{"id":"https://openalex.org/W1970405407","doi":"https://doi.org/10.1080/10611860600907767","title":"A gene delivery approach for antimicrobials: Expression of defensins","display_name":"A gene delivery approach for antimicrobials: Expression of defensins","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W1970405407","doi":"https://doi.org/10.1080/10611860600907767","mag":"1970405407","pmid":"https://pubmed.ncbi.nlm.nih.gov/17090400"},"language":"en","primary_location":{"id":"doi:10.1080/10611860600907767","doi":"https://doi.org/10.1080/10611860600907767","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10611860600907767","pdf_url":null,"source":{"id":"https://openalex.org/S100629371","display_name":"Journal of drug targeting","issn_l":"1026-7158","issn":["1026-7158","1029-2330","1061-186X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Drug Targeting","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Chun Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chun Zhang","raw_affiliation_strings":["Department of Pharmaceutics, University of Florida, Gainesville, FL 32610, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics, University of Florida, Gainesville, FL 32610, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072300066","display_name":"Preeti Yadava","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Preeti Yadava","raw_affiliation_strings":["Department of Pharmaceutics, University of Florida, Gainesville, FL32610, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics, University of Florida, Gainesville, FL32610, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103282310","display_name":"Jing Ya Sun","orcid":"https://orcid.org/0000-0003-2040-1066","observed_orcids":["https://orcid.org/0000-0003-2040-1066"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jing Sun","raw_affiliation_strings":["Department of Pharmaceutics, University of Florida, Gainesville, FL32610, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics, University of Florida, Gainesville, FL32610, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103689052","display_name":"Jeffrey A. Hughes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey A Hughes","raw_affiliation_strings":["Department of Pharmaceutics, University of Florida, Gainesville, FL32610, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutics, University of Florida, Gainesville, FL32610, USA","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":null,"apc_paid":null,"fwci":0.5936,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.69121067,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"14","issue":"9","first_page":"646","last_page":"651"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.8978999853134155,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.8978999853134155,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.09860000014305115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-beta-defensin-3","display_name":"human beta-defensin 3","score":0.8550000190734863},{"id":"https://openalex.org/keywords/antimicrobial-peptides","display_name":"antimicrobial peptides","score":0.7620000243186951},{"id":"https://openalex.org/keywords/peptide-antibiotics","display_name":"peptide antibiotics","score":0.7089999914169312},{"id":"https://openalex.org/keywords/gene-delivery","display_name":"gene delivery","score":0.8389999866485596},{"id":"https://openalex.org/keywords/polyethylenimine-transfection","display_name":"polyethylenimine transfection","score":0.9100000262260437},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.9300000071525574},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7360000014305115},{"id":"https://openalex.org/keywords/bacillus","display_name":"Bacillus","score":0.996999979019165},{"id":"https://openalex.org/keywords/peptide-secretion","display_name":"peptide secretion","score":0.6330000162124634},{"id":"https://openalex.org/keywords/antifungal-activity","display_name":"antifungal activity","score":0.4009999930858612}],"concepts":[{"id":"https://openalex.org/C2776498113","wikidata":"https://www.wikidata.org/wiki/Q412820","display_name":"Defensin","level":3,"score":0.8243116140365601},{"id":"https://openalex.org/C540938839","wikidata":"https://www.wikidata.org/wiki/Q1201508","display_name":"Antimicrobial peptides","level":3,"score":0.689146876335144},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6619030237197876},{"id":"https://openalex.org/C129437159","wikidata":"https://www.wikidata.org/wiki/Q4897294","display_name":"Beta defensin","level":4,"score":0.6043800115585327},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5853458642959595},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5680622458457947},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5501466989517212},{"id":"https://openalex.org/C2775925850","wikidata":"https://www.wikidata.org/wiki/Q418697","display_name":"Polyethylenimine","level":4,"score":0.5261563658714294},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.501000165939331},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4451333284378052},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.44015786051750183},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.43491101264953613},{"id":"https://openalex.org/C135983454","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Gene delivery","level":4,"score":0.41219577193260193},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3799920380115509},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3031597435474396},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19821128249168396}],"mesh":[{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005079","descriptor_name":"Excipients","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011094","descriptor_name":"Polyethyleneimine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016503","descriptor_name":"Drug Delivery Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023082","descriptor_name":"Defensins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D023082","descriptor_name":"Defensins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D023083","descriptor_name":"beta-Defensins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D023083","descriptor_name":"beta-Defensins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1080/10611860600907767","doi":"https://doi.org/10.1080/10611860600907767","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10611860600907767","pdf_url":null,"source":{"id":"https://openalex.org/S100629371","display_name":"Journal of drug targeting","issn_l":"1026-7158","issn":["1026-7158","1029-2330","1061-186X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Drug Targeting","raw_type":"journal-article"},{"id":"pmid:17090400","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17090400","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of drug targeting","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8999999761581421,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W1479939900","https://openalex.org/W1512592922","https://openalex.org/W1571292455","https://openalex.org/W1593827001","https://openalex.org/W1971891360","https://openalex.org/W1981045116","https://openalex.org/W1988655657","https://openalex.org/W1998630678","https://openalex.org/W2013245073","https://openalex.org/W2030642888","https://openalex.org/W2041971745","https://openalex.org/W2042746016","https://openalex.org/W2049358414","https://openalex.org/W2058223359","https://openalex.org/W2060306551","https://openalex.org/W2063951978","https://openalex.org/W2065766479","https://openalex.org/W2073313072","https://openalex.org/W2078918986","https://openalex.org/W2090042814","https://openalex.org/W2104630130","https://openalex.org/W2107735101","https://openalex.org/W2122872625","https://openalex.org/W2124385642","https://openalex.org/W2128590692","https://openalex.org/W2136538608","https://openalex.org/W2138207315","https://openalex.org/W2148067829","https://openalex.org/W2158789628","https://openalex.org/W2167674475","https://openalex.org/W2179183038","https://openalex.org/W2292182586","https://openalex.org/W2328438661"],"related_works":["https://openalex.org/W2366600980","https://openalex.org/W2502286664","https://openalex.org/W1975966384","https://openalex.org/W1780502235","https://openalex.org/W2363324553","https://openalex.org/W1989398510","https://openalex.org/W2388570509","https://openalex.org/W2164285431","https://openalex.org/W7096297002","https://openalex.org/W2013245073"],"abstract_inverted_index":{"INTRODUCTION:":[0],"Peptide":[1],"antibiotics":[2],"as":[3,113,128,161,163],"new":[4,28],"therapeutic":[5],"agents":[6],"are":[7],"becoming":[8],"a":[9,50,64,70,119],"popular":[10],"option":[11],"to":[12,15,84],"investigate":[13],"due":[14],"their":[16],"broad":[17],"bacterial":[18],"target":[19],"selectivity":[20],"and":[21,37,117,147],"limited":[22],"resistance":[23],"problems.":[24],"Although":[25],"attractive,":[26],"these":[27],"drug":[29],"candidates":[30],"have":[31],"several":[32],"limitations":[33],"including":[34],"low":[35],"potency":[36],"delivery":[38],"issues":[39],"which":[40],"face":[41],"all":[42],"peptides/proteins.":[43],"METHODS:":[44],"In":[45],"this":[46],"study,":[47],"we":[48],"designed":[49],"plasmid":[51,77],"expression":[52,73,76,104],"system":[53],"for":[54,81],"human":[55,65],"beta":[56,120],"defensin":[57,87,121],"3.":[58],"This":[59,75],"sequence":[60],"was":[61,78,106,123,139],"cloned":[62],"from":[63],"epithelial":[66],"lung":[67],"cell":[68,166],"into":[69],"CMV":[71],"driven":[72],"cassette.":[74],"then":[79],"evaluated":[80],"its":[82],"ability":[83],"produce":[85],"human-beta":[86],"3":[88],"with":[89],"the":[90,93,103,126,137,158,164,172],"use":[91],"of":[92,136],"non-viral":[94],"transfection":[95],"agent,":[96],"polyethylenimine":[97],"(PEI).":[98],"RESULTS:":[99],"The":[100,133],"results":[101],"indicate":[102],"cassette":[105],"transcriptionally":[107],"active":[108],"in":[109,153],"HEK":[110],"293":[111],"cells,":[112],"measured":[114],"by":[115,125,130],"RT-PCR":[116],"that":[118],"peptide":[122,138,173],"produced":[124],"cells":[127],"confirmed":[129,140],"Western":[131],"blot.":[132],"biological":[134,169],"activity":[135,170],"against":[141],"both":[142],"gram":[143,148],"negative":[144],"E.":[145],"coli":[146],"positive":[149],"Bacillus":[150],"species":[151],"using":[152],"vitro":[154],"screening.":[155],"CONCLUSION:":[156],"Both":[157],"cultured":[159],"media":[160],"well":[162],"transfected":[165],"lysate":[167],"demonstrated":[168],"demonstrating":[171],"is":[174],"also":[175],"secreted.":[176]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}