{"id":"https://openalex.org/W1969458451","doi":"https://doi.org/10.1093/nar/18.19.5637","title":"Sse8387I, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5\u2032-CCTGCAGG-3\u2032","display_name":"Sse8387I, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5\u2032-CCTGCAGG-3\u2032","publication_year":1990,"publication_date":"1990-01-01","ids":{"openalex":"https://openalex.org/W1969458451","doi":"https://doi.org/10.1093/nar/18.19.5637","mag":"1969458451","pmid":"https://pubmed.ncbi.nlm.nih.gov/2170941","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/332294"},"language":"en","primary_location":{"id":"doi:10.1093/nar/18.19.5637","doi":"https://doi.org/10.1093/nar/18.19.5637","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/18.19.5637","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC332294","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Hirokazu Kotani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hirokazu Kotani","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd., Shiga, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd., Shiga, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yoshiko Nomura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshiko Nomura","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yukiko Kawashima","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yukiko Kawashima","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110698229","display_name":"Hiroaki Sagawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroaki Sagawa","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108685205","display_name":"Makoto Takagi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Makoto Takagi","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075515746","display_name":"Akihiko Kita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akihiko Kita","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109078365","display_name":"Hiroaki Ito","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroaki Ito","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110168747","display_name":"Ikunosin Kato","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405273692","display_name":"Takara Shuzo Co., Ltd. (Japan)","ror":"https://ror.org/009xcqz70","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405273692"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ikunosin Kato","raw_affiliation_strings":["Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioproducts Development and Research Center, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu, Shiga, 520-21, Japan","institution_ids":["https://openalex.org/I4405273692"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4405273692"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":0.598,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.60631823,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"18","issue":"19","first_page":"5637","last_page":"5640"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8877000212669373,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8877000212669373,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.04899999871850014,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.017799999564886093,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/type-ii-restriction-endonuclease","display_name":"type II restriction endonuclease","score":0.9929999709129333}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9016138315200806},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.8252314329147339},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6097413301467896},{"id":"https://openalex.org/C205225487","wikidata":"https://www.wikidata.org/wiki/Q287604","display_name":"PBR322","level":4,"score":0.5663689374923706},{"id":"https://openalex.org/C19014433","wikidata":"https://www.wikidata.org/wiki/Q5130995","display_name":"Cleave","level":3,"score":0.559781014919281},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5569526553153992},{"id":"https://openalex.org/C100594029","wikidata":"https://www.wikidata.org/wiki/Q7302658","display_name":"Recognition sequence","level":4,"score":0.5269221663475037},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.5139069557189941},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5128124356269836},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.4921228289604187},{"id":"https://openalex.org/C84289048","wikidata":"https://www.wikidata.org/wiki/Q7316314","display_name":"Restriction fragment","level":3,"score":0.4850834906101227},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.47306978702545166},{"id":"https://openalex.org/C170673058","wikidata":"https://www.wikidata.org/wiki/Q780686","display_name":"Restriction map","level":4,"score":0.4569111764431},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.42533713579177856},{"id":"https://openalex.org/C2781135666","wikidata":"https://www.wikidata.org/wiki/Q615868","display_name":"Lambda phage","level":5,"score":0.4102446436882019},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.2967221140861511},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.25051814317703247},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.23574012517929077},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.13950669765472412}],"mesh":[{"descriptor_ui":"D000260","descriptor_name":"Adenoviruses, Human","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001435","descriptor_name":"Bacteriophages","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009838","descriptor_name":"Oligodeoxyribonucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010582","descriptor_name":"Bacteriophage lambda","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013539","descriptor_name":"Simian virus 40","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015252","descriptor_name":"Deoxyribonucleases, Type II Site-Specific","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/18.19.5637","doi":"https://doi.org/10.1093/nar/18.19.5637","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/18.19.5637","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:42275","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC332294","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:332294","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/332294","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:2170941","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2170941","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:42275","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC332294","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W962031771","https://openalex.org/W1571644961","https://openalex.org/W1972072721","https://openalex.org/W2009556993","https://openalex.org/W2013404388","https://openalex.org/W2023829419","https://openalex.org/W2028622989","https://openalex.org/W2042694802","https://openalex.org/W2078958918","https://openalex.org/W2091602444","https://openalex.org/W2165184871"],"related_works":["https://openalex.org/W2088368898","https://openalex.org/W2009556993","https://openalex.org/W1981178161","https://openalex.org/W1972072721","https://openalex.org/W7099981613","https://openalex.org/W2101937540","https://openalex.org/W1603840317","https://openalex.org/W2067896999","https://openalex.org/W2041196375","https://openalex.org/W2063371528"],"abstract_inverted_index":{"A":[0],"type":[1],"II":[2],"restriction":[3,69],"endonuclease":[4,70],"designated":[5],"Sse8387I":[6,51,65,94],"was":[7,102],"partially":[8],"purified":[9],"from":[10,45],"Streptomyces":[11],"sp.":[12],"8387.":[13],"This":[14],"enzyme":[15],"cleaved":[16],"adenovirus":[17],"2":[18],"DNA":[19,25,34],"at":[20,26,35],"three":[21],"sites,":[22,28],"lambda":[23],"phage":[24],"five":[27],"and":[29,31,87],"pUC18":[30],"M13mp18":[32],"RF":[33],"one":[36],"site":[37],"each,":[38],"but":[39],"did":[40],"not":[41],"cleave":[42],"the":[43,53,63,67],"DNAs":[44],"pBR322,":[46],"SV40,":[47],"or":[48],"phi":[49],"X174.":[50],"recognized":[52],"octanucleotide":[54,77],"sequence":[55,78],"5'-CCTGCA":[56],"decreases":[57],"GG-3',":[58],"cleaving":[59],"where":[60],"shown":[61],"by":[62],"arrow.":[64],"is":[66],"first":[68],"to":[71],"be":[72],"reported":[73],"that":[74,105],"recognizes":[75],"an":[76],"consisting":[79],"of":[80,91,93,99,106],"all":[81],"four":[82],"nucleotides,":[83],"G,":[84],"A,":[85],"T,":[86],"C.":[88],"The":[89],"frequency":[90],"occurrence":[92],"sites":[95],"within":[96],"sequenced":[97],"regions":[98],"primate":[100],"genomes":[101],"2.4":[103],"times":[104],"NotI":[107],"sites.":[108]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}