{"id":"https://openalex.org/W1971907531","doi":"https://doi.org/10.1083/jcb.99.5.1678","title":"Differences in the behavior of cytoplasmic granules and lipid bodies during human lung mast cell degranulation.","display_name":"Differences in the behavior of cytoplasmic granules and lipid bodies during human lung mast cell degranulation.","publication_year":1984,"publication_date":"1984-11-01","ids":{"openalex":"https://openalex.org/W1971907531","doi":"https://doi.org/10.1083/jcb.99.5.1678","mag":"1971907531","pmid":"https://pubmed.ncbi.nlm.nih.gov/6436254","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113347"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.99.5.1678","doi":"https://doi.org/10.1083/jcb.99.5.1678","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.99.5.1678","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113347","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059154238","display_name":"Ann M. Dvo\u0159\u00e0k","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A M Dvorak","raw_affiliation_strings":["Harvard University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard University","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051875568","display_name":"Ilan Hammel","orcid":"https://orcid.org/0000-0001-5115-5940","observed_orcids":["https://orcid.org/0000-0001-5115-5940"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"I Hammel","raw_affiliation_strings":["Harvard University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard University","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065558462","display_name":"Edward S. Schulman","orcid":"https://orcid.org/0000-0003-2928-2665","observed_orcids":["https://orcid.org/0000-0003-2928-2665"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"E S Schulman","raw_affiliation_strings":["Harvard University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard University","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047213494","display_name":"Stephen P. Peters","orcid":"https://orcid.org/0000-0003-4176-7999","observed_orcids":["https://orcid.org/0000-0003-4176-7999"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S P Peters","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081046119","display_name":"Donald W. MacGlashan","orcid":"https://orcid.org/0000-0003-4525-0452","observed_orcids":["https://orcid.org/0000-0003-4525-0452"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D W MacGlashan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056666751","display_name":"Robert P. Schleimer","orcid":"https://orcid.org/0000-0002-8330-0097","observed_orcids":["https://orcid.org/0000-0002-8330-0097"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R P Schleimer","raw_affiliation_strings":["Medicine, Allergy Division"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medicine, Allergy Division","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075179719","display_name":"Harold H. Newball","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H H Newball","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007425341","display_name":"Kathryn Pyne","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Pyne","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109566972","display_name":"Harold F. Dvorak","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H F Dvorak","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111846072","display_name":"Lawrence M. Lichtenstein","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L M Lichtenstein","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.5952,"has_fulltext":false,"cited_by_count":80,"citation_normalized_percentile":{"value":0.8358083,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"99","issue":"5","first_page":"1678","last_page":"1687"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11346","display_name":"Mast cells and histamine","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11346","display_name":"Mast cells and histamine","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mast-cell-degranulation","display_name":"mast cell degranulation","score":0.8610000014305115},{"id":"https://openalex.org/keywords/cytoplasmic-granules","display_name":"cytoplasmic granules","score":0.9300000071525574},{"id":"https://openalex.org/keywords/lipid-droplets","display_name":"lipid droplets","score":0.8730000257492065},{"id":"https://openalex.org/keywords/lung-mast-cells","display_name":"lung mast cells","score":0.8740000128746033},{"id":"https://openalex.org/keywords/arachidonic-acid","display_name":"arachidonic acid","score":0.753000020980835},{"id":"https://openalex.org/keywords/histamine-release","display_name":"histamine release","score":0.8970000147819519},{"id":"https://openalex.org/keywords/anti-ige-stimulation","display_name":"anti-IgE stimulation","score":0.8809999823570251},{"id":"https://openalex.org/keywords/mast-cell-activation","display_name":"mast cell activation","score":0.6579999923706055},{"id":"https://openalex.org/keywords/ultrastructure","display_name":"ultrastructure","score":0.7239999771118164}],"concepts":[{"id":"https://openalex.org/C49802076","wikidata":"https://www.wikidata.org/wiki/Q5251810","display_name":"Degranulation","level":3,"score":0.945374608039856},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.8201577067375183},{"id":"https://openalex.org/C2779726688","wikidata":"https://www.wikidata.org/wiki/Q191989","display_name":"Mast cell","level":2,"score":0.7429856061935425},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7147064208984375},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.6928593516349792},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4969516098499298},{"id":"https://openalex.org/C87555872","wikidata":"https://www.wikidata.org/wiki/Q3141316","display_name":"Ultrastructure","level":2,"score":0.4689115285873413},{"id":"https://openalex.org/C25095133","wikidata":"https://www.wikidata.org/wiki/Q573950","display_name":"Lipid droplet","level":2,"score":0.4586307108402252},{"id":"https://openalex.org/C27239569","wikidata":"https://www.wikidata.org/wiki/Q189029","display_name":"Cell fractionation","level":3,"score":0.4232470989227295},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3820495903491974},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2121308147907257},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.20282918214797974},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0931675136089325},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000888","descriptor_name":"Antibodies, Anti-Idiotypic","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D001095","descriptor_name":"Arachidonic Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D003594","descriptor_name":"Cytoplasmic Granules","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D003594","descriptor_name":"Cytoplasmic Granules","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007073","descriptor_name":"Immunoglobulin E","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008168","descriptor_name":"Lung","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D008407","descriptor_name":"Mast Cells","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016718","descriptor_name":"Arachidonic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D050356","descriptor_name":"Lipid Metabolism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1083/jcb.99.5.1678","doi":"https://doi.org/10.1083/jcb.99.5.1678","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.99.5.1678","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2113347","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113347","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmid:6436254","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6436254","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.277.7195","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.277.7195","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/f8/93/J_Cell_Biol_1984_Nov_1_99(5)_1678-1687.tar.gz","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2113347","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113347","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W88011018","https://openalex.org/W91719421","https://openalex.org/W1251446275","https://openalex.org/W1677000101","https://openalex.org/W1876053957","https://openalex.org/W1919126881","https://openalex.org/W1922797677","https://openalex.org/W1991800163","https://openalex.org/W1994445058","https://openalex.org/W2016208025","https://openalex.org/W2020776617","https://openalex.org/W2027581588","https://openalex.org/W2036468682","https://openalex.org/W2051830137","https://openalex.org/W2061574141","https://openalex.org/W2069834296","https://openalex.org/W2085965811","https://openalex.org/W2150318178","https://openalex.org/W2173187023","https://openalex.org/W2266250640","https://openalex.org/W2414595141","https://openalex.org/W2417370582"],"related_works":["https://openalex.org/W7096309041","https://openalex.org/W153648831","https://openalex.org/W2415016028","https://openalex.org/W2416363537","https://openalex.org/W2093336596","https://openalex.org/W176071814","https://openalex.org/W317172391","https://openalex.org/W67090668","https://openalex.org/W2051999068","https://openalex.org/W2047254144"],"abstract_inverted_index":{"We":[0,90],"used":[1],"a":[2],"morphometric":[3],"and":[4,14,35,49,106,218,230],"autoradiographic":[5],"approach":[6],"to":[7,39,43,57,181,195],"analyze":[8],"changes":[9],"in":[10,103,147,178,227],"specific":[11,54,135,185],"cytoplasmic":[12,15,136,186,216,219],"granules":[13,137,217],"lipid":[16,93,114,144,162,220],"bodies":[17,115,145,163,221],"associated":[18],"with":[19,150,173],"human":[20],"lung":[21,30],"mast":[22,108,154,207,214,233],"cell":[23,155,208,215,234],"degranulation.":[24],"Mast":[25],"cells":[26,75],"were":[27,36,76,84,101],"dissociated":[28],"from":[29,51,123,128],"tissue":[31],"by":[32,45,64],"enzymatic":[33],"digestion":[34],"then":[37],"enriched":[38],"purities":[40],"of":[41,74,80,120,161,184,191,206],"up":[42,127],"99%":[44],"countercurrent":[46],"centrifugation":[47],"elutriation":[48],"recovery":[50],"columns":[52],"containing":[53],"antigen":[55],"bound":[56],"Sepharose":[58],"6":[59],"MB.":[60],"Degranulation":[61],"was":[62],"induced":[63],"goat":[65],"anti-IgE.":[66,174],"At":[67],"various":[68],"intervals":[69],"after":[70,171],"stimulation,":[71],"parallel":[72],"aliquots":[73],"recovered":[77],"for":[78,86],"determination":[79],"histamine":[81],"release":[82],"or":[83],"fixed":[85],"transmission":[87],"electron":[88],"microscopy.":[89],"found":[91],"that":[92,97,113,213,225],"bodies,":[94],"electron-dense":[95],"structures":[96],"lack":[98],"unit":[99],"membranes,":[100],"present":[102],"both":[104],"control":[105],"stimulated":[107],"cells.":[109],"Autoradiographic":[110],"analysis":[111],"showed":[112],"represented":[116],"the":[117,129,134,158,168,182,188,202],"major":[118],"repository":[119],"3H-label":[121],"derived":[122],"[3H]arachidonic":[124],"acid":[125],"taken":[126],"external":[130],"milieu.":[131],"By":[132],"contrast,":[133],"contained":[138],"no":[139],"detectable":[140],"3H-label.":[141],"In":[142],"addition,":[143],"occurred":[146],"intimate":[148],"association":[149],"degranulation":[151],"channels":[152],"during":[153,167,201,232],"activation,":[156],"but":[157],"total":[159],"volume":[160],"did":[164],"not":[165],"change":[166],"20":[169,204],"min":[170,205],"stimulation":[172],"This":[175],"result":[176],"stands":[177],"striking":[179],"contrast":[180],"behavior":[183,231],"granules,":[187],"great":[189],"majority":[190],"which":[192],"(77%":[193],"according":[194],"aggregate":[196],"volume)":[197],"exhibited":[198],"ultrastructural":[199],"alterations":[200],"first":[203],"activation.":[209,235],"These":[210],"observations":[211],"establish":[212],"are":[222],"distinct":[223],"organelles":[224],"differ":[226],"ultrastructure,":[228],"biochemistry,":[229]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}