata.un.org/sdg/14"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2123841078","display_name":null,"funder_award_id":"W - 31 - 109 - Eng - 38","funder_id":"https://openalex.org/F4320306084","funder_display_name":"U.S. Department of Energy"}],"funders":[{"id":"https://openalex.org/F4320306084","display_name":"U.S. Department of Energy","ror":"https://ror.org/01bj3aw27"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1973845730.pdf","grobid_xml":"https://content.openalex.org/works/W1973845730.grobid-xml"},"referenced_works_count":3,"referenced_works":["https://openalex.org/W2031497022","https://openalex.org/W6629419795","https://openalex.org/W6674779418"],"related_works":["https://openalex.org/W7097322630","https://openalex.org/W2046269381","https://openalex.org/W391993344","https://openalex.org/W1875951678","https://openalex.org/W6983519790","https://openalex.org/W7110665053","https://openalex.org/W2026987207","https://openalex.org/W105552543","https://openalex.org/W2166749019","https://openalex.org/W2067590806"],"abstract_inverted_index":{"Two":[0],"genes":[1],"of":[2,25,40],"the":[3,26,32,57],"extreme":[4],"thermophilic":[5],"archaeon":[6],"Thermococcus":[7],"litoralis":[8],"homologous":[9],"to":[10,47,56],"those":[11],"that":[12],"code":[13],"for":[14],"Cpn60":[15,27,34],"chaperonins":[16],"were":[17],"cloned":[18],"and":[19,54],"expressed":[20],"in":[21,37],"Escherichia":[22],"coli.":[23],"Each":[24],"subunits":[28],"as":[29,31],"well":[30],"entire":[33],"complex":[35],"crystallize":[36],"a":[38,65],"variety":[39],"morphological":[41],"forms.":[42],"The":[43],"best":[44],"crystals":[45],"diffract":[46],"3.6":[48],"A":[49],"resolution":[50],"at":[51],"room":[52],"temperature":[53],"belong":[55],"space":[58],"group":[59],"1422":[60],"with":[61],"unit":[62],"cell":[63],"parameters":[64],"=":[66,68,72],"b":[67],"193.5":[69],"A,":[70],"c":[71],"204.2":[73],"A.":[74]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}