{"id":"https://openalex.org/W1971481340","doi":"https://doi.org/10.1186/1472-6750-11-22","title":"Utilizing high-throughput experimentation to enhance specific productivity of an E.coliT7 expression system by phosphate limitation","display_name":"Utilizing high-throughput experimentation to enhance specific productivity of an E.coliT7 expression system by phosphate limitation","publication_year":2011,"publication_date":"2011-03-17","ids":{"openalex":"https://openalex.org/W1971481340","doi":"https://doi.org/10.1186/1472-6750-11-22","mag":"1971481340","pmid":"https://pubmed.ncbi.nlm.nih.gov/21414195","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3068942"},"language":"en","primary_location":{"id":"doi:10.1186/1472-6750-11-22","doi":"https://doi.org/10.1186/1472-6750-11-22","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1472-6750-11-22","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-11-22","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-11-22","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067902608","display_name":"Robert Huber","orcid":"https://orcid.org/0000-0002-0543-8998","observed_orcids":["https://orcid.org/0000-0002-0543-8998"]},"institutions":[{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Robert Huber","raw_affiliation_strings":["RWTH Aachen University, Worringerweg 1, D-52074 Aachen, Germany. robert.huber@sandoz.com","AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"RWTH Aachen University, Worringerweg 1, D-52074 Aachen, Germany. robert.huber@sandoz.com","institution_ids":["https://openalex.org/I887968799"]},{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060977701","display_name":"Simon Roth","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Simon Roth","raw_affiliation_strings":["AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany","AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]},{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043316844","display_name":"Natalie Rahmen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Natalie Rahmen","raw_affiliation_strings":["AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany","AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]},{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022603631","display_name":"Jochen B\u00fcchs","orcid":"https://orcid.org/0000-0002-2012-3476","observed_orcids":["https://orcid.org/0000-0002-2012-3476"]},"institutions":[{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Jochen B\u00fcchs","raw_affiliation_strings":["AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany","AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52074, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]},{"raw_affiliation_string":"AVT - Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany","institution_ids":["https://openalex.org/I887968799"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5022603631","https://openalex.org/A5067902608"],"corresponding_institution_ids":["https://openalex.org/I887968799"],"apc_list":{"value":2990,"currency":"USD","value_usd":2990},"apc_paid":{"value":2990,"currency":"USD","value_usd":2990},"fwci":1.7119,"has_fulltext":true,"cited_by_count":59,"citation_normalized_percentile":{"value":0.83481647,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"11","issue":"1","first_page":"22","last_page":"22"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5595999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5595999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.14669999480247498,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.066600002348423,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phosphate-starvation","display_name":"phosphate limitation","score":0.7590000033378601},{"id":"https://openalex.org/keywords/t7-expression-system","display_name":"T7 expression system","score":0.8460000157356262},{"id":"https://openalex.org/keywords/escherichia-coli-bl21-de3","display_name":"Escherichia coli BL21(DE3)","score":0.9309999942779541},{"id":"https://openalex.org/keywords/fluorescent-protein-expression","display_name":"fluorescent protein expression","score":0.7210000157356262},{"id":"https://openalex.org/keywords/specific-productivity","display_name":"specific productivity","score":0.8759999871253967},{"id":"https://openalex.org/keywords/high-throughput-experimentation","display_name":"high-throughput experimentation","score":0.7680000066757202},{"id":"https://openalex.org/keywords/biolector","display_name":"BioLector","score":0.925000011920929},{"id":"https://openalex.org/keywords/ramos","display_name":"Ramos","score":0.6510000228881836},{"id":"https://openalex.org/keywords/iptg-induction","display_name":"IPTG induction","score":0.6740000247955322},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7879999876022339}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7334667444229126},{"id":"https://openalex.org/C204983608","wikidata":"https://www.wikidata.org/wiki/Q2111958","display_name":"Productivity","level":2,"score":0.5391237139701843},{"id":"https://openalex.org/C139149728","wikidata":"https://www.wikidata.org/wiki/Q335201","display_name":"Laboratory flask","level":2,"score":0.5284851789474487},{"id":"https://openalex.org/C115540264","wikidata":"https://www.wikidata.org/wiki/Q2945560","display_name":"Biomass (ecology)","level":2,"score":0.5001821517944336},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.4994621276855469},{"id":"https://openalex.org/C2777132085","wikidata":"https://www.wikidata.org/wiki/Q46220103","display_name":"Phosphate","level":2,"score":0.46500974893569946},{"id":"https://openalex.org/C2778810118","wikidata":"https://www.wikidata.org/wiki/Q534260","display_name":"Microtiter plate","level":2,"score":0.4393872022628784},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.4225248694419861},{"id":"https://openalex.org/C6856738","wikidata":"https://www.wikidata.org/wiki/Q331672","display_name":"Protein expression","level":3,"score":0.41582193970680237},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3835272192955017},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.376972496509552},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3737007975578308},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3555799424648285},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.32844066619873047},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.23114746809005737},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2222614884376526},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.135599285364151},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12817758321762085},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.10975953936576843},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.08342635631561279},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015964","descriptor_name":"Gene Expression Regulation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057166","descriptor_name":"High-Throughput Screening Assays","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D057166","descriptor_name":"High-Throughput Screening Assays","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1186/1472-6750-11-22","doi":"https://doi.org/10.1186/1472-6750-11-22","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1472-6750-11-22","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-11-22","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},{"id":"pmid:21414195","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21414195","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC biotechnology","raw_type":"Evaluation Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:3068942","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3068942/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:d0837b4779954828893d569b2d1f6c29","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/d0837b4779954828893d569b2d1f6c29","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Biotechnology, Vol 11, Iss 1, p 22 (2011)","raw_type":"article"},{"id":"pmh:oai:publications.rwth-aachen.de:184281","doi":null,"is_oa":true,"landing_page_url":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-060804%22","pdf_url":null,"source":{"id":"https://openalex.org/S4306401362","display_name":"RWTH Publications (RWTH Aachen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I887968799","host_organization_name":"RWTH Aachen University","host_organization_lineage":["https://openalex.org/I887968799"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC biotechnology 11(1), 22 (2011). doi:10.1186/1472-6750-11-22","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1186/1472-6750-11-22","doi":"https://doi.org/10.1186/1472-6750-11-22","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1472-6750-11-22","pdf_url":"https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-11-22","source":{"id":"https://openalex.org/S53489610","display_name":"BMC Biotechnology","issn_l":"1472-6750","issn":["1472-6750"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1971481340.pdf","grobid_xml":"https://content.openalex.org/works/W1971481340.grobid-xml"},"referenced_works_count":46,"referenced_works":["https://openalex.org/W38139084","https://openalex.org/W53741792","https://openalex.org/W1494958945","https://openalex.org/W1512810258","https://openalex.org/W1610001324","https://openalex.org/W1914467681","https://openalex.org/W1974809350","https://openalex.org/W1989903511","https://openalex.org/W1989974224","https://openalex.org/W1991383594","https://openalex.org/W2010774343","https://openalex.org/W2021201527","https://openalex.org/W2022057965","https://openalex.org/W2023392129","https://openalex.org/W2028549151","https://openalex.org/W2045439749","https://openalex.org/W2051830256","https://openalex.org/W2064746239","https://openalex.org/W2077776831","https://openalex.org/W2078523486","https://openalex.org/W2081544371","https://openalex.org/W2083315765","https://openalex.org/W2084599950","https://openalex.org/W2087587733","https://openalex.org/W2102454474","https://openalex.org/W2106493349","https://openalex.org/W2106943551","https://openalex.org/W2110039658","https://openalex.org/W2110693589","https://openalex.org/W2113141287","https://openalex.org/W2120479418","https://openalex.org/W2122952856","https://openalex.org/W2124220997","https://openalex.org/W2133699631","https://openalex.org/W2133903524","https://openalex.org/W2144340372","https://openalex.org/W2145907180","https://openalex.org/W2148325536","https://openalex.org/W2149079511","https://openalex.org/W2150686726","https://openalex.org/W2161263507","https://openalex.org/W2164306370","https://openalex.org/W2165183373","https://openalex.org/W2166361214","https://openalex.org/W2267880147","https://openalex.org/W2423849579"],"related_works":["https://openalex.org/W4223577114","https://openalex.org/W7134939326","https://openalex.org/W1744247774","https://openalex.org/W6939031374","https://openalex.org/W2769232385","https://openalex.org/W3026552519","https://openalex.org/W3026425396","https://openalex.org/W3195372482","https://openalex.org/W7009948624","https://openalex.org/W4390609568"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,166,194,254],"specific":[2,123,143,230],"productivity":[3,124,231],"of":[4,16,19,42,86,94,112,125,189,199,241,245,307,325],"cultivation":[5,157,275],"processes":[6],"can":[7,207],"be":[8,99],"optimized,":[9],"amongst":[10],"others,":[11],"by":[12,48],"using":[13,214],"genetic":[14],"engineering":[15],"strains,":[17],"choice":[18],"suitable":[20,267],"host/vector":[21],"systems":[22],"or":[23],"process":[24,164],"optimization":[25],"(e.g.":[26,62],"choosing":[27],"the":[28,53,84,92,110,122,126,135,170,178,187,222,229,246,262,270,293,305,308,322],"right":[29,263],"induction":[30,114,224,264],"time).":[31],"A":[32],"further":[33,320],"possibility":[34],"is":[35,70,196],"to":[36,73,98,182,220,227,239,268,276,315,319],"reduce":[37],"biomass":[38,282],"buildup":[39],"in":[40,52,66,101,174,191,281],"favor":[41],"an":[43,288,297],"enhanced":[44],"product":[45],"formation,":[46],"e.g.":[47],"limiting":[49],"secondary":[50],"substrates":[51],"medium,":[54],"such":[55,287],"as":[56,83,152,154],"phosphate.":[57],"However,":[58],"with":[59],"conventional":[60],"techniques":[61],"small":[63,147],"scale":[64,148],"cultivations":[65,149],"shake":[67,175],"flasks),":[68],"it":[69,217],"very":[71],"tedious":[72],"establish":[74],"optimal":[75,223],"conditions":[76],"for":[77,146,162,232,292,296],"cell":[78],"growth":[79,185],"and":[80,91,116,186,202,206,226,304],"protein":[81,87,138,243,277],"expression,":[82],"start":[85],"expression":[88,128,278,299],"(induction":[89],"time)":[90],"degree":[93],"phosphate":[95,119,251,259],"limitation":[96,120,260],"have":[97],"determined":[100],"numerous":[102],"concerted,":[103],"manually":[104],"conducted":[105],"experiments.":[106],"RESULTS:":[107],"We":[108],"investigated":[109],"effect":[111,289],"different":[113],"times":[115],"a":[117,155,197,200,203,250,258],"concurrent":[118],"on":[121],"T7":[127],"system":[129,168],"E.coli":[130,247],"BL21(DE3)":[131],"pRhotHi-2-EcFbFP,":[132],"which":[133],"produces":[134],"model":[136],"fluorescence":[137],"EcFbFP":[139,190,233],"upon":[140],"induction.":[141],"Therefore,":[142],"online-monitoring":[144],"tools":[145],"(RAMOS,":[150],"BioLector)":[151],"well":[153],"novel":[156],"platform":[158],"(Robo-Lector)":[159],"were":[160],"used":[161],"rapid":[163],"optimization.":[165],"RAMOS":[167],"monitored":[169],"oxygen":[171],"transfer":[172],"rate":[173],"flasks,":[176],"whereas":[177],"BioLector":[179,201],"device":[180],"allowed":[181],"monitor":[183],"microbial":[184],"production":[188,323],"microtiter":[192],"plates.":[193],"Robo-Lector":[195],"combination":[198],"pipetting":[204],"robot":[205],"conduct":[208],"high-throughput":[209,310],"experiments":[210],"fully":[211],"automated.":[212],"By":[213],"these":[215],"tools,":[216],"was":[218,266,290],"possible":[219],"determine":[221],"time":[225,265,295],"increase":[228],"from":[234],"22%":[235],"(for":[236],"unlimited":[237],"conditions)":[238],"31%":[240],"total":[242],"content":[244],"cells":[248],"via":[249],"limitation.":[252],"CONCLUSIONS:":[253],"results":[255],"revealed":[256],"that":[257],"at":[261],"redirect":[269],"available":[271],"cellular":[272],"resources":[273],"during":[274],"rather":[279],"than":[280],"production.":[283],"To":[284],"our":[285],"knowledge,":[286],"shown":[291],"first":[294],"IPTG-inducible":[298],"system.":[300],"Finally,":[301],"this":[302],"finding":[303],"utilization":[306],"introduced":[309],"experimentation":[311],"approach":[312],"could":[313],"help":[314],"find":[316],"new":[317],"targets":[318],"enhance":[321],"capacity":[324],"recombinant":[326],"E.coli-strains.":[327]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":4},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}