{"id":"https://openalex.org/W1970459848","doi":"https://doi.org/10.1083/jcb.100.3.692","title":"Tropomyosin isoforms in chicken embryo fibroblasts: purification, characterization, and changes in Rous sarcoma virus-transformed cells.","display_name":"Tropomyosin isoforms in chicken embryo fibroblasts: purification, characterization, and changes in Rous sarcoma virus-transformed cells.","publication_year":1985,"publication_date":"1985-03-01","ids":{"openalex":"https://openalex.org/W1970459848","doi":"https://doi.org/10.1083/jcb.100.3.692","mag":"1970459848","pmid":"https://pubmed.ncbi.nlm.nih.gov/2982883","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113520"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.100.3.692","doi":"https://doi.org/10.1083/jcb.100.3.692","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.100.3.692","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113520","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060436847","display_name":"Jing\u2010Jer Lin","orcid":"https://orcid.org/0000-0001-8250-7398","observed_orcids":["https://orcid.org/0000-0001-8250-7398"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J J Lin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039994535","display_name":"David M. Helfman","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D M Helfman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063432648","display_name":"Stephen H. Hughes","orcid":"https://orcid.org/0000-0002-9176-4377","observed_orcids":["https://orcid.org/0000-0002-9176-4377"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S H Hughes","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"C S Chou","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C S Chou","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":5.4757,"has_fulltext":false,"cited_by_count":122,"citation_normalized_percentile":{"value":0.96503885,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"100","issue":"3","first_page":"692","last_page":"703"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9247999787330627,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9247999787330627,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10882","display_name":"Cardiomyopathy and Myosin Studies","score":0.025599999353289604,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.014000000432133675,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tropomyosin-isoforms","display_name":"tropomyosin isoforms","score":0.9980000257492065},{"id":"https://openalex.org/keywords/chicken-embryo-fibroblasts","display_name":"chicken embryo fibroblasts","score":0.996999979019165},{"id":"https://openalex.org/keywords/actin-binding-proteins","display_name":"actin-binding proteins","score":0.6990000009536743},{"id":"https://openalex.org/keywords/heterodimer","display_name":"heterodimer","score":0.5730000138282776},{"id":"https://openalex.org/keywords/actin-filaments","display_name":"F-actin","score":0.5649999976158142},{"id":"https://openalex.org/keywords/actin-cytoskeleton","display_name":"actin cytoskeleton","score":0.4959999918937683},{"id":"https://openalex.org/keywords/in-vitro-translation","display_name":"in vitro translation","score":0.4269999861717224},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C205875245","wikidata":"https://www.wikidata.org/wiki/Q418454","display_name":"Tropomyosin","level":3,"score":0.9734323024749756},{"id":"https://openalex.org/C2777441520","wikidata":"https://www.wikidata.org/wiki/Q1444330","display_name":"Rous sarcoma virus","level":3,"score":0.7200027108192444},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7139953374862671},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.6840134263038635},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.5437913537025452},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5269169211387634},{"id":"https://openalex.org/C2779546866","wikidata":"https://www.wikidata.org/wiki/Q531306","display_name":"Dimer","level":2,"score":0.5051096081733704},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4367597699165344},{"id":"https://openalex.org/C74884574","wikidata":"https://www.wikidata.org/wiki/Q171407","display_name":"Divalent","level":2,"score":0.4327367842197418},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.19684338569641113},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0778876543045044},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001358","descriptor_name":"Avian Sarcoma Viruses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002472","descriptor_name":"Cell Transformation, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002642","descriptor_name":"Chick Embryo","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014335","descriptor_name":"Tropomyosin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014335","descriptor_name":"Tropomyosin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1083/jcb.100.3.692","doi":"https://doi.org/10.1083/jcb.100.3.692","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.100.3.692","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2113520","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113520","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmid:2982883","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2982883","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.277.6540","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.277.6540","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/43/8d/J_Cell_Biol_1985_Mar_1_100(3)_692-703.tar.gz","raw_type":"text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.325.9257","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.325.9257","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://jcb.rupress.org/content/100/3/692.full.pdf","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2113520","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2113520","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1383079005","display_name":null,"funder_award_id":"GM 34105","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":57,"referenced_works":["https://openalex.org/W86089617","https://openalex.org/W1481088618","https://openalex.org/W1513911231","https://openalex.org/W1538692246","https://openalex.org/W1544906997","https://openalex.org/W1544945960","https://openalex.org/W1550194011","https://openalex.org/W1550621158","https://openalex.org/W1564857046","https://openalex.org/W1594601131","https://openalex.org/W1595174870","https://openalex.org/W1602949099","https://openalex.org/W1606423922","https://openalex.org/W1614508806","https://openalex.org/W1751103227","https://openalex.org/W1775749144","https://openalex.org/W1803519053","https://openalex.org/W1936818573","https://openalex.org/W1966889209","https://openalex.org/W1970264680","https://openalex.org/W1970403529","https://openalex.org/W1973887565","https://openalex.org/W1974820795","https://openalex.org/W1975018292","https://openalex.org/W1979533640","https://openalex.org/W1980287746","https://openalex.org/W1982025705","https://openalex.org/W1998031179","https://openalex.org/W2001327688","https://openalex.org/W2002906547","https://openalex.org/W2008463384","https://openalex.org/W2018195612","https://openalex.org/W2018289835","https://openalex.org/W2020310775","https://openalex.org/W2034277598","https://openalex.org/W2034634699","https://openalex.org/W2042678076","https://openalex.org/W2049238454","https://openalex.org/W2053295194","https://openalex.org/W2060161407","https://openalex.org/W2060333964","https://openalex.org/W2063470561","https://openalex.org/W2066160869","https://openalex.org/W2066541577","https://openalex.org/W2076307165","https://openalex.org/W2081595292","https://openalex.org/W2086176683","https://openalex.org/W2100837269","https://openalex.org/W2133315219","https://openalex.org/W2133769506","https://openalex.org/W2149787772","https://openalex.org/W2155254731","https://openalex.org/W2166691181","https://openalex.org/W2171718050","https://openalex.org/W2174180683","https://openalex.org/W2218823496","https://openalex.org/W2345608927"],"related_works":["https://openalex.org/W7095189774","https://openalex.org/W2171042004","https://openalex.org/W7095799349","https://openalex.org/W2174180683","https://openalex.org/W2036927953","https://openalex.org/W1525081901","https://openalex.org/W2001327688","https://openalex.org/W2049492301","https://openalex.org/W1954362474","https://openalex.org/W4254499792"],"abstract_inverted_index":{"Seven":[0],"polypeptides":[1],"(a,":[2],"b,":[3],"c,":[4],"1,":[5,155,200],"2,":[6,156],"3a,":[7],"and":[8,27,37,48,68,93,157,170,201,217,272,303,313,348],"3b)":[9,184],"have":[10,293],"been":[11,80,262],"previously":[12],"identified":[13],"as":[14],"tropomyosin":[15,62,78,100,114,146,180,194,199,215,232,242,252,284,301],"isoforms":[16,59,115,147,253,336],"in":[17,119,149,163,228,254,289,307,331,338,355],"chicken":[18,53,66],"embryo":[19],"fibroblasts":[20],"(CEF)":[21],"(Lin,":[22],"J.":[23,31],"J.-C.,":[24],"Matsumura,":[25],"F.,":[26],"Yamashiro-Matsumura,":[28],"S.,":[29],"1984,":[30],"Cell.":[32],"Biol.,":[33],"98:116-127).":[34],"Spots":[35],"a":[36,216,220],"c":[38,218],"had":[39],"identical":[40,111],"mobility":[41],"on":[42,71],"two-dimensional":[43,72],"gels":[44],"with":[45,212,240,245,266],"the":[46,57,83,120,124,150,164,177,227,267,297,309,314,322,332,343,349],"slow-migrating":[47],"fast-migrating":[49],"components,":[50],"respectively,":[51],"of":[52,60,76,85,88,99,106,113,128,166,179,193,231,250,300,317,321,334,345,351],"gizzard":[54],"tropomyosin.":[55,247],"However,":[56],"remaining":[58],"CEF":[61,77,108,134,198,258,328],"were":[63,305],"distinct":[64],"from":[65,133],"skeletal":[67],"cardiac":[69],"tropomyosins":[70,153],"gels.":[73],"The":[74,97,110,248],"mixture":[75],"has":[79,260],"isolated":[81,132],"by":[82,210,270],"combination":[84],"Triton/glycerol":[86],"extraction":[87],"monolayer":[89],"cells,":[90],"heat":[91],"treatment,":[92],"ammonium":[94],"sulfate":[95],"fractionation.":[96],"yield":[98],"was":[101,188,286],"estimated":[102],"to":[103,185,207],"be":[104,117,205,340],"1.4%":[105],"total":[107,129,310],"proteins.":[109],"set":[112],"could":[116,204],"found":[118,281,295,354],"antitropomyosin":[121],"immunoprecipitates":[122],"after":[123],"cell-free":[125],"translation":[126],"products":[127],"poly(A)+":[130],"RNAs":[131],"cells.":[135,291,319,357],"This":[136],"suggested":[137],"that":[138,192,282,296],"at":[139],"least":[140],"seven":[141],"mRNAs":[142],"coding":[143],"for":[144,327,342],"these":[145,251,335],"existed":[148],"cell.":[151],"Purified":[152],"(particularly":[154],"3)":[158],"showed":[159],"different":[160,323],"actin-binding":[161,324],"abilities":[162],"presence":[165],"100":[167],"mM":[168],"KCl":[169],"no":[171],"divalent":[172],"cation.":[173],"Under":[174],"this":[175],"condition,":[176],"binding":[178],"3":[181],"(3a":[182],"+":[183],"actin":[186,346],"filaments":[187],"significantly":[189],"weaker":[190],"than":[191],"1":[195,285],"or":[196],"2.":[197],"probably":[202],"3,":[203],"cross-linked":[206],"form":[208],"homodimers":[209],"treatment":[211],"5,5'-dithiobis-(2-nitrobenzoate),":[213],"whereas":[214],"formed":[219],"heterodimer.":[221],"These":[222],"dimer":[223,235],"species":[224],"may":[225],"reflect":[226],"vivo":[229],"assembly":[230],"isoforms,":[233],"since":[234],"formation":[236],"occurred":[237],"not":[238],"only":[239],"purified":[241],"but":[243],"also":[244,261,294],"microfilament-associated":[246],"expression":[249,333],"Rous":[255],"sarcoma":[256],"virus-transformed":[257],"cells":[259],"investigated.":[263],"In":[264],"agreement":[265],"previous":[268],"report":[269],"Hendricks":[271],"Weintraub":[273],"(Proc.":[274],"Natl.":[275],"Acad.":[276],"Sci.":[277],"USA.,":[278],"78:5633-5637),":[279],"we":[280],"major":[283],"greatly":[287],"reduced":[288],"transformed":[290,318,356],"We":[292],"relative":[298],"amounts":[299],"3a":[302],"3b":[304],"increased":[306],"both":[308],"cell":[311,352],"lysate":[312],"microfilament":[315],"fraction":[316],"Because":[320],"properties":[325],"observed":[326],"tropomyosins,":[329],"changes":[330],"may,":[337],"part,":[339],"responsible":[341],"reduction":[344],"cables":[347],"alteration":[350],"shape":[353]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}