{"id":"https://openalex.org/W1973713086","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a130745","title":"Purification of Fibrinogen Using Cationic Detergent","display_name":"Purification of Fibrinogen Using Cationic Detergent","publication_year":1975,"publication_date":"1975-02-01","ids":{"openalex":"https://openalex.org/W1973713086","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a130745","mag":"1973713086","pmid":"https://pubmed.ncbi.nlm.nih.gov/805129"},"language":"en","primary_location":{"id":"doi:10.1093/oxfordjournals.jbchem.a130745","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a130745","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a130745","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003852678","display_name":"Susumu Kurioka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115498069","display_name":"Jikei University School of Medicine","ror":"https://ror.org/039ygjf22","country_code":"JP","type":"education","lineage":["https://openalex.org/I115498069"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Susumu KURIOKA","raw_affiliation_strings":["Department of Biochemistry, Jikei University School of Medicine Minato-ku, Tokyo 105"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Jikei University School of Medicine Minato-ku, Tokyo 105","institution_ids":["https://openalex.org/I115498069"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022835713","display_name":"Furnihide INOUE","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115498069","display_name":"Jikei University School of Medicine","ror":"https://ror.org/039ygjf22","country_code":"JP","type":"education","lineage":["https://openalex.org/I115498069"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Furnihide INOUE","raw_affiliation_strings":["Department of Biochemistry, Jikei University School of Medicine Minato-ku, Tokyo 105"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Jikei University School of Medicine Minato-ku, Tokyo 105","institution_ids":["https://openalex.org/I115498069"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081910282","display_name":"Fukuichi Nakada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32915989","display_name":"University of the Ryukyus","ror":"https://ror.org/02z1n9q24","country_code":"JP","type":"education","lineage":["https://openalex.org/I32915989"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Fukuichi NAKADA","raw_affiliation_strings":["Department of Biochemistry, College of Health Science, University of the Ryukyus Okinawa 902"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, College of Health Science, University of the Ryukyus Okinawa 902","institution_ids":["https://openalex.org/I32915989"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3276,"currency":"USD","value_usd":3276},"apc_paid":null,"fwci":0.3932,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.59997254,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"77","issue":"2","first_page":"457","last_page":"461"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fibrinogen-purification","display_name":"fibrinogen purification","score":0.9300000071525574},{"id":"https://openalex.org/keywords/cationic-detergents","display_name":"cationic detergents","score":0.8420000076293945},{"id":"https://openalex.org/keywords/protein-renaturation","display_name":"protein renaturation","score":0.9079999923706055},{"id":"https://openalex.org/keywords/human-plasma-proteins","display_name":"human plasma proteins","score":0.4490000009536743}],"concepts":[{"id":"https://openalex.org/C2779036427","wikidata":"https://www.wikidata.org/wiki/Q381899","display_name":"Fibrinogen","level":2,"score":0.9670804738998413},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7203699946403503},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6590308547019958},{"id":"https://openalex.org/C25642318","wikidata":"https://www.wikidata.org/wiki/Q1992532","display_name":"Incubation","level":2,"score":0.6373996138572693},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.49063315987586975},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.47580820322036743},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.422977089881897},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.11854055523872375},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000644","descriptor_name":"Quaternary Ammonium Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002210","descriptor_name":"Caprylates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003840","descriptor_name":"Deoxycholic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003902","descriptor_name":"Detergents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005779","descriptor_name":"Immunodiffusion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007122","descriptor_name":"Immunoelectrophoresis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012967","descriptor_name":"Sodium Dodecyl Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013229","descriptor_name":"Stearic Acids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1093/oxfordjournals.jbchem.a130745","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a130745","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a130745","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:805129","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/805129","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2162769196","https://openalex.org/W2080255436","https://openalex.org/W2086624966","https://openalex.org/W10712178","https://openalex.org/W2057222446","https://openalex.org/W2068065705","https://openalex.org/W2275456352","https://openalex.org/W2215117338","https://openalex.org/W4214524695","https://openalex.org/W2274636480"],"abstract_inverted_index":{"Stearyltrimethylammonium":[0],"chloride":[1],"was":[2,11,39,78],"used":[3],"to":[4,18,41],"isolate":[5],"human":[6],"fibrinogen,":[7],"and":[8,34,69,100],"purified":[9,52,76,88],"protein":[10],"obtained":[12],"by":[13],"removing":[14],"the":[15,49,75,87,106],"detergent":[16],"bound":[17],"it.":[19],"Medium":[20],"consisting":[21],"of":[22,46,74,97,113],"0.015--0.03":[23],"mM":[24],"fibrinogen-detergent":[25],"complex,":[26],"0.85":[27],"M":[28,31],"NaCl,":[29],"0.03":[30],"sodium":[32],"caprylate,":[33],"30":[35],"per":[36,81],"cent":[37],"ethanol":[38],"found":[40],"be":[42],"effective":[43],"for":[44,61],"renaturation":[45],"fibrinogen":[47,53,77,89,99,107],"from":[48],"complex.":[50],"The":[51,72],"did":[54],"not":[55],"form":[56],"any":[57],"fibrils":[58],"on":[59],"incubation":[60],"15":[62],"days":[63],"with":[64,94],"Ca-2+":[65],"at":[66],"pH":[67],"7.2,":[68],"37":[70],"degrees.":[71],"clottability":[73],"over":[79],"99":[80],"cent.":[82],"Immunochemical":[83],"studies":[84],"showed":[85],"that":[86],"produced":[90],"one":[91],"precipitation":[92],"line":[93],"a":[95,111],"mixture":[96],"anti-human":[98,101],"serum.":[102],"Although":[103],"highly":[104],"purified,":[105],"preparation":[108],"still":[109],"contained":[110],"trace":[112],"plasminogen.":[114]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}