{"id":"https://openalex.org/W1972012253","doi":"https://doi.org/10.1073/pnas.75.6.2839","title":"An ordered sequence of events is required before BALB/c-3T3 cells become committed to DNA synthesis","display_name":"An ordered sequence of events is required before BALB/c-3T3 cells become committed to DNA synthesis","publication_year":1978,"publication_date":"1978-06-01","ids":{"openalex":"https://openalex.org/W1972012253","doi":"https://doi.org/10.1073/pnas.75.6.2839","mag":"1972012253","pmid":"https://pubmed.ncbi.nlm.nih.gov/275855","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/392660"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.75.6.2839","doi":"https://doi.org/10.1073/pnas.75.6.2839","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.75.6.2839","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W1972012253"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC392660/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113725812","display_name":"Warren Jack Pledger","orcid":"https://orcid.org/0000-0001-5306-7506","observed_orcids":["https://orcid.org/0000-0001-5306-7506"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W. J. Pledger","raw_affiliation_strings":["Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115","Center for Blood Research and Department of Nutrition, Harvard University School of Public Health, Boston, Massachusetts 02115","Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115; Laboratory of Tumor Biology,","Sidney Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Center for Blood Research and Department of Nutrition, Harvard University School of Public Health, Boston, Massachusetts 02115","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115; Laboratory of Tumor Biology,","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Sidney Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115;","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070312457","display_name":"Charles D. Stiles","orcid":"https://orcid.org/0000-0003-1677-1195","observed_orcids":["https://orcid.org/0000-0003-1677-1195"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C. D. Stiles","raw_affiliation_strings":["Laboratory of Tumor Biology, Sidney Farber Cancer Institute, Boston, Massachusetts 02115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Tumor Biology, Sidney Farber Cancer Institute, Boston, Massachusetts 02115","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111784805","display_name":"Harry N. Antoniades","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H. N. Antoniades","raw_affiliation_strings":["Center for Blood Research, Boston, Massachusetts 02115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Blood Research, Boston, Massachusetts 02115","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111948487","display_name":"Charles D. Scher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. D. Scher","raw_affiliation_strings":["Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115","Center for Blood Research and Department of Nutrition, Harvard University School of Public Health, Boston, Massachusetts 02115","Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115; Laboratory of Tumor Biology,","Sidney Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Center for Blood Research and Department of Nutrition, Harvard University School of Public Health, Boston, Massachusetts 02115","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Pediatrics, Sidney Farber Cancer Institute and the Childrens' Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02115; Laboratory of Tumor Biology,","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Sidney Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115;","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":18.8481,"has_fulltext":false,"cited_by_count":451,"citation_normalized_percentile":{"value":0.99553923,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"75","issue":"6","first_page":"2839","last_page":"2843"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.6054999828338623,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.6054999828338623,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.20559999346733093,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.06499999761581421,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/platelet-derived-growth-factor","display_name":"platelet-derived growth factor","score":0.8690000176429749},{"id":"https://openalex.org/keywords/platelet-poor-plasma","display_name":"platelet-poor plasma","score":0.8320000171661377},{"id":"https://openalex.org/keywords/balb-c-3t3-cells","display_name":"BALB/c 3T3 cells","score":0.7760000228881836},{"id":"https://openalex.org/keywords/g0-g1-arrest","display_name":"G0/G1 arrest","score":0.6930000185966492},{"id":"https://openalex.org/keywords/s-phase-entry","display_name":"S phase entry","score":0.8510000109672546},{"id":"https://openalex.org/keywords/cell-cycle-progression","display_name":"cell cycle progression","score":0.6499999761581421},{"id":"https://openalex.org/keywords/growth-arrest","display_name":"growth arrest","score":0.578000009059906},{"id":"https://openalex.org/keywords/cycloheximide","display_name":"cycloheximide","score":0.8370000123977661},{"id":"https://openalex.org/keywords/hydroxyurea","display_name":"hydroxyurea","score":0.9769999980926514},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.3709999918937683}],"concepts":[{"id":"https://openalex.org/C64241487","wikidata":"https://www.wikidata.org/wiki/Q5205774","display_name":"DNA synthesis","level":3,"score":0.772551953792572},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5057921409606934},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.504440188407898},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.48812994360923767},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43856891989707947},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33399099111557007},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25570905208587646},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.13328656554222107},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001792","descriptor_name":"Blood Platelets","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D001798","descriptor_name":"Blood Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002453","descriptor_name":"Cell Cycle","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003260","descriptor_name":"Contact Inhibition","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D003513","descriptor_name":"Cycloheximide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D006133","descriptor_name":"Growth Substances","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D006918","descriptor_name":"Hydroxyurea","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1073/pnas.75.6.2839","doi":"https://doi.org/10.1073/pnas.75.6.2839","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.75.6.2839","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmid:275855","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/275855","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:392660","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC392660/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmh:oai:mouseion.jax.org:ssbb1978-4237","doi":null,"is_oa":false,"landing_page_url":"https://mouseion.jax.org/ssbb1978/3238","pdf_url":null,"source":{"id":"https://openalex.org/S4377196421","display_name":"The Mouseion at the JAXlibrary (Jackson Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210115256","host_organization_name":"Jackson Laboratory","host_organization_lineage":["https://openalex.org/I4210115256"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Subject Strain Bibliography 1978","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:392660","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC392660/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[{"score":0.46810001134872437,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306095","display_name":"American Cancer Society","ror":"https://ror.org/02e463172"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1971182101","https://openalex.org/W1991216151","https://openalex.org/W1992538884","https://openalex.org/W2000054385","https://openalex.org/W2006954794","https://openalex.org/W2009849541","https://openalex.org/W2034604567","https://openalex.org/W2052096039","https://openalex.org/W2052970532","https://openalex.org/W2053632632","https://openalex.org/W2064670731","https://openalex.org/W2067072430","https://openalex.org/W2094891357","https://openalex.org/W2106386686","https://openalex.org/W2109517079","https://openalex.org/W2113139348","https://openalex.org/W2156905839","https://openalex.org/W2328817220"],"related_works":["https://openalex.org/W1992538884","https://openalex.org/W2070631215","https://openalex.org/W2096670974","https://openalex.org/W2126682488","https://openalex.org/W2021034854","https://openalex.org/W2028502494","https://openalex.org/W2054568885","https://openalex.org/W2111928271","https://openalex.org/W2039281296","https://openalex.org/W2050049952"],"abstract_inverted_index":{"An":[0],"ordered":[1],"sequence":[2,96],"of":[3,27,59,68,83,110,116,129,153,189,194,255,269,293,307],"events":[4],"must":[5],"be":[6],"completed":[7],"before":[8,65,209,227],"cells":[9,33,44,62,103,133,161,195,246,297],"become":[10,35],"committed":[11,249],"to":[12,34,37,45,106,122,134,157,250,281,298],"synthesize":[13],"DNA.":[14],"A":[15],"platelet-derived":[16],"growth":[17],"factor":[18],"(PDGF),":[19],"present":[20],"in":[21,92,99,145,304],"heated":[22],"(100":[23],"degrees":[24],")":[25],"extracts":[26],"human":[28],"platelets,":[29],"induces":[30,41],"density-inhibited":[31],"BALB/c-3T3":[32],"competent":[36,43,102,160],"proliferate.":[38],"Platelet-poor":[39],"plasma":[40,111,130,166,198,217,256,275],"these":[42,244],"leave":[46,176],"the":[47,55,66,73,81,86,93,126,132,136,151,154,171,177,187,261,274,294,300,305],"competence":[48,178],"point,":[49],"progress":[50],"through":[51],"G(0)/G(1),":[52],"and":[53,118,243],"enter":[54,135,299],"S":[56,87,137,262,301],"phase.":[57,88,138],"Treatment":[58],"G(0)-arrested,":[60],"incompetent":[61],"with":[63,165,197,216,264],"plasma,":[64],"addition":[67,254],"PDGF,":[69],"did":[70,174],"not":[71,175,248,289],"shorten":[72],"latent":[74],"period":[75,141],"for":[76,113,167,199,218,237],"DNA":[77,143,182,210,228,251,282],"synthesis":[78,183,283],"or":[79],"increase":[80],"rate":[82,268],"entry":[84,259,270],"into":[85,260],"Growth":[89],"arrest":[90],"points":[91],"plasma-dependent":[94,278],"progression":[95],"were":[97,104,119,163,247],"detected":[98],"G(0)/G(1).":[100],"PDGF-treated":[101,159],"exposed":[105],"an":[107,265],"optimal":[108],"concentration":[109],"(5%)":[112],"various":[114],"lengths":[115],"time":[117],"then":[120],"transferred":[121],"medium":[123],"lacking":[124],"plasma;":[125],"subsequent":[127],"readdition":[128,188],"stimulated":[131],"The":[139,253],"lag":[140],"until":[142],"synthesis,":[144,211],"such":[146],"experiments,":[147],"was":[148,284],"dictated":[149],"by":[150,273,286,290],"length":[152],"initial":[155,172],"exposure":[156,173],"plasma.":[158,190,308],"that":[162],"incubated":[164],"5":[168],"hr":[169,185,201,208,220],"during":[170],"point;":[179],"they":[180],"began":[181],"12":[184],"after":[186],"However,":[191],"a":[192,205,213,224],"population":[193,214],"treated":[196,215],"10":[200],"became":[202,221],"arrested":[203,222,232,245],"at":[204,223,233],"point":[206,225,236],"6":[207],"whereas":[212],"12-15":[219],"immediately":[226],"synthesis.":[229,252],"Cells":[230],"remained":[231],"this":[234],"latter":[235],"as":[238,240],"long":[239],"24":[241],"hr,":[242],"induced":[257],"immediate":[258],"phase":[263,302],"apparent":[266],"first-order":[267],"being":[271],"determined":[272],"concentration.":[276],"This":[277],"commitment":[279],"(transition)":[280],"blocked":[285],"cycloheximide":[287],"but":[288],"hydroxyurea.":[291],"Removal":[292],"hydroxyurea":[295],"allowed":[296],"synchronously":[303],"absence":[306]},"counts_by_year":[{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}
