{"id":"https://openalex.org/W1968798652","doi":"https://doi.org/10.1097/01.qai.0000351062.72675.7c","title":"105 sCD4-17b, an Engineered Bifunctional HIV-1 Neutralizing Protein: Potent Cross Clade Activity and Potential Microbicide Use","display_name":"105 sCD4-17b, an Engineered Bifunctional HIV-1 Neutralizing Protein: Potent Cross Clade Activity and Potential Microbicide Use","publication_year":2009,"publication_date":"2009-05-25","ids":{"openalex":"https://openalex.org/W1968798652","doi":"https://doi.org/10.1097/01.qai.0000351062.72675.7c","mag":"1968798652"},"language":"en","primary_location":{"id":"doi:10.1097/01.qai.0000351062.72675.7c","doi":"https://doi.org/10.1097/01.qai.0000351062.72675.7c","is_oa":false,"landing_page_url":"https://doi.org/10.1097/01.qai.0000351062.72675.7c","pdf_url":null,"source":{"id":"https://openalex.org/S157460402","display_name":"JAIDS Journal of Acquired Immune Deficiency Syndromes","issn_l":"1525-4135","issn":["1525-4135","1944-7884"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JAIDS Journal of Acquired Immune Deficiency Syndromes","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112732156","display_name":"Laurel A. Lagenaur","orcid":"https://orcid.org/0000-0001-6640-839X","observed_orcids":["https://orcid.org/0000-0001-6640-839X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4210155075","display_name":"Osel (United States)","ror":"https://ror.org/048bsds49","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155075"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Laurel A Lagenaur","raw_affiliation_strings":["NIAID, NIH, Bethesda, MD 20892","Osel, Inc., 4008 Burton Dr., Santa Clara, CA 95054, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, NIH, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"raw_affiliation_string":"Osel, Inc., 4008 Burton Dr., Santa Clara, CA 95054, USA","institution_ids":["https://openalex.org/I4210155075"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031361479","display_name":"Vadim A. Villarroel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vadim A Villarroel","raw_affiliation_strings":["NIAID, NIH, Bethesda, MD 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, NIH, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058743104","display_name":"Edward A. Berger","orcid":"https://orcid.org/0000-0001-8457-3997","observed_orcids":["https://orcid.org/0000-0001-8457-3997"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edward A Berger","raw_affiliation_strings":["NIAID, NIH, Bethesda, MD 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"NIAID, NIH, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.08113288,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"51","issue":"Supplement 2","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10526","display_name":"HIV/AIDS drug development and treatment","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hiv-1-neutralizing-antibodies","display_name":"HIV-1 neutralizing antibodies","score":0.8600000143051147},{"id":"https://openalex.org/keywords/cd4","display_name":"CD4","score":0.42899999022483826},{"id":"https://openalex.org/keywords/linker-length","display_name":"linker length","score":0.6439999938011169},{"id":"https://openalex.org/keywords/cross-clade-neutralization","display_name":"cross-clade neutralization","score":0.8539999723434448},{"id":"https://openalex.org/keywords/broadly-neutralizing-antibodies","display_name":"broadly neutralizing antibodies","score":0.7519999742507935},{"id":"https://openalex.org/keywords/microbicides","display_name":"microbicides","score":0.722000002861023},{"id":"https://openalex.org/keywords/hiv-1-envelope-glycoprotein","display_name":"HIV-1 envelope glycoprotein","score":0.5130000114440918},{"id":"https://openalex.org/keywords/hiv-1-vaccine","display_name":"HIV-1 vaccine","score":0.3140000104904175}],"concepts":[{"id":"https://openalex.org/C2780557392","wikidata":"https://www.wikidata.org/wiki/Q523796","display_name":"Linker","level":2,"score":0.8354020118713379},{"id":"https://openalex.org/C2780443040","wikidata":"https://www.wikidata.org/wiki/Q3737863","display_name":"Bifunctional","level":3,"score":0.6713689565658569},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5619922280311584},{"id":"https://openalex.org/C14086860","wikidata":"https://www.wikidata.org/wiki/Q337120","display_name":"Neutralization","level":3,"score":0.5532573461532593},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.4930674731731415},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.48083505034446716},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.4148585796356201},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.31445685029029846},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28637033700942993},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2862187623977661},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.2860979735851288},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.22469276189804077},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11904439330101013},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1097/01.qai.0000351062.72675.7c","doi":"https://doi.org/10.1097/01.qai.0000351062.72675.7c","is_oa":false,"landing_page_url":"https://doi.org/10.1097/01.qai.0000351062.72675.7c","pdf_url":null,"source":{"id":"https://openalex.org/S157460402","display_name":"JAIDS Journal of Acquired Immune Deficiency Syndromes","issn_l":"1525-4135","issn":["1525-4135","1944-7884"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JAIDS Journal of Acquired Immune Deficiency Syndromes","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2152089409","https://openalex.org/W2012451048","https://openalex.org/W1988781661","https://openalex.org/W2156997818","https://openalex.org/W2132847251","https://openalex.org/W2093772830","https://openalex.org/W2760713071","https://openalex.org/W3182005667","https://openalex.org/W2102670802","https://openalex.org/W2150412330"],"abstract_inverted_index":{"We":[0,126],"previously":[1,304],"described":[2],"sCD4-17b,":[3],"an":[4],"engineered":[5],"single":[6],"chain":[7],"bifunctional":[8],"protein":[9],"that":[10,74,357],"neutralizes":[11],"HIV-1":[12,149,254],"infection":[13,300,342],"(Dey":[14],"et":[15,67,315],"al.,":[16,68,316],"J.":[17],"Virol.,":[18],"2003).":[19],"sCD4-17b":[20,128,161,239,324,355],"contains":[21],"the":[22,41,51,63,83,92,156,207,221,235,241,281],"first":[23],"two":[24],"domains":[25],"of":[26,54,91,120,132,165,182,198,206,337,354],"CD4":[27],"attached":[28],"by":[29,323],"a":[30,34,55,75,118,177,180,370],"flexible":[31],"linker":[32,78,181,244],"to":[33,59,104,110,117,212,291,306,309,333,372],"17b":[35,64,95,124,141,202,210,229],"mAb":[36],"scFv,":[37],"whose":[38],"epitope":[39],"overlaps":[40],"CD4i":[42],"bridging":[43],"sheet":[44],"involved":[45],"in":[46,155,278,343],"coreceptor":[47],"binding.":[48],"Based":[49],"on":[50,219],"crystal":[52],"structure":[53],"gp120":[56,214],"core":[57],"complexed":[58],"two-domain":[60],"sCD4":[61,93,122,139,200,208,227],"and":[62,94,123,140,209,262,273,352],"Fab":[65],"(Kwong":[66],"Nature,":[69],"1998),":[70],"we":[71],"originally":[72],"anticipated":[73],"35":[76,166],"aa":[77,169,185,243],"length":[79],"would":[80],"readily":[81],"span":[82],"56":[84],"\u00c5":[85],"distance":[86],"required":[87],"for":[88,359],"simultaneous":[89,106],"binding":[90,107],"moieties,":[96],"thus":[97],"neutralizing":[98,312],"free":[99,138],"virions.":[100],"Linkers":[101],"too":[102],"short":[103],"enable":[105],"were":[108,146,283,321],"predicted":[109],"be":[111,307],"much":[112],"less":[113,188],"active,":[114],"perhaps":[115],"equivalent":[116],"mixture":[119],"unlinked":[121,199],"scFv.":[125,142],"expressed":[127],"constructs":[129,162],"containing":[130,151],"linkers":[131,164],"various":[133],"lengths,":[134],"as":[135,137,175,265,267,369],"well":[136,266],"The":[143,349],"purified":[144],"proteins":[145],"tested":[147,246],"against":[148,247],"pseudotypes":[150,248],"genetically":[152,252],"diverse":[153,253],"Envs":[154,250,257,297],"TZM-bl":[157],"single-round":[158],"neutralization":[159,173,275],"assay.":[160],"with":[163,179,240,249,326],"or":[167,223],"40":[168,242],"displayed":[170],"equivalently":[171],"potent":[172],"activities;":[174],"expected,":[176],"construct":[178],"only":[183],"5":[184],"was":[186,192,245,276],"significantly":[187],"potent,":[189],"although":[190],"it":[191],"more":[193],"effective":[194],"than":[195],"equimolar":[196],"concentrations":[197],"plus":[201,228],"(Perhaps":[203],"reflecting":[204],"cross-binding":[205],"moieties":[211],"different":[213,217,224],"subunits,":[215],"between":[216],"spikes":[218],"either":[220],"same":[222,236],"virions).":[225],"Unlinked":[226],"scFv":[230],"had":[231],"minimal":[232],"activities":[233],"over":[234],"concentration":[237],"ranges.":[238],"from":[251,258,269,298],"isolates.":[255],"Amongst":[256,296],"standardized":[259],"clade":[260],"B":[261],"C":[263],"panels,":[264],"several":[268],"clades":[270],"D,":[271],"E,":[272],"F,":[274],"observed":[277],"every":[279],"case;":[280],"potencies":[282],"consistently":[284],"high":[285],"(IC50":[286],"2":[287,294],"-":[288,293],"40nm,":[289],"corresponding":[290,332],"0.1":[292],"\u03bcg/mL).":[295,335],"early":[299],"subtype":[301],"A":[302],"isolates":[303],"shown":[305],"resistant":[308],"most":[310],"broadly":[311],"mAbs":[313],"(Blish":[314],"AIDS":[317],"2007),":[318],"nearly":[319],"all":[320],"neutralized":[322],"albeit":[325],"higher":[327],"IC50":[328],"values":[329],"(40-200":[330],"nM,":[331],"2-10":[334],"Neutralization":[336],"\u201clive\u201d":[338],"virus":[339],"via":[340],"spreading":[341],"PBMC's":[344],"is":[345],"currently":[346],"being":[347],"examined.":[348],"combined":[350],"breadth":[351],"potency":[353],"exceeds":[356],"reported":[358],"any":[360],"anti-HIV":[361],"mAbs.":[362],"These":[363],"findings":[364],"suggest":[365],"its":[366],"potential":[367],"use":[368],"microbicide":[371],"prevent":[373],"sexual":[374],"transmission.":[375]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}