{"id":"https://openalex.org/W1973425101","doi":"https://doi.org/10.1046/j.1365-313x.1996.10040745.x","title":"Fluorescent imaging of GUS activity and RT\u2010PCR analysis of gene expression in the shoot apical meristem","display_name":"Fluorescent imaging of GUS activity and RT\u2010PCR analysis of gene expression in the shoot apical meristem","publication_year":1996,"publication_date":"1996-10-01","ids":{"openalex":"https://openalex.org/W1973425101","doi":"https://doi.org/10.1046/j.1365-313x.1996.10040745.x","mag":"1973425101","pmid":"https://pubmed.ncbi.nlm.nih.gov/8893550"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-313x.1996.10040745.x","doi":"https://doi.org/10.1046/j.1365-313x.1996.10040745.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-313x.1996.10040745.x","pdf_url":null,"source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016012911","display_name":"Andrew J. Fleming","orcid":"https://orcid.org/0000-0002-9703-0745","observed_orcids":["https://orcid.org/0000-0002-9703-0745"]},"institutions":[{"id":"https://openalex.org/I118564535","display_name":"University of Bern","ror":"https://ror.org/02k7v4d05","country_code":"CH","type":"education","lineage":["https://openalex.org/I118564535"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Andrew J. Fleming","raw_affiliation_strings":["Institute of Plant Physiology, University of Berne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Physiology, University of Berne, Switzerland","institution_ids":["https://openalex.org/I118564535"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063563968","display_name":"Thianda Manzara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thianda Manzara","raw_affiliation_strings":["Department of Plant Biology, University of California, Berkeley, CA 94720, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Biology, University of California, Berkeley, CA 94720, USA","institution_ids":["https://openalex.org/I95457486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006955632","display_name":"Wilhelm Gruissem","orcid":"https://orcid.org/0000-0002-1872-2998","observed_orcids":["https://orcid.org/0000-0002-1872-2998"]},"institutions":[{"id":"https://openalex.org/I95457486","display_name":"University of California, Berkeley","ror":"https://ror.org/01an7q238","country_code":"US","type":"education","lineage":["https://openalex.org/I95457486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wilhelm Gruissem","raw_affiliation_strings":["Department of Plant Biology, University of California, Berkeley, CA 94720, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Biology, University of California, Berkeley, CA 94720, USA","institution_ids":["https://openalex.org/I95457486"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002465455","display_name":"C.J. Kuhlemeier","orcid":"https://orcid.org/0000-0003-2440-5937","observed_orcids":["https://orcid.org/0000-0003-2440-5937"]},"institutions":[{"id":"https://openalex.org/I118564535","display_name":"University of Bern","ror":"https://ror.org/02k7v4d05","country_code":"CH","type":"education","lineage":["https://openalex.org/I118564535"]}],"countries":["CH"],"is_corresponding":true,"raw_author_name":"Cris Kuhlemeier","raw_affiliation_strings":["Institute of Plant Physiology, University of Berne, Altenbergrain 21, CH-3013 Bern, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Physiology, University of Berne, Altenbergrain 21, CH-3013 Bern, Switzerland","institution_ids":["https://openalex.org/I118564535"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5002465455"],"corresponding_institution_ids":["https://openalex.org/I118564535"],"apc_list":{"value":5450,"currency":"USD","value_usd":5450},"apc_paid":null,"fwci":3.0943,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.90358908,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"10","issue":"4","first_page":"745","last_page":"754"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.9624000191688538,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.9624000191688538,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.012500000186264515,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11268","display_name":"Light effects on plants","score":0.00800000037997961,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/shoot-apical-meristem","display_name":"shoot apical meristem","score":0.9860000014305115},{"id":"https://openalex.org/keywords/gus-reporter-gene","display_name":"GUS reporter gene","score":0.6460000276565552},{"id":"https://openalex.org/keywords/fluorescence-imaging","display_name":"fluorescence imaging","score":0.7210000157356262},{"id":"https://openalex.org/keywords/rbcs-gene","display_name":"rbcS gene","score":0.781000018119812},{"id":"https://openalex.org/keywords/ribulose-bisphosphate-carboxylase-oxygenase","display_name":"ribulose bisphosphate carboxylase","score":0.871999979019165},{"id":"https://openalex.org/keywords/promoter-activity","display_name":"promoter activity","score":0.5699999928474426},{"id":"https://openalex.org/keywords/light-controlled-gene-expression","display_name":"light-regulated gene expression","score":0.7120000123977661},{"id":"https://openalex.org/keywords/photosynthetic-gene-expression","display_name":"photosynthetic gene expression","score":0.7770000100135803},{"id":"https://openalex.org/keywords/tomato","display_name":"tomato","score":0.8240000009536743},{"id":"https://openalex.org/keywords/gene-expression-patterns","display_name":"gene expression patterns","score":0.4339999854564667}],"concepts":[{"id":"https://openalex.org/C126021549","wikidata":"https://www.wikidata.org/wiki/Q207604","display_name":"Meristem","level":3,"score":0.9600726366043091},{"id":"https://openalex.org/C184750944","wikidata":"https://www.wikidata.org/wiki/Q557677","display_name":"Primordium","level":3,"score":0.8659520149230957},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8256372213363647},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.6413885951042175},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5955718159675598},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5686385035514832},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4923657178878784},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4415883421897888},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.43404191732406616},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.394202321767807},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3640769124031067},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.1635339856147766}],"mesh":[{"descriptor_ui":"D005810","descriptor_name":"Multigene Family","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D009024","descriptor_name":"Morphogenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012273","descriptor_name":"Ribulose-Bisphosphate Carboxylase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D012273","descriptor_name":"Ribulose-Bisphosphate Carboxylase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017343","descriptor_name":"Genes, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018506","descriptor_name":"Gene Expression Regulation, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D018519","descriptor_name":"Meristem","qualifier_ui":"Q000033","qualifier_name":"anatomy & histology","is_major_topic":false},{"descriptor_ui":"D018519","descriptor_name":"Meristem","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D018519","descriptor_name":"Meristem","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D018551","descriptor_name":"Solanum lycopersicum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1046/j.1365-313x.1996.10040745.x","doi":"https://doi.org/10.1046/j.1365-313x.1996.10040745.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-313x.1996.10040745.x","pdf_url":null,"source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},{"id":"pmid:8893550","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8893550","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant journal : for cell and molecular biology","raw_type":"Journal Article"},{"id":"pmh:doi:10.7892/boris.97860","doi":null,"is_oa":false,"landing_page_url":"https://boris-portal.unibe.ch/handle/20.500.12422/151318","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"pmh:oai:boris.unibe.ch:97860","doi":null,"is_oa":false,"landing_page_url":"https://boris.unibe.ch/97860/","pdf_url":null,"source":{"id":"https://openalex.org/S4306401086","display_name":"Bern Open Repository and Information System (University of Bern)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I118564535","host_organization_name":"University of Bern","host_organization_lineage":["https://openalex.org/I118564535"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Fleming, Andrew J; Manzara, Thiande; Gruissem, Wilhelm; Kuhlemeier, Cris (1996). Fluorescent imaging of GUS activity and RT-PCR analysis of gene expression in the shoot apical meristem. The Plant Journal, 10(4), pp. 745-754. Blackwell Science 10.1046/j.1365-313X.1996.10040745.x &lt;http://dx.doi.org/10.1046/j.1365-313X.1996.10040745.x&gt;","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2022440940","https://openalex.org/W1983070224","https://openalex.org/W2143108234","https://openalex.org/W2134377320","https://openalex.org/W2111109741","https://openalex.org/W6902643779","https://openalex.org/W1967097994","https://openalex.org/W2064066065","https://openalex.org/W2030493732","https://openalex.org/W2109761319"],"abstract_inverted_index":{"The":[0],"use":[1],"of":[2,16,27,46,58,69,81,90,159,168,180,193,225],"promoter-reporter":[3],"gene":[4,17,49,226],"constructs":[5],"in":[6,13,24,54,63,94,99,105,130,141,155,207,220,229],"transgenic":[7],"plants":[8],"is":[9,75],"a":[10,39,165],"powerful":[11],"tool":[12],"the":[14,25,32,44,55,88,100,106,110,142,160,169,178,181,191,198,217,221,230],"analysis":[15,45,68,192],"expression":[18,50,98,104,227],"which":[19,150,189,211],"can,":[20],"however,":[21],"be":[22,139,213],"limited":[23],"resolution":[26,53],"small":[28],"structures,":[29],"such":[30,194],"as":[31],"apical":[33,56,101,143,231],"meristem.":[34,232],"This":[35],"paper":[36],"reports":[37],"on":[38,174],"fluorescent":[40],"imaging":[41],"technique":[42,62],"for":[43,124],"GUS":[47],"reporter":[48],"to":[51,86,215],"cellular":[52,201],"meristem":[57,144],"tomato.":[59],"Using":[60],"this":[61],"combination":[64],"with":[65,122],"an":[66,147,206],"RT-PCR":[67],"RBS":[70],"gene-specific":[71],"transcript":[72,153],"levels,":[73],"it":[74],"shown":[76],"that:":[77],"5'":[78],"upstream":[79],"sequences":[80],"RBCS":[82,135,152],"genes":[83,111],"are":[84,116],"sufficient":[85],"mimic":[87],"pattern":[89],"transcripts":[91,123],"revealed":[92],"by":[93,145],"situ":[95],"hybridisation":[96],"(no":[97],"meristem,":[102],"high":[103],"initiated":[107],"leaf":[108,131,175],"primordia);":[109],"RBCS2,":[112],"RBCS3A":[113],"and":[114,126,133,177,200,203,223],"RBCS3B":[115],"transcriptionally":[117],"activated":[118],"upon":[119],"primordium":[120],"initiation":[121,176,222],"RBCS1":[125],"RBCS3C":[127],"accumulating":[128],"later":[129],"development;":[132],"that":[134],"promoter":[136],"activity":[137],"cannot":[138],"induced":[140],"light,":[146],"environmental":[148],"signal":[149],"elevates":[151],"level":[154],"other":[156],"aerial":[157],"parts":[158],"plant.":[161],"These":[162],"data":[163],"provide":[164],"detailed":[166],"picture":[167],"complex":[170,195],"transcriptional":[171],"events":[172,196],"occurring":[173],"establishment":[179],"photosynthetic":[182],"machinery;":[183],"they":[184,204],"describe":[185],"two":[186],"complementary":[187],"techniques":[188],"allow":[190],"at":[197],"tissue":[199],"level;":[202],"characterize":[205],"vivo":[208],"assay":[209],"system":[210],"can":[212],"used":[214],"analyse":[216],"factors":[218],"involved":[219],"maintenance":[224],"patterns":[228]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
