{"id":"https://openalex.org/W1972467658","doi":"https://doi.org/10.1177/108705710200700104","title":"Determination of Ligand-MurB Interactions by Isothermal Denaturation: Applicatio as a Secondary Assay to Complement High Throughput Screening","display_name":"Determination of Ligand-MurB Interactions by Isothermal Denaturation: Applicatio as a Secondary Assay to Complement High Throughput Screening","publication_year":2002,"publication_date":"2002-02-01","ids":{"openalex":"https://openalex.org/W1972467658","doi":"https://doi.org/10.1177/108705710200700104","mag":"1972467658","pmid":"https://pubmed.ncbi.nlm.nih.gov/11897052"},"language":"en","primary_location":{"id":"doi:10.1177/108705710200700104","doi":"https://doi.org/10.1177/108705710200700104","is_oa":true,"landing_page_url":"https://doi.org/10.1177/108705710200700104","pdf_url":null,"source":{"id":"https://openalex.org/S4387277810","display_name":"SLAS DISCOVERY","issn_l":"2472-5552","issn":["2472-5552","2472-5560"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Discovery","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1177/108705710200700104","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088542908","display_name":"Ronald W. Sarver","orcid":"https://orcid.org/0009-0005-9980-0247","observed_orcids":["https://orcid.org/0009-0005-9980-0247"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ronald W. Sarver","raw_affiliation_strings":["Structural and Medicinal Chemistry, Pharmacia Corporation, Kalamazoo, MI 49001, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Structural and Medicinal Chemistry, Pharmacia Corporation, Kalamazoo, MI 49001, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065887449","display_name":"Joseph M. Rogers","orcid":"https://orcid.org/0000-0002-1313-4089","observed_orcids":["https://orcid.org/0000-0002-1313-4089"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph M. Rogers","raw_affiliation_strings":["Structural and Medicinal Chemistry, Pharmacia Corporation, Kalamazoo, MI"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Structural and Medicinal Chemistry, Pharmacia Corporation, Kalamazoo, MI","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111820087","display_name":"Dennis E. Van Epps","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dennis E. Epps","raw_affiliation_strings":["Discovery Technologies, Pharmacia Corp., Kalamazoo, MI"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Discovery Technologies, Pharmacia Corp., Kalamazoo, MI","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":2070,"currency":"USD","value_usd":2070},"apc_paid":{"value":2070,"currency":"USD","value_usd":2070},"fwci":0.3873,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.46974996,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"7","issue":"1","first_page":"21","last_page":"28"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.7211999893188477,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.7211999893188477,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.17759999632835388,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.06260000169277191,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/murb","display_name":"MurB","score":0.9769999980926514},{"id":"https://openalex.org/keywords/enzyme-inhibitors","display_name":"enzyme inhibitors","score":0.5709999799728394},{"id":"https://openalex.org/keywords/enzyme-ligand-interactions","display_name":"enzyme-ligand interactions","score":0.8180000185966492},{"id":"https://openalex.org/keywords/flavin-cofactor","display_name":"flavin cofactor","score":0.7680000066757202},{"id":"https://openalex.org/keywords/inhibitor-specificity","display_name":"inhibitor specificity","score":0.7080000042915344},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.6470000147819519},{"id":"https://openalex.org/keywords/fluorescence-quenching","display_name":"fluorescence quenching","score":0.6769999861717224},{"id":"https://openalex.org/keywords/circular-dichroism","display_name":"circular dichroism","score":0.7279999852180481},{"id":"https://openalex.org/keywords/differential-scanning-calorimetry","display_name":"differential scanning calorimetry","score":0.8709999918937683}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8040571212768555},{"id":"https://openalex.org/C2776923230","wikidata":"https://www.wikidata.org/wiki/Q16955798","display_name":"Denaturation (fissile materials)","level":2,"score":0.763934850692749},{"id":"https://openalex.org/C156911925","wikidata":"https://www.wikidata.org/wiki/Q643384","display_name":"Isothermal titration calorimetry","level":2,"score":0.527645468711853},{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.5190752148628235},{"id":"https://openalex.org/C33093398","wikidata":"https://www.wikidata.org/wiki/Q174337","display_name":"Flavin group","level":3,"score":0.5148574709892273},{"id":"https://openalex.org/C121745418","wikidata":"https://www.wikidata.org/wiki/Q585536","display_name":"Quenching (fluorescence)","level":3,"score":0.4769948124885559},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.46863770484924316},{"id":"https://openalex.org/C21463570","wikidata":"https://www.wikidata.org/wiki/Q3025038","display_name":"Hyperchromicity","level":3,"score":0.4226498603820801},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.4155391454696655},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3161088228225708},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.2840825021266937},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24467310309410095},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.07808306813240051},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001331","descriptor_name":"Automation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001671","descriptor_name":"Biochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002152","descriptor_name":"Calorimetry, Differential Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002237","descriptor_name":"Carbohydrate Dehydrogenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002237","descriptor_name":"Carbohydrate Dehydrogenases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014466","descriptor_name":"Ultraviolet Rays","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1177/108705710200700104","doi":"https://doi.org/10.1177/108705710200700104","is_oa":true,"landing_page_url":"https://doi.org/10.1177/108705710200700104","pdf_url":null,"source":{"id":"https://openalex.org/S4387277810","display_name":"SLAS DISCOVERY","issn_l":"2472-5552","issn":["2472-5552","2472-5560"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Discovery","raw_type":"journal-article"},{"id":"pmid:11897052","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11897052","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biomolecular screening","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1177/108705710200700104","doi":"https://doi.org/10.1177/108705710200700104","is_oa":true,"landing_page_url":"https://doi.org/10.1177/108705710200700104","pdf_url":null,"source":{"id":"https://openalex.org/S4387277810","display_name":"SLAS DISCOVERY","issn_l":"2472-5552","issn":["2472-5552","2472-5560"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-40"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Discovery","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W90753591","https://openalex.org/W1484534097","https://openalex.org/W1983344500","https://openalex.org/W1988331437","https://openalex.org/W1991697099","https://openalex.org/W2040952692","https://openalex.org/W2045145741","https://openalex.org/W2087225699"],"related_works":["https://openalex.org/W4244138513","https://openalex.org/W2045145741","https://openalex.org/W2099000443","https://openalex.org/W2006973448","https://openalex.org/W2158768415","https://openalex.org/W1985034086","https://openalex.org/W1998168399","https://openalex.org/W1966373074","https://openalex.org/W2961628186","https://openalex.org/W4386438443"],"abstract_inverted_index":{"We":[0,90,159],"used":[1,46,80],"a":[2,56,172,184],"temperature-jump":[3],"isothermal":[4,169],"denaturation":[5,66,98,137,170],"procedure":[6],"with":[7,125,168],"various":[8,165],"methods":[9,27,167],"of":[10,16,19,28,31,34,51,64,70,76,86,99,105,122,135,144,163],"detection":[11,166],"to":[12,47,81,118,175],"evaluate":[13],"the":[14,49,60,65,71,83,87,93,97,102,108,113,120,123,126,130,136,142,157,164],"quality":[15],"putative":[17],"inhibitors":[18,116],"MurB":[20,100],"discovered":[21],"by":[22,74],"high-throughput":[23],"screening.":[24],"Three":[25],"optical":[26],"detection-ultraviolet":[29],"hyperchromicity":[30],"absorbance,":[32],"fluorescence":[33,78,146],"bound":[35],"dyes,":[36],"and":[37,55,68,111],"circular":[38],"dichroism-as":[39],"well":[40,140],"as":[41,139,141],"differential":[42],"scanning":[43],"calorimetry":[44],"were":[45,79],"dissect":[48],"effects":[50],"two":[52,114],"chemical":[53,115],"compounds":[54,72,152],"natural":[57],"substrate":[58],"on":[59,147],"enzyme.":[61,158],"The":[62,133],"kinetics":[63,134],"process":[67,138],"binding":[69,148],"detected":[73],"quenching":[75],"flavin":[77,106,145],"quantitate":[82],"dose":[84],"dependencies":[85],"ligand":[88],"effects.":[89],"found":[91],"that":[92,112,150,161],"first":[94],"step":[95],"in":[96],"is":[101],"rapid":[103],"loss":[104,143],"from":[107],"active":[109],"site":[110],"appeared":[117],"destabilize":[119],"interaction":[121],"cofactor":[124],"enzyme":[127],"but":[128],"stabilize":[129],"global":[131],"unfolding.":[132],"established":[149],"both":[151],"had":[153],"nanomolar":[154],"affinities":[155],"for":[156,179,183],"showed":[160],"coupling":[162],"yields":[171],"powerful":[173],"regimen":[174],"provide":[176],"analytical":[177],"data":[178],"assessing":[180],"inhibitor":[181],"specificity":[182],"protein":[185],"target.":[186]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}
